Gene Details:

• MSU gene ID: LOC_Os02g43410
• RAPdb gene ID: Os02g0650300
• Gene Symbol: OsYSL15
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• These results strongly suggest that OsYSL15 is the dominant iron(III)-deoxymugineic acid transporter responsible for iron uptake from the rhizosphere and is also responsible for phloem transport of iron.
• Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings.
• Expression of OsYSL15 in root epidermis and stele was induced by iron deficiency and showed daily fluctuation.
• OsYSL15 restored a yeast mutant defective in iron uptake when supplied with iron(III)-deoxymugineic acid and transported iron(III)-deoxymugineic acid in Xenopus laevis oocytes.
• OsYSL15 promoter-beta-glucuronidase analysis revealed that OsYSL15 expression in roots was dominant in the epidermis/exodermis and phloem cells under conditions of iron deficiency and was detected only in phloem under iron sufficiency.
• OsYSL15 knockdown seedlings showed severe arrest in germination and early growth and were rescued by high iron supply.
• These results demonstrate that rice OsYSL15 plays a crucial role in iron homeostasis during the early stages of growth.
• Two insertional OsYSL15 mutants exhibited chlorotic phenotypes under Fe deficiency and had reduced Fe concentrations in their shoots, roots, and seeds.
• Overexpression of OsYSL15 increased the Fe concentration in leaves and seeds from transgenic plants.
• OsYSL15 was also expressed in flowers, developing seeds, and in the embryonic scutellar epithelial cells during seed germination.
• RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1.
• The OsYSL15 promoter fusion to beta-glucuronidase showed that it was expressed in all root tissues when Fe was limited.
• We have characterized OsYSL15, one of the rice (Oryza sativa) YS1-like (YSL) genes that are strongly induced by iron (Fe) deficiency.
• Disruption of OsYSL15 leads to iron inefficiency in rice plants.

Function-related keywords:

• transporter ,  iron ,  seed ,  flower ,  seedling ,  root ,  shoot ,  transcription-factor ,  homeostasis ,  seed-germination ,  growth ,  ethylene

Literature:

• Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa .  DOI:  10.1093/jxb/erq301 ;  PMID:  21112958
• Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings .  DOI:  10.1074/jbc.M806042200 ;  PMID:  19049971
• A receptor-like protein RMC is involved in regulation of iron acquisition in rice .  DOI:  10.1093/jxb/ert290 ;  PMID:  24014863
• The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes .  DOI:  10.1111/j.1365-313X.2009.04015.x ;  PMID:  19737364
• Disruption of OsYSL15 leads to iron inefficiency in rice plants .  DOI:  10.1104/pp.109.135418 ;  PMID:  19376836
• Evolutionary expansion and functional diversification of oligopeptide transporter gene family in rice .  DOI:  10.1186/1939-8433-5-12 ;  PMID:  27234238
• Comparative transcriptome profile analysis of rice varieties with different tolerance to zinc deficiency .  DOI:  10.1111/plb.13227 ;  PMID:  33296551

Related News:

Gene Resources:

• NCBI ID: AB190923
• UniProt accessions:

Sequences: