Information report for OsNCED1;OsCCD4a
Gene Details
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Functional Descriptions
- Collectively, the blocking of OsCCD4a activity might have potential as a practical tool for enhancing the carotenoid level of the carotenoid-accumulating seed endosperms as well as leaves of rice plants.
- Overexpression of OsNCED1 improved the HS tolerance of rice at the heading and flowering stage.
- These results suggested that OsNCED1 overexpression can improve the heat tolerance of rice by enhancing the antioxidant capacity.
- Comparative Analysis of Heat-Tolerant and Heat-Susceptible Rice Highlights the Role of OsNCED1 Gene in Heat Stress Tolerance.
- Rice seedlings overexpressing OsNCED1 showed enhanced heat tolerance with more abundant ABA content, whereas the knockout mutant OsNCED1 accumulated less ABA and showed more sensitive to heat stress.
- Here, we found that OsNCED1 was a heat stress inducible gene.
- Together, our results indicate that OsNCED1 positively regulates heat tolerance of rice seedling by raising endogenous ABA contents, which leads to the improved antioxidant capacity and activated expression of heat and ABA related genes.
- Moreover, real time-quantitative PCR (RT-qPCR) analysis showed that overexpression of OsNCED1 significantly activated the expression of genes involved in antioxidant enzymes, ABA signaling pathway, heat response, and defense.
- Under heat stress, increased expression of OsNCED1 could reduce membrane damage and reactive oxygen species (ROS) level of plants, and elevate the activity of antioxidant enzymes.
Functional Keywords
- seed , tolerance , stress , stress-tolerance , flowering , heat-stress , Heat-Stress , heat-tolerance , seedlings , seedling , defense , ABA , reactive-oxygen-species
Literature and News
- Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L . DOI: 10.1007/s00425-013-1853-9 ; PMID: 23397192
- The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice . DOI: 10.1371/journal.pgen.1001098 ; PMID: 20838584
- Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8’-hydroxylase in rice . DOI: 10.1093/pcp/pcm003 ; PMID: 17205969
- Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis . DOI: 10.1093/pcp/pcp022 ; PMID: 19208695
- RNAi-mediated suppression of three carotenoid-cleavage dioxygenase genes, OsCCD1, 4a, and 4b, increases carotenoid content in rice . DOI: 10.1093/jxb/ery300 ; PMID: 30124964
- Developmental analysis of the early steps in strigolactone-mediated axillary bud dormancy in rice . DOI: 10.1111/tpj.14266 ; PMID: 30740793
- ζ-Carotene Isomerase Suppresses Tillering in Rice through the Coordinated Biosynthesis of Strigolactone and Abscisic Acid . DOI: 10.1016/j.molp.2020.10.001 ; PMID: 33038484
- Comparative Analysis of Heat-Tolerant and Heat-Susceptible Rice Highlights the Role of OsNCED1 Gene in Heat Stress Tolerance . DOI: 10.3390/plants11081062 ; PMID: 35448790
- 9- cis-epoxycarotenoid dioxygenase 1 confers heat stress tolerance in rice seedling plants . DOI: 10.3389/fpls.2022.1092630 ; PMID: 36605966
Gene Resources
- UniProt: Q6YVJ0
- EMBL: AB110203, AK099580, AP005825
- AlphaFoldDB: Q6YVJ0
- EnsemblPlants: Os02t0704000-01
- Gramene: Os02t0704000-01
- KEGG: dosa:Os02g0704000, osa:4330451
Sequences
cDNA Sequence
- >LOC_Os02g47510.1
CGGTCTAGCACGAATCTGAAAGGGACAGCCTCTCTTAGCAAGAGAGGCCTTTATAAATAGCCGCACGGTTAATCTCACAACTCAGTGCAGTAGTAGTAGCATGCAAAGGATTTGCCCTGCTCACTGCTCGGTCACTCACTCACTCACCATGAAGTCCATGAGGCTTTCCTACATCCCTCCTGCTGCTTCTGCTGCTCCACAGAGCCCCAGCTATGGCAGGAAGAAGAACGCCTCCGCCGCTCCGCCATCGGCTGCCGCCTCCACCACCGTTCTCACCTCCCCGCTGGTGACCACCACCCGCACTCCGAAGCAGACCGAGCAAGAGGACGAGCAGTTGGTAGCCAAGACCAAGACTACGAGAACTGTTATTGCTACGACGAATGGCAGGGCGGCGCCGAGCCAGTCTCGGCCTCGCCGCCGGCCTGCCCCCGCCGCCGCGGCGTCGGCCGCTTCGCTGCCGATGACGTTCTGCAACGCGCTGGAGGAGGTGATCAACACGTTCATCGACCCGCCGGCGCTTCGGCCGGCGGTGGACCCGCGGAACGTGCTGACCAGCAACTTCGTGCCCGTGGACGAGCTGCCGCCGACGCCCTGCCCCGTCGTGCGCGGCGCCATCCCGCGCTGCCTCGCCGGCGGCGCCTACATCCGCAACGGGCCCAACCCGCAGCACCTCCCGCGCGGGCCGCACCACCTGTTCGACGGCGACGGCATGCTGCACTCCCTCCTCCTCCCGTCGCCCGCGTCGTCCGGCGACGACCCCGTCCTGTGCTCGCGCTACGTGCAGACGTACAAGTACCTCGTGGAGCGCGACGCCGGCGCGCCCGTCCTGCCCAACGTCTTCTCCGGCTTCCACGGCGTGGCCGGGATGGCGCGCGGCGCCGTCGTGGCGGCCAGGGTCCTGACCGGGCAGATGAATCCGTTGGAGGGCGTCGGGCTCGCCAACACCAGCCTCGCCTACTTCGCCGGCCGCCTCTACGCGCTCGGCGAGTCCGACCTCCCCTACGCCGTGCGCGTCCACCCGGACACCGGCGAGGTGACCACGCACGGCAGGTGCGACTTCGGCGGCCGCCTCGTCATGGGCATGACCGCGCACCCCAAGAAGGACCCCGTCACCGGCGAGCTCTTCGCGTTCCGCTACGGCCCCGTGCCGCCGTTCGTGACGTACTTCCGGTTCGACCCGGCCGGCAACAAGGGCGCCGACGTGCCCATCTTCTCCGTGCAGCAGCCGTCGTTCCTGCACGACTTCGCCATCACCGAGCGGTACGCCATCTTCCCGGAGATCCAGATCGTGATGAAGCCCATGGACATGGTGGTGGGCGGCGGCTCGCCCGTGGGGTCGGACCCCGGCAAGGTGCCCCGCCTCGGCGTGATCCCGCGCTACGCCACCGACGAGTCGGAGATGCGGTGGTTCGAGGTGCCGGGCTTCAACATCATGCACTCGGTGAACGCGTGGGAGGAGGCCGGCGGCGAGGAGCTGGTGCTGGTGGCGCCCAACGTCCTCTCCATCGAGCACGCCCTGGAGCACATGGAGCTAGTGCACTCCTGCGTCGAGAAGGTGCGCATCAACCTCCGCACCGGCGTCGTCACGCGCACCCCGCTCGCCGCCGGGAACTTCGACTTCCCCGTGATCAACCCGGCTTTCCTCGGCCGCCGCAACAGGTACGGCTACTTCGGCGTCGGCGACCCCGCGCCCAAGATCGGCGGCGTGGCCAAGCTCGACTTCGACCGCGCCGGCGAGGGCGACTGCACCGTGGCGCAGCGCGACTTCGGGCCCGGGTGCTTCGCCGGCGAACCGTTCTTCGTGGCCGACGACGTCGAGGGCAACGGCAACGAGGATGACGGGTACTTGGTGTGCTACGTCCACGACGAGGCCACCGGCGAGAACCGGTTCGTGGTGATGGACGCGCGGTCGCCGGACCTGGAGATCGTCGCGGAGGTGCAGCTGCCCGGACGCGTCCCCTACGGCTTCCATGGCCTGTTCGTCACGCAGGCCGAGCTCCAGTCACAGCACCAATGATACAAACCAACGTAGCAATGTATAGAATTCACGTATACATTTTCCATACGAAATTACGAGTTACTGTGGAACACGTAGTATAAGTGCACACATACTTCGAGAAAATTATGTAGAGTACATATGAAATGAAAGAATGATTTTAACTACACGAGCACAGATGCAAAACATAGTAA
CDS Sequence
- >LOC_Os02g47510.1
ATGCAAAGGATTTGCCCTGCTCACTGCTCGGTCACTCACTCACTCACCATGAAGTCCATGAGGCTTTCCTACATCCCTCCTGCTGCTTCTGCTGCTCCACAGAGCCCCAGCTATGGCAGGAAGAAGAACGCCTCCGCCGCTCCGCCATCGGCTGCCGCCTCCACCACCGTTCTCACCTCCCCGCTGGTGACCACCACCCGCACTCCGAAGCAGACCGAGCAAGAGGACGAGCAGTTGGTAGCCAAGACCAAGACTACGAGAACTGTTATTGCTACGACGAATGGCAGGGCGGCGCCGAGCCAGTCTCGGCCTCGCCGCCGGCCTGCCCCCGCCGCCGCGGCGTCGGCCGCTTCGCTGCCGATGACGTTCTGCAACGCGCTGGAGGAGGTGATCAACACGTTCATCGACCCGCCGGCGCTTCGGCCGGCGGTGGACCCGCGGAACGTGCTGACCAGCAACTTCGTGCCCGTGGACGAGCTGCCGCCGACGCCCTGCCCCGTCGTGCGCGGCGCCATCCCGCGCTGCCTCGCCGGCGGCGCCTACATCCGCAACGGGCCCAACCCGCAGCACCTCCCGCGCGGGCCGCACCACCTGTTCGACGGCGACGGCATGCTGCACTCCCTCCTCCTCCCGTCGCCCGCGTCGTCCGGCGACGACCCCGTCCTGTGCTCGCGCTACGTGCAGACGTACAAGTACCTCGTGGAGCGCGACGCCGGCGCGCCCGTCCTGCCCAACGTCTTCTCCGGCTTCCACGGCGTGGCCGGGATGGCGCGCGGCGCCGTCGTGGCGGCCAGGGTCCTGACCGGGCAGATGAATCCGTTGGAGGGCGTCGGGCTCGCCAACACCAGCCTCGCCTACTTCGCCGGCCGCCTCTACGCGCTCGGCGAGTCCGACCTCCCCTACGCCGTGCGCGTCCACCCGGACACCGGCGAGGTGACCACGCACGGCAGGTGCGACTTCGGCGGCCGCCTCGTCATGGGCATGACCGCGCACCCCAAGAAGGACCCCGTCACCGGCGAGCTCTTCGCGTTCCGCTACGGCCCCGTGCCGCCGTTCGTGACGTACTTCCGGTTCGACCCGGCCGGCAACAAGGGCGCCGACGTGCCCATCTTCTCCGTGCAGCAGCCGTCGTTCCTGCACGACTTCGCCATCACCGAGCGGTACGCCATCTTCCCGGAGATCCAGATCGTGATGAAGCCCATGGACATGGTGGTGGGCGGCGGCTCGCCCGTGGGGTCGGACCCCGGCAAGGTGCCCCGCCTCGGCGTGATCCCGCGCTACGCCACCGACGAGTCGGAGATGCGGTGGTTCGAGGTGCCGGGCTTCAACATCATGCACTCGGTGAACGCGTGGGAGGAGGCCGGCGGCGAGGAGCTGGTGCTGGTGGCGCCCAACGTCCTCTCCATCGAGCACGCCCTGGAGCACATGGAGCTAGTGCACTCCTGCGTCGAGAAGGTGCGCATCAACCTCCGCACCGGCGTCGTCACGCGCACCCCGCTCGCCGCCGGGAACTTCGACTTCCCCGTGATCAACCCGGCTTTCCTCGGCCGCCGCAACAGGTACGGCTACTTCGGCGTCGGCGACCCCGCGCCCAAGATCGGCGGCGTGGCCAAGCTCGACTTCGACCGCGCCGGCGAGGGCGACTGCACCGTGGCGCAGCGCGACTTCGGGCCCGGGTGCTTCGCCGGCGAACCGTTCTTCGTGGCCGACGACGTCGAGGGCAACGGCAACGAGGATGACGGGTACTTGGTGTGCTACGTCCACGACGAGGCCACCGGCGAGAACCGGTTCGTGGTGATGGACGCGCGGTCGCCGGACCTGGAGATCGTCGCGGAGGTGCAGCTGCCCGGACGCGTCCCCTACGGCTTCCATGGCCTGTTCGTCACGCAGGCCGAGCTCCAGTCACAGCACCAATGA
Protein Sequence
- >LOC_Os02g47510.1
MQRICPAHCSVTHSLTMKSMRLSYIPPAASAAPQSPSYGRKKNASAAPPSAAASTTVLTSPLVTTTRTPKQTEQEDEQLVAKTKTTRTVIATTNGRAAPSQSRPRRRPAPAAAASAASLPMTFCNALEEVINTFIDPPALRPAVDPRNVLTSNFVPVDELPPTPCPVVRGAIPRCLAGGAYIRNGPNPQHLPRGPHHLFDGDGMLHSLLLPSPASSGDDPVLCSRYVQTYKYLVERDAGAPVLPNVFSGFHGVAGMARGAVVAARVLTGQMNPLEGVGLANTSLAYFAGRLYALGESDLPYAVRVHPDTGEVTTHGRCDFGGRLVMGMTAHPKKDPVTGELFAFRYGPVPPFVTYFRFDPAGNKGADVPIFSVQQPSFLHDFAITERYAIFPEIQIVMKPMDMVVGGGSPVGSDPGKVPRLGVIPRYATDESEMRWFEVPGFNIMHSVNAWEEAGGEELVLVAPNVLSIEHALEHMELVHSCVEKVRINLRTGVVTRTPLAAGNFDFPVINPAFLGRRNRYGYFGVGDPAPKIGGVAKLDFDRAGEGDCTVAQRDFGPGCFAGEPFFVADDVEGNGNEDDGYLVCYVHDEATGENRFVVMDARSPDLEIVAEVQLPGRVPYGFHGLFVTQAELQSQHQ*