Information report for OsVPE3;REP-2
Gene Details
|
|
Functional Descriptions
- Northern blot analysis indicated that REP-2 mRNA was expressed in both maturing and germinating seeds.
- Incubation of the de-embryonated seeds in 10(-6) M gibberellic acid induced the production of large amounts of REP-1, whereas REP-2beta levels declined rapidly.
- Identification, cDNA cloning and possible roles of seed-specific rice asparaginyl endopeptidase, REP-2.
- Bcl-2 suppresses hydrogen peroxide-induced programmed cell death via OsVPE2 and OsVPE3, but not via OsVPE1 and OsVPE4, in rice.
- Moreover, we found that suppression of OsVPE3 caused decreased leaf width and guard cell length in rice.
- Suppression of OsVPE3 Enhances Salt Tolerance by Attenuating Vacuole Rupture during Programmed Cell Death and Affects Stomata Development in Rice.
- Therefore, we concluded that OsVPE3 plays a crucial role in vacuole-mediated PCD and in stomatal development in rice.
- Survival rate and chlorophyll retention analyses showed that suppression of OsVPE3 clearly enhanced salt stress tolerance in transgenic rice compared with wild type.
- Taken together, these results indicated that suppression of OsVPE3 enhances salt tolerance by attenuating vacuole rupture during PCD.
- Endogenous hydrogen peroxide (H2O2) is involved in regulating the gibberellic acid-induced programmed cell death (PCD) of the aleurone layers by cooperating with OsVPE3 during rice seed germination.
- Further experiments indicated that GA up-regulated the OsVPE3 transcript and VPE activity, and the effect was reversed by DPI.
- Interaction between endogenous H 2 O 2 and OsVPE3 in the GA-induced PCD of rice aleurone layers.
- Furthermore, Ac-YVAD-CMK significantly blocked H2O2 accumulation, and DPI + Ac-YVAD-CMK had a more significant inhibitory effect compared with DPI alone, resulting in the delayed PCD, suggesting that OsVPE3 regulates PCD by promoting H2O2 generation.
- Meanwhile, DPI significantly inhibited the OsVPE3 transcript and VPE activity, and in turn delayed PCD occurrence, suggesting that the H2O2 produced by the NOX pathway may regulate PCD by up-regulating the OsVPE3 transcript.
- Thus, the endogenous H2O2 produced by the NOX pathway mediates the GA-induced PCD of rice aleurone layers by interacting with OsVPE3.
Functional Keywords
- seed , cell-death , leaf , development , salt , tolerance , salt-tolerance , salt-stress , stress , stomatal , stomata , PCD , stress-tolerance , seed-germination , ga , GA , programmed-cell-death
Literature and News
- Bcl-2 suppresses hydrogen peroxide-induced programmed cell death via OsVPE2 and OsVPE3, but not via OsVPE1 and OsVPE4, in rice . DOI: 10.1111/j.1742-4658.2011.08380.x ; PMID: 21972902
- Identification, cDNA cloning and possible roles of seed-specific rice asparaginyl endopeptidase, REP-2 . DOI: 10.1007/s00425-003-1024-5 ; PMID: 12684786
- Suppression of OsVPE3 Enhances Salt Tolerance by Attenuating Vacuole Rupture during Programmed Cell Death and Affects Stomata Development in Rice . DOI: 10.1186/s12284-016-0138-x ; PMID: 27900724
- Interaction between endogenous H2O2 and OsVPE3 in the GA-induced PCD of rice aleurone layers . DOI: 10.1007/s00299-021-02665-w ; PMID: 33559721
Gene Resources
- UniProt: Q8GS39
- EMBL: AB081464, AB081465, AP005291
- AlphaFoldDB: Q8GS39
- EnsemblPlants: Os02t0644000-02
- Gramene: Os02t0644000-02
- KEGG: dosa:Os02g0644000, osa:4330133
- Orthologous matrix: RGVIINH
- InterPro: IPR001096, IPR043577, IPR046427
- PANTHER: PTHR12000, PTHR12000:SF42
- Gene3D: 1.10.132.130, 3.40.50.1460
- OrthoDB: Q8GS39
- SWISS-MODEL: Q8GS39
- Conserved Domain Database: cd21115
- eggNOG: KOG1348
Sequences
cDNA Sequence
- >LOC_Os02g43010.1
GACTTGTTTTAGTTGGGCTGGGCCACGCACGCCGAGGCGGCGGGCGGCGGTTGCCGTGGCTGCAGAAGACGACGAAGCGGCGGCGGCGCCACCTGTCCACCTCACGCCCCACGCGTCCGCGCCGCAGGCTACCCTCCTTGCATGGCGGCATGGCGCGGCGCCTACTTCGGTGCTTCCCTTCAACCACCTCGGTGTCCCGTAGAGTCGTAGGCGTGGTATTGTCGCGCGCGGAGAAGAGGGGAGGGATATGGCGGCGCGGTGGTGCTTCGCGTTGCTCTTGGCGCTGTCGGCGGCCGCGGCGGGGGCCGGAGCCAAGCGGACGTGGGAGCCGGTGATTCGGATGCCGGGGGAGGTGGTGGAGGAGGAGGTGGCCACCGTGCCGCGCGGCAGCGAGGGCACCGAGGAGGAGGAGAAGGACGGCGTCGGGACGAGGTGGGCGGTGCTCGTCGCGGGCTCCTCCGGCTACGGTAACTACAGGCACCAGGCTGATGTATGCCATGCATACCAGATATTACGGAAGGGAGGGCTAAAGGAGGAGAACATTGTCGTGTTTATGTATGACGACATTGCCAATAATATACTTAACCCGAGACCAGGGGTTATTGTCAATCATCCACAGGGTGAAGATGTTTATGCAGGAGTTCCGAAGGATTACACTGGAGACGAAGTCACTGCAAAAAACTTTTACGCGGTTCTCTTGGGCAATAAAACTGCAGTTACTGGGGGTAGTAGGAAGGTCATAGATAGCAAACCAAATGACCACATATTTATCTTCTACTCTGATCATGGGGGTCCTGGAGTTCTTGGTATGCCCAATCTGCCATATCTTTATGCTGCTGACTTCATGAAGGTGTTACAAGAAAAACATGCCTCCAATACTTACGCTAAAATGGTTATATATGTAGAAGCGTGTGAAAGTGGCAGTATTTTCGAAGGTTTGATGCCAGAGGACCTTAATATTTATGTCACAACAGCGTCTAATGCAGAAGAGAGTAGTTGGGGGACGTACTGCCCAGGAATGGAACCATCGCCTCCTTCCGAGTACATCACCTGCTTAGGTGACCTATACAGCGTTTCTTGGATGGAAGACAGTGAGACTCACAATCTGAAGGAGGAATCAATTAAGAAGCAGTACGAAGTGGTTAAGAAGCGTACCTCTGACATGAACAGCTACGGTGCTGGCTCTCATGTTATGGAGTACGGGGACAGGACATTCAAGGATGACAAGCTTTACCTATACCAAGGTTTCGACCCTGCAAATGCCGAAGTTAAGAACAAGCTATCATGGGAAGGCCCAAAGGCTGCAGTCAACCAAAGAGATGCAGATCTTCTTTTCCTCTGGAGAAGGTATGAGCTGTTACATGACAAGTCCGAAGAGAAGCTGAAAGCTCTGAGGGAGATCAGTGACACTGTTATGCATAGGAAGCTTCTTGACAGCAGTGTTGATCTTGTCGGAAAACTTCTCTTTGGATTTGGAAATGGGCCTTCTGTGCTTCAAGCTGTTAGACCTTCTGGTCAGCCACTCGTTGATGATTGGGATTGTTTGAAGAGAATGGTACGGATCTTTGAGTCGCATTGCGGACCGCTCACTCAGTACGGTATGAAGCACATGAGGGCGTTTGCTAATATTTGCAACAACGGTATCTCTGGCGCTTCAATGAAGGAAGCAAGCATCGCTACTTGCAGCAGTCACAACTCGGGTAGATGGAGCTCGCTGGTGCAGGGGTACAGTGCTTGATCTGCCGCAACACGTGATATAGCCTCAAAACTTATGCCTTGTACTCGTACTTCCTCAAAACAATACCTTCAGGACTGCAAATAACCGTCCAAGGATGTACCGCTGTATGCTACTGTACCTCTATGTGTGCAATATAGATGCCCTGTATATACGAGTATATGAATGAATCGTGTGCTTTTACATGCCCGGGAA - >LOC_Os02g43010.2
GACTTGTTTTAGTTGGGCTGGGCCACGCACGCCGAGGCGGCGGGCGGCGGTTGCCGTGGCTGCAGAAGACGACGAAGCGGCGGCGGCGCCACCTGTCCACCTCACGCCCCACGCGTCCGCGCCGCAGGCTACCCTCCTTGCATGGCGGCATGGCGCGGCGCCTACTTCGGTGCTTCCCTTCAACCACCTCGGTGTCCCGTAGAGTCGTAGGCGTGGTATTGTCGCGCGCGGAGAAGAGGGGAGGGATATGGCGGCGCGGTGGTGCTTCGCGTTGCTCTTGGCGCTGTCGGCGGCCGCGGCGGGGGCCGGAGCCAAGCGGACGTGGGAGCCGGTGATTCGGATGCCGGGGGAGGTGGTGGAGGAGGAGGTGGCCACCGTGCCGCGCGGCAGCGAGGGCACCGAGGAGGAGGAGAAGGACGGCGTCGGGACGAGGTGGGCGGTGCTCGTCGCGGGCTCCTCCGGCTACGGTAACTACAGGCACCAGGCTGATGTATGCCATGCATACCAGATATTACGGAAGGGAGGGCTAAAGGAGGAGAACATTGTCGTGTTTATGTATGACGACATTGCCAATAATATACTTAACCCGAGACCAGGGGTTATTGTCAATCATCCACAGGGTGAAGATGTTTATGCAGGAGTTCCGAAGGATTACACTGGAGACGAAGTCACTGCAAAAAACTTTTACGCGGTTCTCTTGGGCAATAAAACTGCAGTTACTGGGGGTAGTAGGAAGGTCATAGATAGCAAACCAAATGACCACATATTTATCTTCTACTCTGATCATGGGGGTCCTGGAGTTCTTGGTATGCCCAATCTGCCATATCTTTATGCTGCTGACTTCATGAAGGTGTTACAAGAAAAACATGCCTCCAATACTTACGCTAAAATGGTTATATATGTAGAAGCGTGTGAAAGTGGCAGTATTTTCGAAGGTTTGATGCCAGAGGACCTTAATATTTATGTCACAACAGCGTCTAATGCAGAAGAGAGTAGTTGGGGGACGTACTGCCCAGGAATGGAACCATCGCCTCCTTCCGAGTACATCACCTGCTTAGGTGACCTATACAGCGTTTCTTGGATGGAAGACAGTGAGACTCACAATCTGAAGGAGGAATCAATTAAGAAGCAGTACGAAGTGGTTAAGAAGCGTACCTCTGACATGAACAGCTACGGTGCTGGCTCTCATGTTATGGAGTACGGGGACAGGACATTCAAGGATGACAAGCTTTACCTATACCAAGGTTTCGACCCTGCAAATGCCGAAGTTAAGAACAAGCTATCATGGGAAGGCCCAAAGGCTGCAGTCAACCAAAGAGATGCAGATCTTCTTTTCCTCTGGAGAAGGTATGAGCTGTTACATGACAAGTCCGAAGAGAAGCTGAAAGCTCTGAGGGAGATCAGTGACACTGTTATGCATAGGAAGCTTCTTGACAGCAGTGTTGATCTTGTCGGAAAACTTCTCTTTGGATTTGGAAATGGGCCTTCTGTGCTTCAAGCTGTTAGACCTTCTGGTCAGCCACTCGTTGATGATTGGGATTGTTTGAAGAGAATGGTACGGATCTTTGAGTCGCATTGCGGACCGCTCACTCAGTACGGTATGAAGCACATGAGGGCGTTTGCTAATATTTGCAACAACGGTATCTCTGGCGCTTCAATGAAGGAAGCAAGCATCGCTACTTGCAGCAGTCACAACTCGGGTAGATGGAGCTCGCTGGTGCAGGGGTACAGTGCTTGATCTGCCGCAACACGTGATATAGCCTCAAAACTTATGCCTTGTACTCGTACTTCCTCAAAACAATACCTTCAGGACTGCAAATAACCGTCCAAGGATGTACCGCTGTATGCTACTGTACCTCTATGTGTGCAATATAGATGCCCTGTATATACGAGTATATGAATGAATCGTGTGCTTTTACATGCCCGGGAA
CDS Sequence
- >LOC_Os02g43010.1
ATGGCGGCGCGGTGGTGCTTCGCGTTGCTCTTGGCGCTGTCGGCGGCCGCGGCGGGGGCCGGAGCCAAGCGGACGTGGGAGCCGGTGATTCGGATGCCGGGGGAGGTGGTGGAGGAGGAGGTGGCCACCGTGCCGCGCGGCAGCGAGGGCACCGAGGAGGAGGAGAAGGACGGCGTCGGGACGAGGTGGGCGGTGCTCGTCGCGGGCTCCTCCGGCTACGGTAACTACAGGCACCAGGCTGATGTATGCCATGCATACCAGATATTACGGAAGGGAGGGCTAAAGGAGGAGAACATTGTCGTGTTTATGTATGACGACATTGCCAATAATATACTTAACCCGAGACCAGGGGTTATTGTCAATCATCCACAGGGTGAAGATGTTTATGCAGGAGTTCCGAAGGATTACACTGGAGACGAAGTCACTGCAAAAAACTTTTACGCGGTTCTCTTGGGCAATAAAACTGCAGTTACTGGGGGTAGTAGGAAGGTCATAGATAGCAAACCAAATGACCACATATTTATCTTCTACTCTGATCATGGGGGTCCTGGAGTTCTTGGTATGCCCAATCTGCCATATCTTTATGCTGCTGACTTCATGAAGGTGTTACAAGAAAAACATGCCTCCAATACTTACGCTAAAATGGTTATATATGTAGAAGCGTGTGAAAGTGGCAGTATTTTCGAAGGTTTGATGCCAGAGGACCTTAATATTTATGTCACAACAGCGTCTAATGCAGAAGAGAGTAGTTGGGGGACGTACTGCCCAGGAATGGAACCATCGCCTCCTTCCGAGTACATCACCTGCTTAGGTGACCTATACAGCGTTTCTTGGATGGAAGACAGTGAGACTCACAATCTGAAGGAGGAATCAATTAAGAAGCAGTACGAAGTGGTTAAGAAGCGTACCTCTGACATGAACAGCTACGGTGCTGGCTCTCATGTTATGGAGTACGGGGACAGGACATTCAAGGATGACAAGCTTTACCTATACCAAGGTTTCGACCCTGCAAATGCCGAAGTTAAGAACAAGCTATCATGGGAAGGCCCAAAGGCTGCAGTCAACCAAAGAGATGCAGATCTTCTTTTCCTCTGGAGAAGGTATGAGCTGTTACATGACAAGTCCGAAGAGAAGCTGAAAGCTCTGAGGGAGATCAGTGACACTGTTATGCATAGGAAGCTTCTTGACAGCAGTGTTGATCTTGTCGGAAAACTTCTCTTTGGATTTGGAAATGGGCCTTCTGTGCTTCAAGCTGTTAGACCTTCTGGTCAGCCACTCGTTGATGATTGGGATTGTTTGAAGAGAATGGTACGGATCTTTGAGTCGCATTGCGGACCGCTCACTCAGTACGGTATGAAGCACATGAGGGCGTTTGCTAATATTTGCAACAACGGTATCTCTGGCGCTTCAATGAAGGAAGCAAGCATCGCTACTTGCAGCAGTCACAACTCGGGTAGATGGAGCTCGCTGGTGCAGGGGTACAGTGCTTGA - >LOC_Os02g43010.2
ATGGCGGCGCGGTGGTGCTTCGCGTTGCTCTTGGCGCTGTCGGCGGCCGCGGCGGGGGCCGGAGCCAAGCGGACGTGGGAGCCGGTGATTCGGATGCCGGGGGAGGTGGTGGAGGAGGAGGTGGCCACCGTGCCGCGCGGCAGCGAGGGCACCGAGGAGGAGGAGAAGGACGGCGTCGGGACGAGGTGGGCGGTGCTCGTCGCGGGCTCCTCCGGCTACGGTAACTACAGGCACCAGGCTGATGTATGCCATGCATACCAGATATTACGGAAGGGAGGGCTAAAGGAGGAGAACATTGTCGTGTTTATGTATGACGACATTGCCAATAATATACTTAACCCGAGACCAGGGGTTATTGTCAATCATCCACAGGGTGAAGATGTTTATGCAGGAGTTCCGAAGGATTACACTGGAGACGAAGTCACTGCAAAAAACTTTTACGCGGTTCTCTTGGGCAATAAAACTGCAGTTACTGGGGGTAGTAGGAAGGTCATAGATAGCAAACCAAATGACCACATATTTATCTTCTACTCTGATCATGGGGGTCCTGGAGTTCTTGGTATGCCCAATCTGCCATATCTTTATGCTGCTGACTTCATGAAGGTGTTACAAGAAAAACATGCCTCCAATACTTACGCTAAAATGGTTATATATGTAGAAGCGTGTGAAAGTGGCAGTATTTTCGAAGGTTTGATGCCAGAGGACCTTAATATTTATGTCACAACAGCGTCTAATGCAGAAGAGAGTAGTTGGGGGACGTACTGCCCAGGAATGGAACCATCGCCTCCTTCCGAGTACATCACCTGCTTAGGTGACCTATACAGCGTTTCTTGGATGGAAGACAGTGAGACTCACAATCTGAAGGAGGAATCAATTAAGAAGCAGTACGAAGTGGTTAAGAAGCGTACCTCTGACATGAACAGCTACGGTGCTGGCTCTCATGTTATGGAGTACGGGGACAGGACATTCAAGGATGACAAGCTTTACCTATACCAAGGTTTCGACCCTGCAAATGCCGAAGTTAAGAACAAGCTATCATGGGAAGGCCCAAAGGCTGCAGTCAACCAAAGAGATGCAGATCTTCTTTTCCTCTGGAGAAGGTATGAGCTGTTACATGACAAGTCCGAAGAGAAGCTGAAAGCTCTGAGGGAGATCAGTGACACTGTTATGCATAGGAAGCTTCTTGACAGCAGTGTTGATCTTGTCGGAAAACTTCTCTTTGGATTTGGAAATGGGCCTTCTGTGCTTCAAGCTGTTAGACCTTCTGGTCAGCCACTCGTTGATGATTGGGATTGTTTGAAGAGAATGGTACGGATCTTTGAGTCGCATTGCGGACCGCTCACTCAGTACGGTATGAAGCACATGAGGGCGTTTGCTAATATTTGCAACAACGGTATCTCTGGCGCTTCAATGAAGGAAGCAAGCATCGCTACTTGCAGCAGTCACAACTCGGGTAGATGGAGCTCGCTGGTGCAGGGGTACAGTGCTTGA
Protein Sequence
- >LOC_Os02g43010.1
MAARWCFALLLALSAAAAGAGAKRTWEPVIRMPGEVVEEEVATVPRGSEGTEEEEKDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVFMYDDIANNILNPRPGVIVNHPQGEDVYAGVPKDYTGDEVTAKNFYAVLLGNKTAVTGGSRKVIDSKPNDHIFIFYSDHGGPGVLGMPNLPYLYAADFMKVLQEKHASNTYAKMVIYVEACESGSIFEGLMPEDLNIYVTTASNAEESSWGTYCPGMEPSPPSEYITCLGDLYSVSWMEDSETHNLKEESIKKQYEVVKKRTSDMNSYGAGSHVMEYGDRTFKDDKLYLYQGFDPANAEVKNKLSWEGPKAAVNQRDADLLFLWRRYELLHDKSEEKLKALREISDTVMHRKLLDSSVDLVGKLLFGFGNGPSVLQAVRPSGQPLVDDWDCLKRMVRIFESHCGPLTQYGMKHMRAFANICNNGISGASMKEASIATCSSHNSGRWSSLVQGYSA* - >LOC_Os02g43010.2
MAARWCFALLLALSAAAAGAGAKRTWEPVIRMPGEVVEEEVATVPRGSEGTEEEEKDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVFMYDDIANNILNPRPGVIVNHPQGEDVYAGVPKDYTGDEVTAKNFYAVLLGNKTAVTGGSRKVIDSKPNDHIFIFYSDHGGPGVLGMPNLPYLYAADFMKVLQEKHASNTYAKMVIYVEACESGSIFEGLMPEDLNIYVTTASNAEESSWGTYCPGMEPSPPSEYITCLGDLYSVSWMEDSETHNLKEESIKKQYEVVKKRTSDMNSYGAGSHVMEYGDRTFKDDKLYLYQGFDPANAEVKNKLSWEGPKAAVNQRDADLLFLWRRYELLHDKSEEKLKALREISDTVMHRKLLDSSVDLVGKLLFGFGNGPSVLQAVRPSGQPLVDDWDCLKRMVRIFESHCGPLTQYGMKHMRAFANICNNGISGASMKEASIATCSSHNSGRWSSLVQGYSA*