Information report for EP3;LP
Gene Details
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Functional Descriptions
- Genetic analysis revealed that the erect panicle phenotype was controlled by a single recessive mutation designated erect panicle 3 (EP3).
- A line carrying a T-DNA insertion in LOC_ Os02g15950 was obtained and shown to have the same phenotype as the EP3 mutant, thus confirming the identification of LOC_Os02g15950 as the ERECT PANICLE 3 (EP3) gene.
- The EP3 mutation causes a significant increase in the number of small vascular bundles as well as the thickness of parenchyma in the peduncle, which results in the erect panicle phenotype.
- We report herein an in-depth characterization of two allelic larger panicle (LP) mutants that show significantly increased panicle size as well as improved plant architecture.
- These results suggest that LP plays an important role in regulating plant architecture, particularly in regulating panicle architecture, thereby representing promising targets for genetic improvement of grain production plants.
- Quantitative real-time PCR results show that OsCKX2, which encodes cytokinin oxidase/dehydrogenase, is down-regulated evidently in mutants, implying that LP might be involved in modulating cytokinin level in plant tissues.
- Map-based cloning reveals that LARGER PANICLE (LP) encodes a Kelch repeat-containing F-box protein.
- This anatomical characteristic may account for the observed decrease in leaf photosynthesis and provides evidence that EP3 plays a role in regulating stomatal guard cell development.
- Ectopic expression of the Os02g15950 coding sequence, driven by the HWS (At3g61950) promoter, rescued the hws-1 flower phenotype in Arabidopsis confirming that EP3 is a functional orthologue of HWS.
- However, both EP3 mutant plants and transgenic lines that have a T-DNA insertion within the Os02g15950 (EP3) gene exhibit smaller stomatal guard cells compared with their wild-type controls.
- We have identified the putative orthologue genes of the rice microRNA pathway for ORYZA SATIVA DAWDLE (OsDDL) and ORYZA SATIVA SERRATE (OsSE), and demonstrated that EP3 and OsFBK1 affect their transcript levels as well as those of CROWN ROOT DEFECT 1/ORYZA SATIVA Exportin-5 HASTY (CRD1/OsHST), ORYZA SATIVA DICER-LIKE 1 (OsDCL) and ORYZA SATIVA WEAVY LEAF1 (OsWAF1).
- The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets.
- Loss, reduction or gain of function lines for EP3 and OsFBK1, show that EP3 and OsFBK1 affect plant architecture, organ size, floral organ number and size, floral morphology, pollen viability, grain size and weight.
Functional Keywords
- erect , vascular-bundle , architecture , panicle-size , panicle-architecture , cytokinin , panicle , grain , leaf , flower , photosynthesis , stomatal , development , root , pollen , grain-size , floral , floral-organ , flower-development , crown , crown-root , plant-architecture , organ-size , floral-organ-number
Literature and News
- Map-based cloning of the ERECT PANICLE 3 gene in rice . DOI: 10.1007/s00122-009-1151-x ; PMID: 19756471
- Mutations in the F-box gene LARGER PANICLE improve the panicle architecture and enhance the grain yield in rice . DOI: 10.1111/j.1467-7652.2011.00610.x ; PMID: 21447055
- Decreased photosynthesis in the erect panicle 3 ep3 mutant of rice is associated with reduced stomatal conductance and attenuated guard cell development . DOI: 10.1093/jxb/eru525 ; PMID: 25582452
- The rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets . DOI: 10.1111/pbi.13710 ; PMID: 34543503
Gene Resources
- UniProt: G3CKN6
- EMBL: HQ018816
- AlphaFoldDB: G3CKN6
- EnsemblPlants: Os02t0260200-01
- Gramene: Os02t0260200-01
- KEGG: osa:4328935
- InterPro: IPR001810, IPR011043, IPR015915
- PANTHER: PTHR10706, PTHR10706:SF144
- SUPFAM: SSF50965, SSF81383
- PROSITE: PS50181
- Gene3D: 1.20.1280.50, 2.120.10.80
- OrthoDB: G3CKN6
- Conserved Domain Database: cd09917
Sequences
cDNA Sequence
- >LOC_Os02g15950.1
AGGGACCAAAATGCCCCTTCCTTCTCTTCTTCTTCCCTCACCTCCTCCCCCTCTTTCCCTCTCAGCTCCCTCCATCTCTCTCTCCCCTCTCTCTCTCTCTCTCTCTCTCTCTGATCTGCAGAGCCGGAGCCCGGAGGCGCGGCTGCGGCTGCCAAGCCTCCTCCTCCTCCTCCAGCGCGCGGCCAGCGAGCAGCCTCGGAGGCCACCGCCGCCGCCTCCTCGCCCGGACGGCGGGGGAGCGGGCGGTGCTCCGTCCAGCGGAGCGAGCCCCCCCAATCTTGGCCGGAATCGGATTGAAGGATCTTGCGTTAGTTACTGTGCTGTTCGCTGGATGCTGGCAATGGGGTCAGAGGAGTGGGAGTTGTATCCATCTTCCTTCATTGGTGCTCAGGTCATAGATTATGGGCATGTTTCTGGAGACATGGATGATGATCAGAGTGGAGACTTGGCCGTGTCGATGGACGCCGTTCTCCCTGATGATCTTTTAGAAAAGGTCCTCTCCTTCTTGCCTGTTGCAAGTGTCATAAGATCTGGATCTGTTTGCAAGAGATGGCATGAGATTGTGCATGCCCGGAGGCAGACATGGAGCAAAATGGTGCCTCAGAAGCCTTGGTACTTCATGTTTACCTGCAGCGAGGAAGCGGTCTCGGGGTTCACCTATGACCCGAGCCTGCGTAAGTGGTATGGATTCGACTTCCCTTGCATTGAGAAGACCACCTGGTCGATTTCCTCATCGTCCGGGTTGGTGTGTCTGATGGACAGTGAGGATAGGAGCCGCATCATCGTGTGCAACCCAATAACTAAGGACTGGAAGAGGCTTGTTGATGCTCCTGGTGGCAAGTCGGCTGATTACAGTGCTCTCGCCATTTCTGTGACCAGGACCTCTCATCAGTACATGGTGGCTGTTGCAAGGTGCAACCAGGTGCCATCAGAGTATTATCAATGGGAGTTCACCATCCATTTGTACGAGTCAGAGATAAATACATGGGTGTCTCCCTTCACTGAACTATTGATTGGATGGCGAGGAGGTGATGAGTGCGTGATCTGTGATGGGGTCCTCTACTATCTGGTGTACTCAACAGGAGTTTTGGTGAATAACAACGAGCATCGCCATTGTCTTCTCATGTATGACCTATCTACCAGGCCTACTCACACTTCTTTGATGAGCATGGCTATACCAGTGCCGTGCCCTCTTACATGTGGCCGTTTGATGAACCTCAATGAGAGGCTTGTCTTGGTTGGGGGCATTGGCAAGCAAGATAGGCCTGGTATCATCAAGGGAATTGGCATCTGGGAGCTCCGGAACAAGGAGTGGCATGAGGTTGCTCGAATGCCTCACAAGTTTTTCCAAGGATTTGGCGAGTTCGATGATGTTTTTGCAAGCTGTGGGGCGGATGACCTTATCTATATTCAGAGCTATGGGTCACCGGCCCTCCTCACATTTGAATTAAACCAGAAGCTGTGGAAATGGTCACTGAAGAGCCCTGTGACGAAGAGGTTTCCTCTGCAGCTGTTTACTGGTTTCTCTTTTGAGCCTAGGCTGGACATTGCTTCCTAGTTTTATTTTGTTTCACTTCCACGGGAATTCTTGCATTATTTGCCTGGTTTGGAGTTACCAAGTGAAGAGATGATGTCATTGGAAGCCATTCGAGAAAGTGTTTCAGGTTTTATAGATCTATTCTATTTATTGCTGAGTATATCTTCTAATTGAAAGGGCGTCTAAACTTCCCTGTTTCATTTTTGTTGATTTGATGGTTGGATAATAGAGGGAGGGATGATGCCATACACAAGAACAATTCTTGCTGAATTGTGTTTTCTTTATTCTTATGTTGCCTGCAAACAAAGTGCCACTTCAGATGTCCCAAACCTAAAAGATTGTTACTTTCTTGATTTATAGTAGTCATTTGCGTTTGGGGTCCCCGCTGCTACTGATTTATGTTTTGTTTTATTACGACACCACTACATGCAAACATCTGAACTTGAAGCAGAGTCCTTTGTTGTTGAGTCAGTACTTGAAGCAGAGTCCTTGCTCTGCTTGCCGTATAACCATTGATGGACAGCAGAGCTGTTAAGGTTGCCTTCTCTTTTAGTCCAATGCACAAAATCAATCTGTCCATCACTCTTATTAGCACTTACATAATCAGAATTTTTGGAAATGCAGATAGTTAACTGAAAATAAAAACATGGATATCAAACCATGCCATGTAAATCGCGAAGAGCTGGTTTATTGAAATATTAGGCAAACATTTGGTGATCTCTGGTTTTACTGTACTCATTGTTTATCCCTAATGTTCTTATACTTATCTCCTCTGTCAAGTTCCTTTGATGCTATTTGAAGCTCATGTTTATCCATAATGTTCTTCTACTTATGTCCTATAGTACTAAACTACTAACTGCTAAACTGTACCATACTTTATCCCAATTACTCATACAGCATTGTGCTGTTCATGTAGTGCCAGAATCCTTGCCTGCTTCTGATGCAGCATCTGTGTCATACTACATAGCAATGGCATGTGATTGTGCAAAGACAGGTAAAGGAGCATGCATGCTGCATCAGGGACTCCCGGACTCAGAGTCTCTCTTTGGTTTTGGATGGATACGTTAACACTATTTTCCTCATGCCTGAACTCTCTGTACTTGCTTGATAGTACCACCAATCCTAAAAAGCAGCATCTCCCTTTTTATATTTTCTTGCCTGCTGTCACTGCTCCCAAATGTTGCTTAAAGCCTGTAAAGCCACACCCTCTGCTCTGCAAGTGAAGGACACATATTGTTTCAGATGTCCAGCTGTCAATGATGTATGTTGTGTATGCCTTCAGAGAATCAGAAAGAGACCCTTTCAGCCAGCCAGCAGGTGAGCTCAGTGATGTGGTACCAGCAGGGTTGCATTGTTGCAAGCATGGGGCAATTTCTTCTGATGAGCATATATATTGATGTTGAAGGGTGGTGTATGATATCTTGGGGACAGTTTCTACCAGTCTGTTATACTACTAGAAAAGGTAGTAAATGTGGGTGTGTATGTAATTGTCACTTTGCAAAGGTGTCCTCCAAATGCATTTGCTGTGCAAGTATTGTCCAATGCTGTACCTGAACTTGTTGAGCTTTGTGCTATAGTGATTTTTATTAGCAGAAAGCATGGTGTTCTGTCAAAGGATATCCCACAACTTGAACATGATCAGTGCTTAGC
CDS Sequence
- >LOC_Os02g15950.1
ATGCCCCTTCCTTCTCTTCTTCTTCCCTCACCTCCTCCCCCTCTTTCCCTCTCAGCTCCCTCCATCTCTCTCTCCCCTCTCTCTCTCTCTCTCTCTCTCTCTGATCTGCAGAGCCGGAGCCCGGAGGCGCGGCTGCGGCTGCCAAGCCTCCTCCTCCTCCTCCAGCGCGCGGCCAGCGAGCAGCCTCGGAGGCCACCGCCGCCGCCTCCTCGCCCGGACGGCGGGGGAGCGGGCGGTGCTCCGTCCAGCGGAGCGAGCCCCCCCAATCTTGGCCGGAATCGGATTGAAGGATCTTGCGTTAGTTACTGTGCTGTTCGCTGGATGCTGGCAATGGGGTCAGAGGAGTGGGAGTTGTATCCATCTTCCTTCATTGGTGCTCAGGTCATAGATTATGGGCATGTTTCTGGAGACATGGATGATGATCAGAGTGGAGACTTGGCCGTGTCGATGGACGCCGTTCTCCCTGATGATCTTTTAGAAAAGGTCCTCTCCTTCTTGCCTGTTGCAAGTGTCATAAGATCTGGATCTGTTTGCAAGAGATGGCATGAGATTGTGCATGCCCGGAGGCAGACATGGAGCAAAATGGTGCCTCAGAAGCCTTGGTACTTCATGTTTACCTGCAGCGAGGAAGCGGTCTCGGGGTTCACCTATGACCCGAGCCTGCGTAAGTGGTATGGATTCGACTTCCCTTGCATTGAGAAGACCACCTGGTCGATTTCCTCATCGTCCGGGTTGGTGTGTCTGATGGACAGTGAGGATAGGAGCCGCATCATCGTGTGCAACCCAATAACTAAGGACTGGAAGAGGCTTGTTGATGCTCCTGGTGGCAAGTCGGCTGATTACAGTGCTCTCGCCATTTCTGTGACCAGGACCTCTCATCAGTACATGGTGGCTGTTGCAAGGTGCAACCAGGTGCCATCAGAGTATTATCAATGGGAGTTCACCATCCATTTGTACGAGTCAGAGATAAATACATGGGTGTCTCCCTTCACTGAACTATTGATTGGATGGCGAGGAGGTGATGAGTGCGTGATCTGTGATGGGGTCCTCTACTATCTGGTGTACTCAACAGGAGTTTTGGTGAATAACAACGAGCATCGCCATTGTCTTCTCATGTATGACCTATCTACCAGGCCTACTCACACTTCTTTGATGAGCATGGCTATACCAGTGCCGTGCCCTCTTACATGTGGCCGTTTGATGAACCTCAATGAGAGGCTTGTCTTGGTTGGGGGCATTGGCAAGCAAGATAGGCCTGGTATCATCAAGGGAATTGGCATCTGGGAGCTCCGGAACAAGGAGTGGCATGAGGTTGCTCGAATGCCTCACAAGTTTTTCCAAGGATTTGGCGAGTTCGATGATGTTTTTGCAAGCTGTGGGGCGGATGACCTTATCTATATTCAGAGCTATGGGTCACCGGCCCTCCTCACATTTGAATTAAACCAGAAGCTGTGGAAATGGTCACTGAAGAGCCCTGTGACGAAGAGGTTTCCTCTGCAGCTGTTTACTGGTTTCTCTTTTGAGCCTAGGCTGGACATTGCTTCCTAG
Protein Sequence
- >LOC_Os02g15950.1
MPLPSLLLPSPPPPLSLSAPSISLSPLSLSLSLSDLQSRSPEARLRLPSLLLLLQRAASEQPRRPPPPPPRPDGGGAGGAPSSGASPPNLGRNRIEGSCVSYCAVRWMLAMGSEEWELYPSSFIGAQVIDYGHVSGDMDDDQSGDLAVSMDAVLPDDLLEKVLSFLPVASVIRSGSVCKRWHEIVHARRQTWSKMVPQKPWYFMFTCSEEAVSGFTYDPSLRKWYGFDFPCIEKTTWSISSSSGLVCLMDSEDRSRIIVCNPITKDWKRLVDAPGGKSADYSALAISVTRTSHQYMVAVARCNQVPSEYYQWEFTIHLYESEINTWVSPFTELLIGWRGGDECVICDGVLYYLVYSTGVLVNNNEHRHCLLMYDLSTRPTHTSLMSMAIPVPCPLTCGRLMNLNERLVLVGGIGKQDRPGIIKGIGIWELRNKEWHEVARMPHKFFQGFGEFDDVFASCGADDLIYIQSYGSPALLTFELNQKLWKWSLKSPVTKRFPLQLFTGFSFEPRLDIAS*