Information report for OsAPx8
Gene Details
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Functional Descriptions
- Here we show that ABA level but not OsAPx8 expression was enhanced at a concentration of mannitol iso-osmotic with 150 mM NaCl suggests that NaCl-enhanced OsAPx8 expression is not associated with osmotic component.
- NaCl at 150 mM and 200 mM increased the expression of OsAPx8 and the activities of APx, but had no effect on the expression of OsAPx1, OsAPx2, OsAPx3, OsAPx4, OsAPx5, OsAPx6, and OsAPx7 in rice roots.
- Exogenously applied ABA also specifically enhanced the expression of OsAPx8 in rice roots.
- It appears that NaCl-enhanced expression of OsAPx8 in rice roots is mediated through an accumulation of ABA.
- Evidence is provided to show that Na(+) but not Cl(-) is required for enhancing OsAPx8 expression, APx activity, and ABA accumulation in rice roots treated with NaCl.
- Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H(2)O(2) in rice roots, did not suppress OsAPx8 induction and ABA accumulation by NaCl.
- It appears that H(2)O(2) is not involved in the regulation of NaCl-induced OsAPx8 expression in rice roots.
- Applying semi-quantitative RT-PCR, the mRNA levels were quantified for two cytosolic (OsAPx1 and OsAPx2), two peroxisomal (OsAPx3 and OsAPx4), and four chloroplastic (OsAPx5, OsAPx6, OsAPx7, and OsAPx8) isoforms identified in the rice genome.
- Eight types of APx have been described for Oryza sativa: two cytosolic (OsAPx1 and OsAPx2), two putative peroxisomal (OsAPx3 and OsAPx4), and four chloroplastic isoforms (OsAPx5, OsAPx6, OsAPx7, and OsAPx8).
- We have recently demonstrated that Na+ but not Cl- is required for the NaCl-induced expression of OsAPx8 in rice roots.
- The rice thylakoid membrane-bound ascorbate peroxidase OsAPx8 functions in tolerance to bacterial blight.
- In addition, OsAPx8 interacted with the susceptibility protein Os8N3/Xa13, and their binding repressed the reaction of OsAPx8 in tolerance to bacterial blight.
Functional Keywords
- ABA , root , chloroplast , tolerance , blight , bacterial-blight
Literature and News
- Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings . DOI: 10.1016/j.jplph.2011.11.012 ; PMID: 22196946
- Expression of ASCORBATE PEROXIDASE 8 in roots of rice (Oryza sativa L.) seedlings in response to NaCl . DOI: 10.1093/jxb/erm174 ; PMID: 17916638
- Expression of ASCORBATE PEROXIDASE 8 in roots of rice (Oryza sativa L.) seedlings in response to NaCl . DOI: 10.1093/jxb/erm174 ; PMID: 17916638
- The rice thylakoid membrane-bound ascorbate peroxidase OsAPX8 functions in tolerance to bacterial blight . DOI: 10.1038/srep26104 ; PMID: 27185545
- Proteomic and physiological approaches reveal new insights for uncover the role of rice thylakoidal APX in response to drought stress . DOI: 10.1016/j.jprot.2018.08.014 ; PMID: 30170113
Gene Resources
- UniProt: Q69SV0
- EMBL: AB114856, AP004876, AP008208
- AlphaFoldDB: Q69SV0
- EnsemblPlants: Os02t0553200-01
- Gramene: Os02t0553200-01
- KEGG: dosa:Os02g0553200
- InterPro: IPR002016, IPR002207, IPR010255
- PANTHER: PTHR31356, PTHR31356:SF11
- SUPFAM: SSF48113
- PROSITE: PS00435, PS50873
- Gene3D: 1.10.420.10, 1.10.520.10
- OrthoDB: Q69SV0
- SWISS-MODEL: Q69SV0
- Conserved Domain Database: cd00691
- eggNOG: ENOG502QS7Q
Sequences
cDNA Sequence
- >LOC_Os02g34810.1
GAAAAAACTCACTCGACTCGAGCGCGCGCGCCATGGCGGAGCGCATCGCCGCCTCCCTCCTCCCGGCTGCCTCGCCCTCGCCTGCTCCGTCCCCTCCCCCCCCGCGCCCCCGCGTCTCCGCCGCGGCCGCCGCCTCCTTCCCATGCTGCTCCACCAGCGCCGGCGGCCTCCGCCTCCGCTCCCGCCCGTCTCGCTTCCCGCAGAAGGCTGCGACGACGAGGAGCGGGCGCGCCGGCGCGGGGGCGCGGGCGGTGGTCCGGTGCATGGCGGCGGCGGCGGTGGCGGCGTCCGACGCGGCGCAGCTCAAGAGCGCCCGGGAGGACATCAGGGAGATCCTCAAGACCACCTACTGCCACCCCATCATGGTCCGTCTTGGGTGGCACGATTCTGGCACGTACGACAAGAACATCGAGGAGTGGCCGCAGAGGGGCGGAGCCGACGGGAGCTTGAGATTTGACGCCGAATTGAGCCACGGAGCCAATGCTGGTCTGATTAATGCTTTGAAGCTTATCCAACCAATCAAGGACAAATACCCGGGTATAACTTATGCTGATTTGTTCCAGTTGGCAAGTGCTACAGCAATTGAGGAAGCTGGTGGGCCGAAAATTCCAATGAAATATGGACGAGTTGATGTCACAGCAGCTGAGCAGTGCCCACCAGAGGGGAGGCTTCCTGATGCCGGTCCACGTGTGCCCGCTGATCATCTTAGGGAGGTATTCTACAGGATGGGCCTTGATGACAAGGAAATTGTTGCATTATCTGGAGCACACACACTTGGAAGATCAAGACCTGACAGGAGTGGCTGGGGAAAGCCAGAAACAAAATATACTAAGGATGGGCCTGGTGAACCTGGAGGGCAATCATGGACAGTTGAATGGTTGAAGTTTGATAACAGTTACTTCAAGGACATAAAAGAGCAAAGGGACCAGGATCTTCTAGTGCTACCCACAGATGCTGCATTATTTGAGGATCCGTCCTTCAAGGTATATGCCGAAAAATATGCAGAGGATCAGGAGGCATTCTTTAAAGACTACGCTGAAGCTCATGCTAAACTGAGCGACCTTGGTGCAAAGTTCGATCCACCTGAGGGATTTTCACTGGACGATGAACCAGCCGTCGAAGAGAAGGATCCTGAACCAGCACCAGCGCCAGCAGCAGCACCACCACCTCCACCAGTCGAGGAGAAGAAGGAAGCTGAACCAACTCCAGTACCAGTAACGGTAGGAGCAGCAGTGGCATCATCGCCAGCGGATGACAACAACGGTGCAGCACCGCAACCAGAGCCCTTCGTCGCTGCGAAATACTCCTACGGAAAGAAGGAGCTGTCGGACTCGATGAAGCAGAAGATCAGGGCGGAGTACGAGGGATTCGGAGGCAGCCCGGACAAGCCTCTGCAGTCCAACTACTTCCTCAACATCATGCTCTTGATCGGAGGGCTGGCCTTCTTGACGTCTCTGCTCGGGAGCTGAGAGCGATGGTCTGATGACCTCCTCTGACGAGTGTTTTGAGTTGTTCTGCCTGTGCTAAGATTTGCGTGTTTCTCTTTCCATGTTTTGAGTCGTTATTCCGTAAATAAATTGAGGTAAAAGGATGGGCATGTGAATGGATTCCAGTACTTTCTGAACTTCTTGAGTAATTCCTCTAGAGAAATATTTGTCGGAGAGAAATGTCACATTATCTAGTTGTCATGGCTCATGACTACATTCTCTATACTGAGGGAAATTATGACATTCTCTAAACTAAGGAGGGAAATAATGAGGACACCCCATATATCACTCTTTCTTATGGCATGTTCAGTATAAAAGGAAAGTGCATTTTCCTCTCC
CDS Sequence
- >LOC_Os02g34810.1
ATGGCGGAGCGCATCGCCGCCTCCCTCCTCCCGGCTGCCTCGCCCTCGCCTGCTCCGTCCCCTCCCCCCCCGCGCCCCCGCGTCTCCGCCGCGGCCGCCGCCTCCTTCCCATGCTGCTCCACCAGCGCCGGCGGCCTCCGCCTCCGCTCCCGCCCGTCTCGCTTCCCGCAGAAGGCTGCGACGACGAGGAGCGGGCGCGCCGGCGCGGGGGCGCGGGCGGTGGTCCGGTGCATGGCGGCGGCGGCGGTGGCGGCGTCCGACGCGGCGCAGCTCAAGAGCGCCCGGGAGGACATCAGGGAGATCCTCAAGACCACCTACTGCCACCCCATCATGGTCCGTCTTGGGTGGCACGATTCTGGCACGTACGACAAGAACATCGAGGAGTGGCCGCAGAGGGGCGGAGCCGACGGGAGCTTGAGATTTGACGCCGAATTGAGCCACGGAGCCAATGCTGGTCTGATTAATGCTTTGAAGCTTATCCAACCAATCAAGGACAAATACCCGGGTATAACTTATGCTGATTTGTTCCAGTTGGCAAGTGCTACAGCAATTGAGGAAGCTGGTGGGCCGAAAATTCCAATGAAATATGGACGAGTTGATGTCACAGCAGCTGAGCAGTGCCCACCAGAGGGGAGGCTTCCTGATGCCGGTCCACGTGTGCCCGCTGATCATCTTAGGGAGGTATTCTACAGGATGGGCCTTGATGACAAGGAAATTGTTGCATTATCTGGAGCACACACACTTGGAAGATCAAGACCTGACAGGAGTGGCTGGGGAAAGCCAGAAACAAAATATACTAAGGATGGGCCTGGTGAACCTGGAGGGCAATCATGGACAGTTGAATGGTTGAAGTTTGATAACAGTTACTTCAAGGACATAAAAGAGCAAAGGGACCAGGATCTTCTAGTGCTACCCACAGATGCTGCATTATTTGAGGATCCGTCCTTCAAGGTATATGCCGAAAAATATGCAGAGGATCAGGAGGCATTCTTTAAAGACTACGCTGAAGCTCATGCTAAACTGAGCGACCTTGGTGCAAAGTTCGATCCACCTGAGGGATTTTCACTGGACGATGAACCAGCCGTCGAAGAGAAGGATCCTGAACCAGCACCAGCGCCAGCAGCAGCACCACCACCTCCACCAGTCGAGGAGAAGAAGGAAGCTGAACCAACTCCAGTACCAGTAACGGTAGGAGCAGCAGTGGCATCATCGCCAGCGGATGACAACAACGGTGCAGCACCGCAACCAGAGCCCTTCGTCGCTGCGAAATACTCCTACGGAAAGAAGGAGCTGTCGGACTCGATGAAGCAGAAGATCAGGGCGGAGTACGAGGGATTCGGAGGCAGCCCGGACAAGCCTCTGCAGTCCAACTACTTCCTCAACATCATGCTCTTGATCGGAGGGCTGGCCTTCTTGACGTCTCTGCTCGGGAGCTGA
Protein Sequence
- >LOC_Os02g34810.1
MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAGGLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREILKTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLIQPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGPRVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQSWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTPVPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSPDKPLQSNYFLNIMLLIGGLAFLTSLLGS*