Information report for OsIPK2;IPK2
Gene Details
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Functional Descriptions
- CONCLUSIONS: The analysis we conducted in plant mitochondria provided the negative, though we argue relevant, result that IP6K does not actually occur in vegetable mtDNA.
- On the other hand, the up-regulation of a MIPS, an IMP, an IPK2, and an ITP5/6K in anthers suggested that a PLC-mediated pathway was active in addition to a lipid-independent pathway in the anthers.
- Furthermore, expressing additional OsIPK2 or its N-terminal amino acid sequence enhanced the accumulation of OsIAA11 protein in transgenic plants, which in turn caused defects in lateral root formation and auxin response.
- Conclusions: The ES2 gene, encoding an inositol polyphosphate kinase localized in the nucleus and plasma membrane of cells, is essential for leaf senescence in rice.
- Further study of ES2 will facilitate the dissection of the genetic mechanisms underlying early leaf senescence and plant growth.
- Leaves of ES2 showed early senescence at the seedling stage and became severe at the tillering stage.
- Moreover, genes which related to senescence, ROS and chlorophyll degradation were up-regulated, while those associated with photosynthesis and chlorophyll synthesis were down-regulated in ES2 mutant compared to WYG7.
- DNA sequencing of ES2 in the mutant revealed a missense mutation, ES2 was localized to nucleus and plasma membrane of cells, and expressed in various tissues of rice.
- The ES2 gene, which encodes an inositol polyphosphate kinase (OsIPK2), was fine mapped to a 116.
- The contents of reactive oxygen species (ROS) significantly increased, while chlorophyll content, photosynthetic rate, catalase (CAT) activity significantly decreased in the ES2 mutant.
- Complementation test and overexpression experiment confirmed that ES2 completely restored the normal phenotype, with chlorophyll contents and photosynthetic rate increased comparable with the wild type.
Functional Keywords
- mitochondria , anther , root , auxin , lateral-root , auxin-response , leaf , leaf-senescence , early-leaf-senescence , senescence , tillering , growth , seedling , photosynthesis , nucleus , plant-growth , Kinase , plasma-membrane , chlorophyll-content , reactive-oxygen-species , kinase
Literature and News
- IP6K gene identification in plant genomes by tag searching . DOI: 10.1186/1753-6561-5-S2-S1 ; PMID: 21554757
- Expression pattern of inositol phosphate-related enzymes in rice (Oryza sativa L.): implications for the phytic acid biosynthetic pathway . DOI: 10.1016/j.gene.2007.09.006 ; PMID: 17961936
- Rice Inositol Polyphosphate Kinase (OsIPK2) Directly Interacts with OsIAA11 to Regulate Lateral Root Formation . DOI: 10.1093/pcp/pcx125 ; PMID: 29016933
- Rice EARLY SENESCENCE 2, encoding an inositol polyphosphate kinase, is involved in leaf senescence . DOI: 10.1186/s12870-020-02610-1 ; PMID: 32847519
Gene Resources
- UniProt: Q6H545
- EMBL: AP004772, AP005749, AP008208
- AlphaFoldDB: Q6H545
- EnsemblPlants: Os02t0523800-01
- Gramene: Os02t0523800-01
- KEGG: dosa:Os02g0523800
- Orthologous matrix: DCAFAAT
- InterPro: IPR005522, IPR038286
- PANTHER: PTHR12400, PTHR12400:SF51
- SUPFAM: SSF56104
- Gene3D: 3.30.470.160
- OrthoDB: Q6H545
- SWISS-MODEL: Q6H545
- eggNOG: KOG1620
Sequences
cDNA Sequence
- >LOC_Os02g32370.1
GTGGCCGGCGAGCTCCCGTCCATTCCCTCGTCTCTCTGGATAGGGGATCTGGAAAATTTGCTGCTCTCTTGATCGAGCGGTGAAACTTTCTCACCATTCATCCATCCGGCGAAACTTCCACTGATGAATCGGACTTCCCAAACCTTTCCACGCTCTCGCTCCAAATGACCAAAAGCCAGAGGAGGAGGGTTTCGCAGCAACAACGTGGGCGCCCACGGCCCGCGCCCGGTCAGCGAGGAAACAGATCGAAAAAAAACAAAAGCCGCTGACGTCACGATCTCGAGGCTCGCGATTCCCAACAATCTCTTTAAAAAGCTTCCTCTCGCCGAATCCCCCTCCACCTCCACCACCCGCCGCCGCCGCCGCCGCCGCCGCCATGGCCTCCGACCTGCGCCCGCCGGAGCACCAGGTGGCGGGGCACCGCGCGTCCGCCGACAAGCTGGGCCCGCTCGTCGACGGCGAGGGGCTCTTCTACAAGCCCCTCCAGGCCGGGGAGCGCGGGGAGCACGAGGCCGCCTTCTACGCCGCGTTCACCGCGCACCCGGCCGTCCCGCCCCGGGTCCGGGGCGCCTTCTTCCCGCGCTTCCACGGCACCCGCTTCCTCCCGGCCCCAGCCAGCCCCGGCGGCGCGCCCTACCCGCACATCGTCCTCGACGACCTCCTCGCGGGCCTCCCGTCCCCCTGCGTCGCCGACGTCAAGATCGGCGCCTGCACGTGGCCGCCGCGATCCCCGGACCCCTACGTCGCCAAGTGCCTCGCCAAGGACCGCGAGACCACCAGCGCGCTCCTCGGCTTCCGCGTCTCCGGCGTCCGGGTGGTCGATGCCCGGGGCGGCGCCGTGTGGCGCCCGGACCGGTCGGAGCTGAAGGGGATCGACGCCGCCGGGGTCCGCCGCGTGCTCCGCCGCTACGTGTCCACGGGCGGCGGCGACGGCCTGGACTGCGCGCTCGCCGCCGCGGTGTACGGAGGGGAGGGCGGCGTCCTGGCTCAGCTGCGGGAGCTCAAGGCGTGGTTCGAGGAGCAAACCCTGTACCACTTCTACTCGGCGTCGATTCTGTTCGGCTACGACGCCAATGCGGCGGCGGCGGCTGCTCCCGGAGGTGGAAGCGGCGGTGTAAGGGTGAAGCTGGTGGACTTCGCGCATGTCGACGATGGGGACGGGGTGATTGACCACAACTTCTTGGGCGGGCTCTGCTCGCTCATCAAGTTCATCGGCGACATTGTCGCCGAGGTTACCGAGAAGGCGTCTTCAGATCATTCTTGAAAATTTTGTCAGCTTGGAGATCAGATGATTAAACGGTCCAACATTGTGTTCTTGCAAATGTCACACATAATAAAGTGCCATCAAATGGAGAAAGAGTCAATTGTTTGAAAGAGGAAACTTCACCGAGCTGCCGGTAACGTAAATGCGAAGCGACTCACCGGCAGCTCTCCCGGCAAACTTACGGCAACTGATACCTTTTTTTGCATACCCCCCTTTAAAACCATGGGATTCGGGGTGCATTGGATCTTACTCTTTAGTTGTATTAAAGAAATGGTGAACAAAAGATGGGCGTTGTCATTCCACCGAGAAAGAGTTCTTTCGCTATATTTCATACTTGCAGTCTCATAGGGAATTTTGCAAAGAATTGTCCGAGCATCATTGCAGGCCAAACACCTGCCTTTTCTGAAAACATATTTATACGAACAGACATCTAACGAATGTTTTTCTTGTATGAAAGTCGACCATAAGCACCAAACTCTGATATTCGAAACCTAGTGGTGTGGTATTAACCATTTTGTGTAA
CDS Sequence
- >LOC_Os02g32370.1
ATGGCCTCCGACCTGCGCCCGCCGGAGCACCAGGTGGCGGGGCACCGCGCGTCCGCCGACAAGCTGGGCCCGCTCGTCGACGGCGAGGGGCTCTTCTACAAGCCCCTCCAGGCCGGGGAGCGCGGGGAGCACGAGGCCGCCTTCTACGCCGCGTTCACCGCGCACCCGGCCGTCCCGCCCCGGGTCCGGGGCGCCTTCTTCCCGCGCTTCCACGGCACCCGCTTCCTCCCGGCCCCAGCCAGCCCCGGCGGCGCGCCCTACCCGCACATCGTCCTCGACGACCTCCTCGCGGGCCTCCCGTCCCCCTGCGTCGCCGACGTCAAGATCGGCGCCTGCACGTGGCCGCCGCGATCCCCGGACCCCTACGTCGCCAAGTGCCTCGCCAAGGACCGCGAGACCACCAGCGCGCTCCTCGGCTTCCGCGTCTCCGGCGTCCGGGTGGTCGATGCCCGGGGCGGCGCCGTGTGGCGCCCGGACCGGTCGGAGCTGAAGGGGATCGACGCCGCCGGGGTCCGCCGCGTGCTCCGCCGCTACGTGTCCACGGGCGGCGGCGACGGCCTGGACTGCGCGCTCGCCGCCGCGGTGTACGGAGGGGAGGGCGGCGTCCTGGCTCAGCTGCGGGAGCTCAAGGCGTGGTTCGAGGAGCAAACCCTGTACCACTTCTACTCGGCGTCGATTCTGTTCGGCTACGACGCCAATGCGGCGGCGGCGGCTGCTCCCGGAGGTGGAAGCGGCGGTGTAAGGGTGAAGCTGGTGGACTTCGCGCATGTCGACGATGGGGACGGGGTGATTGACCACAACTTCTTGGGCGGGCTCTGCTCGCTCATCAAGTTCATCGGCGACATTGTCGCCGAGGTTACCGAGAAGGCGTCTTCAGATCATTCTTGA
Protein Sequence
- >LOC_Os02g32370.1
MASDLRPPEHQVAGHRASADKLGPLVDGEGLFYKPLQAGERGEHEAAFYAAFTAHPAVPPRVRGAFFPRFHGTRFLPAPASPGGAPYPHIVLDDLLAGLPSPCVADVKIGACTWPPRSPDPYVAKCLAKDRETTSALLGFRVSGVRVVDARGGAVWRPDRSELKGIDAAGVRRVLRRYVSTGGGDGLDCALAAAVYGGEGGVLAQLRELKAWFEEQTLYHFYSASILFGYDANAAAAAAPGGGSGGVRVKLVDFAHVDDGDGVIDHNFLGGLCSLIKFIGDIVAEVTEKASSDHS*