Gene Details:

Functional Descriptions:

  • CONCLUSIONS: The analysis we conducted in plant mitochondria provided the negative, though we argue relevant, result that IP6K does not actually occur in vegetable mtDNA.
  • On the other hand, the up-regulation of a MIPS, an IMP, an IPK2, and an ITP5/6K in anthers suggested that a PLC-mediated pathway was active in addition to a lipid-independent pathway in the anthers.
  • Furthermore, expressing additional OsIPK2 or its N-terminal amino acid sequence enhanced the accumulation of OsIAA11 protein in transgenic plants, which in turn caused defects in lateral root formation and auxin response.
  • Conclusions: The ES2 gene, encoding an inositol polyphosphate kinase localized in the nucleus and plasma membrane of cells, is essential for leaf senescence in rice.
  • Further study of ES2 will facilitate the dissection of the genetic mechanisms underlying early leaf senescence and plant growth.
  • Leaves of ES2 showed early senescence at the seedling stage and became severe at the tillering stage.
  • Moreover, genes which related to senescence, ROS and chlorophyll degradation were up-regulated, while those associated with photosynthesis and chlorophyll synthesis were down-regulated in ES2 mutant compared to WYG7.
  • DNA sequencing of ES2 in the mutant revealed a missense mutation, ES2 was localized to nucleus and plasma membrane of cells, and expressed in various tissues of rice.
  • The ES2 gene, which encodes an inositol polyphosphate kinase (OsIPK2), was fine mapped to a 116.
  • The contents of reactive oxygen species (ROS) significantly increased, while chlorophyll content, photosynthetic rate, catalase (CAT) activity significantly decreased in the ES2 mutant.
  • Complementation test and overexpression experiment confirmed that ES2 completely restored the normal phenotype, with chlorophyll contents and photosynthetic rate increased comparable with the wild type.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os02g32370.1
    GTGGCCGGCGAGCTCCCGTCCATTCCCTCGTCTCTCTGGATAGGGGATCTGGAAAATTTGCTGCTCTCTTGATCGAGCGGTGAAACTTTCTCACCATTCATCCATCCGGCGAAACTTCCACTGATGAATCGGACTTCCCAAACCTTTCCACGCTCTCGCTCCAAATGACCAAAAGCCAGAGGAGGAGGGTTTCGCAGCAACAACGTGGGCGCCCACGGCCCGCGCCCGGTCAGCGAGGAAACAGATCGAAAAAAAACAAAAGCCGCTGACGTCACGATCTCGAGGCTCGCGATTCCCAACAATCTCTTTAAAAAGCTTCCTCTCGCCGAATCCCCCTCCACCTCCACCACCCGCCGCCGCCGCCGCCGCCGCCGCCATGGCCTCCGACCTGCGCCCGCCGGAGCACCAGGTGGCGGGGCACCGCGCGTCCGCCGACAAGCTGGGCCCGCTCGTCGACGGCGAGGGGCTCTTCTACAAGCCCCTCCAGGCCGGGGAGCGCGGGGAGCACGAGGCCGCCTTCTACGCCGCGTTCACCGCGCACCCGGCCGTCCCGCCCCGGGTCCGGGGCGCCTTCTTCCCGCGCTTCCACGGCACCCGCTTCCTCCCGGCCCCAGCCAGCCCCGGCGGCGCGCCCTACCCGCACATCGTCCTCGACGACCTCCTCGCGGGCCTCCCGTCCCCCTGCGTCGCCGACGTCAAGATCGGCGCCTGCACGTGGCCGCCGCGATCCCCGGACCCCTACGTCGCCAAGTGCCTCGCCAAGGACCGCGAGACCACCAGCGCGCTCCTCGGCTTCCGCGTCTCCGGCGTCCGGGTGGTCGATGCCCGGGGCGGCGCCGTGTGGCGCCCGGACCGGTCGGAGCTGAAGGGGATCGACGCCGCCGGGGTCCGCCGCGTGCTCCGCCGCTACGTGTCCACGGGCGGCGGCGACGGCCTGGACTGCGCGCTCGCCGCCGCGGTGTACGGAGGGGAGGGCGGCGTCCTGGCTCAGCTGCGGGAGCTCAAGGCGTGGTTCGAGGAGCAAACCCTGTACCACTTCTACTCGGCGTCGATTCTGTTCGGCTACGACGCCAATGCGGCGGCGGCGGCTGCTCCCGGAGGTGGAAGCGGCGGTGTAAGGGTGAAGCTGGTGGACTTCGCGCATGTCGACGATGGGGACGGGGTGATTGACCACAACTTCTTGGGCGGGCTCTGCTCGCTCATCAAGTTCATCGGCGACATTGTCGCCGAGGTTACCGAGAAGGCGTCTTCAGATCATTCTTGAAAATTTTGTCAGCTTGGAGATCAGATGATTAAACGGTCCAACATTGTGTTCTTGCAAATGTCACACATAATAAAGTGCCATCAAATGGAGAAAGAGTCAATTGTTTGAAAGAGGAAACTTCACCGAGCTGCCGGTAACGTAAATGCGAAGCGACTCACCGGCAGCTCTCCCGGCAAACTTACGGCAACTGATACCTTTTTTTGCATACCCCCCTTTAAAACCATGGGATTCGGGGTGCATTGGATCTTACTCTTTAGTTGTATTAAAGAAATGGTGAACAAAAGATGGGCGTTGTCATTCCACCGAGAAAGAGTTCTTTCGCTATATTTCATACTTGCAGTCTCATAGGGAATTTTGCAAAGAATTGTCCGAGCATCATTGCAGGCCAAACACCTGCCTTTTCTGAAAACATATTTATACGAACAGACATCTAACGAATGTTTTTCTTGTATGAAAGTCGACCATAAGCACCAAACTCTGATATTCGAAACCTAGTGGTGTGGTATTAACCATTTTGTGTAA
CDS Sequence
  • >LOC_Os02g32370.1
    ATGGCCTCCGACCTGCGCCCGCCGGAGCACCAGGTGGCGGGGCACCGCGCGTCCGCCGACAAGCTGGGCCCGCTCGTCGACGGCGAGGGGCTCTTCTACAAGCCCCTCCAGGCCGGGGAGCGCGGGGAGCACGAGGCCGCCTTCTACGCCGCGTTCACCGCGCACCCGGCCGTCCCGCCCCGGGTCCGGGGCGCCTTCTTCCCGCGCTTCCACGGCACCCGCTTCCTCCCGGCCCCAGCCAGCCCCGGCGGCGCGCCCTACCCGCACATCGTCCTCGACGACCTCCTCGCGGGCCTCCCGTCCCCCTGCGTCGCCGACGTCAAGATCGGCGCCTGCACGTGGCCGCCGCGATCCCCGGACCCCTACGTCGCCAAGTGCCTCGCCAAGGACCGCGAGACCACCAGCGCGCTCCTCGGCTTCCGCGTCTCCGGCGTCCGGGTGGTCGATGCCCGGGGCGGCGCCGTGTGGCGCCCGGACCGGTCGGAGCTGAAGGGGATCGACGCCGCCGGGGTCCGCCGCGTGCTCCGCCGCTACGTGTCCACGGGCGGCGGCGACGGCCTGGACTGCGCGCTCGCCGCCGCGGTGTACGGAGGGGAGGGCGGCGTCCTGGCTCAGCTGCGGGAGCTCAAGGCGTGGTTCGAGGAGCAAACCCTGTACCACTTCTACTCGGCGTCGATTCTGTTCGGCTACGACGCCAATGCGGCGGCGGCGGCTGCTCCCGGAGGTGGAAGCGGCGGTGTAAGGGTGAAGCTGGTGGACTTCGCGCATGTCGACGATGGGGACGGGGTGATTGACCACAACTTCTTGGGCGGGCTCTGCTCGCTCATCAAGTTCATCGGCGACATTGTCGCCGAGGTTACCGAGAAGGCGTCTTCAGATCATTCTTGA
Protein Sequence
  • >LOC_Os02g32370.1
    MASDLRPPEHQVAGHRASADKLGPLVDGEGLFYKPLQAGERGEHEAAFYAAFTAHPAVPPRVRGAFFPRFHGTRFLPAPASPGGAPYPHIVLDDLLAGLPSPCVADVKIGACTWPPRSPDPYVAKCLAKDRETTSALLGFRVSGVRVVDARGGAVWRPDRSELKGIDAAGVRRVLRRYVSTGGGDGLDCALAAAVYGGEGGVLAQLRELKAWFEEQTLYHFYSASILFGYDANAAAAAAPGGGSGGVRVKLVDFAHVDDGDGVIDHNFLGGLCSLIKFIGDIVAEVTEKASSDHS*