Information report for OsAMT1;3;OsAMT1.3
Gene Details
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Functional Descriptions
- Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.
- The three members of the rice OsAMT1 gene family of ammonium transporters show distinct expression patterns; constitutive and ammonium-promoted expression in shoots and roots for OsAMT1;1; root-specific and ammonium-inducible expression for OsAMT1;2; root-specific and nitrogen-repressible expression for OsAMT1;3 [Sonoda et al.
- Ammonium application to roots following a period of nitrogen starvation induced accumulation of OsAMT1;1 and OsAMT1;2 mRNA, but a decrease of OsAMT1;3 mRNA levels.
- The expression patterns of the three genes showed good correlation (positive in OsAMT1;1 and OsAMT1;2, negative in OsAMT1;3) with the root tissue contents of glutamine but not of ammonium.
- In this report, we demonstrate that the rice root expressed OsAMT1;3 (Oryza sativa ammonium transporter 1;3) functions as a typical high-affinity NH4(+) transporter and is weakly permeable to MeA(+) using growth assays in NH4(+) uptake defective yeast cells and electrophysiological measurements in Xenopus oocytes.
- Spatial expression analysis showed that the upregulated expression of OsAMT1;1 and OsAMT1;2 and downregulated expression of OsAMT1;3 by ammonium were higher in the root mature region than in the root tips.
Functional Keywords
- root , shoot , nitrogen , transporter , growth
Literature and News
- Differential expression of three members of the AMT1 gene family encoding putative high-affinity NH4+ transporters in roots of Oryza sativa subspecies indica . DOI: 10.1046/j.1365-3040.2003.01023.x ; PMID: 12803618
- Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice . DOI: 10.1093/pcp/pcg169 ; PMID: 14701935
- Distinct expression and function of three ammonium transporter genes (OsAMT1;1-1;3) in rice . DOI: 10.1093/pcp/pcg083 ; PMID: 12881500
- Identification of structural elements involved in fine-tuning of the transport activity of the rice ammonium transporter OsAMT1;3 . DOI: 10.1016/j.plaphy.2016.07.003 ; PMID: 27423220
- New Insights into the Transcriptional Regulation of Genes Involved in the Nitrogen Use Efficiency under Potassium Chlorate in Rice (Oryza sativa L.) . DOI: 10.3390/ijms22042192 ; PMID: 33671842
- Three polarly localized ammonium transporter 1 members are cooperatively responsible for ammonium uptake in rice under low ammonium condition . DOI: 10.1111/nph.17679 ; PMID: 34392543
- Mutation of phytochrome B promotes resistance to sheath blight and saline-alkaline stress via increasing ammonium uptake in rice . DOI: 10.1111/tpj.16046 ; PMID: 36440495
Gene Resources
- UniProt: Q6K9G3
- EMBL: AF289478, AP004053, AP004059
- AlphaFoldDB: Q6K9G3
- EnsemblPlants: Os02t0620500-01
- Gramene: Os02t0620500-01
- KEGG: dosa:Os02g0620500
- Orthologous matrix: VVWVLMD
- InterPro: IPR001905, IPR018047, IPR024041
- PANTHER: PTHR11730, PTHR11730:SF121
- SUPFAM: SSF111352
- PROSITE: PS01219
- Gene3D: 1.10.3430.10
- OrthoDB: Q6K9G3
- SWISS-MODEL: Q6K9G3
- eggNOG: KOG0682
Sequences
cDNA Sequence
- >LOC_Os02g40710.1
CAAAACCTCCATTAGTTCCTAAACCCTAGCAAGAAGCAGCACAAAACCTTGCCACACTTGGCTAGTGAGACACACCATGGCGACGTGCTTGGACAGCCTCGGGCCGCTGCTCGGCGGCGCGGCGAACTCCACCGACGCGGCCAACTACATCTGCAACAGGTTCACGGACACCTCCTCCGCGGTGGACGCGACGTACCTGCTCTTCTCGGCCTACCTCGTGTTCGCCATGCAGCTCGGGTTCGCCATGCTCTGCGCGGGCTCCGTCCGCGCCAAGAACTCCATGAACATCATGCTCACCAACGTGCTCGACGCCGCCGCCGGCGCGCTCTTCTACTACCTCTTCGGCTTCGCCTTCGCGTTCGGGACGCCGTCCAAGGGCTTCATCGGGAAGCAGTTCTTCGGGCTGAAGCACATGCCGCAGACAGGGTACGACTACGACTTCTTCCTCTTCCAGTGGGCCTTCGCCATCGCCGCCGCCGGCATCACGTCCGGTTCCATCGCCGAGCGGACGCGCTTCAGCGCGTATCTCATCTACTCCGCCTTCCTCACCGGGTTCGTGTACCCGGTGGTGTCGCACTGGTTCTGGTCCACCGACGGGTGGGCTTCGGCCGGCCGGTTGACGGGTCCGTTGCTGTTCAAGTCGGGCGTCATCGACTTCGCCGGCTCCGGCGTCGTCCATCTGGTCGGTGGCATTGCTGGCCTGTGGGGTGCCTTCATCGAGGGCCCTCGCATCGGGCGGTTCGACGCCGCCGGCCGCACGGTGGCGATGAAAGGGCACAGCGCCTCACTGGTCGTGCTCGGCACCTTCCTGCTGTGGTTCGGGTGGTTCGGCTTCAACCCGGGGTCCTTCACCACCATCTCCAAGATCTACGGCGAGTCGGGCACGATCGACGGGCAGTGGTCGGCGGTGGGCCGCACCGCCGTGACGACGTCGCTGGCGGGGAGCGTCGCCGCGCTGACGACGCTCTACGGCAAGAGATGGCTGACGGGGCACTGGAACGTGACCGACGTCTGCAACGGTCTCCTCGGCGGCTTCGCCGCGATCACCGCGGGCTGCTCCGTGGTCGACCCGTGGGCGTCGGTGATCTGCGGGTTCGTGTCGGCGTGGGTCCTCATCGGCTGCAACAAGCTGTCGCTGATTCTCAAGTTCGACGACCCGCTGGAGGCGACGCAGCTGCACGCCGGGTGCGGCGCGTGGGGGATCATCTTCACCGCGCTGTTCGCGCGCAGGGAGTACGTCGAGCTGATCTACGGGGTGCCGGGGAGGCCGTACGGGCTGTTCATGGGCGGCGGCGGGAGGCTTCTCGCGGCGCACATCGTGCAGATCCTGGTGATCGTCGGGTGGGTCAGCGCCACCATGGGGACGCTCTTCTACGTGCTGCACAGGTTCGGGCTGCTCCGCGTCTCGCCCGCGACAGAGATGGAAGGCATGGACCCGACGTGCCACGGCGGGTTCGGGTACGTGGACGAGGACGAAGGCGAGCGCCGCGTCAGGGCCAAGTCGGCGGCGGAGACGGCTCGCGTGGAGCCCAGAAAGTCGCCGGAGCAAGCCGCGGCGGGCCAGTTTGTGTAG
CDS Sequence
- >LOC_Os02g40710.1
ATGGCGACGTGCTTGGACAGCCTCGGGCCGCTGCTCGGCGGCGCGGCGAACTCCACCGACGCGGCCAACTACATCTGCAACAGGTTCACGGACACCTCCTCCGCGGTGGACGCGACGTACCTGCTCTTCTCGGCCTACCTCGTGTTCGCCATGCAGCTCGGGTTCGCCATGCTCTGCGCGGGCTCCGTCCGCGCCAAGAACTCCATGAACATCATGCTCACCAACGTGCTCGACGCCGCCGCCGGCGCGCTCTTCTACTACCTCTTCGGCTTCGCCTTCGCGTTCGGGACGCCGTCCAAGGGCTTCATCGGGAAGCAGTTCTTCGGGCTGAAGCACATGCCGCAGACAGGGTACGACTACGACTTCTTCCTCTTCCAGTGGGCCTTCGCCATCGCCGCCGCCGGCATCACGTCCGGTTCCATCGCCGAGCGGACGCGCTTCAGCGCGTATCTCATCTACTCCGCCTTCCTCACCGGGTTCGTGTACCCGGTGGTGTCGCACTGGTTCTGGTCCACCGACGGGTGGGCTTCGGCCGGCCGGTTGACGGGTCCGTTGCTGTTCAAGTCGGGCGTCATCGACTTCGCCGGCTCCGGCGTCGTCCATCTGGTCGGTGGCATTGCTGGCCTGTGGGGTGCCTTCATCGAGGGCCCTCGCATCGGGCGGTTCGACGCCGCCGGCCGCACGGTGGCGATGAAAGGGCACAGCGCCTCACTGGTCGTGCTCGGCACCTTCCTGCTGTGGTTCGGGTGGTTCGGCTTCAACCCGGGGTCCTTCACCACCATCTCCAAGATCTACGGCGAGTCGGGCACGATCGACGGGCAGTGGTCGGCGGTGGGCCGCACCGCCGTGACGACGTCGCTGGCGGGGAGCGTCGCCGCGCTGACGACGCTCTACGGCAAGAGATGGCTGACGGGGCACTGGAACGTGACCGACGTCTGCAACGGTCTCCTCGGCGGCTTCGCCGCGATCACCGCGGGCTGCTCCGTGGTCGACCCGTGGGCGTCGGTGATCTGCGGGTTCGTGTCGGCGTGGGTCCTCATCGGCTGCAACAAGCTGTCGCTGATTCTCAAGTTCGACGACCCGCTGGAGGCGACGCAGCTGCACGCCGGGTGCGGCGCGTGGGGGATCATCTTCACCGCGCTGTTCGCGCGCAGGGAGTACGTCGAGCTGATCTACGGGGTGCCGGGGAGGCCGTACGGGCTGTTCATGGGCGGCGGCGGGAGGCTTCTCGCGGCGCACATCGTGCAGATCCTGGTGATCGTCGGGTGGGTCAGCGCCACCATGGGGACGCTCTTCTACGTGCTGCACAGGTTCGGGCTGCTCCGCGTCTCGCCCGCGACAGAGATGGAAGGCATGGACCCGACGTGCCACGGCGGGTTCGGGTACGTGGACGAGGACGAAGGCGAGCGCCGCGTCAGGGCCAAGTCGGCGGCGGAGACGGCTCGCGTGGAGCCCAGAAAGTCGCCGGAGCAAGCCGCGGCGGGCCAGTTTGTGTAG
Protein Sequence
- >LOC_Os02g40710.1
MATCLDSLGPLLGGAANSTDAANYICNRFTDTSSAVDATYLLFSAYLVFAMQLGFAMLCAGSVRAKNSMNIMLTNVLDAAAGALFYYLFGFAFAFGTPSKGFIGKQFFGLKHMPQTGYDYDFFLFQWAFAIAAAGITSGSIAERTRFSAYLIYSAFLTGFVYPVVSHWFWSTDGWASAGRLTGPLLFKSGVIDFAGSGVVHLVGGIAGLWGAFIEGPRIGRFDAAGRTVAMKGHSASLVVLGTFLLWFGWFGFNPGSFTTISKIYGESGTIDGQWSAVGRTAVTTSLAGSVAALTTLYGKRWLTGHWNVTDVCNGLLGGFAAITAGCSVVDPWASVICGFVSAWVLIGCNKLSLILKFDDPLEATQLHAGCGAWGIIFTALFARREYVELIYGVPGRPYGLFMGGGGRLLAAHIVQILVIVGWVSATMGTLFYVLHRFGLLRVSPATEMEGMDPTCHGGFGYVDEDEGERRVRAKSAAETARVEPRKSPEQAAAGQFV*