Gene Details:
- MSU gene ID: LOC_Os02g34600
- RAPdb gene ID: Os02g0551100
- Gene Symbol: SAPK6 OSRK1 OsSAPK6
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- The OSRK1 transcript level was undetectable in vegetative tissues, but significantly increased by hyperosmotic stress and Abscisic acid (ABA).
- Finally, ectopic expression of OSRK1 in transgenic tobacco resulted in a reduced sensitivity to ABA in seed germination and root elongation.
- Further studies demonstrated that overexpression of OsMYB48-1 could regulate the expression of some ABA biosynthesis genes (OsNCED4, OsNCED5), early signaling genes (OsPP2C68, OSRK1) and late responsive genes (RAB21, OsLEA3, RAB16C and RAB16D) under drought stress conditions.
- These findings suggest that OSRK1 is associated with ABA signaling, possibly through the phosphorylation of ABF family in vivo.
- Interestingly, OSRK1 showed strong substrate preference for rice bZIP transcription factors and uncommon cofactor requirement for Mn(2+) over Mg(2+).
- Co-overexpression of the Constitutively Active Form of OsbZIP46 and ABA-Activated Protein Kinase SAPK6 Improves Drought and Temperature Stress Resistance in Rice.
- Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice.
- The function of OsSAPK6 in chilling tolerance depended on IPA1, and overexpression of OsCBF3 could rescue the chilling-sensitive phenotype of ipa1 loss-of-function mutant.
- Under chilling stress, OsSAPK6 could phosphorylate IPA1 and increase its stability.
Function-related keywords:
- vegetative , seed , drought , seed-germination , root , ABA , transcription-factor , temperature , resistance , stress , Kinase , protein-kinase , tolerance , chilling , chilling-stress
Literature:
- A rice dehydration-inducible SNF1-related protein kinase 2 phosphorylates an abscisic acid responsive element-binding factor and associates with ABA signaling . DOI: 10.1007/s11103-006-9079-x ; PMID: 16977424
- Overexpression of OsMYB48-1, a novel MYB-related transcription factor, enhances drought and salinity tolerance in rice . DOI: 10.1371/journal.pone.0092913 ; PMID: 24667379
- Differential activation of the rice sucrose nonfermenting1-related protein kinase2 family by hyperosmotic stress and abscisic acid . DOI: 10.1105/tpc.019943 ; PMID: 15084714
- Co-overexpression of the Constitutively Active Form of OsbZIP46 and ABA-Activated Protein Kinase SAPK6 Improves Drought and Temperature Stress Resistance in Rice . DOI: 10.3389/fpls.2017.01102 ; PMID: 28694815
- Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice . DOI: 10.1038/s41421-022-00413-2 ; PMID: 35882853
Related News:
Gene Resources:
- NCBI ID: AB125307
- UniProt accessions:
Sequences:
cDNA Sequence
- >LOC_Os02g34600.1
AGGAGGAGAGAGAAACCGCTACTAATAAATAGGAGTATAAAACAGAAGCCTCCTCGTTAAGCTTCGAATCCTCTTCCATTCCCTCCTTCCTCTCCTCTCCTCTCCAAGACCAAGAACCAGCAGGAGCAAGCAAGGAAGGCGCCGAATTCGATCGGAGCAGGCAGGACGGGTCCGGGATCCCGCGCCGCCATGGAGAAGTACGAGCTGCTCAAGGACATCGGGTCGGGCAACTTCGGTGTGGCGCGGCTGATGCGGAACAGGGAGACCAAGGAGCTCGTCGCCATGAAGTACATACCGCGTGGCCTCAAGATTGACGAGAATGTGGCGAGGGAGATCATAAACCACCGCTCGCTGCGGCACCCAAACATCATCCGGTTCAAGGAGGTCGTGCTCACGCCTACCCACCTCGCGATCGTCATGGAGTACGCCGCCGGCGGCGAGCTGTTCGACCGGATCTGCAGCGCCGGGAGATTCAGCGAGGACGAGTCGAGGTATTTCTTCCAGCAACTAATTTGCGGCGTCAGCTACTGCCACTTCATGCAAATTTGCCACCGGGATTTGAAGCTGGAGAACACGCTGCTGGATGGCAGCCCTGCGCCGCGCCTCAAGATCTGCGACTTTGGCTACTCCAAGTCATCACTGCTGCACTCAAAGCCGAAGTCGACGGTCGGGACTCCCGCGTACATCGCTCCGGAGGTGCTCTCTCGCCGGGAGTATGACGGCAAGATGGCAGATGTATGGTCTTGTGGGGTGACCCTTTATGTGATGCTCGTCGGTGCTTACCCTTTTGAGGACCCAGATGATCCCAAGAATTTCAGAAAAACAATCGGGAGAATCGTATCAATTCAGTACAAAATACCAGAGTACGTCCATATATCCCAAGATTGTAGACAACTCCTCTCTCGAATCTTTGTCGCGAATCCTGCAAAGAGAATAACAATAAGAGAGATCAGAAACCACCCTTGGTTTATGAAGAACTTGCCGCGGGAGCTTACAGAAGCGGCGCAAGCGAAGTACTACAAGAAGGACAACAGTGCCCGTACATTCTCGGATCAGACCGTCGACGAGATCATGAAGATTGTACAAGAGGCAAAGACACCACCTCCATCGTCGACTCCAGTGGCCGGTTTCGGTTGGACCGAGGAAGAAGAGCAGGAGGACGGTAAGAATCCCGACGACGACGAGGGAGACAGGGATGAGGAGGAGGGCGAGGAAGGCGATAGCGAGGACGAGTACACCAAGCAGGTGAAGCAAGCCCATGCCAGCTGTGACTTGCAGAAGAGCTGATAAGATGTTGAGTTCAAGCTTTGCTGTTGCCGGCAAGAGGGTGTTTTTGTGCATGTTTGTACTTTGTGGTAGTTGTGGGGGTGATGAGATGTCGCATCCTTTTCGTTATTAGTTTGTCTTTGTAGCTTTTAACTTTCTGTTGTTGCCTTGGGTCTTCCTTTTCTTTTATCCTGTTCTTTTTTTTTATCTTGTGATATGTTAACACCGTAAATTATTACTACCAAATAGGCTCTCCAGTGCCTTTGTAATGGTTGGATGGTTAAGATAGCAAGGTTGTATATAGCTTGTGGAATAAACCTTATTTCTTGACAAGTGTTTATTTAGGGAGACAATATTTTTTCTTTTCACCAACGAGATAAGCAACACAAATGTGTATATATGCATGAGATCTGTTTAACCTCATTCCACGTATTGAAATAATTTTTTTGCCTCTGGAC - >LOC_Os02g34600.2
AGGAGGAGAGAGAAACCGCTACTAATAAATAGGAGTATAAAACAGAAGCCTCCTCGTTAAGCTTCGAATCCTCTTCCATTCCCTCCTTCCTCTCCTCTCCTCTCCAAGACCAAGAACCAGCAGGAGCAAGCAAGGAAGGCGCCGAATTCGATCGGAGCAGGCAGGACGGGTCCGGGATCCCGCGCCGCCATGGAGAAGTACGAGCTGCTCAAGGACATCGGGTCGGGCAACTTCGGTGTGGCGCGGCTGATGCGGAACAGGGAGACCAAGGAGCTCGTCGCCATGAAGTACATACCGCGTGGCCTCAAGATTGACGAGAATGTGGCGAGGGAGATCATAAACCACCGCTCGCTGCGGCACCCAAACATCATCCGGTTCAAGGAGGTCGTGCTCACGCCTACCCACCTCGCGATCGTCATGGAGTACGCCGCCGGCGGCGAGCTGTTCGACCGGATCTGCAGCGCCGGGAGATTCAGCGAGGACGAGTCGAGGTATTTCTTCCAGCAACTAATTTGCGGCGTCAGCTACTGCCACTTCATGCAAATTTGCCACCGGGATTTGAAGCTGGAGAACACGCTGCTGGATGGCAGCCCTGCGCCGCGCCTCAAGATCTGCGACTTTGGCTACTCCAAGTCATCACTGCTGCACTCAAAGCCGAAGTCGACGGTCGGGACTCCCGCGTACATCGCTCCGGAGGTGCTCTCTCGCCGGGAGTATGACGGCAAGATGGCAGATGTATGGTCTTGTGGGGTGACCCTTTATGTGATGCTCGTCGGTGCTTACCCTTTTGAGGACCCAGATGATCCCAAGAATTTCAGAAAAACAATCGGGAGAATCGTATCAATTCAGTACAAAATACCAGAGTACGTCCATATATCCCAAGATTGTAGACAACTCCTCTCTCGAATCTTTGTCGCGAATCCTGCAAAGAGAATAACAATAAGAGAGATCAGAAACCACCCTTGGTTTATGAAGAACTTGCCGCGGGAGCTTACAGAAGCGGCGCAAGCGAAGTACTACAAGAAGGACAACAGTGCCCGTACATTCTCGGATCAGACCGTCGACGAGATCATGAAGATTGTACAAGAGGCAAAGACACCACCTCCATCGTCGACTCCAGTGGCCGGTTTCGGTTGGACCGAGGAAGAAGAGCAGGAGGACGGTAAGAATCCCGACGACGACGAGGGAGACAGGGATGAGGAGGAGGGCGAGGAAGGCGATAGCGAGGACGAGTACACCAAGCAGGTGAAGCAAGCCCATGCCAGCTGTGACTTGCAGAAGAGCTGATAAGATGTTGAGTTCAAGCTTTGCTGTTGCCGGCAAGAGGGTGTTTTTGTGCATGTTTGTACTTTGTGGTAGTTGTGGGGGTGATGAGATGTCGCATCCTTTTCGTTATTAGTTTGTCTTTGTAGCTTTTAACTTTCTGTTGTTGCCTTGGGTCTTCCTTTTCTTTTATCCTGTTCTTTTTTTTTATCTTGTGATATGTTAACACCGTAAATTATTACTACCAAATAGGCTCTCCAGTGCCTTTGTAATGGTTGGATGGTTAAGATAGCAAGGTTGTATATAGCTTGTGGAATAAACCTTATTTCTTGACAAGTGTTTATTTAGGGAGACAATATTTTTTCTTTTCACCAACGAGATAAGCAACACAAATGTGTATATATGCATGAGATCTGTTTAACCTCATTCCACGTATTGAAATAATTTTTTTGCCTCTGGAC
CDS Sequence
- >LOC_Os02g34600.1
ATGGAGAAGTACGAGCTGCTCAAGGACATCGGGTCGGGCAACTTCGGTGTGGCGCGGCTGATGCGGAACAGGGAGACCAAGGAGCTCGTCGCCATGAAGTACATACCGCGTGGCCTCAAGATTGACGAGAATGTGGCGAGGGAGATCATAAACCACCGCTCGCTGCGGCACCCAAACATCATCCGGTTCAAGGAGGTCGTGCTCACGCCTACCCACCTCGCGATCGTCATGGAGTACGCCGCCGGCGGCGAGCTGTTCGACCGGATCTGCAGCGCCGGGAGATTCAGCGAGGACGAGTCGAGGTATTTCTTCCAGCAACTAATTTGCGGCGTCAGCTACTGCCACTTCATGCAAATTTGCCACCGGGATTTGAAGCTGGAGAACACGCTGCTGGATGGCAGCCCTGCGCCGCGCCTCAAGATCTGCGACTTTGGCTACTCCAAGTCATCACTGCTGCACTCAAAGCCGAAGTCGACGGTCGGGACTCCCGCGTACATCGCTCCGGAGGTGCTCTCTCGCCGGGAGTATGACGGCAAGATGGCAGATGTATGGTCTTGTGGGGTGACCCTTTATGTGATGCTCGTCGGTGCTTACCCTTTTGAGGACCCAGATGATCCCAAGAATTTCAGAAAAACAATCGGGAGAATCGTATCAATTCAGTACAAAATACCAGAGTACGTCCATATATCCCAAGATTGTAGACAACTCCTCTCTCGAATCTTTGTCGCGAATCCTGCAAAGAGAATAACAATAAGAGAGATCAGAAACCACCCTTGGTTTATGAAGAACTTGCCGCGGGAGCTTACAGAAGCGGCGCAAGCGAAGTACTACAAGAAGGACAACAGTGCCCGTACATTCTCGGATCAGACCGTCGACGAGATCATGAAGATTGTACAAGAGGCAAAGACACCACCTCCATCGTCGACTCCAGTGGCCGGTTTCGGTTGGACCGAGGAAGAAGAGCAGGAGGACGGTAAGAATCCCGACGACGACGAGGGAGACAGGGATGAGGAGGAGGGCGAGGAAGGCGATAGCGAGGACGAGTACACCAAGCAGGTGAAGCAAGCCCATGCCAGCTGTGACTTGCAGAAGAGCTGA - >LOC_Os02g34600.2
ATGGAGAAGTACGAGCTGCTCAAGGACATCGGGTCGGGCAACTTCGGTGTGGCGCGGCTGATGCGGAACAGGGAGACCAAGGAGCTCGTCGCCATGAAGTACATACCGCGTGGCCTCAAGATTGACGAGAATGTGGCGAGGGAGATCATAAACCACCGCTCGCTGCGGCACCCAAACATCATCCGGTTCAAGGAGGTCGTGCTCACGCCTACCCACCTCGCGATCGTCATGGAGTACGCCGCCGGCGGCGAGCTGTTCGACCGGATCTGCAGCGCCGGGAGATTCAGCGAGGACGAGTCGAGGTATTTCTTCCAGCAACTAATTTGCGGCGTCAGCTACTGCCACTTCATGCAAATTTGCCACCGGGATTTGAAGCTGGAGAACACGCTGCTGGATGGCAGCCCTGCGCCGCGCCTCAAGATCTGCGACTTTGGCTACTCCAAGTCATCACTGCTGCACTCAAAGCCGAAGTCGACGGTCGGGACTCCCGCGTACATCGCTCCGGAGGTGCTCTCTCGCCGGGAGTATGACGGCAAGATGGCAGATGTATGGTCTTGTGGGGTGACCCTTTATGTGATGCTCGTCGGTGCTTACCCTTTTGAGGACCCAGATGATCCCAAGAATTTCAGAAAAACAATCGGGAGAATCGTATCAATTCAGTACAAAATACCAGAGTACGTCCATATATCCCAAGATTGTAGACAACTCCTCTCTCGAATCTTTGTCGCGAATCCTGCAAAGAGAATAACAATAAGAGAGATCAGAAACCACCCTTGGTTTATGAAGAACTTGCCGCGGGAGCTTACAGAAGCGGCGCAAGCGAAGTACTACAAGAAGGACAACAGTGCCCGTACATTCTCGGATCAGACCGTCGACGAGATCATGAAGATTGTACAAGAGGCAAAGACACCACCTCCATCGTCGACTCCAGTGGCCGGTTTCGGTTGGACCGAGGAAGAAGAGCAGGAGGACGGTAAGAATCCCGACGACGACGAGGGAGACAGGGATGAGGAGGAGGGCGAGGAAGGCGATAGCGAGGACGAGTACACCAAGCAGGTGAAGCAAGCCCATGCCAGCTGTGACTTGCAGAAGAGCTGA
Protein Sequence
- >LOC_Os02g34600.1
MEKYELLKDIGSGNFGVARLMRNRETKELVAMKYIPRGLKIDENVAREIINHRSLRHPNIIRFKEVVLTPTHLAIVMEYAAGGELFDRICSAGRFSEDESRYFFQQLICGVSYCHFMQICHRDLKLENTLLDGSPAPRLKICDFGYSKSSLLHSKPKSTVGTPAYIAPEVLSRREYDGKMADVWSCGVTLYVMLVGAYPFEDPDDPKNFRKTIGRIVSIQYKIPEYVHISQDCRQLLSRIFVANPAKRITIREIRNHPWFMKNLPRELTEAAQAKYYKKDNSARTFSDQTVDEIMKIVQEAKTPPPSSTPVAGFGWTEEEEQEDGKNPDDDEGDRDEEEGEEGDSEDEYTKQVKQAHASCDLQKS* - >LOC_Os02g34600.2
MEKYELLKDIGSGNFGVARLMRNRETKELVAMKYIPRGLKIDENVAREIINHRSLRHPNIIRFKEVVLTPTHLAIVMEYAAGGELFDRICSAGRFSEDESRYFFQQLICGVSYCHFMQICHRDLKLENTLLDGSPAPRLKICDFGYSKSSLLHSKPKSTVGTPAYIAPEVLSRREYDGKMADVWSCGVTLYVMLVGAYPFEDPDDPKNFRKTIGRIVSIQYKIPEYVHISQDCRQLLSRIFVANPAKRITIREIRNHPWFMKNLPRELTEAAQAKYYKKDNSARTFSDQTVDEIMKIVQEAKTPPPSSTPVAGFGWTEEEEQEDGKNPDDDEGDRDEEEGEEGDSEDEYTKQVKQAHASCDLQKS*