Information report for GH2;OsCAD2
Gene Details
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Functional Descriptions
- Promoter-GUS analysis of OsCAD2 (pCAD::GUS) in the internode, sheath, and roots revealed that GUS expression was strong in tissues that accumulated high levels of lignin.
- Consistent with this finding, extracts from roots, internodes, hulls, and panicles of the GH2 plants exhibited drastically reduced CAD activity and undetectable sinapyl alcohol dehydrogenase activity.
- OsCAD2 is the major CAD gene responsible for monolignol biosynthesis in rice culm.
- Solid-state nuclear magnetic resonance (NMR) and X-ray diffraction analyses of rice cell walls revealed that and OsCAD2 deficiencies contributed to the disruptions of the cellulose crystalline network.
- Further, OsCAldOMT1 deficiency contributed to the increase of the cellulose molecular mobility more prominently than OsCAD2 deficiency, resulting in apparently more loosened lignocellulose molecular assembly.
- A set of rice mutants harboring knockout mutations in either or both OsCAldOMT1 and OsCAD2 was generated in part by genome editing and subjected to comparative cell wall chemical and supramolecular structure analyses.
Literature and News
- OsCAD2 is the major CAD gene responsible for monolignol biosynthesis in rice culm . DOI: 10.1007/s00299-011-1142-7 ; PMID: 21912859
- Structure of the cinnamyl-alcohol dehydrogenase gene family in rice and promoter activity of a member associated with lignification . DOI: 10.1007/s00425-004-1385-4 ; PMID: 15452707
- GOLD HULL AND INTERNODE2 encodes a primarily multifunctional cinnamyl-alcohol dehydrogenase in rice . DOI: 10.1104/pp.105.073007 ; PMID: 16443696
- Increased lodging resistance in long-culm, low-lignin gh2 rice for improved feed and bioenergy production . DOI: 10.1038/srep06567 ; PMID: 25298209
- Conserved Imprinted Genes between Intra-Subspecies and Inter-Subspecies Are Involved in Energy Metabolism and Seed Development in Rice . DOI: 10.3390/ijms21249618 ; PMID: 33348666
- The OsmiRNA166b-OsHox32 pair regulates mechanical strength of rice plants by modulating cell wall biosynthesis . DOI: 10.1111/pbi.13565 ; PMID: 33560572
- Lignocellulose molecular assembly and deconstruction properties of lignin-altered rice mutants . DOI: 10.1093/plphys/kiac432 ; PMID: 36124989
Gene Resources
- UniProt: Q6ZHS4
- EMBL: AB122054, AK105011, AP003990
- AlphaFoldDB: Q6ZHS4
- EnsemblPlants: Os02t0187800-01
- Gramene: Os02t0187800-01
- KEGG: dosa:Os02g0187800
- Orthologous matrix: FARNEHK
- InterPro: IPR002328, IPR011032, IPR013149
- PANTHER: PTHR42683
- SUPFAM: SSF50129, SSF51735
- PROSITE: PS00059
- Gene3D: 3.40.50.720, 3.90.180.10
- OrthoDB: Q6ZHS4
- SWISS-MODEL: Q6ZHS4
- Conserved Domain Database: cd05283
- eggNOG: KOG0023
Sequences
cDNA Sequence
- >LOC_Os02g09490.1
CTTTAATTATATATACTCGCTGCGGGACGCACCACCCCCTCTCCAGAATTCAGCGAGAGAGAGCGAATCAGCCACTGCACTTCTTGTTCTTGTTCTCTTCCTCCTCCCGAATCCGATCCATCCATTGGCCGCCGCCGCCGCCGCCGCCTCGTGCGTCGCGTGTTCGTGTCCAGAGACGATGGGCAGCCTCGCCGCCGAGAAGACCGTCACCGGGTGGGCCGCCAGGGACGCCTCCGGCCACCTCACCCCCTACAACTACACCCTCAGGAAGACTGGGCCTGAAGATGTGGTGGTGAAGGTTTTGTATTGTGGTATCTGCCATACTGACATCCACCAGGCCAAGAACCACCTTGGTGCTTCCAAGTACCCCATGGTCCCTGGCCATGAGGTGGTCGGCGAGGTGGTGGAGGTCGGGCCGGAGGTGACCAAGTACAGCGCCGGCGACGTCGTCGGCGTCGGCGTCATCGTCGGCTGCTGCCGCGAGTGCCATCCGTGCAAGGCCAATGTGGAGCAGTACTGCAACAAGAGGATTTGGTCCTACAACGACGTCTACACCGACGGCCGGCCAACCCAGGGCGGCTTCGCCTCCGCCATGGTCGTCGACCAGAAGTTTGTGGTGAAGATCCCGGCGGGGCTAGCGCCGGAGCAGGCGGCGCCGCTGCTGTGCGCCGGGCTGACGGTGTACAGCCCACTGAAGCACTTCGGGCTGATGTCGCCAGGTCTCCGCGGCGGCGTCCTGGGGCTCGGCGGCGTGGGGCACATGGGCGTGAAGGTGGCCAAGTCGATGGGGCACCACGTGACGGTGATCAGCTCGTCGGCGAGGAAGCGCGGCGAGGCCATGGACGACCTGGGCGCCGACGCCTACCTCGTCAGCTCCGACGCGGCGGCGATGGCGGCCGCCGGCGACTCGCTGGACTACATCATCGACACCGTGCCGGTGCACCACCCGCTGGAGCCGTACCTGGCGCTGCTGAAGCTGGACGGGAAGCTGATCCTGATGGGGGTGATCAACCAGCCGCTGAGCTTCATCTCGCCCATGGTGATGCTCGGCCGGAAGGCCATCACCGGCAGCTTCATCGGGAGCATGGCCGAGACGGAGGAGGTGCTCAACTTCTGCGTCGACAAGGGGCTCACCTCCCAGATCGAGGTCGTCAAGATGGACTACGTCAACCAGGCCCTCGAGCGCCTCGAGCGCAACGACGTCCGCTACCGCTTCGTCGTCGACGTCGCCGGCAGCAACATCGACGACGCCGACGCGCCGCCCGCCTGATCGCCGCCGTGCACCCTCATGGCAGCATCCGTATGATCCGTTCTTGAACTTGTTTGTGTGAGACTCTGACGACTTGTCCATGTAACGTCCGTGTGCCTTTCCGGTATAAATTCGGCCTCGCAATATATGGACCATTTTGCATTTCCGATCAAAATCATGCTTGCGTTTCACTTGCA
CDS Sequence
- >LOC_Os02g09490.1
ATGGGCAGCCTCGCCGCCGAGAAGACCGTCACCGGGTGGGCCGCCAGGGACGCCTCCGGCCACCTCACCCCCTACAACTACACCCTCAGGAAGACTGGGCCTGAAGATGTGGTGGTGAAGGTTTTGTATTGTGGTATCTGCCATACTGACATCCACCAGGCCAAGAACCACCTTGGTGCTTCCAAGTACCCCATGGTCCCTGGCCATGAGGTGGTCGGCGAGGTGGTGGAGGTCGGGCCGGAGGTGACCAAGTACAGCGCCGGCGACGTCGTCGGCGTCGGCGTCATCGTCGGCTGCTGCCGCGAGTGCCATCCGTGCAAGGCCAATGTGGAGCAGTACTGCAACAAGAGGATTTGGTCCTACAACGACGTCTACACCGACGGCCGGCCAACCCAGGGCGGCTTCGCCTCCGCCATGGTCGTCGACCAGAAGTTTGTGGTGAAGATCCCGGCGGGGCTAGCGCCGGAGCAGGCGGCGCCGCTGCTGTGCGCCGGGCTGACGGTGTACAGCCCACTGAAGCACTTCGGGCTGATGTCGCCAGGTCTCCGCGGCGGCGTCCTGGGGCTCGGCGGCGTGGGGCACATGGGCGTGAAGGTGGCCAAGTCGATGGGGCACCACGTGACGGTGATCAGCTCGTCGGCGAGGAAGCGCGGCGAGGCCATGGACGACCTGGGCGCCGACGCCTACCTCGTCAGCTCCGACGCGGCGGCGATGGCGGCCGCCGGCGACTCGCTGGACTACATCATCGACACCGTGCCGGTGCACCACCCGCTGGAGCCGTACCTGGCGCTGCTGAAGCTGGACGGGAAGCTGATCCTGATGGGGGTGATCAACCAGCCGCTGAGCTTCATCTCGCCCATGGTGATGCTCGGCCGGAAGGCCATCACCGGCAGCTTCATCGGGAGCATGGCCGAGACGGAGGAGGTGCTCAACTTCTGCGTCGACAAGGGGCTCACCTCCCAGATCGAGGTCGTCAAGATGGACTACGTCAACCAGGCCCTCGAGCGCCTCGAGCGCAACGACGTCCGCTACCGCTTCGTCGTCGACGTCGCCGGCAGCAACATCGACGACGCCGACGCGCCGCCCGCCTGA
Protein Sequence
- >LOC_Os02g09490.1
MGSLAAEKTVTGWAARDASGHLTPYNYTLRKTGPEDVVVKVLYCGICHTDIHQAKNHLGASKYPMVPGHEVVGEVVEVGPEVTKYSAGDVVGVGVIVGCCRECHPCKANVEQYCNKRIWSYNDVYTDGRPTQGGFASAMVVDQKFVVKIPAGLAPEQAAPLLCAGLTVYSPLKHFGLMSPGLRGGVLGLGGVGHMGVKVAKSMGHHVTVISSSARKRGEAMDDLGADAYLVSSDAAAMAAAGDSLDYIIDTVPVHHPLEPYLALLKLDGKLILMGVINQPLSFISPMVMLGRKAITGSFIGSMAETEEVLNFCVDKGLTSQIEVVKMDYVNQALERLERNDVRYRFVVDVAGSNIDDADAPPA*