Gene Details:

Functional Descriptions:

  • Cytological and transcriptome analyses reveal OsPUB73 defect affects the gene expression associated with tapetum or pollen exine abnormality in rice.
  • In the present study, we knocked out OsPUB73 by using the CRISPR/Cas9 system and studied the cytological and transcriptome of the gene-defect associated with pollen development and sterility in the rice variety (Taichung 65).
  • The OsPUB73 displayed low pollen fertility (19.
  • Cytological observation showed tapetum vacuolated at the meiosis stage and pollen exine was abnormal at the bi-cellular pollen stage of OsPUB73.
  • These results suggested OsPUB73 may play an important role in tapetal or pollen exine development and resulted in pollen partial sterility.
  • The expression pattern of OsPUB73 showed that it was highly expressed in the anther during meiosis stage.
  • RNA-seq analysis detected 2240 down and 571 up-regulated genes in anther of OsPUB73 compared with WT during meiosis stage.
  • Our results revealed that OsPUB73 plays an important role in rice male reproductive development, which provides valuable information about the molecular mechanisms of the U-box in rice male reproductive development.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os02g28870.1
    ATCCAAACACCTCTTCACATTTTTCCCTCCAAGAAACCGACCCGCTCCACTCCACCCCGCAACGCAAGAAACCAAGAACTCCTCGACCGCCGGCTCGAGGCCACGGCCACCCACCCACCGCCGCCGGGAAGCAGCGATTCTTCCTCGGGAAGGAGGACACCGCCGGCCATGGATCCGGAGGCGGAGGAAGCGCAGCTTCGCCTCGAGATGGAGTTGGCAAAGAAGGCCAAGGCGGACATGTCTGGGTTGCAACGCTCCAGCAGCCTCGGCTTGGACCACGCCGGCTTGTATCCGCTGCCATTGCCGCCAGGGTGGCGGTCCGCCCCAACCTCGCCCCTGAGGACACCATCGAGCCCGCCCCCGCTCCAGTTCCCGCCGGCGTGGGCGGCTGATGTTGCCGGCACGTCGGGCTCGGCGGCGCCCGAGGACGATGGCCCAGCAAGAAATGCCGGCGCTGATGAAGCCACGGCTGGGTCTGCGCCCAAGAACGAGGACCCAGCAAGAGCTGCGGGCGCCGATGATGGCCCCACGAGGTCTGACTACGCCGCGATGATGCGGATGGCATTGGCCAAGTTCCAAGACGACGATGCTGCTGCCGACGACGAGGAGGCGGCGTCCGCGGTGATGGAGCAGGCGATGACCGGCCTCATGGACCTCACCTACCGCAAAGCGAAGCCTCCAGAGCTGCCTTACGAGTTCGCCACAAGATGGCCTATTCCTATTGCTCATGATGGTACGCTTCAAGCGGAAGTAATGCGTGATCCTGTTATCTTGCCTTCTGGATATTCTGTTGATCAAACATACCAAAACAACCAAAAACGGCAGAATCCATGGACAAATACCAGCACATTTACTGATCACTCCTTGCCCTACTCTCTCTCTGTTCCAAACCACCTTCTCCGTGACATGATTTCTGCATGGTGCCTCGATCACTCGGACCTTTCCCCATCTACCACTTCTGATACACCTTCCACTCCCTTGGAACCTTCAGAGGAGGAACAAATTCAACGCATCCTAAAACTATTCTCAGGGAATTCTGCATCGCAACGAGAGGCTTTGAAACTGATCCAGCTACTCACTAAAACCACCAAAGGGGTGCAACCTTGCCTTGCCAAGTATGCGGACATTATCCCAGTGCTGATAAATCTTAGGAGGAAATATAAATCGAGTTGGACACAAGATCTTGAGGAAGAGAGGCTCACAATAATCCTCAATCTGACCATGCACAGGCAAAACAGGGAGATCCTAGCTGGACAGAATGAATTAGCAGGTGCTATAAAAAAAATTGTTAAGAAGGCTGGCAATCGTGGTAAGCGAACATCATCCTTGGCGAAGGTAGCTTCTATAGTTGCTGTCCTGTCAGAGTTTGACATGTTCAGGAAAAGAATGCTGGATGCTGGTGGAATGAAAATGCTTCGAGGTATGCTCAAGATAAAGGATACTGAAGTGATAACGGAGGCTGCCACTGCGATCCTTGCACTCTACGCAGATGGTGAAGGCGAGCAACCAGCCAGATTTCATGAGGTGCCTCAGATGCTGCTGGAATGCCACATGTTCACTGATGGGATCCTGCTTCTTCTTGACCGTCTGCCAAAATCCCCACGTGTTTTCAGAAAAATCTGTGATCAGGCTTTGCAATTGGTGAATATCGTCATGGCTGAAGATGCAAGTGGGCCGGTGACACGTAAGGGCATCCTCTCAGCAATTTCCTTGATCTACGAGATTGTTGAAAGGGATGTGGGTAAGATGAATGCGGTGAAGAACATGGAGGATTTCATCGAGCGGTTGCGTCAGCTATCATCAGATAGATTGCCGATGCAGAAAATGCTCCAGGTGGAGAGAATAATCAGGACTCTGTCAGATGCGTTTCCAGCTCCTACGGTGCGAGGGCGATGCCAAGAACCCAGTGGAAGCCGTCTTCTAGCTTGAATCTTATACTGTTCTTCTGTACAGATCTCAAAAGGCTCGAGATTTTACTTCTGCTCGCTTAACTTTCTGGAGAAGTTTGAATTTGTTGACTGTGCAGCAATGTGATGGATCACACGAACAATTCAAAGCATAGACAGAATAACTTTGTTTCACTTCCTGGTGAGAGATTGTAACAAATGATTGGTGTTAGTAACTGACCGAATTGA
CDS Sequence
  • >LOC_Os02g28870.1
    ATGGATCCGGAGGCGGAGGAAGCGCAGCTTCGCCTCGAGATGGAGTTGGCAAAGAAGGCCAAGGCGGACATGTCTGGGTTGCAACGCTCCAGCAGCCTCGGCTTGGACCACGCCGGCTTGTATCCGCTGCCATTGCCGCCAGGGTGGCGGTCCGCCCCAACCTCGCCCCTGAGGACACCATCGAGCCCGCCCCCGCTCCAGTTCCCGCCGGCGTGGGCGGCTGATGTTGCCGGCACGTCGGGCTCGGCGGCGCCCGAGGACGATGGCCCAGCAAGAAATGCCGGCGCTGATGAAGCCACGGCTGGGTCTGCGCCCAAGAACGAGGACCCAGCAAGAGCTGCGGGCGCCGATGATGGCCCCACGAGGTCTGACTACGCCGCGATGATGCGGATGGCATTGGCCAAGTTCCAAGACGACGATGCTGCTGCCGACGACGAGGAGGCGGCGTCCGCGGTGATGGAGCAGGCGATGACCGGCCTCATGGACCTCACCTACCGCAAAGCGAAGCCTCCAGAGCTGCCTTACGAGTTCGCCACAAGATGGCCTATTCCTATTGCTCATGATGGTACGCTTCAAGCGGAAGTAATGCGTGATCCTGTTATCTTGCCTTCTGGATATTCTGTTGATCAAACATACCAAAACAACCAAAAACGGCAGAATCCATGGACAAATACCAGCACATTTACTGATCACTCCTTGCCCTACTCTCTCTCTGTTCCAAACCACCTTCTCCGTGACATGATTTCTGCATGGTGCCTCGATCACTCGGACCTTTCCCCATCTACCACTTCTGATACACCTTCCACTCCCTTGGAACCTTCAGAGGAGGAACAAATTCAACGCATCCTAAAACTATTCTCAGGGAATTCTGCATCGCAACGAGAGGCTTTGAAACTGATCCAGCTACTCACTAAAACCACCAAAGGGGTGCAACCTTGCCTTGCCAAGTATGCGGACATTATCCCAGTGCTGATAAATCTTAGGAGGAAATATAAATCGAGTTGGACACAAGATCTTGAGGAAGAGAGGCTCACAATAATCCTCAATCTGACCATGCACAGGCAAAACAGGGAGATCCTAGCTGGACAGAATGAATTAGCAGGTGCTATAAAAAAAATTGTTAAGAAGGCTGGCAATCGTGGTAAGCGAACATCATCCTTGGCGAAGGTAGCTTCTATAGTTGCTGTCCTGTCAGAGTTTGACATGTTCAGGAAAAGAATGCTGGATGCTGGTGGAATGAAAATGCTTCGAGGTATGCTCAAGATAAAGGATACTGAAGTGATAACGGAGGCTGCCACTGCGATCCTTGCACTCTACGCAGATGGTGAAGGCGAGCAACCAGCCAGATTTCATGAGGTGCCTCAGATGCTGCTGGAATGCCACATGTTCACTGATGGGATCCTGCTTCTTCTTGACCGTCTGCCAAAATCCCCACGTGTTTTCAGAAAAATCTGTGATCAGGCTTTGCAATTGGTGAATATCGTCATGGCTGAAGATGCAAGTGGGCCGGTGACACGTAAGGGCATCCTCTCAGCAATTTCCTTGATCTACGAGATTGTTGAAAGGGATGTGGGTAAGATGAATGCGGTGAAGAACATGGAGGATTTCATCGAGCGGTTGCGTCAGCTATCATCAGATAGATTGCCGATGCAGAAAATGCTCCAGGTGGAGAGAATAATCAGGACTCTGTCAGATGCGTTTCCAGCTCCTACGGTGCGAGGGCGATGCCAAGAACCCAGTGGAAGCCGTCTTCTAGCTTGA
Protein Sequence
  • >LOC_Os02g28870.1
    MDPEAEEAQLRLEMELAKKAKADMSGLQRSSSLGLDHAGLYPLPLPPGWRSAPTSPLRTPSSPPPLQFPPAWAADVAGTSGSAAPEDDGPARNAGADEATAGSAPKNEDPARAAGADDGPTRSDYAAMMRMALAKFQDDDAAADDEEAASAVMEQAMTGLMDLTYRKAKPPELPYEFATRWPIPIAHDGTLQAEVMRDPVILPSGYSVDQTYQNNQKRQNPWTNTSTFTDHSLPYSLSVPNHLLRDMISAWCLDHSDLSPSTTSDTPSTPLEPSEEEQIQRILKLFSGNSASQREALKLIQLLTKTTKGVQPCLAKYADIIPVLINLRRKYKSSWTQDLEEERLTIILNLTMHRQNREILAGQNELAGAIKKIVKKAGNRGKRTSSLAKVASIVAVLSEFDMFRKRMLDAGGMKMLRGMLKIKDTEVITEAATAILALYADGEGEQPARFHEVPQMLLECHMFTDGILLLLDRLPKSPRVFRKICDQALQLVNIVMAEDASGPVTRKGILSAISLIYEIVERDVGKMNAVKNMEDFIERLRQLSSDRLPMQKMLQVERIIRTLSDAFPAPTVRGRCQEPSGSRLLA*