Gene Details:

• MSU gene ID: LOC_Os02g36974
• RAPdb gene ID: Os02g0580300
• Gene Symbol: OsGF14e GF14e GID2
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Release of the repressive activity of rice DELLA protein SLR1 by gibberellin does not require SLR1 degradation in the GID2 mutant.
• In GID2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form and GA increased its concentration, whereas SLR1 was rapidly degraded by GA through ubiquitination in the wild type.
• We conclude that GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCF(GID2)-proteasome pathway.
• Enhanced resistance was correlated with GF14e silencing prior to and after development of the LM phenotype, higher basal expression of a defense response peroxidase gene (POX22.
• Together, our findings suggest that GF14e negatively affects the induction of plant defense response genes, cell death and broad-spectrum resistance in rice.
• Disturbing GA homeostasis affected the expression of the GA signaling genes GID1 (GIBBERELLIN INSENSITIVE DWARF 1), GID2 and SLR1.
• We isolated and characterized a rice GA-insensitive dwarf mutant, GID2.
• It is assumed that interaction between GIBBERELLIN INSENSITIVE DWARF1 (GID1) and the N-terminal DELLA/TVHYNP motif of SLR1 triggers F-box protein GID2-mediated SLR1 degradation.
• To determine whether the GF14e gene plays a direct role in resistance to disease in rice, we suppressed its expression by RNAi silencing.
• Rice 14-3-3 protein (GF14e) negatively affects cell death and disease resistance.
• GF14e-silenced rice plants showed high levels of resistance to a virulent strain of Xoo compared with plants that were not silenced.
• GA perception by GID1 causes SLR1 protein degradation involving the F-box protein GID2; this triggers GA-associated responses such as shoot elongation and seed germination.
• Previously, we identified a rice F-box protein, gibberellin-insensitive dwarf2 (GID2), which is essential for GA-mediated DELLA protein degradation.
• GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice.
• The level of SLR1 protein in GID2 was decreased by loss of GID1 function or treatment with a GA biosynthesis inhibitor, and dwarfism was enhanced.
• These results indicate that derepression of SLR1 repressive activity can be accomplished by GA and GID1 alone and does not require F-box (GID2) function.
• Evidence for GA signaling without GID2 was also provided by the expression behavior of GA-regulated genes such as GA-20oxidase1, GID1, and SLR1 in the GID2 mutant.
• An exception is the GA-insensitive F-box mutant GID2, which shows milder dwarfism than mutants such as gid1 and cps even though it accumulates higher levels of SLR1.
• Conversely, overproduction of GID1 or treatment with GA(3) increased the SLR1 level in GID2 and reduced dwarfism.
• OsGF14e positively regulates panicle blast resistance in rice.
• Rice black streaked dwarf virus P7-2 forms a SCF complex through binding to Oryza sativa SKP1-like proteins, and interacts with GID2 involved in the gibberellin pathway.
• Overall, these results indicated that P7-2 functioned as a component of the SCF complex in rice, and interaction of P7-2 with GID2 implied possible roles of the GA signaling pathway during RBSDV infection.

Function-related keywords:

• gibberellin ,  ga ,  defense ,  dwarf ,  disease ,  defense-response ,  disease-resistance ,  xoo ,  shoot ,  cell-death ,  seed-germination ,  seed ,  homeostasis ,  resistance ,  panicle ,  blast ,  blast-resistance ,  Gibberellin ,  GA

Literature:

• Characterization of the molecular mechanism underlying gibberellin perception complex formation in rice .  DOI:  10.1105/tpc.110.075549 ;  PMID:  20716699
• The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress .  DOI:  10.1093/dnares/dsl001 ;  PMID:  16766513
• Gibberellin homeostasis and plant height control by EUI and a role for gibberellin in root gravity responses in rice .  DOI:  10.1038/cr.2008.28 ;  PMID:  18268540
• GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice .  DOI:  10.1111/j.1365-313x.2003.01990.x ;  PMID:  14756772
• Rice 14-3-3 protein (GF14e) negatively affects cell death and disease resistance .  DOI:  10.1111/j.1365-313X.2011.04728.x ;  PMID:  21793954
• OsGRF6 interacts with SLR1 to regulate OsGA2ox1 expression for coordinating chilling tolerance and growth in rice .  DOI:  10.1016/j.jplph.2021.153406 ;  PMID:  33756268
• Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant .  DOI:  10.1126/science.1081077 ;  PMID:  12649483
• OsGF14e positively regulates panicle blast resistance in rice .  DOI:  10.1016/j.bbrc.2016.02.005 ;  PMID:  26851365
• Rice black streaked dwarf virus P7-2 forms a SCF complex through binding to Oryza sativa SKP1-like proteins, and interacts with GID2 involved in the gibberellin pathway .  DOI:  10.1371/journal.pone.0177518 ;  PMID:  28494021

Related News:

Gene Resources:

• NCBI ID: AB100246
• UniProt accessions:

Sequences: