Gene Details:

• MSU gene ID: LOC_Os02g39390
• RAPdb gene ID: Os02g0606700
• Gene Symbol: RSS1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• No correlation between sucrose synthase activity and starch biosynthesis was seen in these mutants, although slight elevations of RSS1 transcript levels were observed.
• We previously showed that the rice protein RSS1, whose stability is regulated depending on the cell cycle phases, is a key factor for the maintenance of meristematic activity under stressful conditions.
• Molecular characterization of the rice protein RSS1 required for meristematic activity under stressful conditions.
• RSS1 was expressed predominantly in the endosperm of milky stage rice seeds with maximum expression at 35 days after pollination which were 810-fold over leaf levels.
• Cloning and expression of rice (Oryza sativa) sucrose synthase 1 (RSS1) in developing seed endosperm.
• Here we show that RSS1 interacts with all the PP1 expressed in the shoot apex of rice.
• These effects of RSS1 are exerted by regulating the G1-S transition, possibly through an interaction of RSS1 with protein phosphatase 1, and are mediated by the phytohormone, cytokinin.
• ) sucrose synthase 1 (RSS1) was used to determine spatial expression of the gene in rice tissues and temporal expression in developing rice endosperm.
• Here we show that a rice protein, RSS1, whose stability is controlled by cell cycle phases, contributes to the vigour of meristematic cells and viability under salinity conditions.
• RSS1 regulates the cell cycle and maintains meristematic activity under stress conditions in rice.
• Interestingly, the recombinant RSS1 protein is highly resistant to heat with respect to its anti-coagulability and binding activity to PP1.
• RSS1 transcript and activity levels were analyzed in two starch deficient mutants of rice to determine if the lesion in these mutants resides at the locus for RSS1.
• The wheat TdRL1 is the functional homolog of the rice RSS1 and promotes plant salt stress tolerance.
• Finally, when expressed in the rice RSS1 mutant, TdRL1 suppressed its dwarf phenotype upon salt stress, confirming that both proteins are true functional homologs required for salt stress tolerance in cereals.
• To further understand their function, we performed a computational analysis to compare RSS1 and TdRL1 co-expression networks revealing common gene ontologies, among which those related to cell cycle progression and regulation of microtubule (MT) networks were over-represented.

Function-related keywords:

• starch-biosynthesis ,  meristem ,  seed ,  shoot ,  phytohormone ,  cytokinin ,  endosperm ,  salinity ,  resistant ,  starch ,  cell-cycle ,  leaf ,  salt ,  tolerance ,  salt-stress ,  stress ,  dwarf ,  stress-tolerance

Literature:

• Cloning and expression of rice (Oryza sativa) sucrose synthase 1 (RSs1) in developing seed endosperm .  DOI:  10.1016/0168-9452(95)04288-1
• Molecular characterization of the rice protein RSS1 required for meristematic activity under stressful conditions .  DOI:  10.1016/j.plaphy.2012.09.006 ;  PMID:  23041461
• RSS1 regulates the cell cycle and maintains meristematic activity under stress conditions in rice .  DOI:  10.1038/ncomms1279 ;  PMID:  21505434
• Maintenance of meristem activity under stress: is there an interplay of RSS1-like proteins with the RBR pathway? .  DOI:  10.1111/plb.12424 ;  PMID:  26663822
• The wheat TdRL1 is the functional homolog of the rice RSS1 and promotes plant salt stress tolerance .  DOI:  10.1007/s00299-018-2333-2 ;  PMID:  30099611

Related News:

Gene Resources:

• NCBI ID: AK063512
• UniProt accessions:

Sequences: