Gene Details:
- MSU gene ID: LOC_Os02g52710
- RAPdb gene ID: Os02g0765600
- Gene Symbol: AmyI-1 RAmy1A
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- The well-characterized secretory glycoprotein, rice (Oryza sativa) alpha-amylase isoform I-1 (AmyI-1), was localized within the plastids and proved to be involved in the degradation of starch granules in the organelles of rice cells.
- Functional dissections between GAMYB and Dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the RAmy1A gene in the rice aleurone.
- CARE and GARE were also found in the promoters of a rice alpha-amylase gene, RAmy1A, and a barley proteinase gene, EPB1, which are expressed in germinating seeds.
- The gibberellin-inducible rice α-amylase gene, RAmy1A, was demonstrated to be sugar repressed in rice embryos and functional dissection of the promoter of RAmy1A in relation of its sugar-modulated expression was performed.
- In this study, RAmy1A was expressed under the senescence-specific promoter of SAG12, which was designed to degrade starch in the leaf sheath and stem during grain filling.
- Senescence-Specific Expression of RAmy1A Accelerates Non-structural Carbohydrate Remobilization and Grain Filling in Rice ( Oryza sativa L.).
- RAmy1A mRNA successfully accumulated in the leaf, stem, and sheath of transgenic plants after anthesis.
Function-related keywords:
- starch , gibberellin , transcription-factor , seed , leaf , stem , grain , sheath , grain-filling
Literature:
- Functional dissection of a sugar-repressed alpha-amylase gene (RAmy1 A) promoter in rice embryos . DOI: 10.1016/s0014-5793(98)00067-2 ; PMID: 9506846
- Functional dissections between GAMYB and Dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the RAmy1A gene in the rice aleurone . DOI: 10.1104/pp.103.027334 ; PMID: 14500792
- Two cis-acting elements necessary and sufficient for gibberellin-upregulated proteinase expression in rice seeds . DOI: 10.1046/j.1365-313x.2003.01753.x ; PMID: 12787245
- SALT-RESPONSIVE ERF1 is a negative regulator of grain filling and gibberellin-mediated seedling establishment in rice . DOI: 10.1093/mp/sst131 ; PMID: 24046061
- Involvement of alpha-amylase I-1 in starch degradation in rice chloroplasts . DOI: 10.1093/pcp/pci091 ; PMID: 15821023
- The rice alpha-amylase glycoprotein is targeted from the Golgi apparatus through the secretory pathway to the plastids . DOI: 10.1105/tpc.109.068288 ; PMID: 19767453
- Senescence-Specific Expression of RAmy1A Accelerates Non-structural Carbohydrate Remobilization and Grain Filling in Rice (Oryza sativa L.) . DOI: 10.3389/fpls.2021.647574 ; PMID: 33986763
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os02g52710.1
TTCATCAATCATCCATCTCCGAAGTGTGTCTGCAGCATGCAGGTGCTGAACACCATGGTGAACAAACACTTCTTGTCCCTTTCGGTCCTCATCGTCCTCCTTGGCCTCTCCTCCAACTTGACAGCCGGGCAAGTCCTGTTTCAGGGATTCAACTGGGAGTCGTGGAAGGAGAATGGCGGGTGGTACAACTTCCTGATGGGCAAGGTGGACGACATCGCCGCAGCCGGCATCACCCACGTCTGGCTCCCTCCGCCGTCTCACTCTGTCGGCGAGCAAGGCTACATGCCTGGGCGGCTGTACGATCTGGACGCGTCTAAGTACGGCAACGAGGCGCAGCTCAAGTCGCTGATCGAGGCGTTCCATGGCAAGGGCGTCCAGGTGATCGCCGACATCGTCATCAACCACCGCACGGCGGAGCACAAGGACGGCCGCGGCATCTACTGCCTCTTCGAGGGCGGGACGCCCGACTCCCGCCTCGACTGGGGCCCGCACATGATCTGCCGCGACGACCCCTACGGCGATGGCACCGGCAACCCGGACACCGGCGCCGACTTCGCCGCCGCGCCGGACATCGACCACCTCAACAAGCGCGTCCAGCGGGAGCTCATTGGCTGGCTCGACTGGCTCAAGATGGACATCGGCTTCGACGCGTGGCGCCTCGACTTCGCCAAGGGCTACTCCGCCGACATGGCAAAGATCTACATCGACGCCACCGAGCCGAGCTTCGCCGTGGCCGAGATATGGACGTCCATGGCGAACGGCGGGGACGGCAAGCCGAACTACGACCAGAACGCGCACCGGCAGGAGCTGGTCAACTGGGTCGATCGTGTCGGCGGCGCCAACAGCAACGCCACGGCGTTCGACTTCACCACCAAGGGCATCCTCAACGTCGCCGTGGAGGGCGAGCTGTGGCGCCTCCGCGGCGAGGACGGCAAGGCGCCCGGCATGATCGGGTGGTGGCCGGCCAAGGCGACGACCTTCGTCGACAACCACGACACCGGCTCGACGCAGCACCTGTGGCCGTTCCCCTCCGACAAGGTCATGCAGGGCTACGCATACATCCTCACCCACCCCGGCAACCCATGCATCTTCTACGACCATTTCTTCGATTGGGGTCTCAAGGAGGAGATCGAGCGCCTGGTGTCAATCAGAAACCGGCAGGGGATCCACCCGGCGAGCGAGCTGCGCATCATGGAAGCTGACAGCGATCTCTACCTCGCGGAGATCGATGGCAAGGTGATCACAAAGATTGGACCAAGATACGACGTCGAACACCTCATCCCCGAAGGCTTCCAGGTCGTCGCGCACGGTGATGGCTACGCAATCTGGGAGAAAATCTGAGCGCACGATGACGAGACTCTCAGTTTAGCAGATTTAACCTGCGATTTTTACCCTGACCGGTATACGTATATACGTGCCGGCAACGAGCTGTATCCGATCCGAATTACGGATGCAATTGTCCACGAAGTACTTCCTCCGTAAATAAAGTGAGGATCAGGGACATACATTTGTATGGTTTTACGAATAATGCTATGCAATAAAATTTGCACTGCTTAATGCTTATGCATTTTTGCTTGGTTCGATTGTACTGGTGAATTATTGTTACTGTTCTTTTT
CDS Sequence
- >LOC_Os02g52710.1
ATGCAGGTGCTGAACACCATGGTGAACAAACACTTCTTGTCCCTTTCGGTCCTCATCGTCCTCCTTGGCCTCTCCTCCAACTTGACAGCCGGGCAAGTCCTGTTTCAGGGATTCAACTGGGAGTCGTGGAAGGAGAATGGCGGGTGGTACAACTTCCTGATGGGCAAGGTGGACGACATCGCCGCAGCCGGCATCACCCACGTCTGGCTCCCTCCGCCGTCTCACTCTGTCGGCGAGCAAGGCTACATGCCTGGGCGGCTGTACGATCTGGACGCGTCTAAGTACGGCAACGAGGCGCAGCTCAAGTCGCTGATCGAGGCGTTCCATGGCAAGGGCGTCCAGGTGATCGCCGACATCGTCATCAACCACCGCACGGCGGAGCACAAGGACGGCCGCGGCATCTACTGCCTCTTCGAGGGCGGGACGCCCGACTCCCGCCTCGACTGGGGCCCGCACATGATCTGCCGCGACGACCCCTACGGCGATGGCACCGGCAACCCGGACACCGGCGCCGACTTCGCCGCCGCGCCGGACATCGACCACCTCAACAAGCGCGTCCAGCGGGAGCTCATTGGCTGGCTCGACTGGCTCAAGATGGACATCGGCTTCGACGCGTGGCGCCTCGACTTCGCCAAGGGCTACTCCGCCGACATGGCAAAGATCTACATCGACGCCACCGAGCCGAGCTTCGCCGTGGCCGAGATATGGACGTCCATGGCGAACGGCGGGGACGGCAAGCCGAACTACGACCAGAACGCGCACCGGCAGGAGCTGGTCAACTGGGTCGATCGTGTCGGCGGCGCCAACAGCAACGCCACGGCGTTCGACTTCACCACCAAGGGCATCCTCAACGTCGCCGTGGAGGGCGAGCTGTGGCGCCTCCGCGGCGAGGACGGCAAGGCGCCCGGCATGATCGGGTGGTGGCCGGCCAAGGCGACGACCTTCGTCGACAACCACGACACCGGCTCGACGCAGCACCTGTGGCCGTTCCCCTCCGACAAGGTCATGCAGGGCTACGCATACATCCTCACCCACCCCGGCAACCCATGCATCTTCTACGACCATTTCTTCGATTGGGGTCTCAAGGAGGAGATCGAGCGCCTGGTGTCAATCAGAAACCGGCAGGGGATCCACCCGGCGAGCGAGCTGCGCATCATGGAAGCTGACAGCGATCTCTACCTCGCGGAGATCGATGGCAAGGTGATCACAAAGATTGGACCAAGATACGACGTCGAACACCTCATCCCCGAAGGCTTCCAGGTCGTCGCGCACGGTGATGGCTACGCAATCTGGGAGAAAATCTGA
Protein Sequence
- >LOC_Os02g52710.1
MQVLNTMVNKHFLSLSVLIVLLGLSSNLTAGQVLFQGFNWESWKENGGWYNFLMGKVDDIAAAGITHVWLPPPSHSVGEQGYMPGRLYDLDASKYGNEAQLKSLIEAFHGKGVQVIADIVINHRTAEHKDGRGIYCLFEGGTPDSRLDWGPHMICRDDPYGDGTGNPDTGADFAAAPDIDHLNKRVQRELIGWLDWLKMDIGFDAWRLDFAKGYSADMAKIYIDATEPSFAVAEIWTSMANGGDGKPNYDQNAHRQELVNWVDRVGGANSNATAFDFTTKGILNVAVEGELWRLRGEDGKAPGMIGWWPAKATTFVDNHDTGSTQHLWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDWGLKEEIERLVSIRNRQGIHPASELRIMEADSDLYLAEIDGKVITKIGPRYDVEHLIPEGFQVVAHGDGYAIWEKI*