Gene Details:

Functional Descriptions:

  • Promoter-GUS analysis of OsCAD2 (pCAD::GUS) in the internode, sheath, and roots revealed that GUS expression was strong in tissues that accumulated high levels of lignin.
  • Consistent with this finding, extracts from roots, internodes, hulls, and panicles of the GH2 plants exhibited drastically reduced CAD activity and undetectable sinapyl alcohol dehydrogenase activity.
  • OsCAD2 is the major CAD gene responsible for monolignol biosynthesis in rice culm.
  • Solid-state nuclear magnetic resonance (NMR) and X-ray diffraction analyses of rice cell walls revealed that and OsCAD2 deficiencies contributed to the disruptions of the cellulose crystalline network.
  • Further, OsCAldOMT1 deficiency contributed to the increase of the cellulose molecular mobility more prominently than OsCAD2 deficiency, resulting in apparently more loosened lignocellulose molecular assembly.
  • A set of rice mutants harboring knockout mutations in either or both OsCAldOMT1 and OsCAD2 was generated in part by genome editing and subjected to comparative cell wall chemical and supramolecular structure analyses.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os02g09490.1
    CTTTAATTATATATACTCGCTGCGGGACGCACCACCCCCTCTCCAGAATTCAGCGAGAGAGAGCGAATCAGCCACTGCACTTCTTGTTCTTGTTCTCTTCCTCCTCCCGAATCCGATCCATCCATTGGCCGCCGCCGCCGCCGCCGCCTCGTGCGTCGCGTGTTCGTGTCCAGAGACGATGGGCAGCCTCGCCGCCGAGAAGACCGTCACCGGGTGGGCCGCCAGGGACGCCTCCGGCCACCTCACCCCCTACAACTACACCCTCAGGAAGACTGGGCCTGAAGATGTGGTGGTGAAGGTTTTGTATTGTGGTATCTGCCATACTGACATCCACCAGGCCAAGAACCACCTTGGTGCTTCCAAGTACCCCATGGTCCCTGGCCATGAGGTGGTCGGCGAGGTGGTGGAGGTCGGGCCGGAGGTGACCAAGTACAGCGCCGGCGACGTCGTCGGCGTCGGCGTCATCGTCGGCTGCTGCCGCGAGTGCCATCCGTGCAAGGCCAATGTGGAGCAGTACTGCAACAAGAGGATTTGGTCCTACAACGACGTCTACACCGACGGCCGGCCAACCCAGGGCGGCTTCGCCTCCGCCATGGTCGTCGACCAGAAGTTTGTGGTGAAGATCCCGGCGGGGCTAGCGCCGGAGCAGGCGGCGCCGCTGCTGTGCGCCGGGCTGACGGTGTACAGCCCACTGAAGCACTTCGGGCTGATGTCGCCAGGTCTCCGCGGCGGCGTCCTGGGGCTCGGCGGCGTGGGGCACATGGGCGTGAAGGTGGCCAAGTCGATGGGGCACCACGTGACGGTGATCAGCTCGTCGGCGAGGAAGCGCGGCGAGGCCATGGACGACCTGGGCGCCGACGCCTACCTCGTCAGCTCCGACGCGGCGGCGATGGCGGCCGCCGGCGACTCGCTGGACTACATCATCGACACCGTGCCGGTGCACCACCCGCTGGAGCCGTACCTGGCGCTGCTGAAGCTGGACGGGAAGCTGATCCTGATGGGGGTGATCAACCAGCCGCTGAGCTTCATCTCGCCCATGGTGATGCTCGGCCGGAAGGCCATCACCGGCAGCTTCATCGGGAGCATGGCCGAGACGGAGGAGGTGCTCAACTTCTGCGTCGACAAGGGGCTCACCTCCCAGATCGAGGTCGTCAAGATGGACTACGTCAACCAGGCCCTCGAGCGCCTCGAGCGCAACGACGTCCGCTACCGCTTCGTCGTCGACGTCGCCGGCAGCAACATCGACGACGCCGACGCGCCGCCCGCCTGATCGCCGCCGTGCACCCTCATGGCAGCATCCGTATGATCCGTTCTTGAACTTGTTTGTGTGAGACTCTGACGACTTGTCCATGTAACGTCCGTGTGCCTTTCCGGTATAAATTCGGCCTCGCAATATATGGACCATTTTGCATTTCCGATCAAAATCATGCTTGCGTTTCACTTGCA
CDS Sequence
  • >LOC_Os02g09490.1
    ATGGGCAGCCTCGCCGCCGAGAAGACCGTCACCGGGTGGGCCGCCAGGGACGCCTCCGGCCACCTCACCCCCTACAACTACACCCTCAGGAAGACTGGGCCTGAAGATGTGGTGGTGAAGGTTTTGTATTGTGGTATCTGCCATACTGACATCCACCAGGCCAAGAACCACCTTGGTGCTTCCAAGTACCCCATGGTCCCTGGCCATGAGGTGGTCGGCGAGGTGGTGGAGGTCGGGCCGGAGGTGACCAAGTACAGCGCCGGCGACGTCGTCGGCGTCGGCGTCATCGTCGGCTGCTGCCGCGAGTGCCATCCGTGCAAGGCCAATGTGGAGCAGTACTGCAACAAGAGGATTTGGTCCTACAACGACGTCTACACCGACGGCCGGCCAACCCAGGGCGGCTTCGCCTCCGCCATGGTCGTCGACCAGAAGTTTGTGGTGAAGATCCCGGCGGGGCTAGCGCCGGAGCAGGCGGCGCCGCTGCTGTGCGCCGGGCTGACGGTGTACAGCCCACTGAAGCACTTCGGGCTGATGTCGCCAGGTCTCCGCGGCGGCGTCCTGGGGCTCGGCGGCGTGGGGCACATGGGCGTGAAGGTGGCCAAGTCGATGGGGCACCACGTGACGGTGATCAGCTCGTCGGCGAGGAAGCGCGGCGAGGCCATGGACGACCTGGGCGCCGACGCCTACCTCGTCAGCTCCGACGCGGCGGCGATGGCGGCCGCCGGCGACTCGCTGGACTACATCATCGACACCGTGCCGGTGCACCACCCGCTGGAGCCGTACCTGGCGCTGCTGAAGCTGGACGGGAAGCTGATCCTGATGGGGGTGATCAACCAGCCGCTGAGCTTCATCTCGCCCATGGTGATGCTCGGCCGGAAGGCCATCACCGGCAGCTTCATCGGGAGCATGGCCGAGACGGAGGAGGTGCTCAACTTCTGCGTCGACAAGGGGCTCACCTCCCAGATCGAGGTCGTCAAGATGGACTACGTCAACCAGGCCCTCGAGCGCCTCGAGCGCAACGACGTCCGCTACCGCTTCGTCGTCGACGTCGCCGGCAGCAACATCGACGACGCCGACGCGCCGCCCGCCTGA
Protein Sequence
  • >LOC_Os02g09490.1
    MGSLAAEKTVTGWAARDASGHLTPYNYTLRKTGPEDVVVKVLYCGICHTDIHQAKNHLGASKYPMVPGHEVVGEVVEVGPEVTKYSAGDVVGVGVIVGCCRECHPCKANVEQYCNKRIWSYNDVYTDGRPTQGGFASAMVVDQKFVVKIPAGLAPEQAAPLLCAGLTVYSPLKHFGLMSPGLRGGVLGLGGVGHMGVKVAKSMGHHVTVISSSARKRGEAMDDLGADAYLVSSDAAAMAAAGDSLDYIIDTVPVHHPLEPYLALLKLDGKLILMGVINQPLSFISPMVMLGRKAITGSFIGSMAETEEVLNFCVDKGLTSQIEVVKMDYVNQALERLERNDVRYRFVVDVAGSNIDDADAPPA*