Gene Details:

• MSU gene ID: LOC_Os02g43010
• RAPdb gene ID: Os02g0644000
• Gene Symbol: OsVPE3 REP-2
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Northern blot analysis indicated that REP-2 mRNA was expressed in both maturing and germinating seeds.
• Incubation of the de-embryonated seeds in 10(-6) M gibberellic acid induced the production of large amounts of REP-1, whereas REP-2beta levels declined rapidly.
• Identification, cDNA cloning and possible roles of seed-specific rice asparaginyl endopeptidase, REP-2.
• Bcl-2 suppresses hydrogen peroxide-induced programmed cell death via OsVPE2 and OsVPE3, but not via OsVPE1 and OsVPE4, in rice.
• Moreover, we found that suppression of OsVPE3 caused decreased leaf width and guard cell length in rice.
• Suppression of OsVPE3 Enhances Salt Tolerance by Attenuating Vacuole Rupture during Programmed Cell Death and Affects Stomata Development in Rice.
• Therefore, we concluded that OsVPE3 plays a crucial role in vacuole-mediated PCD and in stomatal development in rice.
• Survival rate and chlorophyll retention analyses showed that suppression of OsVPE3 clearly enhanced salt stress tolerance in transgenic rice compared with wild type.
• Taken together, these results indicated that suppression of OsVPE3 enhances salt tolerance by attenuating vacuole rupture during PCD.
• Endogenous hydrogen peroxide (H2O2) is involved in regulating the gibberellic acid-induced programmed cell death (PCD) of the aleurone layers by cooperating with OsVPE3 during rice seed germination.
• Further experiments indicated that GA up-regulated the OsVPE3 transcript and VPE activity, and the effect was reversed by DPI.
• Interaction between endogenous H 2 O 2 and OsVPE3 in the GA-induced PCD of rice aleurone layers.
• Furthermore, Ac-YVAD-CMK significantly blocked H2O2 accumulation, and DPI + Ac-YVAD-CMK had a more significant inhibitory effect compared with DPI alone, resulting in the delayed PCD, suggesting that OsVPE3 regulates PCD by promoting H2O2 generation.
• Meanwhile, DPI significantly inhibited the OsVPE3 transcript and VPE activity, and in turn delayed PCD occurrence, suggesting that the H2O2 produced by the NOX pathway may regulate PCD by up-regulating the OsVPE3 transcript.
• Thus, the endogenous H2O2 produced by the NOX pathway mediates the GA-induced PCD of rice aleurone layers by interacting with OsVPE3.

Function-related keywords:

• seed ,  cell-death ,  leaf ,  development ,  salt ,  tolerance ,  salt-tolerance ,  salt-stress ,  stress ,  stomatal ,  stomata ,  PCD ,  stress-tolerance ,  seed-germination ,  ga ,  GA ,  programmed-cell-death

Literature:

• Bcl-2 suppresses hydrogen peroxide-induced programmed cell death via OsVPE2 and OsVPE3, but not via OsVPE1 and OsVPE4, in rice .  DOI:  10.1111/j.1742-4658.2011.08380.x ;  PMID:  21972902
• Identification, cDNA cloning and possible roles of seed-specific rice asparaginyl endopeptidase, REP-2 .  DOI:  10.1007/s00425-003-1024-5 ;  PMID:  12684786
• Suppression of OsVPE3 Enhances Salt Tolerance by Attenuating Vacuole Rupture during Programmed Cell Death and Affects Stomata Development in Rice .  DOI:  10.1186/s12284-016-0138-x ;  PMID:  27900724
• Interaction between endogenous H2O2 and OsVPE3 in the GA-induced PCD of rice aleurone layers .  DOI:  10.1007/s00299-021-02665-w ;  PMID:  33559721

Related News:

Gene Resources:

• NCBI ID: AB081465
• UniProt accessions:

Sequences: