Information report for OsCBL8
Gene Details
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Functional Descriptions
- By evaluation of the tolerance to 150 mmol/L NaCl, 20% PEG6000 and low temperature treatments, and relevant physiological indices, 8F12, a sense transgenic line with high salt tolerance, and 8R14, an antisense transgenic line with high drought tolerance, were obtained, which suggests that the OsCBL8 gene is involved in the response of rice to abiotic stresses.
- The OsCBL8 transgenic rice seedlings showed more tolerance to salt stress than non-transgenic seedlings.
- Such up- and down-regulation of the OsCBL8 gene in these transgenic lines had little effects on main agronomic traits, but significantly decreased the number of filled grains per panicle and seed setting rate in some of transgenic lines.
- Moreover, Calcineurin B-like protein 7 (OsCBL7) and OsCBL8 interact with OsCIPK7 specifically on the plasma membrane.
- This study found that OsCBL8 negatively regulated rice seed germination and seedling growth by interacting with OsCIPK17 with overexpression and gene editing mutant plants as materials combining plant phenotype, physiological indicators and transcriptome sequencing.
- Our work reveals a unique signaling pathway, wherein OsCBL8 interacts with OsCIPK17 and provides rice with multiple resistance while also regulating seedling growth.
Functional Keywords
- drought , salt-stress , seed , grains-per-panicle , temperature , salt , abiotic-stress , drought-tolerance , grain , salt-tolerance , seedling , panicle , plasma-membrane , growth , resistance , seed-germination , seedling-growth
Literature and News
- Expression analysis of the calcineurin B-like gene family in rice (Oryza sativa L.) under environmental stresses . DOI: 10.1016/j.gene.2008.02.011 ; PMID: 18395997
- Preliminary Study on Function of Calcineurin B-Like Protein Gene OsCBL8 in Rice . DOI: 10.1016/s1672-6308(08)60099-2
- Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice . DOI: 10.15252/embj.2021110518 ; PMID: 36341575
- The OsCBL8-OsCIPK17 Module Regulates Seedling Growth and Confers Resistance to Heat and Drought in Rice . DOI: 10.3390/ijms232012451 ; PMID: 36293306
Gene Resources
- UniProt: Q3HRN9
- EMBL: AP004229, AP014958, DQ201202
- AlphaFoldDB: Q3HRN9
- EnsemblPlants: Os02t0291400-01
- Gramene: Os02t0291400-01
- KEGG: osa:107276173
- Orthologous matrix: CQQRCKK
- InterPro: IPR002048, IPR011992, IPR018247
- PANTHER: PTHR23056, PTHR23056:SF137
- SUPFAM: SSF47473
- PROSITE: PS00018, PS50222
- Gene3D: 1.10.238.10
- OrthoDB: Q3HRN9
- SWISS-MODEL: Q3HRN9
- Conserved Domain Database: cd00051
- eggNOG: KOG0034
Homologs
- Gossypium hirsutum: GhCBL2
- Lactuca sativa: Lsat_1_v5_gn_8_117641
- Malus domestica: MdCBL1
- Panicum virgatum: Pavir.1KG084500
- Triticum aestivum: TaCBL4
Sequences
cDNA Sequence
- >LOC_Os02g18930.1
ATGGGCTGTGTATCATCAAAGCAGTTCAAAAGAGCTGCACAACATGAGGATCCTGCTATTCTGGCCAAAGAGACTACATTTTCAGTGAGTGAAGTGGAGGCCCTCTTTGAGCTGTTCAAGAAGATAAGCCATTCCATATTCAGAGATGGTCTCATTCACAAGGAGGAGTTCCAGCTAGCTCTCTTCAGGAACAGCAACAAGAAGAACCTTTTCGCCAATCGGATATTTGATCTCTTTGATCTGAAGCGCAACGGTGTTATCGATTTCGGGGAGTTCGTTCGATCCCTCAGCATTTTTCACCCGGAAACGCCTTTGGGAGACAAGATTGCCTTTGCATTTAGGTTGTATGACCTGAGAGGAACTGGATGCATTGAACGTGAAGAGTTGCACGAAATGGTGCTTGCTCTTCTGAATGAATCAGATCTATTCCTATCTGAAGAAGCTGTAGAACAAATCGTGGATCAGACGTTCAAGCAAGCAGACTTAAACGATGACGGGAAGATCGACCCCGATGAATGGAAAACGTTTGCGAGTAAAAACCCGGCTTTGCTGAAGAACATGACTCTTCCATACCTAAAGGACATAACCATGGTATTCCCCAGCTTTATTCTGAACTCTGAAGTTTGTGAGGAAGAGTTGTAA
CDS Sequence
- >LOC_Os02g18930.1
ATGGGCTGTGTATCATCAAAGCAGTTCAAAAGAGCTGCACAACATGAGGATCCTGCTATTCTGGCCAAAGAGACTACATTTTCAGTGAGTGAAGTGGAGGCCCTCTTTGAGCTGTTCAAGAAGATAAGCCATTCCATATTCAGAGATGGTCTCATTCACAAGGAGGAGTTCCAGCTAGCTCTCTTCAGGAACAGCAACAAGAAGAACCTTTTCGCCAATCGGATATTTGATCTCTTTGATCTGAAGCGCAACGGTGTTATCGATTTCGGGGAGTTCGTTCGATCCCTCAGCATTTTTCACCCGGAAACGCCTTTGGGAGACAAGATTGCCTTTGCATTTAGGTTGTATGACCTGAGAGGAACTGGATGCATTGAACGTGAAGAGTTGCACGAAATGGTGCTTGCTCTTCTGAATGAATCAGATCTATTCCTATCTGAAGAAGCTGTAGAACAAATCGTGGATCAGACGTTCAAGCAAGCAGACTTAAACGATGACGGGAAGATCGACCCCGATGAATGGAAAACGTTTGCGAGTAAAAACCCGGCTTTGCTGAAGAACATGACTCTTCCATACCTAAAGGACATAACCATGGTATTCCCCAGCTTTATTCTGAACTCTGAAGTTTGTGAGGAAGAGTTGTAA
Protein Sequence
- >LOC_Os02g18930.1
MGCVSSKQFKRAAQHEDPAILAKETTFSVSEVEALFELFKKISHSIFRDGLIHKEEFQLALFRNSNKKNLFANRIFDLFDLKRNGVIDFGEFVRSLSIFHPETPLGDKIAFAFRLYDLRGTGCIEREELHEMVLALLNESDLFLSEEAVEQIVDQTFKQADLNDDGKIDPDEWKTFASKNPALLKNMTLPYLKDITMVFPSFILNSEVCEEEL*