Information report for OsPHO1;2
Gene Details
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Functional Descriptions
- Characterization of Ospho1;1 and OsPHO1;2 insertion mutants revealed that only OsPHO1;2 mutants had defects in Pi homeostasis, namely strong reduction in Pi transfer from root to shoot, which was accompanied by low-shoot and high-root Pi.
- Our data identify OsPHO1;2 as playing a key role in the transfer of Pi from roots to shoots in rice, and indicate that this gene could be regulated by its cis-natural antisense transcripts.
- Characterization of the rice PHO1 gene family reveals a key role for OsPHO1;2 in phosphate homeostasis and the evolution of a distinct clade in dicotyledons.
- However, while the OsPHO1;2 sense transcript was relatively stable under various nutrient deficiencies, the antisense transcript was highly induced by inorganic phosphate (Pi) deficiency.
- The most abundantly expressed gene was OsPHO1;2 in the roots, for both sense and antisense transcripts.
- OsPHO1;1 was mainly localized at the phloem region of diffuse vascular bundles of node I, while OsPHO1;2 was expressed in the xylem parenchyma cells of the enlarged vascular bundles.
- Taken together, OsPHO1;2 expressed in node I is responsible for unloading of P from the xylem of enlarged vascular bundles, while OsPHO1;1 is involved in reloading P into phloem of diffuse vascular bundles for subsequent allocation of P to the seeds.
- At the reproductive stage, both OsPHO1;1 and OsPHO1;2 showed higher expression in the node I, the uppermost node connecting to panicle.
- Furthermore, OsPHO1;1 and OsPHO1;2 expressed in the caryopsis are important for delivering of P from the maternal tissues to the filial tissues for seed development.
- Knockout of OsPHO1;2 as well as OsPHO1;1 with less extent decreased the distribution of P to the seed, resulting in decreased seed size and delayed germination.
- Both OsPHO1;1 and OsPHO1;2 were localized to the plasma membrane and showed influx transport activities for inorganic phosphate.
Functional Keywords
- shoot , pi , homeostasis , phosphate , root , xylem , vascular-bundle , panicle , development , seed , reproductive , phloem , seed-size , plasma-membrane , seed-development , node , xylem-parenchyma
Literature and News
- Characterization of the rice PHO1 gene family reveals a key role for OsPHO1;2 in phosphate homeostasis and the evolution of a distinct clade in dicotyledons . DOI: 10.1104/pp.109.149872 ; PMID: 20081045
- Node-Localized Transporters of Phosphorus Essential for Seed Development in Rice . DOI: 10.1093/pcp/pcaa074 ; PMID: 32484878
Gene Resources
- UniProt: Q6K991
- EMBL: AK100323, AP003996, AP004064
- AlphaFoldDB: Q6K991
- EnsemblPlants: Os02t0809800-01
- Gramene: Os02t0809800-01
- KEGG: dosa:Os02g0809800
- Orthologous matrix: ETSHFYT
Sequences
cDNA Sequence
- >LOC_Os02g56510.1
GGTCGATCCCCACTATGTAAGTGTATATATATATCTCTGAATTCAGATACACCTTTCTACTTTAATTTAGCTAAGCTAGCTAGCTCTACCTAATATTGTAACATACAAGGCCACTATCTGTCCCTGTATGTGCACCAGCAGTGCCAAATTTCGCTGCATGCTTAGCTAAGCTAAGCTTATATAGCAGGAGCACTCGAGGTGAGCAGAGAGAGAGGATTGAAGAAGAAGGAGAAGAGCACCAAGCTAGTAGCCTGATCTCTCTCTCTCTCTCTAACTGTCTATCTATATAAGCTAACAAGGCAGGACGGTTGTTGCTTGCGAGATCATCCCTGCCTGCCTGTCCCTTTCCTGCATCCATCCATCCATCTTGATCTTCTCCTAGCTCGATTCCACACATGTGAACGTACGTACGTTTCAGTTAATTGTTCGATCGATCGATCGATCAGCTGCTGCTGCTGCTGCTGCAACGAGGAAGAAGAAGAAGAAGAAGATGGTGAAGTTCTCGAGGGAGTACGAGGCGAGCATCATACCGGAGTGGAAGGCTGCGTTCGTCGACTACAAGCGGCTCAAGAAGCTCATCAAGAGGATCAAGGTCACCCGCCGCGACGACTCCTTCGCCGCCGCCAACGCCGCAGCCGCAGCCGACCACCTTCTCCCGCCGCCGCCCGCAGAGAAGGAAGCCGGTGGCTATGGGTTCTCCATTCTTGACCCCGTTCGCGCCATCGCCGCCCGCTTCTCCGCTGGACAACAGCCTTCTGCCTCAGAGGATGAGGAGTGCCCGGACAGAGGGGAGCTCGTGCGATCCACGGACAAGCATGAGAGGGAGTTCATGGAGAGGGCGGACGAGGAGCTGGAGAAGGTGAACGCGTTCTACACGGGGCAGGAGGCGGAGCTGCTGGCGCGCGGCGACGCGCTGCTGGAGCAGCTCCGGATCCTCGCCGACGTCAAGCGCATCCTCGCCGACCACGCCGCCGCCCGCCGCGCCCGCGGCCTCGCCCGAAGCCGCTCTATGCCGCCGCCGCCGCCGTCCTCCTCGCCGCCGTCGTCGGTGCACGGCTCCAGCGGCCGCTACCTCCTCTCCGGCCTCTCCTCGCCGCAGTCCATGTCAGATGGGAGCCTGGAGTTGCAGCAGGCGCAGGTGTCGGAGGGCGCGGCGGTGGCGGACGAGGTGATGGCGGCGCTGGAGCGGAACGGCGTCAGCTTCGTGGGGCTCGCCGGGAAGAAGGACGGCAAGACCAAGGACGGCAGCGGCAAAGGGCGGGGCGGCGGCGGCGGCGGAGGCGGAGGCGTGCTGCAGCTGCCAGCGACGGTGAGGATCGACATCCCGGCGACGAGCCCCGGGCGGGCGGCGCTCAAGGTGTGGGAGGAGCTCGTGAACGTGCTCCGCAAGGACGGCGCCGACCCCGCCGCGGCGTTCGTCCACCGGAAGAAGATCCAGCACGCCGAGAAGAACATCCGCGACGCCTTCATGGCGCTCTACCGCGGCCTCGAGCTGCTCAAGAAATTCAGCTCTCTCAATGTAAAGGCTTTCACCAAAATATTGAAGAAATTTGTCAAGGTGTCCGAGCAACAGCGAGCTACAGACCTGTTTTCAGAGAAGGTGAAGAGGTCGCCATTTAGCAGCTCCGACAAGGTACTTCAGCTAGCAGATGAGGTCGAGTGCATCTTCATGAAGCATTTCACAGGCAATGACAGGAAGGTGGCGATGAAATACCTTAAGCCACAGCAGCCCAGAAACACTCACATGATCACCTTCCTCGTAGGTTTGTTCACGGGCACTTTTGTTTCACTGTTCATCATATACGCGATTTTAGCCCATGTTAGTGGTATTTTTACTTCCACTGGGAACTCAGCCTACATGGAAATTGTGTACCATGTTTTCAGCATGTTTGCTCTCATCAGCCTCCACATCTTCCTGTATGGGTGCAACCTCTTCATGTGGAAGAACACAAGGATCAATCACAACTTCATATTCGATTTCTCCTCAAACACCGCCTTGACGCACCGGGACGCCTTCCTCATGTCTGCATCGATAATGTGCACTGTTGTCGCTGCACTGGTCATCAATCTTTTCCTCAAGAACGCCGGTGTAGCCTATGCCAATGCTTTGCCTGGAGCACTCCTACTACTGTCAACAGGTGTACTATTCTGTCCATTTGACATATTCTACCGCTCGACGCGATACTGCTTCATGCGTGTCATGCGCAATATCATATTTTCACCATTCTATAAGGTTCTGATGGCTGATTTCTTCATGGCTGACCAACTAACTAGCCAGATTCCACTATTACGGCACATGGAATTTACAGCGTGCTACTTCATGGCTGGAAGCTTTAGAACTCACCCATATGAGACCTGTACTAGTGGCCAACAATATAAGCATCTGGCCTATGTGATCTCCTTTCTCCCATACTTCTGGAGAGCCTTGCAGTGTTTAAGAAGGTACTTGGAGGAAGGACATGATATCAACCAACTTGCTAATGCTGGAAAATATGTATCAGCAATGGTAGCAGCTGCTGTTAGGTTCAAATATGCTGCAACACCAACACCATTCTGGGTGTGGATGGTCATCATTTCATCCTCAGGTGCCACAATTTACCAGCTCTACTGGGACTTTGTCAAGGACTGGGGCTTCTTAAACCCAAAATCAAAGAACCGATGGCTTCGTAACGAGCTCATCTTGAAGAATAAATCAATCTACTACGTATCCATGATGCTCAATCTTGCACTTCGCCTAGCATGGACTGAGAGTGTAATGAAGATCCATATTGGTAAGGTTGAGTCGCGCTTACTGGATTTCTCGCTCGCTTCACTAGAGATAATCAGACGAGGCCATTGGAACTTCTATAGATTGGAGAATGAACATTTGAACAACGTTGGCAAGTTCAGAGCAGTGAAGACTGTCCCATTACCATTCCGTGAACTTGAAACTGATTGATTAAGCAGTAGGACATGCCGGCATGCTGCCAACATCAAGGCCAGTAAGGATCAGAGTTCCAGGAATGGAGATGCTGCTTGCCGCTAGATTTGGTACATAAGTGGTAATAAAGTTTGCAGAGACCATTGGTGCACAAGACCATGATGTTGCTGATTAGGATGGAATAAGCTTAAATCAGAGCAGCTAATCGAGGCAGTTCGTCAAAAAAGAAGGAATAATCCCTTCTTGGTCCGATGAAGATTGTTGGGCGTCATTTCGCTAACAAGATCTTAATTTTGTTTATGGTTGTGTTATGTATGTATCTGGAGATGGATGCCCACCCTCCAAAAAAAATATTCCATGAATTCATATGCACCATTGCATCAAACTGAATTCAAATAATTTGCCGATAAACAAAATAAGTAAGATGTCAATGTTAGAGAAGGAAATAAGAACACAATGGGGTAAAATAAACATGCAATAGATATTAATTAACTGAAGGGGAAGAAACCCAATCATTTCTGCTAATACAGTGAAAGCAAGATGCTTGGCACTACAGATGACTAAATAAGCTAAAGCATAAACATAGTACAAATATATTACATGTCGATCACATGATGCACACGATAAATAGATAGCTATGACAGATACCTCCATTGTGCTGGACGGTCTCTTGAGACAAGAAGAACCCTTGATGACGAGTAATCGAATGTAGGGCAGTACAAATCATCAAAGCCAATAGCCTCTCTTACTCCAAGAGTGGCTCCACGGTCAAAGCTGTAAGTTTGTTGACCGTCCTCCTTAAAGGCGTCAAATGCAAATAGTCCCATACCACATGCAGCCAAGACAAGGTTGGAGTTGCCCCATACTTGGGTTATTGAAGAGTGATGGAAGTGGGCATTGAAATCAGGTAGCTCATAGGTTGCAACAAGCTTTCCCTTTCTCAGTGAATATTGCTGAACTCGTGATTTCAGACCCCCTTTTATTTGCAGCCTACCTTCTGTGCTCCCAATGAATACCGAGTCACC
CDS Sequence
- >LOC_Os02g56510.1
ATGGTGAAGTTCTCGAGGGAGTACGAGGCGAGCATCATACCGGAGTGGAAGGCTGCGTTCGTCGACTACAAGCGGCTCAAGAAGCTCATCAAGAGGATCAAGGTCACCCGCCGCGACGACTCCTTCGCCGCCGCCAACGCCGCAGCCGCAGCCGACCACCTTCTCCCGCCGCCGCCCGCAGAGAAGGAAGCCGGTGGCTATGGGTTCTCCATTCTTGACCCCGTTCGCGCCATCGCCGCCCGCTTCTCCGCTGGACAACAGCCTTCTGCCTCAGAGGATGAGGAGTGCCCGGACAGAGGGGAGCTCGTGCGATCCACGGACAAGCATGAGAGGGAGTTCATGGAGAGGGCGGACGAGGAGCTGGAGAAGGTGAACGCGTTCTACACGGGGCAGGAGGCGGAGCTGCTGGCGCGCGGCGACGCGCTGCTGGAGCAGCTCCGGATCCTCGCCGACGTCAAGCGCATCCTCGCCGACCACGCCGCCGCCCGCCGCGCCCGCGGCCTCGCCCGAAGCCGCTCTATGCCGCCGCCGCCGCCGTCCTCCTCGCCGCCGTCGTCGGTGCACGGCTCCAGCGGCCGCTACCTCCTCTCCGGCCTCTCCTCGCCGCAGTCCATGTCAGATGGGAGCCTGGAGTTGCAGCAGGCGCAGGTGTCGGAGGGCGCGGCGGTGGCGGACGAGGTGATGGCGGCGCTGGAGCGGAACGGCGTCAGCTTCGTGGGGCTCGCCGGGAAGAAGGACGGCAAGACCAAGGACGGCAGCGGCAAAGGGCGGGGCGGCGGCGGCGGCGGAGGCGGAGGCGTGCTGCAGCTGCCAGCGACGGTGAGGATCGACATCCCGGCGACGAGCCCCGGGCGGGCGGCGCTCAAGGTGTGGGAGGAGCTCGTGAACGTGCTCCGCAAGGACGGCGCCGACCCCGCCGCGGCGTTCGTCCACCGGAAGAAGATCCAGCACGCCGAGAAGAACATCCGCGACGCCTTCATGGCGCTCTACCGCGGCCTCGAGCTGCTCAAGAAATTCAGCTCTCTCAATGTAAAGGCTTTCACCAAAATATTGAAGAAATTTGTCAAGGTGTCCGAGCAACAGCGAGCTACAGACCTGTTTTCAGAGAAGGTGAAGAGGTCGCCATTTAGCAGCTCCGACAAGGTACTTCAGCTAGCAGATGAGGTCGAGTGCATCTTCATGAAGCATTTCACAGGCAATGACAGGAAGGTGGCGATGAAATACCTTAAGCCACAGCAGCCCAGAAACACTCACATGATCACCTTCCTCGTAGGTTTGTTCACGGGCACTTTTGTTTCACTGTTCATCATATACGCGATTTTAGCCCATGTTAGTGGTATTTTTACTTCCACTGGGAACTCAGCCTACATGGAAATTGTGTACCATGTTTTCAGCATGTTTGCTCTCATCAGCCTCCACATCTTCCTGTATGGGTGCAACCTCTTCATGTGGAAGAACACAAGGATCAATCACAACTTCATATTCGATTTCTCCTCAAACACCGCCTTGACGCACCGGGACGCCTTCCTCATGTCTGCATCGATAATGTGCACTGTTGTCGCTGCACTGGTCATCAATCTTTTCCTCAAGAACGCCGGTGTAGCCTATGCCAATGCTTTGCCTGGAGCACTCCTACTACTGTCAACAGGTGTACTATTCTGTCCATTTGACATATTCTACCGCTCGACGCGATACTGCTTCATGCGTGTCATGCGCAATATCATATTTTCACCATTCTATAAGGTTCTGATGGCTGATTTCTTCATGGCTGACCAACTAACTAGCCAGATTCCACTATTACGGCACATGGAATTTACAGCGTGCTACTTCATGGCTGGAAGCTTTAGAACTCACCCATATGAGACCTGTACTAGTGGCCAACAATATAAGCATCTGGCCTATGTGATCTCCTTTCTCCCATACTTCTGGAGAGCCTTGCAGTGTTTAAGAAGGTACTTGGAGGAAGGACATGATATCAACCAACTTGCTAATGCTGGAAAATATGTATCAGCAATGGTAGCAGCTGCTGTTAGGTTCAAATATGCTGCAACACCAACACCATTCTGGGTGTGGATGGTCATCATTTCATCCTCAGGTGCCACAATTTACCAGCTCTACTGGGACTTTGTCAAGGACTGGGGCTTCTTAAACCCAAAATCAAAGAACCGATGGCTTCGTAACGAGCTCATCTTGAAGAATAAATCAATCTACTACGTATCCATGATGCTCAATCTTGCACTTCGCCTAGCATGGACTGAGAGTGTAATGAAGATCCATATTGGTAAGGTTGAGTCGCGCTTACTGGATTTCTCGCTCGCTTCACTAGAGATAATCAGACGAGGCCATTGGAACTTCTATAGATTGGAGAATGAACATTTGAACAACGTTGGCAAGTTCAGAGCAGTGAAGACTGTCCCATTACCATTCCGTGAACTTGAAACTGATTGA
Protein Sequence
- >LOC_Os02g56510.1
MVKFSREYEASIIPEWKAAFVDYKRLKKLIKRIKVTRRDDSFAAANAAAAADHLLPPPPAEKEAGGYGFSILDPVRAIAARFSAGQQPSASEDEECPDRGELVRSTDKHEREFMERADEELEKVNAFYTGQEAELLARGDALLEQLRILADVKRILADHAAARRARGLARSRSMPPPPPSSSPPSSVHGSSGRYLLSGLSSPQSMSDGSLELQQAQVSEGAAVADEVMAALERNGVSFVGLAGKKDGKTKDGSGKGRGGGGGGGGGVLQLPATVRIDIPATSPGRAALKVWEELVNVLRKDGADPAAAFVHRKKIQHAEKNIRDAFMALYRGLELLKKFSSLNVKAFTKILKKFVKVSEQQRATDLFSEKVKRSPFSSSDKVLQLADEVECIFMKHFTGNDRKVAMKYLKPQQPRNTHMITFLVGLFTGTFVSLFIIYAILAHVSGIFTSTGNSAYMEIVYHVFSMFALISLHIFLYGCNLFMWKNTRINHNFIFDFSSNTALTHRDAFLMSASIMCTVVAALVINLFLKNAGVAYANALPGALLLLSTGVLFCPFDIFYRSTRYCFMRVMRNIIFSPFYKVLMADFFMADQLTSQIPLLRHMEFTACYFMAGSFRTHPYETCTSGQQYKHLAYVISFLPYFWRALQCLRRYLEEGHDINQLANAGKYVSAMVAAAVRFKYAATPTPFWVWMVIISSSGATIYQLYWDFVKDWGFLNPKSKNRWLRNELILKNKSIYYVSMMLNLALRLAWTESVMKIHIGKVESRLLDFSLASLEIIRRGHWNFYRLENEHLNNVGKFRAVKTVPLPFRELETD*