OsbZIP23

Gene Details

MSU gene ID: LOC_Os02g52780
RAPdb gene ID: Os02g0766700
Gene Symbol: OsbZIP23
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

Expression of OsbZIP23 is strongly induced by a wide spectrum of stresses, including drought, salt, abscisic acid (ABA), and polyethylene glycol treatments, while other stress-responsive genes of this family are slightly induced only by one or two of the stresses.
Transgenic rice overexpressing OsbZIP23 showed significantly improved tolerance to drought and high-salinity stresses and sensitivity to ABA.
On the other hand, a null mutant of this gene showed significantly decreased sensitivity to a high concentration of ABA and decreased tolerance to high-salinity and drought stress, and this phenotype can be complemented by transforming the OsbZIP23 back into the mutant.
We propose that OsbZIP23 is a major player of the bZIP family in rice for conferring ABA-dependent drought and salinity tolerance and has high potential usefulness in genetic improvement of stress tolerance.
Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice.
The stress-related genes activated by OsbZIP46CA1 are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23), further implying the value of OsbZIP46CA1 in genetic engineering of drought tolerance.
OsbZIP23 is a member of the basic leucine zipper (bZIP) transcription factor family in rice (Oryza sativa).
Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C.
It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidopsis thaliana) and rice, respectively.
Collectively, these results indicate that OsbZIP23 functions as a transcriptional regulator that can regulate the expression of a wide spectrum of stress-related genes in response to abiotic stresses through an ABA-dependent regulation pathway.
The single-copy OsbZIP23 gene is expressed at relatively higher level in leaf tissues of drought-tolerant genotypes, and its abundance is more in reproductive stage.
Enhanced Gene Expression Rather than Natural Polymorphism in Coding Sequence of the OsbZIP23 Determines Drought Tolerance and Yield Improvement in Rice Genotypes.
The overexpression of any of the two polymorphic forms (1083 bp and 1068 bp CDS) of OsbZIP23 improved drought tolerance and yield-related traits significantly by retaining higher content of cellular water, soluble sugar and proline; and exhibited decrease in membrane lipid peroxidation in comparison to RNAi lines and non-transgenic plants.
Taken together, the present study concludes that the enhanced gene expression rather than natural polymorphism in coding sequence of OsbZIP23 is accountable for improved drought tolerance and yield performance in rice genotypes.
The OE lines showed higher expression of target genes-OsRab16B, OsRab21 and OsLEA3-1 and increased ABA sensitivity; indicating that OsbZIP23 is a positive transcriptional-regulator of the ABA-signaling pathway.
The OsbZIP23 transcription factor has been characterized for its essential role in drought resistance in rice, but the mechanism is unknown.
Together, our results suggest that OsbZIP23 acts as a central regulator in ABA signaling and biosynthesis, and drought resistance in rice.
Next, we performed genome-wide identification of OsbZIP23 targets by immunoprecipitation sequencing (ChIP-seq) and RNA Sequencing (RNA-Seq) analyses in the OsbZIP23-overexpression, OsbZIP23 mutant, and wild-type rice under normal and drought stress conditions.
Among these targets, we found that OsbZIP23 could positively regulate OsPP2C49, and overexpression of OsPP2C49 in rice resulted in significantly decreased sensitivity of the ABA response and rapid dehydration.
Moreover, OsNCED4 (9-cis-epoxycarotenoid dioxygenase 4), a key gene in ABA biosynthesis, was also positively regulated by OsbZIP23.
OsbZIP23 directly regulates a large number of reported genes that function in stress response, hormone signaling, and developmental processes.
A homolog of SnRK2 protein kinase SAPK2 was found to interact with and phosphorylate OsbZIP23 for its transcriptional activation.
Synergistic regulation of drought-responsive genes by transcription factor OsbZIP23 and histone modification in rice.
Here, we report an interplay between histone H3K4me3 modification and transcription factor OsbZIP23 in the regulation of a dehydrin gene cluster under drought stress conditions in rice.
Conversely, the H3K4me3 modification and dehydrin gene expression levels were down-regulated in the OsbZIP23 mutant under drought stress conditions.
The transcription factor OsbZIP23 was demonstrated to regulate the UGT2 expression under stress conditions.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os02g52780.1
GGGTGTCACTTCGTCGCTCTCGAGGCCGGCGTTTTAAACGCCCCCCCCACACGGCTCCTCGCTGCCGACACCTGTCCGGTTTCCTCTCCTCGCCTCGCCGCGGCGCCCGCGTCCTCCTCACCAACGCCAGAGCTCCACATCCCACCTCTCCTCAGGTTCCCGTCTCTGATCCCTCGTTGCGTTACGGGGAAGCAGGCGTGGTGGTTGCTCCCCGTGCGGAGTTTTCGATTGGTTTGGTTGGGTGAGTTTGGTGCTGGGGTGTTGGGGATTGGTTGGTTGGAGTTTGGAGATGGATTTTCCGGGAGGGAGCGGGAGGCAGCAGCAGCTGCCGCCGATGACGCCGCTGCCGTTGGCGAGGCAGGGGTCGGTGTACTCGCTCACGTTCGACGAGTTCCAGAGCACGCTGGGCGGGGTCGGGAAGGACTTCGGGTCGATGAACATGGACGAGCTCCTCCGCAGCATCTGGACGGCCGAGGAGTCGCACGCCGTCGGCGCCGCCACGACGACGACGGCGACGACGGCGTCCGTGGCGGCGGCGGAGCACGCGGCGGTGGGGGCGCCGCCCGTTCAGAGGCAGGGGTCGCTGACCCTCCCCCGCACGCTCAGCCAGAAGACCGTCGACGAGGTCTGGCGCGACATGATGTGCTTCGGTGGCGGCGGCGCCTCCACCGCGCCGGCCGCCGCGGAGCCCCCGCCGCCGGCGCACCGGCAGCAGACGCTCGGGGAGATCACGCTGGAGGAGTTCCTCGTGCGGGCCGGCGTGGTGAGGGAGGACATGTCGGTCCCGCCCGTCCCGCCGGCGCCGACTCCTACGGCGGCTGCTGTACCTCCCCCGCCGCCGCCGCAGCAGCAGACGCCGATGTTGTTCGGTCAGAGCAATGTGTTCCCTCCGATGGTGCCTCCGCTCTCGCTGGGAAATGGGCTGGTCTCGGGAGCTGTCGGACACGGCGGTGGTGGTGCCGCGTCGTTGGTTTCGCCGGTGAGGCCGGTCTCGTCCAATGGCTTCGGCAAGATGGAAGGCGGGGACCTGTCGTCGCTGTCGCCATCGCCGGTGCCGTACGTTTTCAAAGGTGGGCTGAGGGGAAGGAAGGCACCGGGCATCGAGAAGGTTGTCGAGAGAAGACAGCGGCGGATGATCAAGAACAGGGAGTCTGCCGCGAGGTCGCGCCAGAGGAAACAGGCATATATGATGGAATTGGAAGCTGAGGTAGCAAAACTTAAGGAGCTGAACGATGAACTCCAGAAAAAGCAGGATGAAATGTTGGAGCAGCAAAAGAATGAGGTTCTAGAGAGAATGAGCCGACAAGTTGGACCGACAGCAAAGAGAATTTGCCTTCGGAGGACTCTGACGGGTCCATGGTGATCGAAGCTAAAGGCCTAGTTACCTGTAACTAGAACACAGTCCTTTCAATGTGAGCAGTGATCATAGTAGTTGTAGGATGGTGAAGCTGAAGACCTGGTTACCTGTAACTAGAACACAGTCCTTTCAAAGTGAGCAGTGATTACAGTAGTAGTAGGATGGTGAAGCTGCCAGTTACCTGTAACTAGAACACAGTCCTTTCAAAGTGAGTATAGTAGTAGGTTAGTTCAGAATCGCCAGATCGATCTGCCCTTGTAGGCCAACCATTAGTCCGTGGGCTTGTCTCTTGGGCGGACAGGCTGCCTGAGATATTCACTCGATAGGTTTTAGTTGTCTGAGTTATATCCAAGCATTGTATGTAACCTATTTAACCATGGTGCCTGCTACCTGTATTGTATTTTGCTGCTGTAGGAACCTATGTCGGTGTTACCATCTTGTTCTTAATTTGTGTACTTCTCTTGCCATTTTGGATAAGATGTATCTGAACTTATACATATGTACTTTTCCTTGCCATTTTGGACAAGATGTATCTGAATTTACATGAACCCATTTTGGACAACGTGTATCTGAATTTATATGAACCATGGCACTGGGCTGTGTGTGTATACTGT
>LOC_Os02g52780.2
GGGTGTCACTTCGTCGCTCTCGAGGCCGGCGTTTTAAACGCCCCCCCCACACGGCTCCTCGCTGCCGACACCTGTCCGGTTTCCTCTCCTCGCCTCGCCGCGGCGCCCGCGTCCTCCTCACCAACGCCAGAGCTCCACATCCCACCTCTCCTCAGGTTCCCGTCTCTGATCCCTCGTTGCGTTACGGGGAAGCAGGCGTGGTGGTTGCTCCCCGTGCGGAGTTTTCGATTGGTTTGGTTGGGTGAGTTTGGTGCTGGGGTGTTGGGGATTGGTTGGTTGGAGTTTGGAGATGGATTTTCCGGGAGGGAGCGGGAGGCAGCAGCAGCTGCCGCCGATGACGCCGCTGCCGTTGGCGAGGCAGGGGTCGGTGTACTCGCTCACGTTCGACGAGTTCCAGAGCACGCTGGGCGGGGTCGGGAAGGACTTCGGGTCGATGAACATGGACGAGCTCCTCCGCAGCATCTGGACGGCCGAGGAGTCGCACGCCGTCGGCGCCGCCACGACGACGACGGCGACGACGGCGTCCGTGGCGGCGGCGGAGCACGCGGCGGTGGGGGCGCCGCCCGTTCAGAGGCAGGGGTCGCTGACCCTCCCCCGCACGCTCAGCCAGAAGACCGTCGACGAGGTCTGGCGCGACATGATGTGCTTCGGTGGCGGCGGCGCCTCCACCGCGCCGGCCGCCGCGGAGCCCCCGCCGCCGGCGCACCGGCAGCAGACGCTCGGGGAGATCACGCTGGAGGAGTTCCTCGTGCGGGCCGGCGTGGTGAGGGAGGACATGTCGGTCCCGCCCGTCCCGCCGGCGCCGACTCCTACGGCGGCTGCTGTACCTCCCCCGCCGCCGCCGCAGCAGCAGACGCCGATGTTGTTCGGTCAGAGCAATGTGTTCCCTCCGATGGTGCCTCCGCTCTCGCTGGGAAATGGGCTGGTCTCGGGAGCTGTCGGACACGGCGGTGGTGGTGCCGCGTCGTTGGTTTCGCCGGTGAGGCCGGTCTCGTCCAATGGCTTCGGCAAGATGGAAGGCGGGGACCTGTCGTCGCTGTCGCCATCGCCGGTGCCGTACGTTTTCAAAGGTGGGCTGAGGGGAAGGAAGGCACCGGGCATCGAGAAGGTTGTCGAGAGAAGACAGCGGCGGATGATCAAGAACAGGGAGTCTGCCGCGAGGTCGCGCCAGAGGAAACAGGCATATATGATGGAATTGGAAGCTGAGGTAGCAAAACTTAAGGAGCTGAACGATGAACTCCAGAAAAAGCAGGATGAAATGTTGGAGCAGCAAAAGAATGAGGTTCTAGAGAGAATGAGCCGACAAGTTGGACCGACAGCAAAGAGAATTTGCCTTCGGAGGACTCTGACGGGTCCATGGTGATCGAAGCTAAAGGCCTAGTTACCTGTAACTAGAACACAGTCCTTTCAATGTGAGCAGTGATCATAGTAGTTGTAGGATGGTGAAGCTGAAGACCTGGTTACCTGTAACTAGAACACAGTCCTTTCAAAGTGAGCAGTGATTACAGTAGTAGTAGGATGGTGAAGCTGCCAGTTACCTGTAACTAGAACACAGTCCTTTCAAAGTGAGTATAGTAGTAGGTTAGTTCAGAATCGCCAGATCGATCTGCCCTTGTAGGCCAACCATTAGTCCGTGGGCTTGTCTCTTGGGCGGACAGGCTGCCTGAGATATTCACTCGATAGGTTTTAGTTGTCTGAGTTATATCCAAGCATTGTATGTAACCTATTTAACCATGGTGCCTGCTACCTGTATTGTATTTTGCTGCTGTAGGAACCTATGTCGGTGTTACCATCTTGTTCTTAATTTGTGTACTTCTCTTGCCATTTTGGATAAGATGTATCTGAACTTATACATATGTACTTTTCCTTGCCATTTTGGACAAGATGTATCTGAATTTACATGAACCCATTTTGGACAACGTGTATCTGAATTTATATGAACCATGGCACTGGGCTGTGTGTGTATACTGT

CDS Sequence

>LOC_Os02g52780.1
ATGGATTTTCCGGGAGGGAGCGGGAGGCAGCAGCAGCTGCCGCCGATGACGCCGCTGCCGTTGGCGAGGCAGGGGTCGGTGTACTCGCTCACGTTCGACGAGTTCCAGAGCACGCTGGGCGGGGTCGGGAAGGACTTCGGGTCGATGAACATGGACGAGCTCCTCCGCAGCATCTGGACGGCCGAGGAGTCGCACGCCGTCGGCGCCGCCACGACGACGACGGCGACGACGGCGTCCGTGGCGGCGGCGGAGCACGCGGCGGTGGGGGCGCCGCCCGTTCAGAGGCAGGGGTCGCTGACCCTCCCCCGCACGCTCAGCCAGAAGACCGTCGACGAGGTCTGGCGCGACATGATGTGCTTCGGTGGCGGCGGCGCCTCCACCGCGCCGGCCGCCGCGGAGCCCCCGCCGCCGGCGCACCGGCAGCAGACGCTCGGGGAGATCACGCTGGAGGAGTTCCTCGTGCGGGCCGGCGTGGTGAGGGAGGACATGTCGGTCCCGCCCGTCCCGCCGGCGCCGACTCCTACGGCGGCTGCTGTACCTCCCCCGCCGCCGCCGCAGCAGCAGACGCCGATGTTGTTCGGTCAGAGCAATGTGTTCCCTCCGATGGTGCCTCCGCTCTCGCTGGGAAATGGGCTGGTCTCGGGAGCTGTCGGACACGGCGGTGGTGGTGCCGCGTCGTTGGTTTCGCCGGTGAGGCCGGTCTCGTCCAATGGCTTCGGCAAGATGGAAGGCGGGGACCTGTCGTCGCTGTCGCCATCGCCGGTGCCGTACGTTTTCAAAGGTGGGCTGAGGGGAAGGAAGGCACCGGGCATCGAGAAGGTTGTCGAGAGAAGACAGCGGCGGATGATCAAGAACAGGGAGTCTGCCGCGAGGTCGCGCCAGAGGAAACAGGCATATATGATGGAATTGGAAGCTGAGGTAGCAAAACTTAAGGAGCTGAACGATGAACTCCAGAAAAAGCAGGATGAAATGTTGGAGCAGCAAAAGAATGAGGTTCTAGAGAGAATGAGCCGACAAGTTGGACCGACAGCAAAGAGAATTTGCCTTCGGAGGACTCTGACGGGTCCATGGTGA
>LOC_Os02g52780.2
ATGGATTTTCCGGGAGGGAGCGGGAGGCAGCAGCAGCTGCCGCCGATGACGCCGCTGCCGTTGGCGAGGCAGGGGTCGGTGTACTCGCTCACGTTCGACGAGTTCCAGAGCACGCTGGGCGGGGTCGGGAAGGACTTCGGGTCGATGAACATGGACGAGCTCCTCCGCAGCATCTGGACGGCCGAGGAGTCGCACGCCGTCGGCGCCGCCACGACGACGACGGCGACGACGGCGTCCGTGGCGGCGGCGGAGCACGCGGCGGTGGGGGCGCCGCCCGTTCAGAGGCAGGGGTCGCTGACCCTCCCCCGCACGCTCAGCCAGAAGACCGTCGACGAGGTCTGGCGCGACATGATGTGCTTCGGTGGCGGCGGCGCCTCCACCGCGCCGGCCGCCGCGGAGCCCCCGCCGCCGGCGCACCGGCAGCAGACGCTCGGGGAGATCACGCTGGAGGAGTTCCTCGTGCGGGCCGGCGTGGTGAGGGAGGACATGTCGGTCCCGCCCGTCCCGCCGGCGCCGACTCCTACGGCGGCTGCTGTACCTCCCCCGCCGCCGCCGCAGCAGCAGACGCCGATGTTGTTCGGTCAGAGCAATGTGTTCCCTCCGATGGTGCCTCCGCTCTCGCTGGGAAATGGGCTGGTCTCGGGAGCTGTCGGACACGGCGGTGGTGGTGCCGCGTCGTTGGTTTCGCCGGTGAGGCCGGTCTCGTCCAATGGCTTCGGCAAGATGGAAGGCGGGGACCTGTCGTCGCTGTCGCCATCGCCGGTGCCGTACGTTTTCAAAGGTGGGCTGAGGGGAAGGAAGGCACCGGGCATCGAGAAGGTTGTCGAGAGAAGACAGCGGCGGATGATCAAGAACAGGGAGTCTGCCGCGAGGTCGCGCCAGAGGAAACAGGCATATATGATGGAATTGGAAGCTGAGGTAGCAAAACTTAAGGAGCTGAACGATGAACTCCAGAAAAAGCAGGATGAAATGTTGGAGCAGCAAAAGAATGAGGTTCTAGAGAGAATGAGCCGACAAGTTGGACCGACAGCAAAGAGAATTTGCCTTCGGAGGACTCTGACGGGTCCATGGTGA

Protein Sequence

>LOC_Os02g52780.1
MDFPGGSGRQQQLPPMTPLPLARQGSVYSLTFDEFQSTLGGVGKDFGSMNMDELLRSIWTAEESHAVGAATTTTATTASVAAAEHAAVGAPPVQRQGSLTLPRTLSQKTVDEVWRDMMCFGGGGASTAPAAAEPPPPAHRQQTLGEITLEEFLVRAGVVREDMSVPPVPPAPTPTAAAVPPPPPPQQQTPMLFGQSNVFPPMVPPLSLGNGLVSGAVGHGGGGAASLVSPVRPVSSNGFGKMEGGDLSSLSPSPVPYVFKGGLRGRKAPGIEKVVERRQRRMIKNRESAARSRQRKQAYMMELEAEVAKLKELNDELQKKQDEMLEQQKNEVLERMSRQVGPTAKRICLRRTLTGPW*
>LOC_Os02g52780.2
MDFPGGSGRQQQLPPMTPLPLARQGSVYSLTFDEFQSTLGGVGKDFGSMNMDELLRSIWTAEESHAVGAATTTTATTASVAAAEHAAVGAPPVQRQGSLTLPRTLSQKTVDEVWRDMMCFGGGGASTAPAAAEPPPPAHRQQTLGEITLEEFLVRAGVVREDMSVPPVPPAPTPTAAAVPPPPPPQQQTPMLFGQSNVFPPMVPPLSLGNGLVSGAVGHGGGGAASLVSPVRPVSSNGFGKMEGGDLSSLSPSPVPYVFKGGLRGRKAPGIEKVVERRQRRMIKNRESAARSRQRKQAYMMELEAEVAKLKELNDELQKKQDEMLEQQKNEVLERMSRQVGPTAKRICLRRTLTGPW*