Gene Details:

Functional Descriptions:

  • In the present study, we identified six rice NAAT genes (OsNAAT1-6) by screening a cDNA library made from Fe-deficient rice roots and by searching databases.
  • Northern blot and RT-PCR analysis showed that OsNAAT1, but not OsNAAT2-6, was strongly up-regulated by Fe deficiency, both in roots and shoots.
  • Promoter-GUS analysis revealed that OsNAAT1 was expressed in companion and pericycle cells adjacent to the protoxylem of Fe-sufficient roots.
  • OsNAAT1 expression was also observed in the companion cells of Fe-sufficient shoots, and was clearly induced in all the cells of Fe-deficient leaves.
  • Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os02g20360.1
    AGGCGCGCATGCATGCTTCATGCTGCTGTGCCCCACCCGAGTCCGTGTCCCACACCCGTAGAATCTCCTACAAATATAGCGGCACTAGCTATCCGACGCGCACCACCACCACCTCCTCCTCTGCACCGGAATTCACCAACAAAAAACAGAGCACGGCCATGGCACCGACGACGGCGGCGGCGGCGGCGAGCAGCAACGGCGGCGGCGAGAGCGACGGCAGCAGCAAGGAGTGGAGGCTGACGGCGCCGACGAGGGGCGGCGCGATGGCGGCGGCGGGGGACAAGATGAGCATCCGGGCGGTGCGGTACAAGATCAGCGCCAGCGTCGACGACCGCGGCCCGCGCCCCGTCCTGCCGCTCGCCCACGGCGACCCCTCCGTGTTCCCCGAGTTCCGCACCGCCGCCGAGGCCGAGGACGCCGTCGCCGACGCGCTCCGCTCCGGCGACTTCAACTGCTACCCCGCCGGCGTCGGCCTCCCCGCCGCGCGACGTGCTGTGGCAGATCATTTGTCACGCGACCTCCCATACAAGCTATCTTCTGATGACATCTTCCTAACCGCTGGAGGAACTCAGGCCATCGAGGTCGTAATCTCAATCCTTGCCCAACCTGGCACAAACATATTGCTTCCTAGACCAGGCTACCCAAACTATGAAGCTCGAGCCGCGTTCAACAACCTTGAAGTTCGTCACTTTGATCTTATTCCTGAGAAGGGCTGGGAGATTGACCTTAACTCCCTAGAATCTATTGCGGACAAGAACACTACTGCGATAGTCATCATAAATCCCAATAATCCATGCGGGAATGTGTACACTTACGAGCATTTATCCAAGGTGGCAGAGGTAGCAAGGAAGCTTGGGATATTGGTAATTACTGATGAGGTGTATGGTAATTTGGTTTTTGGGAGTTCCCCATTTGTCCCAATGGGTTGCTTTGGGCACATCGTACCAATATTAACCATAGGATCGCTATCAAAGAGGTGGATAGTGCCGGGATGGCGACTTGGTTGGGTAGCAATATGTGACCCCAAGAAGACTCTACAAGAAACCAAGATTGCAACATTAATTACTAATTTCCTTAATGTTTCAACTGATCCAGCAACTTTCATTCAGGGAGCTCTACCGAATATTCTTAAGAATACCAAGGAAGAATTCTTTAAGAGGATAATTGATTTGCTTACGGAAACATCAGATATTTGCTATAGAGGAATAAAGGATATTAAATGCATCACTTGTCCTCACAAGCCCGAAGGATCCATGTTTGTGATGGTGAAATTGAACCTATATCTTTTGGAGGGAATCCATGATGATGTTGATTTTTGTTGCCAACTTGCGAAAGAAGAGTCGGTGATTCTTTGCCCAGGGAGTGTGCTGGGAATGAAGAATTGGGTTCGCATTACTTTTGCTATTGATTCATCTTCTCTCCTGGATGGTCTTGAGAGGATCAAATCCTTCTGCCAAAGGCACAAGAAGAAAAACCCTTTAAATTATATCTAGATTGTACTAGGATTGGAATGATCAGTACTAATTGTGATGTTTGTGCTTTATACCTGTTGTCACCAAAATGGAACTATTAATTTGCTATGAATAAAGCTTACAAATTCATATGTGTCAATGAACTCATCTATAAAAGTTGCACATGTAGTTTACATAGACT
  • >LOC_Os02g20360.2
    AGGCGCGCATGCATGCTTCATGCTGCTGTGCCCCACCCGAGTCCGTGTCCCACACCCGTAGAATCTCCTACAAATATAGCGGCACTAGCTATCCGACGCGCACCACCACCACCTCCTCCTCTGCACCGGAATTCACCAACAAAAAACAGAGCACGGCCATGGCACCGACGACGGCGGCGGCGGCGGCGAGCAGCAACGGCGGCGGCGAGAGCGACGGCAGCAGCAAGGAGTGGAGGCTGACGGCGCCGACGAGGGGCGGCGCGATGGCGGCGGCGGGGGACAAGATGAGCATCCGGGCGGTGCGGTACAAGATCAGCGCCAGCGTCGACGACCGCGGCCCGCGCCCCGTCCTGCCGCTCGCCCACGGCGACCCCTCCGTGTTCCCCGAGTTCCGCACCGCCGCCGAGGCCGAGGACGCCGTCGCCGACGCGCTCCGCTCCGGCGACTTCAACTGCTACCCCGCCGGCGTCGGCCTCCCCGCCGCGCGACGTGCTGTGGCAGATCATTTGTCACGCGACCTCCCATACAAGCTATCTTCTGATGACATCTTCCTAACCGCTGGAGGAACTCAGGCCATCGAGGTCGTAATCTCAATCCTTGCCCAACCTGGCACAAACATATTGCTTCCTAGACCAGGCTACCCAAACTATGAAGCTCGAGCCGCGTTCAACAACCTTGAAGTTCGTCACTTTGATCTTATTCCTGAGAAGGGCTGGGAGATTGACCTTAACTCCCTAGAATCTATTGCGGACAAGAACACTACTGCGATAGTCATCATAAATCCCAATAATCCATGCGGGAATGTGTACACTTACGAGCATTTATCCAAGGTGGCAGAGGTAGCAAGGAAGCTTGGGATATTGGTAATTACTGATGAGGTGTATGGTAATTTGGTTTTTGGGAGTTCCCCATTTGTCCCAATGGGTTGCTTTGGGCACATCGTACCAATATTAACCATAGGATCGCTATCAAAGAGGTGGATAGTGCCGGGATGGCGACTTGGTTGGGTAGCAATATGTGACCCCAAGAAGACTCTACAAGAAACCAAGATTGCAACATTAATTACTAATTTCCTTAATGTTTCAACTGATCCAGCAACTTTCATTCAGGGAGCTCTACCGAATATTCTTAAGAATACCAAGGAAGAATTCTTTAAGAGGATAATTGATTTGCTTACGGAAACATCAGATATTTGCTATAGAGGAATAAAGGATATTAAATGCATCACTTGTCCTCACAAGCCCGAAGGATCCATGTTTGTGATGGTGAAATTGAACCTATATCTTTTGGAGGGAATCCATGATGATGTTGATTTTTGTTGCCAACTTGCGAAAGAAGAGTCGGTGATTCTTTGCCCAGGGAGTGTGCTGGGAATGAAGAATTGGGTTCGCATTACTTTTGCTATTGATTCATCTTCTCTCCTGGATGGTCTTGAGAGGATCAAATCCTTCTGCCAAAGGCACAAGAAGAAAAACCCTTTAAATTATATCTAGATTGTACTAGGATTGGAATGATCAGTACTAATTGTGATGTTTGTGCTTTATACCTGTTGTCACCAAAATGGAACTATTAATTTGCTATGAATAAAGCTTACAAATTCATATGTGTCAATGAACTCATCTATAAAAGTTGCACATGTAGTTTACATAGACT
CDS Sequence
  • >LOC_Os02g20360.1
    ATGCATGCTTCATGCTGCTGTGCCCCACCCGAGTCCGTGTCCCACACCCGTAGAATCTCCTACAAATATAGCGGCACTAGCTATCCGACGCGCACCACCACCACCTCCTCCTCTGCACCGGAATTCACCAACAAAAAACAGAGCACGGCCATGGCACCGACGACGGCGGCGGCGGCGGCGAGCAGCAACGGCGGCGGCGAGAGCGACGGCAGCAGCAAGGAGTGGAGGCTGACGGCGCCGACGAGGGGCGGCGCGATGGCGGCGGCGGGGGACAAGATGAGCATCCGGGCGGTGCGGTACAAGATCAGCGCCAGCGTCGACGACCGCGGCCCGCGCCCCGTCCTGCCGCTCGCCCACGGCGACCCCTCCGTGTTCCCCGAGTTCCGCACCGCCGCCGAGGCCGAGGACGCCGTCGCCGACGCGCTCCGCTCCGGCGACTTCAACTGCTACCCCGCCGGCGTCGGCCTCCCCGCCGCGCGACGTGCTGTGGCAGATCATTTGTCACGCGACCTCCCATACAAGCTATCTTCTGATGACATCTTCCTAACCGCTGGAGGAACTCAGGCCATCGAGGTCGTAATCTCAATCCTTGCCCAACCTGGCACAAACATATTGCTTCCTAGACCAGGCTACCCAAACTATGAAGCTCGAGCCGCGTTCAACAACCTTGAAGTTCGTCACTTTGATCTTATTCCTGAGAAGGGCTGGGAGATTGACCTTAACTCCCTAGAATCTATTGCGGACAAGAACACTACTGCGATAGTCATCATAAATCCCAATAATCCATGCGGGAATGTGTACACTTACGAGCATTTATCCAAGGTGGCAGAGGTAGCAAGGAAGCTTGGGATATTGGTAATTACTGATGAGGTGTATGGTAATTTGGTTTTTGGGAGTTCCCCATTTGTCCCAATGGGTTGCTTTGGGCACATCGTACCAATATTAACCATAGGATCGCTATCAAAGAGGTGGATAGTGCCGGGATGGCGACTTGGTTGGGTAGCAATATGTGACCCCAAGAAGACTCTACAAGAAACCAAGATTGCAACATTAATTACTAATTTCCTTAATGTTTCAACTGATCCAGCAACTTTCATTCAGGGAGCTCTACCGAATATTCTTAAGAATACCAAGGAAGAATTCTTTAAGAGGATAATTGATTTGCTTACGGAAACATCAGATATTTGCTATAGAGGAATAAAGGATATTAAATGCATCACTTGTCCTCACAAGCCCGAAGGATCCATGTTTGTGATGGTGAAATTGAACCTATATCTTTTGGAGGGAATCCATGATGATGTTGATTTTTGTTGCCAACTTGCGAAAGAAGAGTCGGTGATTCTTTGCCCAGGGAGTGTGCTGGGAATGAAGAATTGGGTTCGCATTACTTTTGCTATTGATTCATCTTCTCTCCTGGATGGTCTTGAGAGGATCAAATCCTTCTGCCAAAGGCACAAGAAGAAAAACCCTTTAAATTATATCTAG
  • >LOC_Os02g20360.2
    ATGCATGCTTCATGCTGCTGTGCCCCACCCGAGTCCGTGTCCCACACCCGTAGAATCTCCTACAAATATAGCGGCACTAGCTATCCGACGCGCACCACCACCACCTCCTCCTCTGCACCGGAATTCACCAACAAAAAACAGAGCACGGCCATGGCACCGACGACGGCGGCGGCGGCGGCGAGCAGCAACGGCGGCGGCGAGAGCGACGGCAGCAGCAAGGAGTGGAGGCTGACGGCGCCGACGAGGGGCGGCGCGATGGCGGCGGCGGGGGACAAGATGAGCATCCGGGCGGTGCGGTACAAGATCAGCGCCAGCGTCGACGACCGCGGCCCGCGCCCCGTCCTGCCGCTCGCCCACGGCGACCCCTCCGTGTTCCCCGAGTTCCGCACCGCCGCCGAGGCCGAGGACGCCGTCGCCGACGCGCTCCGCTCCGGCGACTTCAACTGCTACCCCGCCGGCGTCGGCCTCCCCGCCGCGCGACGTGCTGTGGCAGATCATTTGTCACGCGACCTCCCATACAAGCTATCTTCTGATGACATCTTCCTAACCGCTGGAGGAACTCAGGCCATCGAGGTCGTAATCTCAATCCTTGCCCAACCTGGCACAAACATATTGCTTCCTAGACCAGGCTACCCAAACTATGAAGCTCGAGCCGCGTTCAACAACCTTGAAGTTCGTCACTTTGATCTTATTCCTGAGAAGGGCTGGGAGATTGACCTTAACTCCCTAGAATCTATTGCGGACAAGAACACTACTGCGATAGTCATCATAAATCCCAATAATCCATGCGGGAATGTGTACACTTACGAGCATTTATCCAAGGTGGCAGAGGTAGCAAGGAAGCTTGGGATATTGGTAATTACTGATGAGGTGTATGGTAATTTGGTTTTTGGGAGTTCCCCATTTGTCCCAATGGGTTGCTTTGGGCACATCGTACCAATATTAACCATAGGATCGCTATCAAAGAGGTGGATAGTGCCGGGATGGCGACTTGGTTGGGTAGCAATATGTGACCCCAAGAAGACTCTACAAGAAACCAAGATTGCAACATTAATTACTAATTTCCTTAATGTTTCAACTGATCCAGCAACTTTCATTCAGGGAGCTCTACCGAATATTCTTAAGAATACCAAGGAAGAATTCTTTAAGAGGATAATTGATTTGCTTACGGAAACATCAGATATTTGCTATAGAGGAATAAAGGATATTAAATGCATCACTTGTCCTCACAAGCCCGAAGGATCCATGTTTGTGATGGTGAAATTGAACCTATATCTTTTGGAGGGAATCCATGATGATGTTGATTTTTGTTGCCAACTTGCGAAAGAAGAGTCGGTGATTCTTTGCCCAGGGAGTGTGCTGGGAATGAAGAATTGGGTTCGCATTACTTTTGCTATTGATTCATCTTCTCTCCTGGATGGTCTTGAGAGGATCAAATCCTTCTGCCAAAGGCACAAGAAGAAAAACCCTTTAAATTATATCTAG
Protein Sequence
  • >LOC_Os02g20360.1
    MHASCCCAPPESVSHTRRISYKYSGTSYPTRTTTTSSSAPEFTNKKQSTAMAPTTAAAAASSNGGGESDGSSKEWRLTAPTRGGAMAAAGDKMSIRAVRYKISASVDDRGPRPVLPLAHGDPSVFPEFRTAAEAEDAVADALRSGDFNCYPAGVGLPAARRAVADHLSRDLPYKLSSDDIFLTAGGTQAIEVVISILAQPGTNILLPRPGYPNYEARAAFNNLEVRHFDLIPEKGWEIDLNSLESIADKNTTAIVIINPNNPCGNVYTYEHLSKVAEVARKLGILVITDEVYGNLVFGSSPFVPMGCFGHIVPILTIGSLSKRWIVPGWRLGWVAICDPKKTLQETKIATLITNFLNVSTDPATFIQGALPNILKNTKEEFFKRIIDLLTETSDICYRGIKDIKCITCPHKPEGSMFVMVKLNLYLLEGIHDDVDFCCQLAKEESVILCPGSVLGMKNWVRITFAIDSSSLLDGLERIKSFCQRHKKKNPLNYI*
  • >LOC_Os02g20360.2
    MHASCCCAPPESVSHTRRISYKYSGTSYPTRTTTTSSSAPEFTNKKQSTAMAPTTAAAAASSNGGGESDGSSKEWRLTAPTRGGAMAAAGDKMSIRAVRYKISASVDDRGPRPVLPLAHGDPSVFPEFRTAAEAEDAVADALRSGDFNCYPAGVGLPAARRAVADHLSRDLPYKLSSDDIFLTAGGTQAIEVVISILAQPGTNILLPRPGYPNYEARAAFNNLEVRHFDLIPEKGWEIDLNSLESIADKNTTAIVIINPNNPCGNVYTYEHLSKVAEVARKLGILVITDEVYGNLVFGSSPFVPMGCFGHIVPILTIGSLSKRWIVPGWRLGWVAICDPKKTLQETKIATLITNFLNVSTDPATFIQGALPNILKNTKEEFFKRIIDLLTETSDICYRGIKDIKCITCPHKPEGSMFVMVKLNLYLLEGIHDDVDFCCQLAKEESVILCPGSVLGMKNWVRITFAIDSSSLLDGLERIKSFCQRHKKKNPLNYI*