Gene Details:

• MSU gene ID: LOC_Os02g41904
• RAPdb gene ID: Os02g0629800
• Gene Symbol: OsDEF7 CAL1 OsAFP1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• CAL1 is expressed preferentially in root exodermis and xylem parenchyma cells.
• We provide evidence that CAL1 acts by chelating Cd in the cytosol and facilitating Cd secretion to extracellular spaces, hence lowering cytosolic Cd concentration while driving long-distance Cd transport via xylem vessels.
• OsAFP1 exerted fungicidal activity against Candida albicans, the most common pathogenic fungus in humans, at 4<U+2009>M concentration, but it did not inhibit the growth of human pathogenic bacteria.
• Immunohistochemistry showed that the OsAFP1 target molecule was located in the cell wall.
• Further, PI uptake and apoptosis assays suggested that OsAFP1 exerts its antifungal activity by inducing apoptosis of target cells.
• Partial peptides from rice defensin OsAFP1 exhibited antifungal activity against the rice blast pathogen Pyricularia oryzae.
• In lipid-binding analyses performed using nitrocellulose membranes immobilized with various membrane lipid components, OsAFP1 was found to bind to phosphatidylinositols (PIPs) harboring phosphate groups, particularly PI(3)P.

Function-related keywords:

• xylem ,  root ,  xylem-parenchyma ,  growth ,  cell-wall ,  Pi ,  pi ,  Pi-uptake ,  blast ,  pathogen ,  phosphate

Literature:

• Gene expression analysis, subcellular localization, and in planta antimicrobial activity of rice (Oryza sativa L.) defensin 7 and 8 .  DOI:  10.1016/j.plaphy.2018.01.011 ;  PMID:  29414311
• A defensin-like protein drives cadmium efflux and allocation in rice .  DOI:  10.1038/s41467-018-03088-0 ;  PMID:  29440679
• Rice Defensin OsAFP1 is a New Drug Candidate against Human Pathogenic Fungi .  DOI:  10.1038/s41598-018-29715-w ;  PMID:  30061724
• Partial peptides from rice defensin OsAFP1 exhibited antifungal activity against the rice blast pathogen Pyricularia oryzae .  DOI:  10.1584/jpestics.D17-046 ;  PMID:  30363094
• Crystal structure of rice defensin OsAFP1 and molecular insight into lipid-binding .  DOI:  10.1016/j.jbiosc.2020.02.011 ;  PMID:  32192842
• Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment .  DOI:  10.1371/journal.pone.0243174 ;  PMID:  33301482

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: