Information report for OsCER1;OsGL1-4
Gene Details
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Functional Descriptions
- OsCER1 was highly expressed in the tapetum (stage 10) and bicellular pollen cells (stage 11).
- Downregulation of OsCER1 in rice led to sterility, and fewer amyloplasts within the mature pollen grains.
- Furthermore, significantly altered levels of expression of genes involved in the pollen development were exhibited in the OsCER1 knocked down plants.
- These results indicate that OsCER1 is critical for VLC alkanes biosynthesis, plastids differentiation, and pollen development.
- OsCER1 Plays a Pivotal Role in Very-Long-Chain Alkane Biosynthesis and Affects Plastid Development and Programmed Cell Death of Tapetum in Rice (Oryza sativa L.).
- In addition, the downregulation of OsCER1 in rice caused delayed tapetal programmed cell death and abnormal development of plastids in the tapetal cells.
- OsGL1-4 is preferentially expressed in pollen and tapetal cells, and is required for the synthesis of very-long-chain alkanes.
- Gas chromatography-mass spectrometry analysis suggested the humidity-sensitive male sterility of OsGL1-4 was probably due to a significant reduction in C25 and C27 alkanes.
- OsCER1 regulates humidity-sensitive genic male sterility through very-long-chain (VLC) alkane metabolism of tryphine in rice.
- Phenotypic investigation of NPB and LTR1 suggested that LTR1 showed rumpled leaf with uneven distribution of bulliform cells and sclerenchyma cells, and disordered vascular bundles.
- A decrease in seed-setting rate in LTR1 led to decreased per-plant grain yield.
- Moreover, LTR1 was sensitive to salt stress, and LTR1 was strongly induced by salt stress.
- Moreover, overexpression of LTR1 enhanced yield in rice and LTR1 positively regulates salt stress by affecting water and ion homeostasis.
- Map-based cloning of LTR1 showed that there was a 2-bp deletion in the eighth exon of LOC_Os02g40784 in LTR1, resulting in a frameshift mutation and early termination of transcription.
Functional Keywords
- pollen , development , cell-death , tapetum , tapetal , pollen-development , tapetal-programmed-cell-death , sterility , male-sterility , leaf , vascular-bundle , grain , stress , grain-yield , map-based-cloning , salt , yield , salt-stress , homeostasis
Literature and News
- Tapetum degeneration retardation is critical for aliphatic metabolism and gene regulation during rice pollen development . DOI: 10.1093/mp/ssn028 ; PMID: 19825565
- OsCER1 Plays a Pivotal Role in Very-Long-Chain Alkane Biosynthesis and Affects Plastid Development and Programmed Cell Death of Tapetum in Rice (Oryza sativa L.) . DOI: 10.3389/fpls.2018.01217 ; PMID: 30237804
- Deficiency of very long chain alkanes biosynthesis causes humidity-sensitive male sterility via affecting pollen adhesion and hydration in rice . DOI: 10.1111/pce.13637 ; PMID: 31380565
- OsCER1 regulates humidity-sensitive genic male sterility through very-long-chain (VLC) alkane metabolism of tryphine in rice . DOI: 10.1071/FP20168 ; PMID: 33487216
- LEAF TIP RUMPLED 1 Regulates Leaf Morphology and Salt Tolerance in Rice . DOI: 10.3390/ijms23158818 ; PMID: 35955949
Gene Resources
- UniProt: Q6K9F6
- EMBL: AK066386, AP004053, AP004059
- AlphaFoldDB: Q6K9F6
- EnsemblPlants: Os02t0621300-01
- Gramene: Os02t0621300-01
- KEGG: dosa:Os02g0621300, osa:4330012
Sequences
cDNA Sequence
- >LOC_Os02g40784.1
GGTTGGGTTGGTGCTTGGCTAGCCACGGTATTATTGCGTGAAAGAGGGGGAGGTGAGGACGTGAGGTGAGGTGAGGTGAGGCAGAGTGACCACAGCGACGGCAGCGTAGCCATGGCGACCAGGCCGGGCCCTTTGACCGAATGGCCGTGGCATAGGCTGGGGAACTTCAAGTACGTGGTGATGGCCCCCGTGGTGGCGCACGGCGCGCGCCGGGTGATGCGCAACGGGTGGGGCGACCTCGACATCGCCTTCTCCCTCATCCTGCCGTCGCTGCTGCTGCGGATGATCCACAACCAGATCTGGATCAGCCTCTCCCGCTACCAGACCGCCCGCAGCAAGCACCGCATCGTCGACCGCGGCATCGAGTTCGACCAGGTCGACCGCGAGCGAGGCTGGGATGACCAGATCCTGTTCAATGGGTTGGTGTTCTACGCGGGGTACCTGGCGATGCCGAGCGTGAGGCGGATGCCGGTGTGGAGGACGGACGGCGCCGTGGTGACGGCGCTGGTGCACACGGGCCCCGTCGAGTTCCTCTACTACTGGTTCCACCGCGCGCTGCACCACCACTTCCTCTACTCCCGCTACCACTCCCACCACCACGCCTCCATCGTCACCGAGCCCATCACCTCTGTTATTCATCCTTTTGCTGAACATGTGGTCTATTTCATACTGTTTGCAATCCCCATACTGTCAACAATTTATTTGGGAAATGTTTCGGCCATGGGCATTGTGGGGTACATTGCTTACATTGACTTCATGAACAACATGGGACACTGCAATTTTGAGTTGGTGCCAGAGTGGATTTTCCAAATCTTTCCACCCCTTAAGTATCTGATCTACACCCCATCGTTCCATTCTCTTCATCATACGCAGTTCCGCACAAACTACTCGCTATTCATGCCATTTTATGACTACATATATAACACCATGGACAAGTCATCTGACGAGCTGTATGAAAGCTCACTGAAAGGAACGGAAGAAACACCTGACCTTGTTCATCTCACGCATATGACCAACTTGCAATCAGCCTATCATCTAAGGATAGGAATTGCATCCATAGCCTCCAAACCGTACAGTGACTCTGCGTGGTACATGTGGACGTTATGGCCCTTGGCATGGCTGTCAATGGTACTTGCATGGATTTATGGGTCCTCAGCATTTGTCGTTGAGAGAATTAAACTAAACAAGATGAAGATGCAAACATGGGCATTACCAAGATACAACTTCCAATATGGCCTTACGTGGGAGAGAGAACCAATCAACGACTTAATTGAAAAGGCAATATTGGATGCTGATATGAAAGGGGTTAAAGTGATTAGTCTAGGACTACTGAATCAGGCAAAGCAGCTCAATGGAAACGGTGAACTATTTAGACAGAAGTATCCAAAATTAGGAGTTCGAATTATCGACGGAAGTGGCTTAGCTACTGCAGTGGTGCTAAAAAGTATTCCTTCAGATGCAAAGAAAGTTTTTCTTCGTACAGGCACTTCTAAGATAGCTCGTGCCATTGCCATTGCACTATGTGACAGAGGTGTCCAGGTGATCATGAATGAAAAGGAGGTATACCACATGCTCAAGTCACAAATACCAGAGAACAGGGCAAGCTATTTGAAACTCTCGAGCGACAACGTACCTCAGCTTTGGATAGTACACAATATTGACGACAACGAGCAAAAGATGGCGCCAAAAGGAACAATTTTTATTCCCATATCACAGTTCCCCCTTAAGAAACTTCGCAAGGACTGCACTTACATGAGCACCCCAGCAATGAGGATCCCGGAGGAAATGAAGAATATCCACTCCTGCGAGAATTGGCTTCCAAGAAGGGTGATGAGTGCTTGGCACATTGCTGGAATACTTCATGCTCTGGAAGGATGGAACATGCACGAGTGTGGGGATGAGATGATGGATATTGAGAAATCATGGTCAGCTGCCATTCGGCATGGATTCCTTCCTCTCACTAAAGCTTGACACCTCTGGAAGCATGGATGGTGTATTCCATGCTAGTAGATGATGCTATGATTTCCAGCAACGGCATAATCCATTCATTATCTAGCTTGGGAACCAGATGGATTATTCACTTATTATCTTACTATGTTTGTAATACTGAGCTTGGGTACTGATAAGTCATGAGGAGGCACAGTGCAAACGAACTTAATTCCAATAGCTCAGTATTTTATAATCTATAGTCTATGTCAGTTCTGTAACATTACGTTTATCTCTTATTTGAATCTTGTGGGGGAATAATTAAAATAATAGGCAATGTTTTCTTGTTTAGACTTCGCAAAGTAAAACCTGACTGATCGCACGGTCAGTGCTCTTGAGACGAGACAAACAAGACCAGGTAAAGCTAAGCTTACTCGCTGGCTCGCTGCTACTATCGTTTTAGCCTTCCCTTAAAGTTTGATTCGACACTGTAAAATTCGCCCATGACGCTCTGCACAAAATCTTTGAAACTAGTGGCAACACAGCAAAATCAGATGC - >LOC_Os02g40784.3
GGTTGGGTTGGTGCTTGGCTAGCCACGGTATTATTGCGTGAAAGAGGGGGAGGTGAGGACGTGAGGTGAGGTGAGGTGAGGCAGAGTGACCACAGCGACGGCAGCGTAGCCATGGCGACCAGGCCGGGCCCTTTGACCGAATGGCCGTGGCATAGGCTGGGGAACTTCAAGTACGTGGTGATGGCCCCCGTGGTGGCGCACGGCGCGCGCCGGGTGATGCGCAACGGGTGGGGCGACCTCGACATCGCCTTCTCCCTCATCCTGCCGTCGCTGCTGCTGCGGATGATCCACAACCAGATCTGGATCAGCCTCTCCCGCTACCAGACCGCCCGCAGCAAGCACCGCATCGTCGACCGCGGCATCGAGTTCGACCAGGTCGACCGCGAGCGAGGCTGGGATGACCAGATCCTGTTCAATGGGTTGGTGTTCTACGCGGGGTACCTGGCGATGCCGAGCGTGAGGCGGATGCCGGTGTGGAGGACGGACGGCGCCGTGGTGACGGCGCTGGTGCACACGGGCCCCGTCGAGTTCCTCTACTACTGGTTCCACCGCGCGCTGCACCACCACTTCCTCTACTCCCGCTACCACTCCCACCACCACGCCTCCATCGTCACCGAGCCCATCACCTCTGTTATTCATCCTTTTGCTGAACATGTGGTCTATTTCATACTGTTTGCAATCCCCATACTGTCAACAATTTATTTGGGAAATGTTTCGGCCATGGGCATTGTGGGGTACATTGCTTACATTGACTTCATGAACAACATGGGACACTGCAATTTTGAGTTGGTGCCAGAGTGGATTTTCCAAATCTTTCCACCCCTTAAGTATCTGATCTACACCCCATCGTTCCATTCTCTTCATCATACGCAGTTCCGCACAAACTACTCGCTATTCATGCCATTTTATGACTACATATATAACACCATGGACAAGTCATCTGACGAGCTGTATGAAAGCTCACTGAAAGGAACGGAAGAAACACCTGACCTTGTTCATCTCACGCATATGACCAACTTGCAATCAGCCTATCATCTAAGGATAGGAATTGCATCCATAGCCTCCAAACCGTACAGTGACTCTGCGTGGTACATGTGGACGTTATGGCCCTTGGCATGGCTGTCAATGGTACTTGCATGGATTTATGGGTCCTCAGCATTTGTCGTTGAGAGAATTAAACTAAACAAGATGAAGATGCAAACATGGGCATTACCAAGATACAACTTCCAATATGGCCTTACGTGGGAGAGAGAACCAATCAACGACTTAATTGAAAAGGCAATATTGGATGCTGATATGAAAGGGGTTAAAGTGATTAGTCTAGGACTACTGAATCAGGCAAAGCAGCTCAATGGAAACGGTGAACTATTTAGACAGAAGTATCCAAAATTAGGAGTTCGAATTATCGACGGAAGTGGCTTAGCTACTGCAGTGGTGCTAAAAAGTATTCCTTCAGATGCAAAGAAAGTTTTTCTTCGTACAGGCACTTCTAAGATAGCTCGTGCCATTGCCATTGCACTATGTGACAGAGGTGTCCAGGTGATCATGAATGAAAAGGAGGTATACCACATGCTCAAGTCACAAATACCAGAGAACAGGGCAAGCTATTTGAAACTCTCGAGCGACAACGTACCTCAGCTTTGGATAGTACACAATATTGACGACAACGAGCAAAAGATGGCGCCAAAAGGAACAATTTTTATTCCCATATCACAGTTCCCCCTTAAGAAACTTCGCAAGGACTGCACTTACATGAGCACCCCAGCAATGAGGATCCCGGAGGAAATGAAGAATATCCACTCCTGCGAGAATTGGCTTCCAAGAAGGGTGATGAGTGCTTGGCACATTGCTGGAATACTTCATGCTCTGGAAGGATGGAACATGCACGAGTGTGGGGATGAGATGATGGATATTGAGAAATCATGGTCAGCTGCCATTCGGCATGGATTCCTTCCTCTCACTAAAGCTTGACACCTCTGGAAGCATGGATGGTGTATTCCATGCTAGTAGATGATGCTATGATTTCCAGCAACGGCATAATCCATTCATTATCTAGCTTGGGAACCAGATGGATTATTCACTTATTATCTTACTATGTTTGTAATACTGAGCTTGGGTACTGATAAGTCATGAGGAGGCACAGTGCAAACGAACTTAATTCCAATAGCTCAGTATTTTATAATCTATAGTCTATGTCAGTTCTGTAACATTACGTTTATCTCTTATTTGAATCTTGTGGGGGAATAATTAAAATAATAGGCAATGTTTTCTTGTTTAGACTTCGCAAAGTAAAACCTGACTGATCGCACGGTCAGTGCTCTTGAGACGAGACAAACAAGACCAGGTAAAGCTAAGCTTACTCGCTGGCTCGCTGCTACTATCGTTTTAGCCTTCCCTTAAAGTTTGATTCGACACTGTAAAATTCGCCCATGACGCTCTGCACAAAATCTTTGAAACTAGTGGCAACACAGCAAAATCAGATGC
CDS Sequence
- >LOC_Os02g40784.1
ATGGCGACCAGGCCGGGCCCTTTGACCGAATGGCCGTGGCATAGGCTGGGGAACTTCAAGTACGTGGTGATGGCCCCCGTGGTGGCGCACGGCGCGCGCCGGGTGATGCGCAACGGGTGGGGCGACCTCGACATCGCCTTCTCCCTCATCCTGCCGTCGCTGCTGCTGCGGATGATCCACAACCAGATCTGGATCAGCCTCTCCCGCTACCAGACCGCCCGCAGCAAGCACCGCATCGTCGACCGCGGCATCGAGTTCGACCAGGTCGACCGCGAGCGAGGCTGGGATGACCAGATCCTGTTCAATGGGTTGGTGTTCTACGCGGGGTACCTGGCGATGCCGAGCGTGAGGCGGATGCCGGTGTGGAGGACGGACGGCGCCGTGGTGACGGCGCTGGTGCACACGGGCCCCGTCGAGTTCCTCTACTACTGGTTCCACCGCGCGCTGCACCACCACTTCCTCTACTCCCGCTACCACTCCCACCACCACGCCTCCATCGTCACCGAGCCCATCACCTCTGTTATTCATCCTTTTGCTGAACATGTGGTCTATTTCATACTGTTTGCAATCCCCATACTGTCAACAATTTATTTGGGAAATGTTTCGGCCATGGGCATTGTGGGGTACATTGCTTACATTGACTTCATGAACAACATGGGACACTGCAATTTTGAGTTGGTGCCAGAGTGGATTTTCCAAATCTTTCCACCCCTTAAGTATCTGATCTACACCCCATCGTTCCATTCTCTTCATCATACGCAGTTCCGCACAAACTACTCGCTATTCATGCCATTTTATGACTACATATATAACACCATGGACAAGTCATCTGACGAGCTGTATGAAAGCTCACTGAAAGGAACGGAAGAAACACCTGACCTTGTTCATCTCACGCATATGACCAACTTGCAATCAGCCTATCATCTAAGGATAGGAATTGCATCCATAGCCTCCAAACCGTACAGTGACTCTGCGTGGTACATGTGGACGTTATGGCCCTTGGCATGGCTGTCAATGGTACTTGCATGGATTTATGGGTCCTCAGCATTTGTCGTTGAGAGAATTAAACTAAACAAGATGAAGATGCAAACATGGGCATTACCAAGATACAACTTCCAATATGGCCTTACGTGGGAGAGAGAACCAATCAACGACTTAATTGAAAAGGCAATATTGGATGCTGATATGAAAGGGGTTAAAGTGATTAGTCTAGGACTACTGAATCAGGCAAAGCAGCTCAATGGAAACGGTGAACTATTTAGACAGAAGTATCCAAAATTAGGAGTTCGAATTATCGACGGAAGTGGCTTAGCTACTGCAGTGGTGCTAAAAAGTATTCCTTCAGATGCAAAGAAAGTTTTTCTTCGTACAGGCACTTCTAAGATAGCTCGTGCCATTGCCATTGCACTATGTGACAGAGGTGTCCAGGTGATCATGAATGAAAAGGAGGTATACCACATGCTCAAGTCACAAATACCAGAGAACAGGGCAAGCTATTTGAAACTCTCGAGCGACAACGTACCTCAGCTTTGGATAGTACACAATATTGACGACAACGAGCAAAAGATGGCGCCAAAAGGAACAATTTTTATTCCCATATCACAGTTCCCCCTTAAGAAACTTCGCAAGGACTGCACTTACATGAGCACCCCAGCAATGAGGATCCCGGAGGAAATGAAGAATATCCACTCCTGCGAGAATTGGCTTCCAAGAAGGGTGATGAGTGCTTGGCACATTGCTGGAATACTTCATGCTCTGGAAGGATGGAACATGCACGAGTGTGGGGATGAGATGATGGATATTGAGAAATCATGGTCAGCTGCCATTCGGCATGGATTCCTTCCTCTCACTAAAGCTTGA - >LOC_Os02g40784.3
ATGGCGACCAGGCCGGGCCCTTTGACCGAATGGCCGTGGCATAGGCTGGGGAACTTCAAGTACGTGGTGATGGCCCCCGTGGTGGCGCACGGCGCGCGCCGGGTGATGCGCAACGGGTGGGGCGACCTCGACATCGCCTTCTCCCTCATCCTGCCGTCGCTGCTGCTGCGGATGATCCACAACCAGATCTGGATCAGCCTCTCCCGCTACCAGACCGCCCGCAGCAAGCACCGCATCGTCGACCGCGGCATCGAGTTCGACCAGGTCGACCGCGAGCGAGGCTGGGATGACCAGATCCTGTTCAATGGGTTGGTGTTCTACGCGGGGTACCTGGCGATGCCGAGCGTGAGGCGGATGCCGGTGTGGAGGACGGACGGCGCCGTGGTGACGGCGCTGGTGCACACGGGCCCCGTCGAGTTCCTCTACTACTGGTTCCACCGCGCGCTGCACCACCACTTCCTCTACTCCCGCTACCACTCCCACCACCACGCCTCCATCGTCACCGAGCCCATCACCTCTGTTATTCATCCTTTTGCTGAACATGTGGTCTATTTCATACTGTTTGCAATCCCCATACTGTCAACAATTTATTTGGGAAATGTTTCGGCCATGGGCATTGTGGGGTACATTGCTTACATTGACTTCATGAACAACATGGGACACTGCAATTTTGAGTTGGTGCCAGAGTGGATTTTCCAAATCTTTCCACCCCTTAAGTATCTGATCTACACCCCATCGTTCCATTCTCTTCATCATACGCAGTTCCGCACAAACTACTCGCTATTCATGCCATTTTATGACTACATATATAACACCATGGACAAGTCATCTGACGAGCTGTATGAAAGCTCACTGAAAGGAACGGAAGAAACACCTGACCTTGTTCATCTCACGCATATGACCAACTTGCAATCAGCCTATCATCTAAGGATAGGAATTGCATCCATAGCCTCCAAACCGTACAGTGACTCTGCGTGGTACATGTGGACGTTATGGCCCTTGGCATGGCTGTCAATGGTACTTGCATGGATTTATGGGTCCTCAGCATTTGTCGTTGAGAGAATTAAACTAAACAAGATGAAGATGCAAACATGGGCATTACCAAGATACAACTTCCAATATGGCCTTACGTGGGAGAGAGAACCAATCAACGACTTAATTGAAAAGGCAATATTGGATGCTGATATGAAAGGGGTTAAAGTGATTAGTCTAGGACTACTGAATCAGGCAAAGCAGCTCAATGGAAACGGTGAACTATTTAGACAGAAGTATCCAAAATTAGGAGTTCGAATTATCGACGGAAGTGGCTTAGCTACTGCAGTGGTGCTAAAAAGTATTCCTTCAGATGCAAAGAAAGTTTTTCTTCGTACAGGCACTTCTAAGATAGCTCGTGCCATTGCCATTGCACTATGTGACAGAGGTGTCCAGGTGATCATGAATGAAAAGGAGGTATACCACATGCTCAAGTCACAAATACCAGAGAACAGGGCAAGCTATTTGAAACTCTCGAGCGACAACGTACCTCAGCTTTGGATAGTACACAATATTGACGACAACGAGCAAAAGATGGCGCCAAAAGGAACAATTTTTATTCCCATATCACAGTTCCCCCTTAAGAAACTTCGCAAGGACTGCACTTACATGAGCACCCCAGCAATGAGGATCCCGGAGGAAATGAAGAATATCCACTCCTGCGAGAATTGGCTTCCAAGAAGGGTGATGAGTGCTTGGCACATTGCTGGAATACTTCATGCTCTGGAAGGATGGAACATGCACGAGTGTGGGGATGAGATGATGGATATTGAGAAATCATGGTCAGCTGCCATTCGGCATGGATTCCTTCCTCTCACTAAAGCTTGA
Protein Sequence
- >LOC_Os02g40784.1
MATRPGPLTEWPWHRLGNFKYVVMAPVVAHGARRVMRNGWGDLDIAFSLILPSLLLRMIHNQIWISLSRYQTARSKHRIVDRGIEFDQVDRERGWDDQILFNGLVFYAGYLAMPSVRRMPVWRTDGAVVTALVHTGPVEFLYYWFHRALHHHFLYSRYHSHHHASIVTEPITSVIHPFAEHVVYFILFAIPILSTIYLGNVSAMGIVGYIAYIDFMNNMGHCNFELVPEWIFQIFPPLKYLIYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKSSDELYESSLKGTEETPDLVHLTHMTNLQSAYHLRIGIASIASKPYSDSAWYMWTLWPLAWLSMVLAWIYGSSAFVVERIKLNKMKMQTWALPRYNFQYGLTWEREPINDLIEKAILDADMKGVKVISLGLLNQAKQLNGNGELFRQKYPKLGVRIIDGSGLATAVVLKSIPSDAKKVFLRTGTSKIARAIAIALCDRGVQVIMNEKEVYHMLKSQIPENRASYLKLSSDNVPQLWIVHNIDDNEQKMAPKGTIFIPISQFPLKKLRKDCTYMSTPAMRIPEEMKNIHSCENWLPRRVMSAWHIAGILHALEGWNMHECGDEMMDIEKSWSAAIRHGFLPLTKA* - >LOC_Os02g40784.3
MATRPGPLTEWPWHRLGNFKYVVMAPVVAHGARRVMRNGWGDLDIAFSLILPSLLLRMIHNQIWISLSRYQTARSKHRIVDRGIEFDQVDRERGWDDQILFNGLVFYAGYLAMPSVRRMPVWRTDGAVVTALVHTGPVEFLYYWFHRALHHHFLYSRYHSHHHASIVTEPITSVIHPFAEHVVYFILFAIPILSTIYLGNVSAMGIVGYIAYIDFMNNMGHCNFELVPEWIFQIFPPLKYLIYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKSSDELYESSLKGTEETPDLVHLTHMTNLQSAYHLRIGIASIASKPYSDSAWYMWTLWPLAWLSMVLAWIYGSSAFVVERIKLNKMKMQTWALPRYNFQYGLTWEREPINDLIEKAILDADMKGVKVISLGLLNQAKQLNGNGELFRQKYPKLGVRIIDGSGLATAVVLKSIPSDAKKVFLRTGTSKIARAIAIALCDRGVQVIMNEKEVYHMLKSQIPENRASYLKLSSDNVPQLWIVHNIDDNEQKMAPKGTIFIPISQFPLKKLRKDCTYMSTPAMRIPEEMKNIHSCENWLPRRVMSAWHIAGILHALEGWNMHECGDEMMDIEKSWSAAIRHGFLPLTKA*