Information report for OsCUL3a;OsSPL88
Gene Details
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Functional Descriptions
- The rice lesion mimic mutant OsCUL3a displays a significant increase in the accumulation of flg22- and chitin-induced reactive oxygen species, and in pathogenesis-related gene expression as well as resistance to Magnaporthe oryzae and Xanthomonas oryzae pv.
- OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice.
- Here, we show that rice OsCUL3a is important for the regulation of cell death and immunity.
- Strikingly, OsCUL3a interacts with and degrades OsNPR1, which acts as a positive regulator of cell death in rice.
- Our data demonstrate that OsCUL3a negatively regulates cell death and immunity by degrading OsNPR1 in rice.
- We cloned the OsCUL3a gene via a map-based strategy and found that the lesion mimic phenotype of OsCUL3a is associated with the early termination of OsCUL3a protein.
- Furthermore, the OsCUL3a osnpr1 double mutant does not exhibit the lesion mimic phenotype of the OsCUL3a mutant.
- In OsCUL3a (oscullin3a) mutants, OsCUL3a-associated molecular switches are responsible for disrupted cell metabolism that leads to increased total lipid content in rice grain, a late accumulation of H2O2 in leaves, enhanced Xoo disease resistance, and suppressed panicle and first internode growth.
- In OsCUL3a mutants, PRM confirmed up-regulated molecular switch proteins include lipoxygenases (CM-LOX1 and CM-LOX2), suggesting a novel connection between ferroptosis and rice lesion mimic formation.
- OsSPL88 Encodes a Cullin Protein that Regulates Rice Growth and Development.
Functional Keywords
- resistance , magnaporthe-oryzae , cell-death , immunity , lesion , lesion-mimic , reactive-oxygen-species , growth , panicle , disease , disease-resistance , xoo
Literature and News
- OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice . DOI: 10.1105/tpc.16.00650 ; PMID: 28100706
- OsCUL3a-Associated Molecular Switches Have Functions in Cell Metabolism, Cell Death, and Disease Resistance . DOI: 10.1021/acs.jafc.9b07426 ; PMID: 32320244
- OsSPL88 Encodes a Cullin Protein that Regulates Rice Growth and Development . DOI: 10.3389/fgene.2022.918973 ; PMID: 35899195
Gene Resources
- UniProt: Q6ZGT3
- EMBL: AP004114, AP005733, AP008208
- EnsemblPlants: Os02t0746000-01
- Gramene: Os02t0746000-01
- KEGG: dosa:Os02g0746000, osa:4330720
- Orthologous matrix: MFKDMTI
- InterPro: IPR001373, IPR016158, IPR016159
- PANTHER: PTHR11932
- SUPFAM: SSF46785, SSF74788, SSF75632
- PROSITE: PS50069
- Gene3D: 1.10.10.10, 1.20.1310.10, 3.30.230.130
- OrthoDB: Q6ZGT3
- SWISS-MODEL: Q6ZGT3
Sequences
cDNA Sequence
- >LOC_Os02g51180.1
GAGAGAGAAAAAAAAAAAGCAGGCGACGCCAGCACTGCAGGGAAGAAGAGGAACTGAAGCCTTTCTCTTCTGTCCGCCGCTGCGCCCTCCCCCTCTCTGTCCACGTTCCCCCACCCCACCTCGGCCTAAAACCCTAGCCGCCGGCGGAGCGGGACCTCACCGGCGGCGGCGATGAGCGGGGGCGGGCCGCCGAAGAAACGCAACTTCAAGATCGAGCTGTTCAAGCACCGCGTGGAGCTCGACCCCAAGTACGCGGAGCGGACATGGAAGGTCCTGGAGCACGCCATCCACGAGATCTACAACCACAACGCCAGTGGCCTCTCCTTCGAGGAGCTCTACAGGAGTGCCTACAACATGGTGCTCCACAAGTATGGTGAGAAGCTATATGATGGCCTGGAGAGAACTATGACATGGCGCTTGAAGGAAATATCAAAATCAATAGAGGCTGCACAGGGTGGTTTGTTTCTGGAGGAGCTGAATGCCAAGTGGATGGATCACAATAAGGCATTGCAGATGATCCGAGATATTCTAATGTACATGGATCGAACATATGTCCCGCAATCCCGTAGAACACCTGTTCATGAGCTTGGTTTGAATTTGTGGAGGGATCACATAATTCACTCTCCCATGATTCATAGTCGGCTGCTTGATACCCTTCTGGATCTTATTCACAGGGAGAGAATGGGTGAAATGATTAACAGAGGCCTGATGAGGAGCATAACGAAAATGTTAATGGATCTTGGTGCTGCTGTATATCAAGATGACTTTGAGAAGCCGTTTTTGGATGTTACAGCTAGCTTCTACAGTGGAGAGTCTCAGGAGTTCATTGAGTGCTGTGACTGTGGGAACTATCTTAAGAAGTCCGAGAGACGTCTCAATGAGGAAATGGAACGTGTCTCACACTACTTGGATTCTGGCACTGAGGCAAAGATAACTAGTGTGGTTGAGAAAGAAATGATTGCCAATCACATGCATAGATTGGTTCATATGGAAAACTCAGGCCTTGTAAATATGCTTGTAGATGACAAATATGACGACTTGGCTAGGATGTACAACTTATTCCGAAGGGTTTTTGATGGGCTATCGACAATCAGAGATGTGATGACTTCATACCTAAGGGAAACAGGGAAGCAGTTAGTGACAGATCCTGAGAGGTTGAAAGACCCAGTGGAATTTGTTCAGCGCTTGTTAAATGAGAAGGACAAGCATGATAAGATCATCAACGTTGCTTTTGGCAATGACAAAACTTTCCAGAATGCTCTAAATTCATCCTTTGAGTACTTCATCAACTTAAACAACAGGTCACCTGAATTCATATCGTTGTATGTTGATGATAAACTTCGGAAAGGATTGAAAGGAGCCACAGAAGAGGATGTGGAGGTTATTCTGGACAAAGTCATGATGCTGTTTCGGTACCTCCAGGAGAAGGATGTATTTGAGAAGTACTACAAGCAGCATTTGGCGAAAAGACTATTGTCTGGCAAAACTGTTTCTGATGATGCTGAGAGGAGTATGATAGTTAAACTCAAGACAGAATGTGGGTACCAGTTCACTTCCAAATTAGAGGGCATGTTCACTGACATGAAGACCTCTCAGGACACTATGATAGACTTTTATGCCAAGAAGTCTGAGGAACTTGGCGATGGCCCAACACTTGATGTCCACATTCTTACAACTGGTTCTTGGCCAACACAGCCCTGCCCTCCCTGCAACCTTCCAACTGAAATCCTTGCAATATGTGATAAGTTCCGGACATACTACCTTGGAACTCACAGTGGCCGGAGATTGACATGGCAAACAAACATGGGAACAGCTGACATAAAAGCCACATTTGGGAAAGGTCAGAAGCATGAACTAAATGTATCCACTTATCAGATGTGTGTTCTCATGCTGTTTAATTCTACCGATGGGTTAACCTACAAAGACATCGAACAAGATACTGCGATACCTGCCTCGGATCTAAAGAGATGCCTTCAATCTCTTGCTTGTGTCAAGGGGAAGAATGTTCTCCGCAAGGAACCCATGAGCAAAGATATATCGGAGGATGACACATTCTACTTCAACGACAAGTTCACAAGCAAGCTTGTTAAGGTCAAGATTGGGACAGTAGTGGCGCAAAAGGAATCTGAGCCAGAGAAACAAGAGACTCGTCAACGGGTTGAGGAGGACAGGAAACCTCAAATTGAGGCTGCTATCGTCAGGATTATGAAATCCAGGAGGGTCTTGGATCATAACAGCATTGTAGCTGAGGTTACCAAGCAATTGCAAGCCCGATTCATGCCGAATCCTGTTGTCATAAAGAAACGCATAGAATCTCTAATTGAGCGTGAATTCTTAGAGAGGGACAAAGCAGATAGGAAGTTATATCGCTATCTTGCATAGAAGGTCATTATGTGATTATTGATGAGCAAATGGCTTGTATTCATGTAAAACGGTTTCCATGACGTTTGAGTTCAGTTTTATTCGTTCCACTTTGTCCACTGAATCATAGCTACTTCTCGTTTCCCGTAGGCAAGCTGGTGTTCAGAGTTTTCCAGCTAATACTGATGTCATGTTACGAGTTGCTGCTATCTTAATATAATGGAGATGGATCGTCAAATTCACAGCC
CDS Sequence
- >LOC_Os02g51180.1
ATGAGCGGGGGCGGGCCGCCGAAGAAACGCAACTTCAAGATCGAGCTGTTCAAGCACCGCGTGGAGCTCGACCCCAAGTACGCGGAGCGGACATGGAAGGTCCTGGAGCACGCCATCCACGAGATCTACAACCACAACGCCAGTGGCCTCTCCTTCGAGGAGCTCTACAGGAGTGCCTACAACATGGTGCTCCACAAGTATGGTGAGAAGCTATATGATGGCCTGGAGAGAACTATGACATGGCGCTTGAAGGAAATATCAAAATCAATAGAGGCTGCACAGGGTGGTTTGTTTCTGGAGGAGCTGAATGCCAAGTGGATGGATCACAATAAGGCATTGCAGATGATCCGAGATATTCTAATGTACATGGATCGAACATATGTCCCGCAATCCCGTAGAACACCTGTTCATGAGCTTGGTTTGAATTTGTGGAGGGATCACATAATTCACTCTCCCATGATTCATAGTCGGCTGCTTGATACCCTTCTGGATCTTATTCACAGGGAGAGAATGGGTGAAATGATTAACAGAGGCCTGATGAGGAGCATAACGAAAATGTTAATGGATCTTGGTGCTGCTGTATATCAAGATGACTTTGAGAAGCCGTTTTTGGATGTTACAGCTAGCTTCTACAGTGGAGAGTCTCAGGAGTTCATTGAGTGCTGTGACTGTGGGAACTATCTTAAGAAGTCCGAGAGACGTCTCAATGAGGAAATGGAACGTGTCTCACACTACTTGGATTCTGGCACTGAGGCAAAGATAACTAGTGTGGTTGAGAAAGAAATGATTGCCAATCACATGCATAGATTGGTTCATATGGAAAACTCAGGCCTTGTAAATATGCTTGTAGATGACAAATATGACGACTTGGCTAGGATGTACAACTTATTCCGAAGGGTTTTTGATGGGCTATCGACAATCAGAGATGTGATGACTTCATACCTAAGGGAAACAGGGAAGCAGTTAGTGACAGATCCTGAGAGGTTGAAAGACCCAGTGGAATTTGTTCAGCGCTTGTTAAATGAGAAGGACAAGCATGATAAGATCATCAACGTTGCTTTTGGCAATGACAAAACTTTCCAGAATGCTCTAAATTCATCCTTTGAGTACTTCATCAACTTAAACAACAGGTCACCTGAATTCATATCGTTGTATGTTGATGATAAACTTCGGAAAGGATTGAAAGGAGCCACAGAAGAGGATGTGGAGGTTATTCTGGACAAAGTCATGATGCTGTTTCGGTACCTCCAGGAGAAGGATGTATTTGAGAAGTACTACAAGCAGCATTTGGCGAAAAGACTATTGTCTGGCAAAACTGTTTCTGATGATGCTGAGAGGAGTATGATAGTTAAACTCAAGACAGAATGTGGGTACCAGTTCACTTCCAAATTAGAGGGCATGTTCACTGACATGAAGACCTCTCAGGACACTATGATAGACTTTTATGCCAAGAAGTCTGAGGAACTTGGCGATGGCCCAACACTTGATGTCCACATTCTTACAACTGGTTCTTGGCCAACACAGCCCTGCCCTCCCTGCAACCTTCCAACTGAAATCCTTGCAATATGTGATAAGTTCCGGACATACTACCTTGGAACTCACAGTGGCCGGAGATTGACATGGCAAACAAACATGGGAACAGCTGACATAAAAGCCACATTTGGGAAAGGTCAGAAGCATGAACTAAATGTATCCACTTATCAGATGTGTGTTCTCATGCTGTTTAATTCTACCGATGGGTTAACCTACAAAGACATCGAACAAGATACTGCGATACCTGCCTCGGATCTAAAGAGATGCCTTCAATCTCTTGCTTGTGTCAAGGGGAAGAATGTTCTCCGCAAGGAACCCATGAGCAAAGATATATCGGAGGATGACACATTCTACTTCAACGACAAGTTCACAAGCAAGCTTGTTAAGGTCAAGATTGGGACAGTAGTGGCGCAAAAGGAATCTGAGCCAGAGAAACAAGAGACTCGTCAACGGGTTGAGGAGGACAGGAAACCTCAAATTGAGGCTGCTATCGTCAGGATTATGAAATCCAGGAGGGTCTTGGATCATAACAGCATTGTAGCTGAGGTTACCAAGCAATTGCAAGCCCGATTCATGCCGAATCCTGTTGTCATAAAGAAACGCATAGAATCTCTAATTGAGCGTGAATTCTTAGAGAGGGACAAAGCAGATAGGAAGTTATATCGCTATCTTGCATAG
Protein Sequence
- >LOC_Os02g51180.1
MSGGGPPKKRNFKIELFKHRVELDPKYAERTWKVLEHAIHEIYNHNASGLSFEELYRSAYNMVLHKYGEKLYDGLERTMTWRLKEISKSIEAAQGGLFLEELNAKWMDHNKALQMIRDILMYMDRTYVPQSRRTPVHELGLNLWRDHIIHSPMIHSRLLDTLLDLIHRERMGEMINRGLMRSITKMLMDLGAAVYQDDFEKPFLDVTASFYSGESQEFIECCDCGNYLKKSERRLNEEMERVSHYLDSGTEAKITSVVEKEMIANHMHRLVHMENSGLVNMLVDDKYDDLARMYNLFRRVFDGLSTIRDVMTSYLRETGKQLVTDPERLKDPVEFVQRLLNEKDKHDKIINVAFGNDKTFQNALNSSFEYFINLNNRSPEFISLYVDDKLRKGLKGATEEDVEVILDKVMMLFRYLQEKDVFEKYYKQHLAKRLLSGKTVSDDAERSMIVKLKTECGYQFTSKLEGMFTDMKTSQDTMIDFYAKKSEELGDGPTLDVHILTTGSWPTQPCPPCNLPTEILAICDKFRTYYLGTHSGRRLTWQTNMGTADIKATFGKGQKHELNVSTYQMCVLMLFNSTDGLTYKDIEQDTAIPASDLKRCLQSLACVKGKNVLRKEPMSKDISEDDTFYFNDKFTSKLVKVKIGTVVAQKESEPEKQETRQRVEEDRKPQIEAAIVRIMKSRRVLDHNSIVAEVTKQLQARFMPNPVVIKKRIESLIEREFLERDKADRKLYRYLA*