Gene Details:

Functional Descriptions:

  • The expression of water channel protein RWC3 mRNA was increased in upland rice at the early response (up to 4 h) to the 20% polyethylene glycol (PEG) 6000 treatment, whereas there was no significant expression changes in lowland rice.
  • Partial silencing of the water channel genes, OsPIP1;1 and OsPIP1;3, reduced seed germination while over-expression of OsPIP1;3 promoted seed germination under water-stress conditions.
  • Moreover, spatial expression analysis indicates that OsPIP1;3 is expressed predominantly in embryo during seed germination.
  • Exogenous NO stimulated the transcription of OsPIP1;1, OsPIP1;2, OsPIP1;3 and OsPIP2;8 in germinating seeds.
  • The up-regulation of RWC3 in upland rice fits well with the knowledge that upland rice adopts the mechanism of drought avoidance.
  • These results indicated that RWC3 probably played a role in drought avoidance in rice.
  • The role of aquaporin RWC3 in drought avoidance in rice.
  • Immunocytochemistry showed that OsPIP1;3 majorly accumulated on the proximal end of the endodermis and the cell surface around xylem.
  • Further experiment suggested that heterologous expression of OsPIP1;3 in cyanobacterium promoted bacterial growth in the condition with different CO2 gas supply.
  • Here, from a drought-resistant rice cultivar, we isolated an OsPIP1;3 gene SNP which is mostly expressed in rice roots, and is strongly responsive to drought stress.
  • Expression of GFP-OsPIP1;3 alone in Xenopus oocytes or rice protoplasts showed OsPIP1;3 mislocalization in ER-like neighborhood, whereas co-expression of OsPIP2;2 recruited OsPIP1;3 to the plasma membrane and led to a significant enhancement of water permeability in oocytes.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os02g57720.1
    GTGGAGCAGGAGCTATAAAAAGAAGCAGGAAGCCACTCGCTCTCCTCACAGCCACAGCTCGCCATCGTCGTCGTCGTCGTCGTCGTCTCCGGCTCCGGCAAGAGAGCTAGTAGTGAGTAGTAGAAAGAAGAAGAGGCAAATTAATAGTAATGGAGGGGAAGGAGGAGGATGTGAGGCTGGGGGCGAACAGGTACACGGAGAGGCAGCCGATCGGGACGGCGGCGCAGGGGGCGGAGGAGAAGGACTACCGGGAGCCGCCGGCGGCGCCGGTGTTCGAGGTGGAGGAGCTGACGTCGTGGTCGTTCTACCGGGCGGGGATCGCGGAGTTCGTGGCGACGTTCCTGTTCCTGTACATCAGCATCCTGACGGTGATGGGGGTGAACAAGTCGGCGTCCAAGTGCGCCACCGTGGGGATCCAGGGCATCGCGTGGTCGTTCGGCGGCATGATCTTCGCGCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACGTTCGGGCTGTTCCTGGCGCGGAAGCTGTCGCTGACGCGGGCGGTGTTCTACATGGCGATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCGGGGGTTGTACATGGGCTCCGGCGGCGGCGCCAACGCCGTGAACCCGGGGTACACCAAGGGGGACGGGCTCGGGGCGGAGATCGTGGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGCGCAACGCCAGGGACTCCCACGTCCCCATCCTGGCGCCGCTCCCCATCGGCTTCGCCGTCTTCCTCGTCCACCTCGCCACCATCCCCATCACCGGCACCGGCATCAACCCCGCCCGCAGCCTCGGCGCCGCCATCGTCTATAACCGCGCCCACGCATGGCACGACCACTGGATTTTCTGGGTTGGTCCGTTCATCGGAGCGGCACTGGCGGCCATCTACCACGTGGTGGTGATCAGAGCAATCCCCTTCAAGAGCCGGGACTAATTAATTACCATGCACCATCATCTTAATTAAGCGATAATTACTCTGAAATAATCAACCGAGTTGATGCTGCAAGATGATGGAGGATCAAGTCTCTACAGTACTACGTGTGTTATTACCGTCTGGTGATCGATGAAGCTAGCCGCTGCATAATTATAAGCGCGTTTATATATATCTGATGTTGCTGTAATTATATCTATGTGTACGTAGTATGTTTGGTGTGAGAAATGGTACTTGTGTGTGCGGAGGCATGCATGTTCTTGGTTGCACGCCTTCAGCTATATATAGCTTTGCATCAATTCCATCTCCAAATGTGAATTTTACTAGTTACTATTTTTACCTCCTCTCATCCCCTCTTATGTTATGTGTGATCAAGTATTTGATCCCCTCTCGTTAAGTAAATGTTAAGCATGCGTATTTGTAAACTTGTTAGTGCTATACTGATATGCTATCGGCCCTATCGGCGCCATTATTGCCGGGTCTTCAAATGCCACGGTGTATGAGAAGACTTACGAATTGTGCAAAAAAAAAAAAAA
CDS Sequence
  • >LOC_Os02g57720.1
    ATGGAGGGGAAGGAGGAGGATGTGAGGCTGGGGGCGAACAGGTACACGGAGAGGCAGCCGATCGGGACGGCGGCGCAGGGGGCGGAGGAGAAGGACTACCGGGAGCCGCCGGCGGCGCCGGTGTTCGAGGTGGAGGAGCTGACGTCGTGGTCGTTCTACCGGGCGGGGATCGCGGAGTTCGTGGCGACGTTCCTGTTCCTGTACATCAGCATCCTGACGGTGATGGGGGTGAACAAGTCGGCGTCCAAGTGCGCCACCGTGGGGATCCAGGGCATCGCGTGGTCGTTCGGCGGCATGATCTTCGCGCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACGTTCGGGCTGTTCCTGGCGCGGAAGCTGTCGCTGACGCGGGCGGTGTTCTACATGGCGATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCGGGGGTTGTACATGGGCTCCGGCGGCGGCGCCAACGCCGTGAACCCGGGGTACACCAAGGGGGACGGGCTCGGGGCGGAGATCGTGGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGCGCAACGCCAGGGACTCCCACGTCCCCATCCTGGCGCCGCTCCCCATCGGCTTCGCCGTCTTCCTCGTCCACCTCGCCACCATCCCCATCACCGGCACCGGCATCAACCCCGCCCGCAGCCTCGGCGCCGCCATCGTCTATAACCGCGCCCACGCATGGCACGACCACTGGATTTTCTGGGTTGGTCCGTTCATCGGAGCGGCACTGGCGGCCATCTACCACGTGGTGGTGATCAGAGCAATCCCCTTCAAGAGCCGGGACTAA
Protein Sequence
  • >LOC_Os02g57720.1
    MEGKEEDVRLGANRYTERQPIGTAAQGAEEKDYREPPAAPVFEVEELTSWSFYRAGIAEFVATFLFLYISILTVMGVNKSASKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYMAMQCLGAICGAGVVKGFQRGLYMGSGGGANAVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIVYNRAHAWHDHWIFWVGPFIGAALAAIYHVVVIRAIPFKSRD*