Gene Details:

• MSU gene ID: LOC_Os02g40030
• RAPdb gene ID: Os02g0614100
• Gene Symbol: OsNST1 BC14
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Map-based cloning revealed that all of the observed mutant phenotypes result from a missense mutation in a putative NST gene, Oryza sativa Nucleotide Sugar Transporter1 (OsNST1).
• OsNST1 was identified as a Golgi-localized transporter by analysis of a fluorescence-tagged OsNST1 expressed in rice protoplast cells and demonstration of UDP-glucose transport activity via uptake assays in yeast.
• The inferior mechanical strength and abnormal development of bc14 plants suggest that OsNST1 has pleiotropic effects on cell wall biosynthesis and plant growth.
• Compositional sugar analyses in total and fractionated wall residues of wild-type and bc14 culms showed a deficiency in the synthesis of glucoconjugated polysaccharides in bc14, indicating that OsNST1 supplies the glucosyl substrate for the formation of matrix polysaccharides, and thereby modulates cellulose biosynthesis.
• A previously undescribed Golgi NST mutant, brittle culm14 (BC14), displays reduced mechanical strength caused by decreased cellulose content and altered wall structure, and exhibits abnormalities in plant development.
• Identification of OsNST1 has improved our understanding of how cell wall polysaccharide synthesis is regulated by Golgi NSTs in plants.
• We previously examined cell wall composition of BC14 and wild type plants.

Function-related keywords:

• transporter ,  growth ,  cell-wall ,  cellulose ,  culm

Literature:

• Golgi nucleotide sugar transporter modulates cell wall biosynthesis and plant growth in rice .  DOI:  10.1073/pnas.1016144108 ;  PMID:  21383162
• Golgi-localized UDP-glucose transporter is required for cell wall integrity in rice .  DOI:  10.4161/psb.6.8.16379 ;  PMID:  21822061

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: