Gene Details:

Functional Descriptions:

  • Mutation of OsDRP2B disturbs the membrane trafficking that is essential for normal cellulose biosynthesis of the secondary cell wall, thereby leading to inferior mechanical properties in rice plants.
  • The BC3 mutation resulted in reductions of 28-36% in cellulose contents in culms, leaves, and roots, while other cell wall components remained unaffected.
  • From promoter-GUS analyses, it was suggested that BC3 expression is directly correlated with active secondary cell wall synthesis.
  • These results suggest that BC3 is tightly involved in the synthesis of cellulose and is essential for proper secondary cell wall construction.
  • Rice BRITTLE CULM 3 (BC3) encodes a classical dynamin OsDRP2B essential for proper secondary cell wall synthesis.
  • Here, we report the functional characterization of Brittle Culm3 (BC3), a gene encoding OsDRP2B.
  • Consistent with the expression of BC3 in mechanical tissues, the BC3 mutation reduces mechanical strength, which results from decreased cellulose content and altered secondary wall structure.
  • BC3 mutation and overexpression altered the abundance of cellulose synthase catalytic subunit 4 (OsCESA4) in the PM and in the endomembrane systems.
  • Through positional cloning, we have identified a gene responsible for the brittle culm phenotype in rice, brittle culm 3 (BC3).

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os02g50550.1
    CAACCACATCACATCACACCAGACGCCACCACGCCCCCCGAGATCTCTCCCACCCCCGACCAGCCTCGAGATCCCAGCGCCCATGTCGTCGTCGGCCATGGAGGCCATGGACGAGCTCGTCCAGCTCGCCGAGTCCATGCGCCAGGCCGCCTCCCTCCTGGCCGACGACGACCCCTCCGACGAGGCCAGCCCGCGCCGCCCCTCCACCTTCCTCAACGCCGTCGCGCTCGGCAACGTCGGCGCCGGCAAGTCGGCCGTGCTCAACAGCCTCATCGGCCACCCCGTGCTGCCGACGGGTGAGAACGGGGCGACGCGGGCGCCGATAGTGGTCGACCTGCAGAGGGATCCGGGGCTGAGCAGCAAATCCATCGTCCTGCAGATCGACAGCAAGTCGCAGCAGGTGTCGGCAAGTGCTCTCCGCCATTCGCTGCAGGACAGGCTGAGCAAGGGGGCGTCCGGTGGATCAGGGAAAGGCCGAAACGATGAGATTTACCTCAAGCTGCGGACAAGTACAGCTCCTCCAATAAAATTGGTCGATTTGCCTGGCATAGACCAACGAGTTATGGATGATTCAACTATCAGTGAATATGCTGGACATAATGATGCAATATTGATTGTTGTGATACCAGCAATGCAAGCTGCTGATGTTGCATCATCTCGAGCTCTCAGACTAGCGAAGGATATTGATCCAGATGGAACCAGAACAATAGGTGTTTTAAGTAAAATTGATCAAGCATCTGCAGATGCAAAAACTGTATCTTGTGTTCAGGCTATCTTGTCTAACAAGGGTGCACCACGAGCAGCAGCTGAAATTGAGTGGGTTGCTCTAATAGGGCAATCTGTCTCAATTGCATCGGCCCAAGCTGGATCAGTTGGTTCTGAGAACTCACTGGAAACTGCCTGGCAGGCTGAAGCGGAGACACTCAAATCCATCCTAACTGGAGCTCCTCGAAGTAAGCTAGGGAGAATTGCTCTCGTGGATACCATTGCTAAGCAGATACGTAAAAGGATGAAAATTCGGCTACCTAACCTTTTGTCCGGGCTTCAGGGGAAGTCTCAGATAGTGCAAAATGAACTTGCAAGGTTGGGAGAACAGATGGTATCAAGCGCTGAAGGAACCAGGGCTGTTGCTTTGGAGCTTTGCCGTGAATTCGAAGACAAGTTTCTTGCCCATGTTACTTCTGGTGAGGGGTCTGGTTGGAAAATTGTTGCAAGCTTTGAGGGGAAGTTTCCAGATAGGATCAAACAGCTTCCTTTGGACAGGCATTTTGATATTAACAATGTCAAAAGGATTGTCCTGGAAGCCGATGGTTATCAACCATATTTGATATCTCCAGAGAAAGGTTTAAAATCCTTAATAAAAGGAGTACTTGAAATGGCAAAGGAGCCATCACGGCTGTGTGTTGAAGAGGTACACCGTGTATTATTGGACATAGTAAATGCTTCTGCAAATGCTACACCAGGACTTGGAAGATATCCTCCATTCAAACGCGAGGTCGTTGAAATTGCAACCAATGCATTGGATGTCTTCAAAAGTGATGCAAAAAAGATGGTAGTTGCACTTGTTGATATGGAGCGTGCCTTTGTCCCACCACAACACTTCATCCGTTTAGTACAAAGAAGAATGGAAAGGCAACGTCGTGAGGATGAGGTGAAAAACAGGTCATCTAAGAAAGCCCAAGATGCAGAACAGTCCAACAAGGGCTCAGGCACAGGATCTGAGCAATCTGGTGGTGCCTTGAAATCGTTGAAAGAAAAATTCAGTCAGCAAGACAAAGATAAAGATGCAAAAGAGGGACCAAATTTGCAGGTTGCTGGGCCTGGTGGTGAAATAACTGCAGGCTACCTTTTGAAGAAAAGTGCAAAAAATAATGAATGGAGCAAAAGATGGTTTGTCCTAAATGAGAAGAGTGGAAAGCTTGGGTACACCAAGAAACAAGAGGAGAGACATTTTCGAGGTGTTATTGTCCTGGAGGAATGTAATCTGGAAGAGATAGAAGAGGAAGAGCTTTCCAAGAGTTTGAAGGATTCAAAGAAGGCCAATGGGGCAGAGAAAGGCCCAAGTCTTGTATTTAAGATTACTAACAGAGTTGCCTACAAAACTGTGCTGAAATCTCATAGTGCTGTTATACTGAAGGCCGAGACTATTGCTGACAAGGTTGAATGGATGAAGAAGATAAGAGGTGTCATTCAAAGCAAAGGGGGTTCTGTGAAGGGTTTAAATACTCCTGAGGGTGGTTCCATGAGGCAAAGCCATTCTGATGGGTCCCTTGATACGATGGCCCGGAAGCCTGCTGATCCTGAAGAAGAACTTCGGTGGATGTCTCAAGAAGTTCGTGGTTATGTAGAGGCTGTTTTGAACAGTTTGGCAGCAAATGTTCCAAAGGCAATCGTACTTTGCCAAGTGGAGAAAGCAAAGGAAGATATGCTAAATCAGCTCTACAGCAAAATAAGTGCTCAAACCAATGCAAAGATTGAAGAACTTCTCCAAGAGGACCACAATGCTAAACGTAGACGGGAGAAGTACCAAAAACAATCATCTCTATTGTCAAAGCTTACTCGTCAGCTTAGTATCCATGACAATAGAGCTTCTGTTGCATCGTATACCAATGATAGTTCTGGAGCTGAGAGTAGCCCACGGACCTCTGGCCAGTCAGGTGAGGACTGGAGGTCTGCGTTCGATTCTGCATCAAATGGCTCTGTTGATCGATCCAGTTCACACAATGAAACAAGGTCAAGAAGTGCTGACAGCCGTGGCAAACGCTATGAAAATGGGGACGTGAATGGTGGTAACTCTGGCAGCCGACGCACACCAAACCGTTTGCCACCAGCACCTCCAGGGCAAAAATATTAAGCATGTTGATCATCATCCGCCAACATCATATTTTCATGCTAGGTATGAAGTACAACGGAATTGATGCATCGAGAACCATTTGATTCAGGGGGATGGCATGCATGGGCTCGAAGAATAGAGCTGACGAGAGATTTGCATGAATGGTGTGTATCCAGAGGATCTCAGTTGTTCATAGAAATATACTCCTATAGTTTATCTACAGTTGTTTCACGATCATTTTGGCATATTGATCTGTCTGAGGCTCGCTCAGCCTGTGGGTGTGGGATTTTGTTACCACAGATCGGTTGATCTTGGTCTTGTACTCTGCTGTTGTAATTCTTCCATCAACTTGGCAATGTATTATTTCTTCCATTCTTTTTAACACGAGTCATTCTTTATTTGTGGGGCACACCGGATCACTAATATGTGTTTATACTGTGCAATTCTTTGATGACTGGCTTCCTTTGAAGAACCACGGGAAAAATGTACTACATGGTAACCCTTGTGTTCGTGTGACCACATAGTGCGCCATTTCTTACCAAAATGAAGGCCCATTGTCGAGAT
CDS Sequence
  • >LOC_Os02g50550.1
    ATGTCGTCGTCGGCCATGGAGGCCATGGACGAGCTCGTCCAGCTCGCCGAGTCCATGCGCCAGGCCGCCTCCCTCCTGGCCGACGACGACCCCTCCGACGAGGCCAGCCCGCGCCGCCCCTCCACCTTCCTCAACGCCGTCGCGCTCGGCAACGTCGGCGCCGGCAAGTCGGCCGTGCTCAACAGCCTCATCGGCCACCCCGTGCTGCCGACGGGTGAGAACGGGGCGACGCGGGCGCCGATAGTGGTCGACCTGCAGAGGGATCCGGGGCTGAGCAGCAAATCCATCGTCCTGCAGATCGACAGCAAGTCGCAGCAGGTGTCGGCAAGTGCTCTCCGCCATTCGCTGCAGGACAGGCTGAGCAAGGGGGCGTCCGGTGGATCAGGGAAAGGCCGAAACGATGAGATTTACCTCAAGCTGCGGACAAGTACAGCTCCTCCAATAAAATTGGTCGATTTGCCTGGCATAGACCAACGAGTTATGGATGATTCAACTATCAGTGAATATGCTGGACATAATGATGCAATATTGATTGTTGTGATACCAGCAATGCAAGCTGCTGATGTTGCATCATCTCGAGCTCTCAGACTAGCGAAGGATATTGATCCAGATGGAACCAGAACAATAGGTGTTTTAAGTAAAATTGATCAAGCATCTGCAGATGCAAAAACTGTATCTTGTGTTCAGGCTATCTTGTCTAACAAGGGTGCACCACGAGCAGCAGCTGAAATTGAGTGGGTTGCTCTAATAGGGCAATCTGTCTCAATTGCATCGGCCCAAGCTGGATCAGTTGGTTCTGAGAACTCACTGGAAACTGCCTGGCAGGCTGAAGCGGAGACACTCAAATCCATCCTAACTGGAGCTCCTCGAAGTAAGCTAGGGAGAATTGCTCTCGTGGATACCATTGCTAAGCAGATACGTAAAAGGATGAAAATTCGGCTACCTAACCTTTTGTCCGGGCTTCAGGGGAAGTCTCAGATAGTGCAAAATGAACTTGCAAGGTTGGGAGAACAGATGGTATCAAGCGCTGAAGGAACCAGGGCTGTTGCTTTGGAGCTTTGCCGTGAATTCGAAGACAAGTTTCTTGCCCATGTTACTTCTGGTGAGGGGTCTGGTTGGAAAATTGTTGCAAGCTTTGAGGGGAAGTTTCCAGATAGGATCAAACAGCTTCCTTTGGACAGGCATTTTGATATTAACAATGTCAAAAGGATTGTCCTGGAAGCCGATGGTTATCAACCATATTTGATATCTCCAGAGAAAGGTTTAAAATCCTTAATAAAAGGAGTACTTGAAATGGCAAAGGAGCCATCACGGCTGTGTGTTGAAGAGGTACACCGTGTATTATTGGACATAGTAAATGCTTCTGCAAATGCTACACCAGGACTTGGAAGATATCCTCCATTCAAACGCGAGGTCGTTGAAATTGCAACCAATGCATTGGATGTCTTCAAAAGTGATGCAAAAAAGATGGTAGTTGCACTTGTTGATATGGAGCGTGCCTTTGTCCCACCACAACACTTCATCCGTTTAGTACAAAGAAGAATGGAAAGGCAACGTCGTGAGGATGAGGTGAAAAACAGGTCATCTAAGAAAGCCCAAGATGCAGAACAGTCCAACAAGGGCTCAGGCACAGGATCTGAGCAATCTGGTGGTGCCTTGAAATCGTTGAAAGAAAAATTCAGTCAGCAAGACAAAGATAAAGATGCAAAAGAGGGACCAAATTTGCAGGTTGCTGGGCCTGGTGGTGAAATAACTGCAGGCTACCTTTTGAAGAAAAGTGCAAAAAATAATGAATGGAGCAAAAGATGGTTTGTCCTAAATGAGAAGAGTGGAAAGCTTGGGTACACCAAGAAACAAGAGGAGAGACATTTTCGAGGTGTTATTGTCCTGGAGGAATGTAATCTGGAAGAGATAGAAGAGGAAGAGCTTTCCAAGAGTTTGAAGGATTCAAAGAAGGCCAATGGGGCAGAGAAAGGCCCAAGTCTTGTATTTAAGATTACTAACAGAGTTGCCTACAAAACTGTGCTGAAATCTCATAGTGCTGTTATACTGAAGGCCGAGACTATTGCTGACAAGGTTGAATGGATGAAGAAGATAAGAGGTGTCATTCAAAGCAAAGGGGGTTCTGTGAAGGGTTTAAATACTCCTGAGGGTGGTTCCATGAGGCAAAGCCATTCTGATGGGTCCCTTGATACGATGGCCCGGAAGCCTGCTGATCCTGAAGAAGAACTTCGGTGGATGTCTCAAGAAGTTCGTGGTTATGTAGAGGCTGTTTTGAACAGTTTGGCAGCAAATGTTCCAAAGGCAATCGTACTTTGCCAAGTGGAGAAAGCAAAGGAAGATATGCTAAATCAGCTCTACAGCAAAATAAGTGCTCAAACCAATGCAAAGATTGAAGAACTTCTCCAAGAGGACCACAATGCTAAACGTAGACGGGAGAAGTACCAAAAACAATCATCTCTATTGTCAAAGCTTACTCGTCAGCTTAGTATCCATGACAATAGAGCTTCTGTTGCATCGTATACCAATGATAGTTCTGGAGCTGAGAGTAGCCCACGGACCTCTGGCCAGTCAGGTGAGGACTGGAGGTCTGCGTTCGATTCTGCATCAAATGGCTCTGTTGATCGATCCAGTTCACACAATGAAACAAGGTCAAGAAGTGCTGACAGCCGTGGCAAACGCTATGAAAATGGGGACGTGAATGGTGGTAACTCTGGCAGCCGACGCACACCAAACCGTTTGCCACCAGCACCTCCAGGGCAAAAATATTAA
Protein Sequence
  • >LOC_Os02g50550.1
    MSSSAMEAMDELVQLAESMRQAASLLADDDPSDEASPRRPSTFLNAVALGNVGAGKSAVLNSLIGHPVLPTGENGATRAPIVVDLQRDPGLSSKSIVLQIDSKSQQVSASALRHSLQDRLSKGASGGSGKGRNDEIYLKLRTSTAPPIKLVDLPGIDQRVMDDSTISEYAGHNDAILIVVIPAMQAADVASSRALRLAKDIDPDGTRTIGVLSKIDQASADAKTVSCVQAILSNKGAPRAAAEIEWVALIGQSVSIASAQAGSVGSENSLETAWQAEAETLKSILTGAPRSKLGRIALVDTIAKQIRKRMKIRLPNLLSGLQGKSQIVQNELARLGEQMVSSAEGTRAVALELCREFEDKFLAHVTSGEGSGWKIVASFEGKFPDRIKQLPLDRHFDINNVKRIVLEADGYQPYLISPEKGLKSLIKGVLEMAKEPSRLCVEEVHRVLLDIVNASANATPGLGRYPPFKREVVEIATNALDVFKSDAKKMVVALVDMERAFVPPQHFIRLVQRRMERQRREDEVKNRSSKKAQDAEQSNKGSGTGSEQSGGALKSLKEKFSQQDKDKDAKEGPNLQVAGPGGEITAGYLLKKSAKNNEWSKRWFVLNEKSGKLGYTKKQEERHFRGVIVLEECNLEEIEEEELSKSLKDSKKANGAEKGPSLVFKITNRVAYKTVLKSHSAVILKAETIADKVEWMKKIRGVIQSKGGSVKGLNTPEGGSMRQSHSDGSLDTMARKPADPEEELRWMSQEVRGYVEAVLNSLAANVPKAIVLCQVEKAKEDMLNQLYSKISAQTNAKIEELLQEDHNAKRRREKYQKQSSLLSKLTRQLSIHDNRASVASYTNDSSGAESSPRTSGQSGEDWRSAFDSASNGSVDRSSSHNETRSRSADSRGKRYENGDVNGGNSGSRRTPNRLPPAPPGQKY*