COP1;PPS

Gene Details

MSU gene ID: LOC_Os02g53140
RAPdb gene ID: Os02g0771100
Gene Symbol: COP1 PPS OsCOP1
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

In spite of its prolonged juvenile phase, the PPS-1 mutant flowers early, and this is associated with derepression of RAP1B expression in PPS-1 plants independently of the Hd1-Hd3a/RFT1 photoperiodic pathway.
Its ability to regulate the vegetative phase change and the time of flowering suggests that rice PPS acquired novel functions during the evolution of rice/monocots.
The COP1 ortholog PPS regulates the juvenile-adult and vegetative-reproductive phase changes in rice.
Although the early vegetative phase and flowering time of PPS-1 have been closely examined, the phenotypes in the late vegetative and reproductive phases are not yet well understood.
Role of rice PPS in late vegetative and reproductive growth.
Moreover, PPS-1 had a small inflorescence meristem and small panicles.
These phenotypes indicate that in PPS-1 the juvenile phase coexists with the late vegetative phase, resulting in small panicles.
Double mutant (d18-k PPS-1) showed the same phenotype as the PPS-1, indicating that PPS is upstream of GA biosynthetic genes.
In the ninth leaf blade of PPS-1, the relative length of the midrib was comparable to the sixth leaf blade of wild-type.
The PPS-1 mutant exhibits delayed expression of miR156 and miR172 and the suppression of GA biosynthetic genes, reducing the GA(3) content in this mutant.
The rice peter pan syndrome-1 (PPS-1) mutant shows a prolonged juvenile phase and early flowering.
OsCOP1 regulates embryo development and flavonoid biosynthesis in rice (Oryza sativa L.).
These results demonstrate that OsCOP1 is associated with embryo development and flavonoid biosynthesis in rice grains.
Novel mutations of OsCOP1 were identified to be responsible for yellowish pericarp and embryo lethal phenotype, which revealed that OsCOP1 plays a crucial role in flavonoid biosynthesis and embryogenesis in rice seed.
CRISPR/Cas9-targeted mutagenesis was used to knock out OsCOP1 by targeting its functional domains, and transgenic seed displayed the yel mutant phenotype.
Overexpression of OsCOP1 in a homozygous yel-hc mutant background restored pericarp color, and the aberrant flavonoid accumulation observed in yel-hc mutant was significantly reduced in the embryo and endosperm.
This study will facilitate a better understanding of the functional roles of OsCOP1 involved in early embryogenesis and flavonoid biosynthesis in rice seeds.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os02g53140.1
ACGCACATTTGCCCGCAGCGCCGCGGCGCGCGAGGCTACCCCGACCCCGACGTGGTCTCCTCCCACCCATACACCCTTCCGCTTCCACGCCCACACACAGAACCCAATTCCCCTCCCTCCTCCTCCACCCGCCCCTCCCTCCCACACACACCACACCACCACCACCACCACCCACCCCTCGTATGCAATTATTCACGCCCAGTCGCCGCCTCCACCGCCGCCGCCTGCTCGACTCACCACCGCAGGGCGGCCTCCTCCTGCCGCATGGGTGACTCGACGGTGGCCGGCGCGCTGGTGCCATCGGTGCCGAAGCAGGAGCAGGCGCCGTCGGGGGACGCGTCCACGGCGGCGTTGGCGGTGGCGGGGGAGGGGGAGGAGGATGCGGGGGCGCGCGCCTCCGCGGGGGGCAACGGGGAGGCCGCGGCCGACAGGGACCTCCTCTGCCCGATCTGCATGGCGGTCATCAAGGACGCCTTCCTCACCGCCTGCGGCCACAGCTTCTGCTACATGTGCATCGTCACGCATCTCAGCCACAAGAGCGACTGCCCCTGCTGCGGCAACTACCTCACCAAGGCGCAGCTCTACCCCAACTTCCTCCTCGACAAGGTCTTGAAGAAAATGTCAGCTCGCCAAATTGCGAAGACAGCATCACCGATAGACCAATTTCGATATGCACTGCAACAGGGAAACGATATGGCGGTTAAAGAACTAGATAGTCTTATGACTTTGATCGCGGAGAAGAAGCGGCATATGGAACAGCAAGAGTCAGAAACAAATATGCAAATATTGCTGGTCTTCTTGCATTGCCTCAGAAAGCAAAAGTTGGAAGAGCTGAATGAGATTCAAACTGACCTACAGTACATCAAAGAAGATATAAGTGCTGTGGAGAGACATAGGTTAGAATTATATCGAACAAAAGAAAGGTACTCAATGAAGCTCCGCATGCTTTTGGATGAACCTGCTGCATCAAAGATGTGGCCCTCACCTATGGATAAACCTAGTGGTCTCTTTCCTCCCAACTCTCGGGGACCACTTAGTACATCAAATCCAGGGGGTTTACAGAATAAGAAGCTTGACTTGAAAGGTCAAATTAGTCATCAAGGATTTCAAAGGAGAGATGTTCTCACTTGCTCGGATCCTCCTAGTGCCCCTATTCAATCAGGCAACGTTATTGCTCGGAAGAGGCGAGTTCAAGCTCAGTTTAACGAGCTTCAAGAATACTATCTTCAAAGACGGCGTACCGGAGCACAATCACGTAGGCTGGAGGAAAGAGACATAGTAACAATAAATAAAGAAGGTTATCATGCAGGACTTGAGGATTTCCAGTCTGTGCTAACAACATTCACACGATATAGTCGCTTGCGTGTAATTGCGGAGCTAAGACATGGAGATCTGTTTCACTCTGCAAATATCGTATCAAGTATCGAATTTGACCGTGATGATGAGCTATTTGCTACTGCTGGAGTCTCAAAGCGCATCAAAGTCTTCGAGTTTTCTACAGTTGTTAATGAACCATCAGATGTGCATTGTCCAGTTGTTGAAATGGCTACTAGATCTAAACTCAGCTGCCTTAGCTGGAACAAGTACTCAAAAAATGTTATAGCAAGCAGCGACTATGAGGGTATAGTAACTGTTTGGGATGTCCAAACCCGCCAGAGTGTGATGGAGTATGAAGAACATGAAAAGAGAGCATGGAGTGTTGATTTTTCTCGAACAGAACCCTCGATGCTAGTATCTGGGAGTGATGATTGCAAGGTCAAAGTGTGGTGCACAAAGCAAGAAGCAAGTGCCATCAATATTGATATGAAGGCCAATATTTGCTCTGTCAAATATAATCCTGGGTCGAGCCACTATGTTGCAGTGGGTTCTGCTGATCACCATATTCATTATTTTGATTTGCGAAATCCAAGTGCGCCTGTCCATGTTTTTGGTGGGCACAAGAAAGCTGTTTCTTATGTGAAGTTCCTGTCCACCAATGAGCTTGCGTCTGCATCAACTGATAGCACATTACGGTTATGGGATGTCAAAGAAAATTGCCCTGTAAGGACATTCAGAGGGCACAAGAATGAAAAGAACTTTGTTGGGCTGTCTGTAAATAACGAGTACATTGCCTGCGGGAGTGAAACGAATGAGGTTTTTGTTTACCACAAGGCTATCTCAAAACCTGCTGCCAACCACAGATTTGTATCATCTGATCTCGATGATGCAGATGATGATCCTGGCTCTTATTTTATTAGCGCAGTCTGCTGGAAGAGCGATAGCCCTACCATGTTAACTGCTAACAGTCAGGGCACCATTAAAGTTCTTGTACTTGCTCCTTGATGAAATCAGTGGTTTTCATGAGATCCCTAGATAGCTTGTATATTTGATGTATACAGTTGTTTCCTTTTCGTGCCATTATACCCCAAATGGGAGTGGAGGTATTACTGATCTCCAACATAGGGCGCAAAGTTTTGAAGGTAATCAGCTGACATAGGGTTTCGAGGGCTCGAAATGTGCATAGTCCAGAATTCTCATGTATAGGTTTAAAGCAGTCAAGTAATTGATTATACATATGTAACGTGAGAATTGAGAAATGAACATCAAATAAGCTTGTTTGGTTGCATATATTGGATTGGATTTTGGGAACTGGGAAGACAAGCTGTACTGGGCTGATTTCAGCAACACAAGCCAGTTTGTCACACATCTGCTACCTAACAGAAATAGTTAA

CDS Sequence

>LOC_Os02g53140.1
ATGGGTGACTCGACGGTGGCCGGCGCGCTGGTGCCATCGGTGCCGAAGCAGGAGCAGGCGCCGTCGGGGGACGCGTCCACGGCGGCGTTGGCGGTGGCGGGGGAGGGGGAGGAGGATGCGGGGGCGCGCGCCTCCGCGGGGGGCAACGGGGAGGCCGCGGCCGACAGGGACCTCCTCTGCCCGATCTGCATGGCGGTCATCAAGGACGCCTTCCTCACCGCCTGCGGCCACAGCTTCTGCTACATGTGCATCGTCACGCATCTCAGCCACAAGAGCGACTGCCCCTGCTGCGGCAACTACCTCACCAAGGCGCAGCTCTACCCCAACTTCCTCCTCGACAAGGTCTTGAAGAAAATGTCAGCTCGCCAAATTGCGAAGACAGCATCACCGATAGACCAATTTCGATATGCACTGCAACAGGGAAACGATATGGCGGTTAAAGAACTAGATAGTCTTATGACTTTGATCGCGGAGAAGAAGCGGCATATGGAACAGCAAGAGTCAGAAACAAATATGCAAATATTGCTGGTCTTCTTGCATTGCCTCAGAAAGCAAAAGTTGGAAGAGCTGAATGAGATTCAAACTGACCTACAGTACATCAAAGAAGATATAAGTGCTGTGGAGAGACATAGGTTAGAATTATATCGAACAAAAGAAAGGTACTCAATGAAGCTCCGCATGCTTTTGGATGAACCTGCTGCATCAAAGATGTGGCCCTCACCTATGGATAAACCTAGTGGTCTCTTTCCTCCCAACTCTCGGGGACCACTTAGTACATCAAATCCAGGGGGTTTACAGAATAAGAAGCTTGACTTGAAAGGTCAAATTAGTCATCAAGGATTTCAAAGGAGAGATGTTCTCACTTGCTCGGATCCTCCTAGTGCCCCTATTCAATCAGGCAACGTTATTGCTCGGAAGAGGCGAGTTCAAGCTCAGTTTAACGAGCTTCAAGAATACTATCTTCAAAGACGGCGTACCGGAGCACAATCACGTAGGCTGGAGGAAAGAGACATAGTAACAATAAATAAAGAAGGTTATCATGCAGGACTTGAGGATTTCCAGTCTGTGCTAACAACATTCACACGATATAGTCGCTTGCGTGTAATTGCGGAGCTAAGACATGGAGATCTGTTTCACTCTGCAAATATCGTATCAAGTATCGAATTTGACCGTGATGATGAGCTATTTGCTACTGCTGGAGTCTCAAAGCGCATCAAAGTCTTCGAGTTTTCTACAGTTGTTAATGAACCATCAGATGTGCATTGTCCAGTTGTTGAAATGGCTACTAGATCTAAACTCAGCTGCCTTAGCTGGAACAAGTACTCAAAAAATGTTATAGCAAGCAGCGACTATGAGGGTATAGTAACTGTTTGGGATGTCCAAACCCGCCAGAGTGTGATGGAGTATGAAGAACATGAAAAGAGAGCATGGAGTGTTGATTTTTCTCGAACAGAACCCTCGATGCTAGTATCTGGGAGTGATGATTGCAAGGTCAAAGTGTGGTGCACAAAGCAAGAAGCAAGTGCCATCAATATTGATATGAAGGCCAATATTTGCTCTGTCAAATATAATCCTGGGTCGAGCCACTATGTTGCAGTGGGTTCTGCTGATCACCATATTCATTATTTTGATTTGCGAAATCCAAGTGCGCCTGTCCATGTTTTTGGTGGGCACAAGAAAGCTGTTTCTTATGTGAAGTTCCTGTCCACCAATGAGCTTGCGTCTGCATCAACTGATAGCACATTACGGTTATGGGATGTCAAAGAAAATTGCCCTGTAAGGACATTCAGAGGGCACAAGAATGAAAAGAACTTTGTTGGGCTGTCTGTAAATAACGAGTACATTGCCTGCGGGAGTGAAACGAATGAGGTTTTTGTTTACCACAAGGCTATCTCAAAACCTGCTGCCAACCACAGATTTGTATCATCTGATCTCGATGATGCAGATGATGATCCTGGCTCTTATTTTATTAGCGCAGTCTGCTGGAAGAGCGATAGCCCTACCATGTTAACTGCTAACAGTCAGGGCACCATTAAAGTTCTTGTACTTGCTCCTTGA

Protein Sequence

>LOC_Os02g53140.1
MGDSTVAGALVPSVPKQEQAPSGDASTAALAVAGEGEEDAGARASAGGNGEAAADRDLLCPICMAVIKDAFLTACGHSFCYMCIVTHLSHKSDCPCCGNYLTKAQLYPNFLLDKVLKKMSARQIAKTASPIDQFRYALQQGNDMAVKELDSLMTLIAEKKRHMEQQESETNMQILLVFLHCLRKQKLEELNEIQTDLQYIKEDISAVERHRLELYRTKERYSMKLRMLLDEPAASKMWPSPMDKPSGLFPPNSRGPLSTSNPGGLQNKKLDLKGQISHQGFQRRDVLTCSDPPSAPIQSGNVIARKRRVQAQFNELQEYYLQRRRTGAQSRRLEERDIVTINKEGYHAGLEDFQSVLTTFTRYSRLRVIAELRHGDLFHSANIVSSIEFDRDDELFATAGVSKRIKVFEFSTVVNEPSDVHCPVVEMATRSKLSCLSWNKYSKNVIASSDYEGIVTVWDVQTRQSVMEYEEHEKRAWSVDFSRTEPSMLVSGSDDCKVKVWCTKQEASAINIDMKANICSVKYNPGSSHYVAVGSADHHIHYFDLRNPSAPVHVFGGHKKAVSYVKFLSTNELASASTDSTLRLWDVKENCPVRTFRGHKNEKNFVGLSVNNEYIACGSETNEVFVYHKAISKPAANHRFVSSDLDDADDDPGSYFISAVCWKSDSPTMLTANSQGTIKVLVLAP*