Gene Details:

Functional Descriptions:

  • The rice lesion mimic mutant OsCUL3a displays a significant increase in the accumulation of flg22- and chitin-induced reactive oxygen species, and in pathogenesis-related gene expression as well as resistance to Magnaporthe oryzae and Xanthomonas oryzae pv.
  • OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice.
  • Here, we show that rice OsCUL3a is important for the regulation of cell death and immunity.
  • Strikingly, OsCUL3a interacts with and degrades OsNPR1, which acts as a positive regulator of cell death in rice.
  • Our data demonstrate that OsCUL3a negatively regulates cell death and immunity by degrading OsNPR1 in rice.
  • We cloned the OsCUL3a gene via a map-based strategy and found that the lesion mimic phenotype of OsCUL3a is associated with the early termination of OsCUL3a protein.
  • Furthermore, the OsCUL3a osnpr1 double mutant does not exhibit the lesion mimic phenotype of the OsCUL3a mutant.
  • In OsCUL3a (oscullin3a) mutants, OsCUL3a-associated molecular switches are responsible for disrupted cell metabolism that leads to increased total lipid content in rice grain, a late accumulation of H2O2 in leaves, enhanced Xoo disease resistance, and suppressed panicle and first internode growth.
  • In OsCUL3a mutants, PRM confirmed up-regulated molecular switch proteins include lipoxygenases (CM-LOX1 and CM-LOX2), suggesting a novel connection between ferroptosis and rice lesion mimic formation.
  • OsSPL88 Encodes a Cullin Protein that Regulates Rice Growth and Development.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os02g51180.1
    GAGAGAGAAAAAAAAAAAGCAGGCGACGCCAGCACTGCAGGGAAGAAGAGGAACTGAAGCCTTTCTCTTCTGTCCGCCGCTGCGCCCTCCCCCTCTCTGTCCACGTTCCCCCACCCCACCTCGGCCTAAAACCCTAGCCGCCGGCGGAGCGGGACCTCACCGGCGGCGGCGATGAGCGGGGGCGGGCCGCCGAAGAAACGCAACTTCAAGATCGAGCTGTTCAAGCACCGCGTGGAGCTCGACCCCAAGTACGCGGAGCGGACATGGAAGGTCCTGGAGCACGCCATCCACGAGATCTACAACCACAACGCCAGTGGCCTCTCCTTCGAGGAGCTCTACAGGAGTGCCTACAACATGGTGCTCCACAAGTATGGTGAGAAGCTATATGATGGCCTGGAGAGAACTATGACATGGCGCTTGAAGGAAATATCAAAATCAATAGAGGCTGCACAGGGTGGTTTGTTTCTGGAGGAGCTGAATGCCAAGTGGATGGATCACAATAAGGCATTGCAGATGATCCGAGATATTCTAATGTACATGGATCGAACATATGTCCCGCAATCCCGTAGAACACCTGTTCATGAGCTTGGTTTGAATTTGTGGAGGGATCACATAATTCACTCTCCCATGATTCATAGTCGGCTGCTTGATACCCTTCTGGATCTTATTCACAGGGAGAGAATGGGTGAAATGATTAACAGAGGCCTGATGAGGAGCATAACGAAAATGTTAATGGATCTTGGTGCTGCTGTATATCAAGATGACTTTGAGAAGCCGTTTTTGGATGTTACAGCTAGCTTCTACAGTGGAGAGTCTCAGGAGTTCATTGAGTGCTGTGACTGTGGGAACTATCTTAAGAAGTCCGAGAGACGTCTCAATGAGGAAATGGAACGTGTCTCACACTACTTGGATTCTGGCACTGAGGCAAAGATAACTAGTGTGGTTGAGAAAGAAATGATTGCCAATCACATGCATAGATTGGTTCATATGGAAAACTCAGGCCTTGTAAATATGCTTGTAGATGACAAATATGACGACTTGGCTAGGATGTACAACTTATTCCGAAGGGTTTTTGATGGGCTATCGACAATCAGAGATGTGATGACTTCATACCTAAGGGAAACAGGGAAGCAGTTAGTGACAGATCCTGAGAGGTTGAAAGACCCAGTGGAATTTGTTCAGCGCTTGTTAAATGAGAAGGACAAGCATGATAAGATCATCAACGTTGCTTTTGGCAATGACAAAACTTTCCAGAATGCTCTAAATTCATCCTTTGAGTACTTCATCAACTTAAACAACAGGTCACCTGAATTCATATCGTTGTATGTTGATGATAAACTTCGGAAAGGATTGAAAGGAGCCACAGAAGAGGATGTGGAGGTTATTCTGGACAAAGTCATGATGCTGTTTCGGTACCTCCAGGAGAAGGATGTATTTGAGAAGTACTACAAGCAGCATTTGGCGAAAAGACTATTGTCTGGCAAAACTGTTTCTGATGATGCTGAGAGGAGTATGATAGTTAAACTCAAGACAGAATGTGGGTACCAGTTCACTTCCAAATTAGAGGGCATGTTCACTGACATGAAGACCTCTCAGGACACTATGATAGACTTTTATGCCAAGAAGTCTGAGGAACTTGGCGATGGCCCAACACTTGATGTCCACATTCTTACAACTGGTTCTTGGCCAACACAGCCCTGCCCTCCCTGCAACCTTCCAACTGAAATCCTTGCAATATGTGATAAGTTCCGGACATACTACCTTGGAACTCACAGTGGCCGGAGATTGACATGGCAAACAAACATGGGAACAGCTGACATAAAAGCCACATTTGGGAAAGGTCAGAAGCATGAACTAAATGTATCCACTTATCAGATGTGTGTTCTCATGCTGTTTAATTCTACCGATGGGTTAACCTACAAAGACATCGAACAAGATACTGCGATACCTGCCTCGGATCTAAAGAGATGCCTTCAATCTCTTGCTTGTGTCAAGGGGAAGAATGTTCTCCGCAAGGAACCCATGAGCAAAGATATATCGGAGGATGACACATTCTACTTCAACGACAAGTTCACAAGCAAGCTTGTTAAGGTCAAGATTGGGACAGTAGTGGCGCAAAAGGAATCTGAGCCAGAGAAACAAGAGACTCGTCAACGGGTTGAGGAGGACAGGAAACCTCAAATTGAGGCTGCTATCGTCAGGATTATGAAATCCAGGAGGGTCTTGGATCATAACAGCATTGTAGCTGAGGTTACCAAGCAATTGCAAGCCCGATTCATGCCGAATCCTGTTGTCATAAAGAAACGCATAGAATCTCTAATTGAGCGTGAATTCTTAGAGAGGGACAAAGCAGATAGGAAGTTATATCGCTATCTTGCATAGAAGGTCATTATGTGATTATTGATGAGCAAATGGCTTGTATTCATGTAAAACGGTTTCCATGACGTTTGAGTTCAGTTTTATTCGTTCCACTTTGTCCACTGAATCATAGCTACTTCTCGTTTCCCGTAGGCAAGCTGGTGTTCAGAGTTTTCCAGCTAATACTGATGTCATGTTACGAGTTGCTGCTATCTTAATATAATGGAGATGGATCGTCAAATTCACAGCC
CDS Sequence
  • >LOC_Os02g51180.1
    ATGAGCGGGGGCGGGCCGCCGAAGAAACGCAACTTCAAGATCGAGCTGTTCAAGCACCGCGTGGAGCTCGACCCCAAGTACGCGGAGCGGACATGGAAGGTCCTGGAGCACGCCATCCACGAGATCTACAACCACAACGCCAGTGGCCTCTCCTTCGAGGAGCTCTACAGGAGTGCCTACAACATGGTGCTCCACAAGTATGGTGAGAAGCTATATGATGGCCTGGAGAGAACTATGACATGGCGCTTGAAGGAAATATCAAAATCAATAGAGGCTGCACAGGGTGGTTTGTTTCTGGAGGAGCTGAATGCCAAGTGGATGGATCACAATAAGGCATTGCAGATGATCCGAGATATTCTAATGTACATGGATCGAACATATGTCCCGCAATCCCGTAGAACACCTGTTCATGAGCTTGGTTTGAATTTGTGGAGGGATCACATAATTCACTCTCCCATGATTCATAGTCGGCTGCTTGATACCCTTCTGGATCTTATTCACAGGGAGAGAATGGGTGAAATGATTAACAGAGGCCTGATGAGGAGCATAACGAAAATGTTAATGGATCTTGGTGCTGCTGTATATCAAGATGACTTTGAGAAGCCGTTTTTGGATGTTACAGCTAGCTTCTACAGTGGAGAGTCTCAGGAGTTCATTGAGTGCTGTGACTGTGGGAACTATCTTAAGAAGTCCGAGAGACGTCTCAATGAGGAAATGGAACGTGTCTCACACTACTTGGATTCTGGCACTGAGGCAAAGATAACTAGTGTGGTTGAGAAAGAAATGATTGCCAATCACATGCATAGATTGGTTCATATGGAAAACTCAGGCCTTGTAAATATGCTTGTAGATGACAAATATGACGACTTGGCTAGGATGTACAACTTATTCCGAAGGGTTTTTGATGGGCTATCGACAATCAGAGATGTGATGACTTCATACCTAAGGGAAACAGGGAAGCAGTTAGTGACAGATCCTGAGAGGTTGAAAGACCCAGTGGAATTTGTTCAGCGCTTGTTAAATGAGAAGGACAAGCATGATAAGATCATCAACGTTGCTTTTGGCAATGACAAAACTTTCCAGAATGCTCTAAATTCATCCTTTGAGTACTTCATCAACTTAAACAACAGGTCACCTGAATTCATATCGTTGTATGTTGATGATAAACTTCGGAAAGGATTGAAAGGAGCCACAGAAGAGGATGTGGAGGTTATTCTGGACAAAGTCATGATGCTGTTTCGGTACCTCCAGGAGAAGGATGTATTTGAGAAGTACTACAAGCAGCATTTGGCGAAAAGACTATTGTCTGGCAAAACTGTTTCTGATGATGCTGAGAGGAGTATGATAGTTAAACTCAAGACAGAATGTGGGTACCAGTTCACTTCCAAATTAGAGGGCATGTTCACTGACATGAAGACCTCTCAGGACACTATGATAGACTTTTATGCCAAGAAGTCTGAGGAACTTGGCGATGGCCCAACACTTGATGTCCACATTCTTACAACTGGTTCTTGGCCAACACAGCCCTGCCCTCCCTGCAACCTTCCAACTGAAATCCTTGCAATATGTGATAAGTTCCGGACATACTACCTTGGAACTCACAGTGGCCGGAGATTGACATGGCAAACAAACATGGGAACAGCTGACATAAAAGCCACATTTGGGAAAGGTCAGAAGCATGAACTAAATGTATCCACTTATCAGATGTGTGTTCTCATGCTGTTTAATTCTACCGATGGGTTAACCTACAAAGACATCGAACAAGATACTGCGATACCTGCCTCGGATCTAAAGAGATGCCTTCAATCTCTTGCTTGTGTCAAGGGGAAGAATGTTCTCCGCAAGGAACCCATGAGCAAAGATATATCGGAGGATGACACATTCTACTTCAACGACAAGTTCACAAGCAAGCTTGTTAAGGTCAAGATTGGGACAGTAGTGGCGCAAAAGGAATCTGAGCCAGAGAAACAAGAGACTCGTCAACGGGTTGAGGAGGACAGGAAACCTCAAATTGAGGCTGCTATCGTCAGGATTATGAAATCCAGGAGGGTCTTGGATCATAACAGCATTGTAGCTGAGGTTACCAAGCAATTGCAAGCCCGATTCATGCCGAATCCTGTTGTCATAAAGAAACGCATAGAATCTCTAATTGAGCGTGAATTCTTAGAGAGGGACAAAGCAGATAGGAAGTTATATCGCTATCTTGCATAG
Protein Sequence
  • >LOC_Os02g51180.1
    MSGGGPPKKRNFKIELFKHRVELDPKYAERTWKVLEHAIHEIYNHNASGLSFEELYRSAYNMVLHKYGEKLYDGLERTMTWRLKEISKSIEAAQGGLFLEELNAKWMDHNKALQMIRDILMYMDRTYVPQSRRTPVHELGLNLWRDHIIHSPMIHSRLLDTLLDLIHRERMGEMINRGLMRSITKMLMDLGAAVYQDDFEKPFLDVTASFYSGESQEFIECCDCGNYLKKSERRLNEEMERVSHYLDSGTEAKITSVVEKEMIANHMHRLVHMENSGLVNMLVDDKYDDLARMYNLFRRVFDGLSTIRDVMTSYLRETGKQLVTDPERLKDPVEFVQRLLNEKDKHDKIINVAFGNDKTFQNALNSSFEYFINLNNRSPEFISLYVDDKLRKGLKGATEEDVEVILDKVMMLFRYLQEKDVFEKYYKQHLAKRLLSGKTVSDDAERSMIVKLKTECGYQFTSKLEGMFTDMKTSQDTMIDFYAKKSEELGDGPTLDVHILTTGSWPTQPCPPCNLPTEILAICDKFRTYYLGTHSGRRLTWQTNMGTADIKATFGKGQKHELNVSTYQMCVLMLFNSTDGLTYKDIEQDTAIPASDLKRCLQSLACVKGKNVLRKEPMSKDISEDDTFYFNDKFTSKLVKVKIGTVVAQKESEPEKQETRQRVEEDRKPQIEAAIVRIMKSRRVLDHNSIVAEVTKQLQARFMPNPVVIKKRIESLIEREFLERDKADRKLYRYLA*