Gene Details:

Functional Descriptions:

  • Docs1 mRNA was expressed in all tissues including roots, leaf blades and sheaths, and flowers.
  • In this work, we report on defective in outer cell layer specification 1 (Docs1), which is involved in the specification of outer cell layers in rice roots.
  • Docs1 was isolated by map-based cloning using a mutant (c68) defective in the outer cell layers of primary roots.
  • Immunostaining with an anti-Docs1 antibody showed that Docs1 was localized at the epidermis and exodermis, depending on the root region.
  • These results suggest that Docs1 might directly or indirectly regulate multiple genes involved in the proper development of root outer cell layers in rice.
  • All these observations add new insights into the Docs1 gene function in gravitropic responses at several stages of plant development.
  • Root cone angle is enlarged in Docs1 LRR-RLK mutants in rice.
  • Besides the aluminum sensitivity phenotype, both Docs1 mutants shared also several root phenotypes described previously: less root hairs and mixed identities of the outer cell layers.
  • Moreover, our new results suggest that Docs1 could also play a role in root cap development.
  • We hypothesized these Docs1 root phenotypes may affect gravity responses.
  • Furthermore, at adult stage, the root gravitropic set angle of Docs1 mutants was also affected since Docs1 mutant plants displayed larger root cone angles.
  • The c68 (Docs1-1) mutant possessed a nonsense mutation in the C-terminal part of the Docs1 kinase domain.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os02g14120.1
    AGTATTATACTCGCGCAGGCGCCCATGGCCGCCAAGCCCGAGCTCCTGCTGCAGCGTAGCCCACCTCAAGCCACCGCCTCCGCCTCCGCCGCCGCTGCTGCGCTGCGCCCGCGCGGCCTCCTCCTCGCCCTCCCCGGCGTCGCCGTCGGACTCCTCTCATGGCGGGGCCCCGCGCCCTCGCCGTGGCGGCGGCGGTGGTGGTCGCCGCGTGGGCCGTCGCCGCCGCCGGGGACCCGCCGCTGTCGCCCAAGGGGCTCAACTACGAAGTGGCGGCGCTGATGGCGGTGAAGAGCAGGATGCGGGACGAGAAGGGGGTGATGGGAGGGTGGGACATCAACTCCGTCGACCCGTGCACCTGGTCCATGGTCGCCTGCTCCCCCGACGGATTCGTCGTCTCGCTGCAGATGGCGAACAACGGATTGGCCGGGACGCTGTCGCCGAGCATCGGGAACCTCAGCCACCTGCAGACAATGTTACTACAGAATAACATGATATCAGGTGGCATTCCTCCAGAGATTGGGAAGTTGACCAATCTGAAAGCTCTTGATCTTTCTGGTAACCAGTTTGTTGGTGAAATCCCAAGTTCTCTAGGGCGGCTGACTGAATTAAACTATCTGCGCCTAGATAAGAACAACTTGTCTGGACAGATCCCTGAAGATGTCGCAAAGCTTCCAGGTCTCACATTCCTTGATTTATCATCCAATAATTTGAGCGGCCCGGTTCCAAAAATCTATGCACATGACTACAGTATTGCAGGAAACAGGTTCCTTTGCAATTCATCAATTATGCATGGTTGCAAAGATCTCACAGTATTAACTAATGAATCAACAATTTCTAGCCCGTCGAAAAAAACAAACAGCCATCATCAACTAGCTCTGGCAATTTCATTAAGCATCATCTGTGCCACAGTATTTGTTCTGTTTGTCATTTGTTGGCTAAAATATTGCAGATGGCGCTTGCCTTTTGCTTCTGCTGACCAAGATCTTGAAATTGAATTGGGCCATCTAAAGCACTTCTCATTTCACGAGCTTCAGAGTGCAACTGACAATTTCAACTCAAAGAACATATTAGGTCAAGGTGGCTTTGGTGTTGTTTATAAAGGCTGTCTGAGAAATGGAGCTTTGGTGGCTGTAAAGAGACTAAAAGATCCTGATATTACTGGTGAAGTTCAATTTCAGACAGAAGTGGAGTTGATTGGTCTAGCTGTGCACAGGAACCTTTTGCGGTTATATGGATTCTGCATGACCTCAAAAGAAAGGTTGCTTGTATATCCATATATGCCAAATGGCAGTGTTGCTGATCGCTTGAGAGATTACCATCATGGAAAACCATCTCTTGATTGGAGCAAGCGTATGCGGATTGCTGTTGGGGCAGCCAGGGGACTGCTGTATCTTCACGAACAATGCAATCCTAAAATCATTCACAGGGATGTTAAAGCAGCTAACATTTTGCTTGATGAAAGTTTTGAAGCAATTGTTGGGGATTTTGGATTGGCAAAACTTCTTGACCGACAAGAATCACATGTTACTACTGCAGTTAGGGGCACTATTGGACATATTGCTCCAGAGTATTTATCAACAGGACAGTCTTCAGAAAAGACCGATGTTTATGGGTTTGGTATTCTCTTGTTGGAACTGATCACTGGGCCTAAAACCTTGAGCAATGGGCACGCGCAATCTCAGAAGGGAATGATTCTAGATTGGGTTAGAGAAGTCAAGGAAGAAAATAAACTGGATAAACTGGTTGACAGGGATCTGAAATATTCATTTGATTTCGCCGAGTTGGAGTGTTCTGTTGACGTAATTCTGCAATGCACTCAGACCAATCCCATTTTGCGTCCCAAGATGTCGGAAGTCCTTAATGCCCTTGAAGCTAATGTCACCCTGCCAGAGAACGGCATCGACTTGAATAGAGAAGTGCCTCCCTATGGAGGATCTTGCAGTTTCTCAGTAAGGCATGAGGATCCTCATGATTCATCATCCTTCATAATAGAGCCAATTGAGCTATCTGGTCCCAGATGATCACAATTGTACATCAGGGGGGAGAACCACAAACAAAAGGATGACAATTGTACAAAAACGTGTATAGTCTGGATGTAGTTTTACTTGGAATCTGCAAGGAGTTTGCTGGTGTTGCAAATGCCTCACGATCCAATCTCTAACTGCTAGGTAGATCGGTCATTTCTGGTCGTGTTGGCGTATGCACTGTACAGTGCAAATCAGTATGCTACCCATCCTACAATATGTTCTCGAGAAAATTACATTGCATCTTGCATGTTTTTCTCAGTTACAAGAATCAGTATGAAAGAGCCCAAAAGCACGAATCAATATGAAATGTTCTTCGCTGAGACGGCTTATTCCTGCGAGTCTACATGCTAAACTCTCATGCTACGTTTACCAAAAAATATTCAGCAGAATACGCCTGCCTCGTCTTGATATCACATACAAAAAAAGCAGCAAATTTTATCTAAATTCTCATGGTCGGTCGGCAGGGCCACACATGACTTGTCAAAATGCTCAGCTCATTTGCATTTATTCTCTTTCACATATCTCTGTTTCTGAGGTGACCTGCCAGGCTGCCACAGGCATAACATCTGACGATTGAAATAACAGGATCGGCATCTATAGCTCTCAAAATTGGGTAGGTTTCTAGTTCTCTCCTCCATACAGGGAAAATCCGAAGTTGGAGAAACACCAGCATGTTCACTGGATTATTTCTCTAGTACAACCATCTTAAAGGTCAATAGACCACCCCCCAAAAAAAAAACGCCGCCGCTTGCAAGCCTCATTTCATTGGCCGAAGAGGGAAGAGGAAGTAGCTTCATTTCGTTTTAGTGACCTCATGAATAGCATTAGCCAAATACGCCACATTTCCTGTTGTTACACCAGCCATGCTGA
CDS Sequence
  • >LOC_Os02g14120.1
    ATGGCGGGGCCCCGCGCCCTCGCCGTGGCGGCGGCGGTGGTGGTCGCCGCGTGGGCCGTCGCCGCCGCCGGGGACCCGCCGCTGTCGCCCAAGGGGCTCAACTACGAAGTGGCGGCGCTGATGGCGGTGAAGAGCAGGATGCGGGACGAGAAGGGGGTGATGGGAGGGTGGGACATCAACTCCGTCGACCCGTGCACCTGGTCCATGGTCGCCTGCTCCCCCGACGGATTCGTCGTCTCGCTGCAGATGGCGAACAACGGATTGGCCGGGACGCTGTCGCCGAGCATCGGGAACCTCAGCCACCTGCAGACAATGTTACTACAGAATAACATGATATCAGGTGGCATTCCTCCAGAGATTGGGAAGTTGACCAATCTGAAAGCTCTTGATCTTTCTGGTAACCAGTTTGTTGGTGAAATCCCAAGTTCTCTAGGGCGGCTGACTGAATTAAACTATCTGCGCCTAGATAAGAACAACTTGTCTGGACAGATCCCTGAAGATGTCGCAAAGCTTCCAGGTCTCACATTCCTTGATTTATCATCCAATAATTTGAGCGGCCCGGTTCCAAAAATCTATGCACATGACTACAGTATTGCAGGAAACAGGTTCCTTTGCAATTCATCAATTATGCATGGTTGCAAAGATCTCACAGTATTAACTAATGAATCAACAATTTCTAGCCCGTCGAAAAAAACAAACAGCCATCATCAACTAGCTCTGGCAATTTCATTAAGCATCATCTGTGCCACAGTATTTGTTCTGTTTGTCATTTGTTGGCTAAAATATTGCAGATGGCGCTTGCCTTTTGCTTCTGCTGACCAAGATCTTGAAATTGAATTGGGCCATCTAAAGCACTTCTCATTTCACGAGCTTCAGAGTGCAACTGACAATTTCAACTCAAAGAACATATTAGGTCAAGGTGGCTTTGGTGTTGTTTATAAAGGCTGTCTGAGAAATGGAGCTTTGGTGGCTGTAAAGAGACTAAAAGATCCTGATATTACTGGTGAAGTTCAATTTCAGACAGAAGTGGAGTTGATTGGTCTAGCTGTGCACAGGAACCTTTTGCGGTTATATGGATTCTGCATGACCTCAAAAGAAAGGTTGCTTGTATATCCATATATGCCAAATGGCAGTGTTGCTGATCGCTTGAGAGATTACCATCATGGAAAACCATCTCTTGATTGGAGCAAGCGTATGCGGATTGCTGTTGGGGCAGCCAGGGGACTGCTGTATCTTCACGAACAATGCAATCCTAAAATCATTCACAGGGATGTTAAAGCAGCTAACATTTTGCTTGATGAAAGTTTTGAAGCAATTGTTGGGGATTTTGGATTGGCAAAACTTCTTGACCGACAAGAATCACATGTTACTACTGCAGTTAGGGGCACTATTGGACATATTGCTCCAGAGTATTTATCAACAGGACAGTCTTCAGAAAAGACCGATGTTTATGGGTTTGGTATTCTCTTGTTGGAACTGATCACTGGGCCTAAAACCTTGAGCAATGGGCACGCGCAATCTCAGAAGGGAATGATTCTAGATTGGGTTAGAGAAGTCAAGGAAGAAAATAAACTGGATAAACTGGTTGACAGGGATCTGAAATATTCATTTGATTTCGCCGAGTTGGAGTGTTCTGTTGACGTAATTCTGCAATGCACTCAGACCAATCCCATTTTGCGTCCCAAGATGTCGGAAGTCCTTAATGCCCTTGAAGCTAATGTCACCCTGCCAGAGAACGGCATCGACTTGAATAGAGAAGTGCCTCCCTATGGAGGATCTTGCAGTTTCTCAGTAAGGCATGAGGATCCTCATGATTCATCATCCTTCATAATAGAGCCAATTGAGCTATCTGGTCCCAGATGA
Protein Sequence
  • >LOC_Os02g14120.1
    MAGPRALAVAAAVVVAAWAVAAAGDPPLSPKGLNYEVAALMAVKSRMRDEKGVMGGWDINSVDPCTWSMVACSPDGFVVSLQMANNGLAGTLSPSIGNLSHLQTMLLQNNMISGGIPPEIGKLTNLKALDLSGNQFVGEIPSSLGRLTELNYLRLDKNNLSGQIPEDVAKLPGLTFLDLSSNNLSGPVPKIYAHDYSIAGNRFLCNSSIMHGCKDLTVLTNESTISSPSKKTNSHHQLALAISLSIICATVFVLFVICWLKYCRWRLPFASADQDLEIELGHLKHFSFHELQSATDNFNSKNILGQGGFGVVYKGCLRNGALVAVKRLKDPDITGEVQFQTEVELIGLAVHRNLLRLYGFCMTSKERLLVYPYMPNGSVADRLRDYHHGKPSLDWSKRMRIAVGAARGLLYLHEQCNPKIIHRDVKAANILLDESFEAIVGDFGLAKLLDRQESHVTTAVRGTIGHIAPEYLSTGQSSEKTDVYGFGILLLELITGPKTLSNGHAQSQKGMILDWVREVKEENKLDKLVDRDLKYSFDFAELECSVDVILQCTQTNPILRPKMSEVLNALEANVTLPENGIDLNREVPPYGGSCSFSVRHEDPHDSSSFIIEPIELSGPR*