Information report for OsCPS2;OsCyc2
Gene Details
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Functional Descriptions
- Several enzymatic properties of OsCPS2/OsCyc2, including the optimal pH, optimal temperature, divalent cation requirement, and kinetic values for the geranylgeranyl diphosphate (GGDP) substrate, were almost the same as those of OsCPS1.
- Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1.
- The rice genome contains two ent-copalyl diphosphate synthase genes: OsCPS1 acts in gibberellin (phytohormone) biosynthesis, and OsCPS2/OsCyc2 acts in the synthesis of oryzalexins A-F and phytocassanes A-E (phytoalexins).
- Furthermore, the OsCPS1 activity exhibited approximately 70% inhibition by 100 muM Amo-1618 (a gibberellin biosynthetic inhibitor), whereas the OsCPS2/OsCyc2 activity exhibited approximately 10% inhibition.
- The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice.
- Rice (Oryza sativa L.) has two ent-CDP synthase genes, OsCPS1 and OsCPS2.
- The results indicate that transcripts of the two ent-CDP synthase genes differentially localize in rice plants according to their distinct biological roles, OsCPS1 for growth and OsCPS2 for defence.
- It has been thought that OsCPS1 participates in GA biosynthesis, while OsCPS2 participates in the biosynthesis of phytoalexins, phytocassanes A-E, and oryzalexins A-F.
Functional Keywords
Literature and News
- Comparison of the enzymatic properties of ent-copalyl diphosphate synthases in the biosynthesis of phytoalexins and gibberellins in rice . DOI: 10.1271/bbb.70615 ; PMID: 18256497
- Cloning and characterization of cDNAs encoding ent-copalyl diphosphate synthases in wheat: insight into the evolution of rice phytoalexin biosynthetic genes . DOI: 10.1271/bbb.80781 ; PMID: 19270400
- gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein (PBZ1) in response to cold stress and pathogen attack . DOI: 10.1111/j.1365-3040.2005.01441.x ; PMID: 17080612
- Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis . DOI: 10.1111/j.1365-313X.2004.02175.x ; PMID: 15341631
- Transcripts of two ent-copalyl diphosphate synthase genes differentially localize in rice plants according to their distinct biological roles . DOI: 10.1093/jxb/eru424 ; PMID: 25336684
- Inferring Roles in Defense from Metabolic Allocation of Rice Diterpenoids . DOI: 10.1105/tpc.18.00205 ; PMID: 29691314
- A (conditional) role for labdane-related diterpenoid natural products in rice stomatal closure . DOI: 10.1111/nph.17196 ; PMID: 33458815
Gene Resources
- UniProt: C7IY39
- EMBL: AP008208
- AlphaFoldDB: C7IY39
- EnsemblPlants: Os02t0571100-01
- Gramene: Os02t0571100-01
- KEGG: dosa:Os02g0571100
- InterPro: IPR001906, IPR008930, IPR008949
- PANTHER: PTHR31739, PTHR31739:SF4
- SUPFAM: SSF48239, SSF48576
- Gene3D: 1.10.600.10, 1.50.10.130, 1.50.10.160
- OrthoDB: C7IY39
- SWISS-MODEL: C7IY39
Sequences
cDNA Sequence
- >LOC_Os02g36210.1
GAGCTACTACGCCGAGACAGTGTGGTGGTGAGCCGGTGATCGATCTTGCATCTTGCGAGCACACGGGATTCCAGATCGATCGAGCTATAGCTAGCTAGCTAATTAGTTACCACGTACGAACGAGTACTTTACAACTCGATCAGTTCATCAAATTAACTCGATCAGTGGTTAAGTTTCGCACGACACACGCGCGCGGATCGGAAGCCATCATGCAGATGCAGGTGCTCACCGCTGCTTCTTCGCTCCCTCGCGCGACCTTGCTCCGGCCGGCGGCTGCCGAGCCATGGCGCCAATCTTTCCTGCAGCTGCAGGCTCGTCCAATCCAGCGACCAGGTATCATGCTACACTGCAAGGCCCAGCTACAGGGGCAGGAAACGCGCGAGCGTCGTCAGCTCGACGACGATGAACACGCTAGACCACCACAGGGCGGCGACGACGACGTCGCAGCAAGCACCAGCGAGCTACCCTACATGATCGAGTCCATCAAATCCAAGCTGAGGGCGGCCAGGAACAGCCTCGGCGAGACCACCGTCTCCGCCTACGACACGGCGTGGATCGCGCTCGTCAACCGCCTCGACGGCGGCGGCGAGAGGAGCCCCCAGTTCCCGGAGGCCATCGACTGGATCGCCCGGAACCAGCTGCCCGACGGCTCGTGGGGCGACGCCGGCATGTTCATCGTCCAGGACCGGCTCATCAACACGCTGGGCTGCGTCGTGGCGCTCGCGACGTGGGGCGTCCACGAGGAGCAGCGCGCGAGGGGCCTCGCCTACATCCAGGACAACCTCTGGAGGCTCGGCGAGGACGACGAGGAGTGGATGATGGTCGGGTTCGAGATCACCTTCCCCGTTCTCCTCGAGAAGGCCAAGAACCTGGGCCTGGACATCAACTATGATGACCCTGCCTTGCAGGACATATATGCCAAGAGACAATTAAAGCTCGCAAAGATTCCTAGAGAAGCACTGCATGCTAGGCCGACCACCTTGCTCCATAGCTTAGAGGGAATGGAAAACTTGGACTGGGAAAGGTTGCTACAGTTCAAGTGTCCAGCTGGCTCCTTACATTCCTCACCTGCTGCGTCAGCTTACGCTCTCAGCGAAACAGGTGACAAGGAGTTGCTCGAATACCTGGAAACAGCCATCAACAATTTTGACGGTGGAGCACCATGCACCTACCCTGTCGACAACTTTGACCGCTTATGGTCGGTCGATCGGTTGAGGCGGCTAGGAATATCGAGGTACTTCACGAGTGAGATTGAAGAATACTTGGAGTACGCCTACAGGCACCTGAGTCCAGATGGCATGAGCTACGGCGGGCTCTGTCCGGTCAAGGACATCGACGACACGGCCATGGCTTTCCGTCTCCTCCGTCTGCATGGCTACAATGTCTCATCATCGGTGTTCAATCACTTCGAGAAGGACGGGGAGTACTTCTGCTTCGCGGGGCAGTCGAGCCAGTCGCTGACGGCGATGTACAACTCCTACCGCGCCTCGCAGATCGTCTTCCCCGGCGACGACGACGGCCTGGAGCAGCTCAGGGCCTACTGCCGCGCCTTCCTCGAGGAGCGGCGAGCCACCGGCAACCTCATGGACAAGTGGGTCATCGCCAATGGCTTGCCCAGCGAGGTCGAGTACGCGCTGGATTTCCCATGGAAGGCAAGCTTGCCGCGAGTCGAGACGAGGGTGTATCTGGAGCAGTACGGCGCTAGCGAGGACGCGTGGATCGGCAAGGGACTCTACAGGATGACCCTAGTCAACAACGACCTGTACCTTGAGGCGGCAAAGGCTGACTTCACCAACTTCCAGAGGCTCTCCCGGCTCGAGTGGCTCAGCCTGAAAAGGTGGTACATCAGGAACAATCTGCAAGCGCACGGCGTGACCGAACAGAGCGTGCTGAGAGCCTACTTCTTAGCCGCGGCGAACATCTTCGAGCCCAACCGGGCGGCGGAACGCCTGGGATGGGCTCGCACGGCGATCCTCGCCGAGGCCATCGCGTCACACCTCCGACAGTACAGTGCCAACGGCGCCGCCGACGGCATGACAGAGAGGCTCATCAGTGGACTCGCCAGCCACGACTGGGACTGGAGGGAATCAAAGGATTCAGCAGCGAGGAGCTTACTGTACGCACTTGATGAGCTCATCGACCTCCATGCGTTCGGCAATGCTTCTGACAGCCTACGTGAAGCGTGGAAGCAGTGGCTCATGTCATGGACAAACGAGAGCCAAGGATCAACTGGTGGGGATACCGCATTGCTGCTAGTTCGCACAATCGAGATCTGCTCAGGACGGCACGGTTCAGCCGAGCAGAGCCTGAAGAACAGTGCAGACTACGCCAGGCTTGAGCAGATCGCCTCTTCCATGTGCAGCAAACTTGCCACCAAAATTCTTGCTCAGAATGGAGGAAGCATGGACAACGTTGAGGGTATAGACCAGGAAGTGGATGTTGAGATGAAAGAGCTGATCCAGCGTGTCTACGGGAGCAGCAGCAACGATGTCAGCAGCGTGACGAGGCAGACATTTCTCGACGTGGTGAAGAGCTTTTGCTACGTCGCTCATTGCTCGCCCGAAACAATCGATGGGCACATCTCCAAGGTTTTGTTCGAGGATGTCAATTAGGAAAATGGTTGACTACTAGAAAGGCAATAAAAACAAGAGTTGTGTCTACCAGCATGGCTCGTAAAGTGATGACTGCGAAAATTTTCTCAAAGTGAGATGAATTTAGTCGATATTTGTTTCACTTGATGGTACACCAGATACACATGTTGTTATAAGTTGTCTGATAGCTAGTATACTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGAATATGTTCTGAAGTGAGACGTCAGCTATATGAATTGTCTTATTTCAGAACAAAACTTTGCACATCGATATGCCATTAAAAATAAATATGTAAATATGTGAACCTTTTTTTCTTTGTGCTCTTTCTTTTGTTTCCCATCTCAATGACTACGAGTTCTAGATCTTCTTTGCAAATAGAAGAACTTTGC
CDS Sequence
- >LOC_Os02g36210.1
ATGCAGATGCAGGTGCTCACCGCTGCTTCTTCGCTCCCTCGCGCGACCTTGCTCCGGCCGGCGGCTGCCGAGCCATGGCGCCAATCTTTCCTGCAGCTGCAGGCTCGTCCAATCCAGCGACCAGGTATCATGCTACACTGCAAGGCCCAGCTACAGGGGCAGGAAACGCGCGAGCGTCGTCAGCTCGACGACGATGAACACGCTAGACCACCACAGGGCGGCGACGACGACGTCGCAGCAAGCACCAGCGAGCTACCCTACATGATCGAGTCCATCAAATCCAAGCTGAGGGCGGCCAGGAACAGCCTCGGCGAGACCACCGTCTCCGCCTACGACACGGCGTGGATCGCGCTCGTCAACCGCCTCGACGGCGGCGGCGAGAGGAGCCCCCAGTTCCCGGAGGCCATCGACTGGATCGCCCGGAACCAGCTGCCCGACGGCTCGTGGGGCGACGCCGGCATGTTCATCGTCCAGGACCGGCTCATCAACACGCTGGGCTGCGTCGTGGCGCTCGCGACGTGGGGCGTCCACGAGGAGCAGCGCGCGAGGGGCCTCGCCTACATCCAGGACAACCTCTGGAGGCTCGGCGAGGACGACGAGGAGTGGATGATGGTCGGGTTCGAGATCACCTTCCCCGTTCTCCTCGAGAAGGCCAAGAACCTGGGCCTGGACATCAACTATGATGACCCTGCCTTGCAGGACATATATGCCAAGAGACAATTAAAGCTCGCAAAGATTCCTAGAGAAGCACTGCATGCTAGGCCGACCACCTTGCTCCATAGCTTAGAGGGAATGGAAAACTTGGACTGGGAAAGGTTGCTACAGTTCAAGTGTCCAGCTGGCTCCTTACATTCCTCACCTGCTGCGTCAGCTTACGCTCTCAGCGAAACAGGTGACAAGGAGTTGCTCGAATACCTGGAAACAGCCATCAACAATTTTGACGGTGGAGCACCATGCACCTACCCTGTCGACAACTTTGACCGCTTATGGTCGGTCGATCGGTTGAGGCGGCTAGGAATATCGAGGTACTTCACGAGTGAGATTGAAGAATACTTGGAGTACGCCTACAGGCACCTGAGTCCAGATGGCATGAGCTACGGCGGGCTCTGTCCGGTCAAGGACATCGACGACACGGCCATGGCTTTCCGTCTCCTCCGTCTGCATGGCTACAATGTCTCATCATCGGTGTTCAATCACTTCGAGAAGGACGGGGAGTACTTCTGCTTCGCGGGGCAGTCGAGCCAGTCGCTGACGGCGATGTACAACTCCTACCGCGCCTCGCAGATCGTCTTCCCCGGCGACGACGACGGCCTGGAGCAGCTCAGGGCCTACTGCCGCGCCTTCCTCGAGGAGCGGCGAGCCACCGGCAACCTCATGGACAAGTGGGTCATCGCCAATGGCTTGCCCAGCGAGGTCGAGTACGCGCTGGATTTCCCATGGAAGGCAAGCTTGCCGCGAGTCGAGACGAGGGTGTATCTGGAGCAGTACGGCGCTAGCGAGGACGCGTGGATCGGCAAGGGACTCTACAGGATGACCCTAGTCAACAACGACCTGTACCTTGAGGCGGCAAAGGCTGACTTCACCAACTTCCAGAGGCTCTCCCGGCTCGAGTGGCTCAGCCTGAAAAGGTGGTACATCAGGAACAATCTGCAAGCGCACGGCGTGACCGAACAGAGCGTGCTGAGAGCCTACTTCTTAGCCGCGGCGAACATCTTCGAGCCCAACCGGGCGGCGGAACGCCTGGGATGGGCTCGCACGGCGATCCTCGCCGAGGCCATCGCGTCACACCTCCGACAGTACAGTGCCAACGGCGCCGCCGACGGCATGACAGAGAGGCTCATCAGTGGACTCGCCAGCCACGACTGGGACTGGAGGGAATCAAAGGATTCAGCAGCGAGGAGCTTACTGTACGCACTTGATGAGCTCATCGACCTCCATGCGTTCGGCAATGCTTCTGACAGCCTACGTGAAGCGTGGAAGCAGTGGCTCATGTCATGGACAAACGAGAGCCAAGGATCAACTGGTGGGGATACCGCATTGCTGCTAGTTCGCACAATCGAGATCTGCTCAGGACGGCACGGTTCAGCCGAGCAGAGCCTGAAGAACAGTGCAGACTACGCCAGGCTTGAGCAGATCGCCTCTTCCATGTGCAGCAAACTTGCCACCAAAATTCTTGCTCAGAATGGAGGAAGCATGGACAACGTTGAGGGTATAGACCAGGAAGTGGATGTTGAGATGAAAGAGCTGATCCAGCGTGTCTACGGGAGCAGCAGCAACGATGTCAGCAGCGTGACGAGGCAGACATTTCTCGACGTGGTGAAGAGCTTTTGCTACGTCGCTCATTGCTCGCCCGAAACAATCGATGGGCACATCTCCAAGGTTTTGTTCGAGGATGTCAATTAG
Protein Sequence
- >LOC_Os02g36210.1
MQMQVLTAASSLPRATLLRPAAAEPWRQSFLQLQARPIQRPGIMLHCKAQLQGQETRERRQLDDDEHARPPQGGDDDVAASTSELPYMIESIKSKLRAARNSLGETTVSAYDTAWIALVNRLDGGGERSPQFPEAIDWIARNQLPDGSWGDAGMFIVQDRLINTLGCVVALATWGVHEEQRARGLAYIQDNLWRLGEDDEEWMMVGFEITFPVLLEKAKNLGLDINYDDPALQDIYAKRQLKLAKIPREALHARPTTLLHSLEGMENLDWERLLQFKCPAGSLHSSPAASAYALSETGDKELLEYLETAINNFDGGAPCTYPVDNFDRLWSVDRLRRLGISRYFTSEIEEYLEYAYRHLSPDGMSYGGLCPVKDIDDTAMAFRLLRLHGYNVSSSVFNHFEKDGEYFCFAGQSSQSLTAMYNSYRASQIVFPGDDDGLEQLRAYCRAFLEERRATGNLMDKWVIANGLPSEVEYALDFPWKASLPRVETRVYLEQYGASEDAWIGKGLYRMTLVNNDLYLEAAKADFTNFQRLSRLEWLSLKRWYIRNNLQAHGVTEQSVLRAYFLAAANIFEPNRAAERLGWARTAILAEAIASHLRQYSANGAADGMTERLISGLASHDWDWRESKDSAARSLLYALDELIDLHAFGNASDSLREAWKQWLMSWTNESQGSTGGDTALLLVRTIEICSGRHGSAEQSLKNSADYARLEQIASSMCSKLATKILAQNGGSMDNVEGIDQEVDVEMKELIQRVYGSSSNDVSSVTRQTFLDVVKSFCYVAHCSPETIDGHISKVLFEDVN*