Gene Details:

Functional Descriptions:

  • Quantitative real-time RT-PCR analysis indicated that rice SDS and RCK are expressed preferentially in young flowers, and transgenic RNAi rice lines with reduced expression of these genes exhibited normal vegetative development, but showed significantly reduced fertility with partially sterile flowers and defective pollens.
  • The Arabidopsis SDS (SOLO DANCERS) and RCK (ROCK-N-ROLLERS) genes are important for male meiosis, but it is still unknown whether they represent conserved functions in plants.
  • Functional conservation of the meiotic genes SDS and RCK in male meiosis in the monocot rice.
  • Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1.
  • The null mutation of MER3 results in complete sterility without any vegetative defects.
  • Because rice and Arabidopsis, respectively, are members of monocots and eudicots, two largest groups of flowering plants, our results suggest that the functions of SDS and RCK are likely conserved in flowering plants.
  • MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice.
  • Further cytological examination of male meiocytes revealed that the SDS deficiency led to defects in homolog interaction and bivalent formation in meiotic prophase I, and RCK deficiency resulted in defective meiotic crossover formation.
  • OsMSH5 can be loaded onto meiotic chromosomes in Oszip4, OsMER3, and hei10.
  • In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes.
  • Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal.
  • Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and OsMER3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants.
  • Cytological analyses show that chiasma frequency is reduced dramatically in MER3 mutants and the remaining chiasmata distribute randomly among different pollen mother cells, implying possible coexistence of two kinds of crossover in rice.
  • In contrast, in pair2, OsMER3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os02g40450.1
    AGTAGCGCTAGCACGAACAGGCGTGAGCCAGCTTCTAACGCAAACCAGAATTTGAAATTCCTTTCCGTTGCAATCATCCACGGCTCATCTCCTCCGCCTCCTCCTCCTCACCTCGAGACACCAGAGGCATTATCCGCGCGCGGCTTCTCCTGCCATTCCTTCTCCCACCTTCGTTCCCTCGCGAAGCAGCCGCTCCTCTCTCTCGCCATCTCTCCGACTCCTCCGGTCCAAATCCCCCCCCCCCCCCTCCAAATCCGGTTCCACCAACCAGCAGTAAGCAAGCCTCTCCCTCTCCGCCTCTCGTCTTCCTACGCTGCGCGCCTCCGCCCGCTCGGGACCGCACGCGATGGCGGCGATGGGACATCTCGGCGACCCGTACGCGCTGCGCTCCGTCGCGGACCTTCCCCCGCCATTCCGCTCCGTTTTCGGGTTCAGGTATTTCAATTCGTTGCAGAGCGAGTGCTTCCCTGCATGCTTCCTCTCGGATGTAAACATGGTTATCTCTGCGCCTACTGGGAGTGGCAAAACCGTGCTGTTCGAGCTCTGCATTCTAAGGCTTCTCTCAAGGTTCCTCTCATCAGAGTGGAGGTTTAATTTGATCAAAGGAACCCTGAAAACAATCTATATTGCTCCAATGAAGGCGTTGGTGCAGGAGAAGTTGCGTGACTGGAACATGAAGCTGGGCTCGTTGGGGATCAGTTGCTTGGAGATGACTGGTGACAATGAATTCTATAACACCAAATCCATACATGATGCTGACTTAATTCTCACAACTCCTGAGAAGTTTGATTCTGTGAGTCGTCATGGAATAAGAGATGGTGGACTGGGTTTCTTCAGTGACATTGCTCTGGTCCTTATTGATGAAGTTCATCTCCTCAACGATCCTCGTGGAGCTGCCCTAGAAGCAATTGTCAGTAGAATAAAAATGCTTTCTCGACTTGGCACAATGAAGATTGCTCCTTTGGCAAATGTTCGATTCATAGCAGTTTCTGCCACTATCCCTAATATTGAGGATATAGCGGAGTGGCTTGCAGTACCGTCTGAAGGAATAAAAAGATTTGGAGAAGAGATGAGGCCAGTGAAGTTGACAACCAAGGTTTTTGGTTATGCTCCAGCAAGAAATGACTTCCTGTTTGAGAGGAGACTCCAAAGTTTCATTTTCGATATACTGATGCAACATTCAAGAGGAAAGTCTGCACTTGTTTTCTGTTCTACTAGAAAAGGCGCACAAGAAGCAGCACAGTGCCTTTCACAAACTGCGAGTTCTCTAGGCTATTCAAATCCATTTATGAAATCGATGCAGCAGTATGAACATCTAAAGGAGGCTGCACTAACCTGTAGCGACAAGCAGTTGCAGGCTTGTCTCGTACATGGAGTTGGTTATCACAACGGTGGTCTTTGCCTAAAGGATAGGAGTGTCGTTGAGGGACTTTTCCTGAAGGGTGATATTCAAATTCTTTGCACGACGAATACTTTGGCACATGGGATCAACTTACCTGCTCACACAGTGGTGATAAAGTCAACACAATTTTTCAACAAAGAGAAGGGTCTGTATGTAGAATATGAGAGATCCATGGTTCTTCAGATGTGTGGAAGGGCTGGGCGTCCACCATTTGATGATACTGGAACAATTATAATCATGACAAGAAGAGAGACAGTGCATCTATATGAGAACCTTCTAAATGGATGTGAGATGGTGGAGTCTCAGTTACTTCCATGTGCGGTTGAGCATCTAAATGCAGAGATTGTTCAGCTGACGGTGTCTGATATAACTTTGGCTATTGAGTGGCTCAAGTGCTCGTATTTGTACATCAGGATAAAGAAGAATCCTCAGCATTACGGAATTAAGAAAGAAATTCCACGTGAACTTCTTGAAAAGCAAATGAAAGATATATGTGTTGAGAAGATCCATGAGCTGGGGGAGTATGGTCTAATTTGGACAGATGAAGATGGTTTCCTTCTAAAACCATTAGAACCTGGGAGGCTGATGACAAAATTCTATCTGAAATTTGATACTATGAAGCTTATTGTCAAAGCTTCTGCCTGTTGCACTTTGGAAGATCTGTTGCATATCATCTGCCACTCTGCAGAGATTACTTGGATCCAACTACGACGAAATGAGAAGAAGCTCCTTAATGAGATAAATGCTGACAAAGAGGGAAGGCTTTGGTTCCATGTTGTTGGTGCAAATGGGAAAAGGAAGAAGCGTATTCAAACAAGAGAAGAAAAAATATTTATTCTGGCAAATGATTGTTTAACTGGGGACCCTCTAGTCCATGATCTATCTCTCAACCAGGAAATGAATTCAATATGCTCAAATGGATGCAGGGTTGCCAAATGCATGAGAGAATATTTTATATACAAAAAGAATTACAAGTCTGCCATAAGTTCTATGCTCCTTGCGAAATGCTTACACCAAAAACTTTGGGAGAGTAGTCCATTTTTGCTGAAGCAATTGCCTGGAATTGGAATTGTCACAGCAAAGGCACTGAAGACTGCTGGAATTGATTCTTTTGAGAGTTTGGCAACTGCTGATGCTAGGAAGATAGAATCCGTAACAGGACGAAATTATCCATTTGGAGATAGCATAAAAAGTTATTTGCCATCATTAGGCCCCAAAATTGATATAAACATAGAGGATGCTGGAAACAGACAGGGAAAATCAACAATTATTGTAACACTGACTCGCCTATCACAGGCAGTAGGATCCAGTAAACAAAATTACGCTGATATGGTTGTGGGATCAGAGGAAGATAATGCGATCCTCTTTCATGAGAAAATAAATCCATACTCTGTCAAACTGTATGTCCCATGCCCCCCAAATGCCAGGGCTACTCTGAAGGTTGACGTCATTTTTGAAGAATATGTTGGTCTTGATATCCACAAGAAGCATGTGGTCAGCAGGGAAGATTTTCATGTGACCAAAGTGTTTGGGATTAAAAAGGCAGAACCCTTGTACAATCTGCCTGCAGAGAGCTGTTTGGTTAGCTCTAAAACAACTCGGACAAATCAATCTAAGTATCATAATGGACAGAACCCACTTTCTAAAGAGGTATGTGTTATAGAAGATGATTTTAGGGCCAAAGCTCCAGACAAAGATGATAATGATCTGGAAATTCTGGGAACAAGAGAGTACAACAACTTAGCTTCACTGGAGGCCCCAAGCTTTACTCTACTGCATGAAGAAGATTATGAAGATGTTCCAGATGTATTGGCTTCTGAACCAGTAGAGGCAGAATGCAAAAGTGCCACCAACAACACAATTTTTGATCACATACGCAAGAAATCCAGAGATTTCCCCAATCTAATGTTGTCGAAATCCATGGATAGTTCTTATGAACCTTTGATACTGAAGAAAATGAAGACATCGGGCGATCAGTTTGGACTAGATCAGAGCAGTCTGCATGCATATGAAGTCACGCCTATGGTCTTTGATCGTACTGAGGCTAGAGTCTCTCCAAACAATACAGATGAGAGGTGCCGCAACATCCTGACCAGAACTGCAGAAACTAGATCCTTTCAGTTTACGGGCAAAATGGACAGTATATCTCAGAAGAGCGAGATCCTGAACAGAACTCAAGGCAAAAACTCTACTCAACTTGCGGGTAAAAAAGCATTTGAAAAGAGCAAGACCCCAGATGAGAATTCACTTCACTTTGTAGGTAAAAGGGACAGCTCGTCTGAGAAAAGCAAGGCTCTAAGCAAGACTCCAGATGAGAATTCTCTTCAGTTTTTAGGTAAAATGGACAGCTCATCTGAGAAGAGCAAATTCTGCTTCAGCAGTCCTCTTGCAGATTTCCAGCCTATGCAATGTACAAAGCAAGTTGCAGCCTCAGTCCAACCTCTCACGATTCAGGATTATTGCAAGGATATTTTGGCGAGTGCAAAGAGCAGTGGGACTGGCGCATCTTTTCTTGACGTTAAGAGTGTCTTCTCATTTCTCTGA
CDS Sequence
  • >LOC_Os02g40450.1
    ATGGCGGCGATGGGACATCTCGGCGACCCGTACGCGCTGCGCTCCGTCGCGGACCTTCCCCCGCCATTCCGCTCCGTTTTCGGGTTCAGGTATTTCAATTCGTTGCAGAGCGAGTGCTTCCCTGCATGCTTCCTCTCGGATGTAAACATGGTTATCTCTGCGCCTACTGGGAGTGGCAAAACCGTGCTGTTCGAGCTCTGCATTCTAAGGCTTCTCTCAAGGTTCCTCTCATCAGAGTGGAGGTTTAATTTGATCAAAGGAACCCTGAAAACAATCTATATTGCTCCAATGAAGGCGTTGGTGCAGGAGAAGTTGCGTGACTGGAACATGAAGCTGGGCTCGTTGGGGATCAGTTGCTTGGAGATGACTGGTGACAATGAATTCTATAACACCAAATCCATACATGATGCTGACTTAATTCTCACAACTCCTGAGAAGTTTGATTCTGTGAGTCGTCATGGAATAAGAGATGGTGGACTGGGTTTCTTCAGTGACATTGCTCTGGTCCTTATTGATGAAGTTCATCTCCTCAACGATCCTCGTGGAGCTGCCCTAGAAGCAATTGTCAGTAGAATAAAAATGCTTTCTCGACTTGGCACAATGAAGATTGCTCCTTTGGCAAATGTTCGATTCATAGCAGTTTCTGCCACTATCCCTAATATTGAGGATATAGCGGAGTGGCTTGCAGTACCGTCTGAAGGAATAAAAAGATTTGGAGAAGAGATGAGGCCAGTGAAGTTGACAACCAAGGTTTTTGGTTATGCTCCAGCAAGAAATGACTTCCTGTTTGAGAGGAGACTCCAAAGTTTCATTTTCGATATACTGATGCAACATTCAAGAGGAAAGTCTGCACTTGTTTTCTGTTCTACTAGAAAAGGCGCACAAGAAGCAGCACAGTGCCTTTCACAAACTGCGAGTTCTCTAGGCTATTCAAATCCATTTATGAAATCGATGCAGCAGTATGAACATCTAAAGGAGGCTGCACTAACCTGTAGCGACAAGCAGTTGCAGGCTTGTCTCGTACATGGAGTTGGTTATCACAACGGTGGTCTTTGCCTAAAGGATAGGAGTGTCGTTGAGGGACTTTTCCTGAAGGGTGATATTCAAATTCTTTGCACGACGAATACTTTGGCACATGGGATCAACTTACCTGCTCACACAGTGGTGATAAAGTCAACACAATTTTTCAACAAAGAGAAGGGTCTGTATGTAGAATATGAGAGATCCATGGTTCTTCAGATGTGTGGAAGGGCTGGGCGTCCACCATTTGATGATACTGGAACAATTATAATCATGACAAGAAGAGAGACAGTGCATCTATATGAGAACCTTCTAAATGGATGTGAGATGGTGGAGTCTCAGTTACTTCCATGTGCGGTTGAGCATCTAAATGCAGAGATTGTTCAGCTGACGGTGTCTGATATAACTTTGGCTATTGAGTGGCTCAAGTGCTCGTATTTGTACATCAGGATAAAGAAGAATCCTCAGCATTACGGAATTAAGAAAGAAATTCCACGTGAACTTCTTGAAAAGCAAATGAAAGATATATGTGTTGAGAAGATCCATGAGCTGGGGGAGTATGGTCTAATTTGGACAGATGAAGATGGTTTCCTTCTAAAACCATTAGAACCTGGGAGGCTGATGACAAAATTCTATCTGAAATTTGATACTATGAAGCTTATTGTCAAAGCTTCTGCCTGTTGCACTTTGGAAGATCTGTTGCATATCATCTGCCACTCTGCAGAGATTACTTGGATCCAACTACGACGAAATGAGAAGAAGCTCCTTAATGAGATAAATGCTGACAAAGAGGGAAGGCTTTGGTTCCATGTTGTTGGTGCAAATGGGAAAAGGAAGAAGCGTATTCAAACAAGAGAAGAAAAAATATTTATTCTGGCAAATGATTGTTTAACTGGGGACCCTCTAGTCCATGATCTATCTCTCAACCAGGAAATGAATTCAATATGCTCAAATGGATGCAGGGTTGCCAAATGCATGAGAGAATATTTTATATACAAAAAGAATTACAAGTCTGCCATAAGTTCTATGCTCCTTGCGAAATGCTTACACCAAAAACTTTGGGAGAGTAGTCCATTTTTGCTGAAGCAATTGCCTGGAATTGGAATTGTCACAGCAAAGGCACTGAAGACTGCTGGAATTGATTCTTTTGAGAGTTTGGCAACTGCTGATGCTAGGAAGATAGAATCCGTAACAGGACGAAATTATCCATTTGGAGATAGCATAAAAAGTTATTTGCCATCATTAGGCCCCAAAATTGATATAAACATAGAGGATGCTGGAAACAGACAGGGAAAATCAACAATTATTGTAACACTGACTCGCCTATCACAGGCAGTAGGATCCAGTAAACAAAATTACGCTGATATGGTTGTGGGATCAGAGGAAGATAATGCGATCCTCTTTCATGAGAAAATAAATCCATACTCTGTCAAACTGTATGTCCCATGCCCCCCAAATGCCAGGGCTACTCTGAAGGTTGACGTCATTTTTGAAGAATATGTTGGTCTTGATATCCACAAGAAGCATGTGGTCAGCAGGGAAGATTTTCATGTGACCAAAGTGTTTGGGATTAAAAAGGCAGAACCCTTGTACAATCTGCCTGCAGAGAGCTGTTTGGTTAGCTCTAAAACAACTCGGACAAATCAATCTAAGTATCATAATGGACAGAACCCACTTTCTAAAGAGGTATGTGTTATAGAAGATGATTTTAGGGCCAAAGCTCCAGACAAAGATGATAATGATCTGGAAATTCTGGGAACAAGAGAGTACAACAACTTAGCTTCACTGGAGGCCCCAAGCTTTACTCTACTGCATGAAGAAGATTATGAAGATGTTCCAGATGTATTGGCTTCTGAACCAGTAGAGGCAGAATGCAAAAGTGCCACCAACAACACAATTTTTGATCACATACGCAAGAAATCCAGAGATTTCCCCAATCTAATGTTGTCGAAATCCATGGATAGTTCTTATGAACCTTTGATACTGAAGAAAATGAAGACATCGGGCGATCAGTTTGGACTAGATCAGAGCAGTCTGCATGCATATGAAGTCACGCCTATGGTCTTTGATCGTACTGAGGCTAGAGTCTCTCCAAACAATACAGATGAGAGGTGCCGCAACATCCTGACCAGAACTGCAGAAACTAGATCCTTTCAGTTTACGGGCAAAATGGACAGTATATCTCAGAAGAGCGAGATCCTGAACAGAACTCAAGGCAAAAACTCTACTCAACTTGCGGGTAAAAAAGCATTTGAAAAGAGCAAGACCCCAGATGAGAATTCACTTCACTTTGTAGGTAAAAGGGACAGCTCGTCTGAGAAAAGCAAGGCTCTAAGCAAGACTCCAGATGAGAATTCTCTTCAGTTTTTAGGTAAAATGGACAGCTCATCTGAGAAGAGCAAATTCTGCTTCAGCAGTCCTCTTGCAGATTTCCAGCCTATGCAATGTACAAAGCAAGTTGCAGCCTCAGTCCAACCTCTCACGATTCAGGATTATTGCAAGGATATTTTGGCGAGTGCAAAGAGCAGTGGGACTGGCGCATCTTTTCTTGACGTTAAGAGTGTCTTCTCATTTCTCTGA
Protein Sequence
  • >LOC_Os02g40450.1
    MAAMGHLGDPYALRSVADLPPPFRSVFGFRYFNSLQSECFPACFLSDVNMVISAPTGSGKTVLFELCILRLLSRFLSSEWRFNLIKGTLKTIYIAPMKALVQEKLRDWNMKLGSLGISCLEMTGDNEFYNTKSIHDADLILTTPEKFDSVSRHGIRDGGLGFFSDIALVLIDEVHLLNDPRGAALEAIVSRIKMLSRLGTMKIAPLANVRFIAVSATIPNIEDIAEWLAVPSEGIKRFGEEMRPVKLTTKVFGYAPARNDFLFERRLQSFIFDILMQHSRGKSALVFCSTRKGAQEAAQCLSQTASSLGYSNPFMKSMQQYEHLKEAALTCSDKQLQACLVHGVGYHNGGLCLKDRSVVEGLFLKGDIQILCTTNTLAHGINLPAHTVVIKSTQFFNKEKGLYVEYERSMVLQMCGRAGRPPFDDTGTIIIMTRRETVHLYENLLNGCEMVESQLLPCAVEHLNAEIVQLTVSDITLAIEWLKCSYLYIRIKKNPQHYGIKKEIPRELLEKQMKDICVEKIHELGEYGLIWTDEDGFLLKPLEPGRLMTKFYLKFDTMKLIVKASACCTLEDLLHIICHSAEITWIQLRRNEKKLLNEINADKEGRLWFHVVGANGKRKKRIQTREEKIFILANDCLTGDPLVHDLSLNQEMNSICSNGCRVAKCMREYFIYKKNYKSAISSMLLAKCLHQKLWESSPFLLKQLPGIGIVTAKALKTAGIDSFESLATADARKIESVTGRNYPFGDSIKSYLPSLGPKIDINIEDAGNRQGKSTIIVTLTRLSQAVGSSKQNYADMVVGSEEDNAILFHEKINPYSVKLYVPCPPNARATLKVDVIFEEYVGLDIHKKHVVSREDFHVTKVFGIKKAEPLYNLPAESCLVSSKTTRTNQSKYHNGQNPLSKEVCVIEDDFRAKAPDKDDNDLEILGTREYNNLASLEAPSFTLLHEEDYEDVPDVLASEPVEAECKSATNNTIFDHIRKKSRDFPNLMLSKSMDSSYEPLILKKMKTSGDQFGLDQSSLHAYEVTPMVFDRTEARVSPNNTDERCRNILTRTAETRSFQFTGKMDSISQKSEILNRTQGKNSTQLAGKKAFEKSKTPDENSLHFVGKRDSSSEKSKALSKTPDENSLQFLGKMDSSSEKSKFCFSSPLADFQPMQCTKQVAASVQPLTIQDYCKDILASAKSSGTGASFLDVKSVFSFL*