Gene Details:
- MSU gene ID: LOC_Os02g56120
- RAPdb gene ID: Os02g0805100
- Gene Symbol: OsIAA9
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Constitutive Expression of OsIAA9 Affects Starch Granules Accumulation and Root Gravitropic Response in Arabidopsis.
- Transgenic Arabidopsis plants expressing OsIAA9 have increased number of lateral roots, and reduced gravitropic response.
- Taken together, our study showed that OsIAA9 is a transcriptional repressor, and it regulates gravitropic response when expressed in Arabidopsis by regulating granules accumulation and distribution in root tips.
- Further analysis showed that OsIAA9 transgenic Arabidopsis plants accumulated fewer granules in their root tips and the distribution of granules was also affected.
- RT-PCR results showed that expression of OsIAA9 was induced by exogenously applied auxin, suggesting that OsIAA9 is an auxin response gene.
- When transfected into protoplasts isolated from rosette leaves of Arabidopsis, OsIAA9 repressed reporter gene expression, and the repression was partially released by exogenously IAA.
- By generating transgenic plants expressing GFP-OsIAA9 and examining florescence in the transgenic plants, we found that OsIAA9 is localized in the nucleus.
- Bioinformatic analysis showed that OsIAA9 has a repressor motif in Domain I, a degron in Domain II, and the conserved amino acid signatures for protein-protein interactions in Domain III and Domain IV.
Function-related keywords:
- gravitropic-response , root , starch , auxin , iaa , nucleus , R-protein , lateral-root , IAA , auxin-response
Literature:
- Constitutive Expression of OsIAA9 Affects Starch Granules Accumulation and Root Gravitropic Response in Arabidopsis . DOI: 10.3389/fpls.2015.01156 ; PMID: 26734051
- Comparative transcriptome profile analysis of rice varieties with different tolerance to zinc deficiency . DOI: 10.1111/plb.13227 ; PMID: 33296551
- Rice EIL1 interacts with OsIAAs to regulate auxin biosynthesis mediated by the tryptophan aminotransferase MHZ10/OsTAR2 during root ethylene responses . DOI: 10.1093/plcell/koac250 ; PMID: 35972379
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os02g56120.1
AAAGTCATCGCCATCATCGATCGATCGATCACAACTACTAGAAATCCAGCCAAGAAGATCGAGACCGGATACAGCTAGCAATCCATCCAAGAAGATCGAGAGAGCGGATACAGCTAGCCTAGCTACGCGAAGGATCAGATCATCGGCATGGAGCTGGAGCTTGGGCTGGCGCCACCGAATTCCGGTCATCTCGTCGTCGACGAGCTCAGCAGCAGCAGCAGCAGCGGCGGCGGCAGCGGCAGCGCGCCGGTGTCTGCCTCGTCGGCCGGGAAGAGGGGATTCAGGGAGGCGTTCCAGGAGACGCTGCTGCTCTTCGACGACGGCAGCTGCTGCAACACCAGCGACGACGATTGCAGGAGGAGGAAGAAGACGGTGGTGGGGTGGCCACCGGTGAGCTCGGCGCGGCGGGCGTGCGGCGGCGCCAACTACGTGAAGGTGAAGAAGGAAGGCGACGCCATCGGGAGGAAGGTTGATTTGGCCCTCCATTCCTCCTACGACGAGCTCGCTGCCACGCTCGCCCGCATGTTCCCCACCAACGACCACCAAGGCGAGAAGAAAATGGCCAATGATGATCATGGCGATGCCGCTGGACCTGTGGTCACCTACGAGGATGGTGATGGGGATTGGATGCTGGTTGGGGACGTGCCATGGGATGATTTTGCACGATCTGTGAAACGCCTGAAGATACTGGGTTAACTGAACTGAAGGAGATGCATGCGTGAGTTGATGGAGCACAGCTCGGTTTTGGTTGCCTGGCATCTGCGTGTGGGTGTGCACTGATGTATGTACATGTTTTCGTTTTCAGTCAGGATGTTAGGACGAGAGAAAGAGCTTTCTGTCAAAGACTATATACTGCTAGCTTATATATACTGTAGTTGGGCACATCTCATGTGTGCCCAGGGCTAGGTTTTGAGAATGTTTACAGAGTTCTGTGTTTTTACACCTTCTATATGTTTTGCAAACTGATCTTGTACTATAATACTACTTTTATTTATTTCTCTCTTTTTTTTCACACACCATATAA - >LOC_Os02g56120.2
AAAGTCATCGCCATCATCGATCGATCGATCACAACTACTAGAAATCCAGCCAAGAAGATCGAGACCGGATACAGCTAGCAATCCATCCAAGAAGATCGAGAGAGCGGATACAGCTAGCCTAGCTACGCGAAGGATCAGATCATCGGCATGGAGCTGGAGCTTGGGCTGGCGCCACCGAATTCCGGTCATCTCGTCGTCGACGAGCTCAGCAGCAGCAGCAGCAGCGGCGGCGGCAGCGGCAGCGCGCCGGTGTCTGCCTCGTCGGCCGGGAAGAGGGGATTCAGGGAGGCGTTCCAGGAGACGCTGCTGCTCTTCGACGACGGCAGCTGCTGCAACACCAGCGACGACGATTGCAGGAGGAGGAAGAAGACGGTGGTGGGGTGGCCACCGGTGAGCTCGGCGCGGCGGGCGTGCGGCGGCGCCAACTACGTGAAGGTGAAGAAGGAAGGCGACGCCATCGGGAGGAAGGTTGATTTGGCCCTCCATTCCTCCTACGACGAGCTCGCTGCCACGCTCGCCCGCATGTTCCCCACCAACGACCACCAAGGCGAGAAGAAAATGGCCAATGATGATCATGGCGATGCCGCTGGACCTGTGGTCACCTACGAGGATGGTGATGGGGATTGGATGCTGGTTGGGGACGTGCCATGGGATGATTTTGCACGATCTGTGAAACGCCTGAAGATACTGGGTTAACTGAACTGAAGGAGATGCATGCGTGAGTTGATGGAGCACAGCTCGGTTTTGGTTGCCTGGCATCTGCGTGTGGGTGTGCACTGATGTATGTACATGTTTTCGTTTTCAGTCAGGATGTTAGGACGAGAGAAAGAGCTTTCTGTCAAAGACTATATACTGCTAGCTTATATATACTGTAGTTGGGCACATCTCATGTGTGCCCAGGGCTAGGTTTTGAGAATGTTTACAGAGTTCTGTGTTTTTACACCTTCTATATGTTTTGCAAACTGATCTTGTACTATAATACTACTTTTATTTATTTCTCTCTTTTTTTTCACACACCATATAA - >LOC_Os02g56120.3
AAAGTCATCGCCATCATCGATCGATCGATCACAACTACTAGAAATCCAGCCAAGAAGATCGAGACCGGATACAGCTAGCAATCCATCCAAGAAGATCGAGAGAGCGGATACAGCTAGCCTAGCTACGCGAAGGATCAGATCATCGGCATGGAGCTGGAGCTTGGGCTGGCGCCACCGAATTCCGGTCATCTCGTCGTCGACGAGCTCAGCAGCAGCAGCAGCAGCGGCGGCGGCAGCGGCAGCGCGCCGGTGTCTGCCTCGTCGGCCGGGAAGAGGGGATTCAGGGAGGCGTTCCAGGAGACGCTGCTGCTCTTCGACGACGGCAGCTGCTGCAACACCAGCGACGACGATTGCAGGAGGAGGAAGAAGACGGTGGTGGGGTGGCCACCGGTGAGCTCGGCGCGGCGGGCGTGCGGCGGCGCCAACTACGTGAAGGTGAAGAAGGAAGGCGACGCCATCGGGAGGAAGGTTGATTTGGCCCTCCATTCCTCCTACGACGAGCTCGCTGCCACGCTCGCCCGCATGTTCCCCACCAACGACCACCAAGGCGAGAAGAAAATGGCCAATGATGATCATGGCGATGCCGCTGGACCTGTGGTCACCTACGAGGATGGTGATGGGGATTGGATGCTGGTTGGGGACGTGCCATGGGATGATTTTGCACGATCTGTGAAACGCCTGAAGATACTGGGTTAACTGAACTGAAGGAGATGCATGCGTGAGTTGATGGAGCACAGCTCGGTTTTGGTTGCCTGGCATCTGCGTGTGGGTGTGCACTGATGTATGTACATGTTTTCGTTTTCAGTCAGGATGTTAGGACGAGAGAAAGAGCTTTCTGTCAAAGACTATATACTGCTAGCTTATATATACTGTAGTTGGGCACATCTCATGTGTGCCCAGGGCTAGGTTTTGAGAATGTTTACAGAGTTCTGTGTTTTTACACCTTCTATATGTTTTGCAAACTGATCTTGTACTATAATACTACTTTTATTTATTTCTCTCTTTTTTTTCACACACCATATAA
CDS Sequence
- >LOC_Os02g56120.1
ATGGAGCTGGAGCTTGGGCTGGCGCCACCGAATTCCGGTCATCTCGTCGTCGACGAGCTCAGCAGCAGCAGCAGCAGCGGCGGCGGCAGCGGCAGCGCGCCGGTGTCTGCCTCGTCGGCCGGGAAGAGGGGATTCAGGGAGGCGTTCCAGGAGACGCTGCTGCTCTTCGACGACGGCAGCTGCTGCAACACCAGCGACGACGATTGCAGGAGGAGGAAGAAGACGGTGGTGGGGTGGCCACCGGTGAGCTCGGCGCGGCGGGCGTGCGGCGGCGCCAACTACGTGAAGGTGAAGAAGGAAGGCGACGCCATCGGGAGGAAGGTTGATTTGGCCCTCCATTCCTCCTACGACGAGCTCGCTGCCACGCTCGCCCGCATGTTCCCCACCAACGACCACCAAGGCGAGAAGAAAATGGCCAATGATGATCATGGCGATGCCGCTGGACCTGTGGTCACCTACGAGGATGGTGATGGGGATTGGATGCTGGTTGGGGACGTGCCATGGGATGATTTTGCACGATCTGTGAAACGCCTGAAGATACTGGGTTAA - >LOC_Os02g56120.2
ATGGAGCTGGAGCTTGGGCTGGCGCCACCGAATTCCGGTCATCTCGTCGTCGACGAGCTCAGCAGCAGCAGCAGCAGCGGCGGCGGCAGCGGCAGCGCGCCGGTGTCTGCCTCGTCGGCCGGGAAGAGGGGATTCAGGGAGGCGTTCCAGGAGACGCTGCTGCTCTTCGACGACGGCAGCTGCTGCAACACCAGCGACGACGATTGCAGGAGGAGGAAGAAGACGGTGGTGGGGTGGCCACCGGTGAGCTCGGCGCGGCGGGCGTGCGGCGGCGCCAACTACGTGAAGGTGAAGAAGGAAGGCGACGCCATCGGGAGGAAGGTTGATTTGGCCCTCCATTCCTCCTACGACGAGCTCGCTGCCACGCTCGCCCGCATGTTCCCCACCAACGACCACCAAGGCGAGAAGAAAATGGCCAATGATGATCATGGCGATGCCGCTGGACCTGTGGTCACCTACGAGGATGGTGATGGGGATTGGATGCTGGTTGGGGACGTGCCATGGGATGATTTTGCACGATCTGTGAAACGCCTGAAGATACTGGGTTAA - >LOC_Os02g56120.3
ATGGAGCTGGAGCTTGGGCTGGCGCCACCGAATTCCGGTCATCTCGTCGTCGACGAGCTCAGCAGCAGCAGCAGCAGCGGCGGCGGCAGCGGCAGCGCGCCGGTGTCTGCCTCGTCGGCCGGGAAGAGGGGATTCAGGGAGGCGTTCCAGGAGACGCTGCTGCTCTTCGACGACGGCAGCTGCTGCAACACCAGCGACGACGATTGCAGGAGGAGGAAGAAGACGGTGGTGGGGTGGCCACCGGTGAGCTCGGCGCGGCGGGCGTGCGGCGGCGCCAACTACGTGAAGGTGAAGAAGGAAGGCGACGCCATCGGGAGGAAGGTTGATTTGGCCCTCCATTCCTCCTACGACGAGCTCGCTGCCACGCTCGCCCGCATGTTCCCCACCAACGACCACCAAGGCGAGAAGAAAATGGCCAATGATGATCATGGCGATGCCGCTGGACCTGTGGTCACCTACGAGGATGGTGATGGGGATTGGATGCTGGTTGGGGACGTGCCATGGGATGATTTTGCACGATCTGTGAAACGCCTGAAGATACTGGGTTAA
Protein Sequence
- >LOC_Os02g56120.1
MELELGLAPPNSGHLVVDELSSSSSSGGGSGSAPVSASSAGKRGFREAFQETLLLFDDGSCCNTSDDDCRRRKKTVVGWPPVSSARRACGGANYVKVKKEGDAIGRKVDLALHSSYDELAATLARMFPTNDHQGEKKMANDDHGDAAGPVVTYEDGDGDWMLVGDVPWDDFARSVKRLKILG* - >LOC_Os02g56120.2
MELELGLAPPNSGHLVVDELSSSSSSGGGSGSAPVSASSAGKRGFREAFQETLLLFDDGSCCNTSDDDCRRRKKTVVGWPPVSSARRACGGANYVKVKKEGDAIGRKVDLALHSSYDELAATLARMFPTNDHQGEKKMANDDHGDAAGPVVTYEDGDGDWMLVGDVPWDDFARSVKRLKILG* - >LOC_Os02g56120.3
MELELGLAPPNSGHLVVDELSSSSSSGGGSGSAPVSASSAGKRGFREAFQETLLLFDDGSCCNTSDDDCRRRKKTVVGWPPVSSARRACGGANYVKVKKEGDAIGRKVDLALHSSYDELAATLARMFPTNDHQGEKKMANDDHGDAAGPVVTYEDGDGDWMLVGDVPWDDFARSVKRLKILG*