Gene Details:

Functional Descriptions:

  • The three members of the rice OsAMT1 gene family of ammonium transporters show distinct expression patterns; constitutive and ammonium-promoted expression in shoots and roots for OsAMT1;1; root-specific and ammonium-inducible expression for OsAMT1;2; root-specific and nitrogen-repressible expression for OsAMT1;3 [Sonoda et al.
  • Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.
  • In situ mRNA detection revealed that OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.
  • Ammonium application to roots following a period of nitrogen starvation induced accumulation of OsAMT1;1 and OsAMT1;2 mRNA, but a decrease of OsAMT1;3 mRNA levels.
  • The expression patterns of the three genes showed good correlation (positive in OsAMT1;1 and OsAMT1;2, negative in OsAMT1;3) with the root tissue contents of glutamine but not of ammonium.
  • Spatial expression analysis showed that the upregulated expression of OsAMT1;1 and OsAMT1;2 and downregulated expression of OsAMT1;3 by ammonium were higher in the root mature region than in the root tips.
  • Upon exposure to ammonium, localization of OsAMT1;1 and OsAMT1;2 was also observed in the endoplasmic reticulum, but their abundance in the plasma membrane was not changed.
  • OsAMT1;1 and OsAMT1;2 Coordinate Root Morphological and Physiological Responses to Ammonium for Efficient Nitrogen Foraging in Rice.
  • The two independent double mutants (dko) defective in OsAMT1;1 and OsAMT1;2 failed to induce NH4+ uptake and stimulate LR formation, suggesting that OsAMT1s conferred the substrate-dependent root NH4+ foraging.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os02g40730.1
    ACAGCCCACACTTCCATTGCTCCTCCCCTCTCCTCTACAGTCTGTGTTGAGCGCGCGTCGAGGCGGCGAGGATGGCAACGTGCGCGGATACCCTCGGCCCGCTGCTGGGCACGGCGGCGGCGAACGCGACGGACTACCTGTGCAACCAGTTCGCGGACACGACGTCGGCCGTGGACTCGACGTACCTGCTCTTCTCGGCGTACCTCGTGTTCGCCATGCAGCTCGGCTTCGCCATGCTCTGCGCCGGGTCCGTCCGCGCCAAGAACACCATGAACATCATGCTTACCAACGTGCTCGACGCCGCCGCCGGCGCGCTCTTCTACTACCTCTTCGGCTTCGCCTTCGCCTTCGGGGCGCCGTCCAACGGCTTCATCGGGAAGCACTTCTTCGGCCTCAAGCAGGTCCCACAGGTCGGCTTCGACTACAGCTTCTTCCTCTTCCAGTGGGCCTTCGCCATCGCCGCCGCGGGCATCACGTCCGGCTCCATCGCCGAGCGGACCCAGTTCGTGGCGTACCTCATCTACTCCGCCTTCCTCACCGGCTTCGTCTACCCGGTGGTGTCCCACTGGATCTGGTCCGCCGACGGGTGGGCCTCGGCTTCCCGGACGTCGGGGTCGCTGCTCTTCGGGTCCGGCGTCATCGACTTCGCCGGGTCAGGGGTTGTCCACATGGTGGGCGGCGTGGCCGGACTCTGGGGCGCCCTCATCGAGGGCCCCCGCATTGGGCGGTTCGACCACGCCGGCCGCTCGGTGGCGCTGCGCGGCCACAGCGCGTCGCTCGTCGTGCTCGGCAGCTTCCTTCTGTGGTTCGGGTGGTACGGGTTTAACCCCGGCTCGTTCCTCACCATCCTCAAATCCTACGGCCCGCCCGGTAGCATCCACGGGCAGTGGTCGGCGGTGGGACGCACCGCCGTGACCACCACCCTCGCCGGCAGCACGGCGGCGCTCACGACGCTCTTCGGGAAGAGGCTCCAGACGGGGCACTGGAACGTGATCGACGTCTGCAACGGCCTCCTCGGCGGCTTCGCGGCGATCACCGCCGGTTGCTCCGTCGTCGACCCGTGGGCCGCGATCATCTGCGGGTTCGTCTCGGCGTGGGTGCTCATCGGCCTCAACGCGCTGGCGGCGAGGCTCAAGTTCGACGACCCGCTCGAGGCGGCGCAGCTGCACGGCGGGTGCGGCGCGTGGGGGGTCATCTTCACGGCGCTGTTCGCGCGCAAGGAGTACGTGGACCAGATCTTCGGCCAGCCCGGGCGCCCGTACGGGCTGTTCATGGGCGGCGGCGGCCGGCTGCTCGGGGCGCACATAGTGGTCATCCTGGTCATCGCGGCGTGGGTGAGCTTCACCATGGCGCCGCTGTTCCTGGTGCTCAACAAGCTGGGCTTGCTGCGCATCTCGGCCGAGGACGAGATGGCCGGCATGGACCAGACGCGCCACGGCGGGTTCGCGTACGCGTACCACGACGACGACGCGAGCGGCAAGCCGGACCGCAGCGTCGGCGGGTTCATGCTCAAGTCGGCGCACGGCACGCAGGTCGCCGCCGAGATGGGAGGCCATGTCTAGTGGAACCGGAGGAGCTGAGCTAGTAGTACATACATGCAGCATCATCGATCGAACGAAATGCATATAAGCGTTTTTCAAGGTTGATCTGATGCTGCAGGTTTCGTGATTGTATAATAGGAAGAAAAAGATAGTAGTATTTTTTATCTGAGATCATCTGTTTGGAACAGGGGATTTGACTAAGATTTGATATAAATTTACACAAAATCTTAGCAAAAATCCCTTTATCTCAACTCTCAAGTAGAGCTTTGCTTTGTACAACAAAGTATCATGTGTGATATAATTGTCAGGTGGAAAAACTGGAAACGGGTATAATCTACCTTTCTTTGTCAATGATCGGGTGACCACATGAGGAGAGAGCCCGTGTTTGCTATCAGGTTGAAAAAAAAAACCATACGTAATAATAAATAAAAGGTTGACAACTGTGGTATTTGTAGTTTGT
CDS Sequence
  • >LOC_Os02g40730.1
    ATGGCAACGTGCGCGGATACCCTCGGCCCGCTGCTGGGCACGGCGGCGGCGAACGCGACGGACTACCTGTGCAACCAGTTCGCGGACACGACGTCGGCCGTGGACTCGACGTACCTGCTCTTCTCGGCGTACCTCGTGTTCGCCATGCAGCTCGGCTTCGCCATGCTCTGCGCCGGGTCCGTCCGCGCCAAGAACACCATGAACATCATGCTTACCAACGTGCTCGACGCCGCCGCCGGCGCGCTCTTCTACTACCTCTTCGGCTTCGCCTTCGCCTTCGGGGCGCCGTCCAACGGCTTCATCGGGAAGCACTTCTTCGGCCTCAAGCAGGTCCCACAGGTCGGCTTCGACTACAGCTTCTTCCTCTTCCAGTGGGCCTTCGCCATCGCCGCCGCGGGCATCACGTCCGGCTCCATCGCCGAGCGGACCCAGTTCGTGGCGTACCTCATCTACTCCGCCTTCCTCACCGGCTTCGTCTACCCGGTGGTGTCCCACTGGATCTGGTCCGCCGACGGGTGGGCCTCGGCTTCCCGGACGTCGGGGTCGCTGCTCTTCGGGTCCGGCGTCATCGACTTCGCCGGGTCAGGGGTTGTCCACATGGTGGGCGGCGTGGCCGGACTCTGGGGCGCCCTCATCGAGGGCCCCCGCATTGGGCGGTTCGACCACGCCGGCCGCTCGGTGGCGCTGCGCGGCCACAGCGCGTCGCTCGTCGTGCTCGGCAGCTTCCTTCTGTGGTTCGGGTGGTACGGGTTTAACCCCGGCTCGTTCCTCACCATCCTCAAATCCTACGGCCCGCCCGGTAGCATCCACGGGCAGTGGTCGGCGGTGGGACGCACCGCCGTGACCACCACCCTCGCCGGCAGCACGGCGGCGCTCACGACGCTCTTCGGGAAGAGGCTCCAGACGGGGCACTGGAACGTGATCGACGTCTGCAACGGCCTCCTCGGCGGCTTCGCGGCGATCACCGCCGGTTGCTCCGTCGTCGACCCGTGGGCCGCGATCATCTGCGGGTTCGTCTCGGCGTGGGTGCTCATCGGCCTCAACGCGCTGGCGGCGAGGCTCAAGTTCGACGACCCGCTCGAGGCGGCGCAGCTGCACGGCGGGTGCGGCGCGTGGGGGGTCATCTTCACGGCGCTGTTCGCGCGCAAGGAGTACGTGGACCAGATCTTCGGCCAGCCCGGGCGCCCGTACGGGCTGTTCATGGGCGGCGGCGGCCGGCTGCTCGGGGCGCACATAGTGGTCATCCTGGTCATCGCGGCGTGGGTGAGCTTCACCATGGCGCCGCTGTTCCTGGTGCTCAACAAGCTGGGCTTGCTGCGCATCTCGGCCGAGGACGAGATGGCCGGCATGGACCAGACGCGCCACGGCGGGTTCGCGTACGCGTACCACGACGACGACGCGAGCGGCAAGCCGGACCGCAGCGTCGGCGGGTTCATGCTCAAGTCGGCGCACGGCACGCAGGTCGCCGCCGAGATGGGAGGCCATGTCTAG
Protein Sequence
  • >LOC_Os02g40730.1
    MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIGKHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVYPVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIGRFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAVGRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAIICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQPGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGMDQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV*