Information report for RWC3;OsPIP1;3
Gene Details
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Functional Descriptions
- The expression of water channel protein RWC3 mRNA was increased in upland rice at the early response (up to 4 h) to the 20% polyethylene glycol (PEG) 6000 treatment, whereas there was no significant expression changes in lowland rice.
- Partial silencing of the water channel genes, OsPIP1;1 and OsPIP1;3, reduced seed germination while over-expression of OsPIP1;3 promoted seed germination under water-stress conditions.
- Moreover, spatial expression analysis indicates that OsPIP1;3 is expressed predominantly in embryo during seed germination.
- Exogenous NO stimulated the transcription of OsPIP1;1, OsPIP1;2, OsPIP1;3 and OsPIP2;8 in germinating seeds.
- The up-regulation of RWC3 in upland rice fits well with the knowledge that upland rice adopts the mechanism of drought avoidance.
- These results indicated that RWC3 probably played a role in drought avoidance in rice.
- The role of aquaporin RWC3 in drought avoidance in rice.
- Immunocytochemistry showed that OsPIP1;3 majorly accumulated on the proximal end of the endodermis and the cell surface around xylem.
- Further experiment suggested that heterologous expression of OsPIP1;3 in cyanobacterium promoted bacterial growth in the condition with different CO2 gas supply.
- Here, from a drought-resistant rice cultivar, we isolated an OsPIP1;3 gene SNP which is mostly expressed in rice roots, and is strongly responsive to drought stress.
- Expression of GFP-OsPIP1;3 alone in Xenopus oocytes or rice protoplasts showed OsPIP1;3 mislocalization in ER-like neighborhood, whereas co-expression of OsPIP2;2 recruited OsPIP1;3 to the plasma membrane and led to a significant enhancement of water permeability in oocytes.
Functional Keywords
- ethylene , seed , drought , seed-germination , xylem , growth , stress , plasma-membrane , drought-stress
Literature and News
- The role of water channel proteins and nitric oxide signaling in rice seed germination . DOI: 10.1038/cr.2007.34 ; PMID: 17452999
- The role of aquaporin RWC3 in drought avoidance in rice . DOI: 10.1093/pcp/pch058 ; PMID: 15111723
- Rice aquaporin PIP1;3 and harpin Hpa1 of bacterial blight pathogen cooperate in a type III effector translocation . DOI: 10.1093/jxb/erz130 ; PMID: 30921464
- Ectopic expression of a rice plasma membrane intrinsic protein (OsPIP1;3) promotes plant growth and water uptake . DOI: 10.1111/tpj.14662 ; PMID: 31872463
Gene Resources
- UniProt: Q9SXF8
- EMBL: AB016623, AB029325, AK102174
- AlphaFoldDB: Q9SXF8
- EnsemblPlants: Os02t0823100-01
- Gramene: Os02t0823100-01
- KEGG: dosa:Os02g0823100
- Orthologous matrix: WDPVNKE
- InterPro: IPR000425, IPR022357, IPR023271
Homologs
Sequences
cDNA Sequence
- >LOC_Os02g57720.1
GTGGAGCAGGAGCTATAAAAAGAAGCAGGAAGCCACTCGCTCTCCTCACAGCCACAGCTCGCCATCGTCGTCGTCGTCGTCGTCGTCTCCGGCTCCGGCAAGAGAGCTAGTAGTGAGTAGTAGAAAGAAGAAGAGGCAAATTAATAGTAATGGAGGGGAAGGAGGAGGATGTGAGGCTGGGGGCGAACAGGTACACGGAGAGGCAGCCGATCGGGACGGCGGCGCAGGGGGCGGAGGAGAAGGACTACCGGGAGCCGCCGGCGGCGCCGGTGTTCGAGGTGGAGGAGCTGACGTCGTGGTCGTTCTACCGGGCGGGGATCGCGGAGTTCGTGGCGACGTTCCTGTTCCTGTACATCAGCATCCTGACGGTGATGGGGGTGAACAAGTCGGCGTCCAAGTGCGCCACCGTGGGGATCCAGGGCATCGCGTGGTCGTTCGGCGGCATGATCTTCGCGCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACGTTCGGGCTGTTCCTGGCGCGGAAGCTGTCGCTGACGCGGGCGGTGTTCTACATGGCGATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCGGGGGTTGTACATGGGCTCCGGCGGCGGCGCCAACGCCGTGAACCCGGGGTACACCAAGGGGGACGGGCTCGGGGCGGAGATCGTGGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGCGCAACGCCAGGGACTCCCACGTCCCCATCCTGGCGCCGCTCCCCATCGGCTTCGCCGTCTTCCTCGTCCACCTCGCCACCATCCCCATCACCGGCACCGGCATCAACCCCGCCCGCAGCCTCGGCGCCGCCATCGTCTATAACCGCGCCCACGCATGGCACGACCACTGGATTTTCTGGGTTGGTCCGTTCATCGGAGCGGCACTGGCGGCCATCTACCACGTGGTGGTGATCAGAGCAATCCCCTTCAAGAGCCGGGACTAATTAATTACCATGCACCATCATCTTAATTAAGCGATAATTACTCTGAAATAATCAACCGAGTTGATGCTGCAAGATGATGGAGGATCAAGTCTCTACAGTACTACGTGTGTTATTACCGTCTGGTGATCGATGAAGCTAGCCGCTGCATAATTATAAGCGCGTTTATATATATCTGATGTTGCTGTAATTATATCTATGTGTACGTAGTATGTTTGGTGTGAGAAATGGTACTTGTGTGTGCGGAGGCATGCATGTTCTTGGTTGCACGCCTTCAGCTATATATAGCTTTGCATCAATTCCATCTCCAAATGTGAATTTTACTAGTTACTATTTTTACCTCCTCTCATCCCCTCTTATGTTATGTGTGATCAAGTATTTGATCCCCTCTCGTTAAGTAAATGTTAAGCATGCGTATTTGTAAACTTGTTAGTGCTATACTGATATGCTATCGGCCCTATCGGCGCCATTATTGCCGGGTCTTCAAATGCCACGGTGTATGAGAAGACTTACGAATTGTGCAAAAAAAAAAAAAA
CDS Sequence
- >LOC_Os02g57720.1
ATGGAGGGGAAGGAGGAGGATGTGAGGCTGGGGGCGAACAGGTACACGGAGAGGCAGCCGATCGGGACGGCGGCGCAGGGGGCGGAGGAGAAGGACTACCGGGAGCCGCCGGCGGCGCCGGTGTTCGAGGTGGAGGAGCTGACGTCGTGGTCGTTCTACCGGGCGGGGATCGCGGAGTTCGTGGCGACGTTCCTGTTCCTGTACATCAGCATCCTGACGGTGATGGGGGTGAACAAGTCGGCGTCCAAGTGCGCCACCGTGGGGATCCAGGGCATCGCGTGGTCGTTCGGCGGCATGATCTTCGCGCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACGTTCGGGCTGTTCCTGGCGCGGAAGCTGTCGCTGACGCGGGCGGTGTTCTACATGGCGATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCGGGGGTTGTACATGGGCTCCGGCGGCGGCGCCAACGCCGTGAACCCGGGGTACACCAAGGGGGACGGGCTCGGGGCGGAGATCGTGGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGCGCAACGCCAGGGACTCCCACGTCCCCATCCTGGCGCCGCTCCCCATCGGCTTCGCCGTCTTCCTCGTCCACCTCGCCACCATCCCCATCACCGGCACCGGCATCAACCCCGCCCGCAGCCTCGGCGCCGCCATCGTCTATAACCGCGCCCACGCATGGCACGACCACTGGATTTTCTGGGTTGGTCCGTTCATCGGAGCGGCACTGGCGGCCATCTACCACGTGGTGGTGATCAGAGCAATCCCCTTCAAGAGCCGGGACTAA
Protein Sequence
- >LOC_Os02g57720.1
MEGKEEDVRLGANRYTERQPIGTAAQGAEEKDYREPPAAPVFEVEELTSWSFYRAGIAEFVATFLFLYISILTVMGVNKSASKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYMAMQCLGAICGAGVVKGFQRGLYMGSGGGANAVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIVYNRAHAWHDHWIFWVGPFIGAALAAIYHVVVIRAIPFKSRD*