Gene Details:

• MSU gene ID: LOC_Os02g08440
• RAPdb gene ID: Os02g0181300
• Gene Symbol: OsWRKY71
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• To address the cross-talk of GA and ABA signaling, we studied two rice WRKY genes (OsWRKY51 and OsWRKY71) that are ABA-inducible and GA-repressible in embryos and aleurone cells.
• However, two of these non-DNA-binding mutants are still able to repress GA induction by enhancing the binding affinity of the wild-type DNA-binding OsWRKY71 repressors.
• In contrast, the third non-DNA-binding mutant enhances GA induction of Amy32b-GUS, by interfering with the binding of the wild-type OsWRKY71 or the OsWRKY71/OsWRKY51 repressing complex.
• The expression analyses of the group IIa WRKY transcription factors in rice revealed that OsWRKY28, together with OsWRKY71, exhibit an early-induced expression prior to the late-induced expressions of OsWRKY62 and OsWRKY76.
• OsWRKY71, a rice transcription factor, is involved in rice defense response.
• One of them, OsWRKY71, was up-regulated by several defense signaling molecules, such as SA, methyl jasmonate (MeJA), 1-aminocyclo-propane-1-carboxylic acid (ACC), as well as wounding and pathogen infection, suggesting that OsWRKY71 might function in rice biotic stress response.
• Furthermore, two marker genes in defense signaling pathway, OsNPR1 and OsPR1b, were constitutively expressed in OsWRKY71-overexpressing transgenic plants.
• These results suggest that OsWRKY71 might function as a transcriptional regulator upstream of OsNPR1 and OsPR1b in rice defense signaling pathways.
• Mutation of the two W boxes prevents the binding of OsWRKY71 to the mutated promoter, and releases the suppression of the OsGAMYB-activated Amy32b expression by OsWRKY71, suggesting that OsWRKY71 blocks GA signaling by functionally interfering with OsGAMYB.
• Exogenous GA treatment decreases the steady-state mRNA level of OsWRKY71 and destabilizes the GFP:OsWRKY71 fusion protein.
• These findings suggest that OsWRKY71 encodes a transcriptional repressor of GA signaling in aleurone cells.
• Third, unlike OsWRKY71, OsWRKY24 is stable upon GA treatment.
• Microarray analysis revealed that several elicitor-induced defense-related genes were upregulated in rice cells overexpressing OsWRKY71.
• These results indicate that the activation of defense-related genes by OsWRKY71 was probably indirect.

Function-related keywords:

• ga ,  transcription-factor ,  defense-response ,  defense ,  jasmonate ,  ABA ,  biotic-stress

Literature:

• A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells .  DOI:  10.1007/s11103-009-9463-4 ;  PMID:  19199048
• OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus .  DOI:  10.1007/s11103-013-0032-5 ;  PMID:  23462973
• Characterization of an elicitor-induced rice WRKY gene, OsWRKY71 .  DOI:  10.1271/bbb.70553 ;  PMID:  18175928
• OsWRKY71, a rice transcription factor, is involved in rice defense response .  DOI:  10.1016/j.jplph.2006.07.006 ;  PMID:  16919842
• Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells .  DOI:  10.1111/j.1365-313X.2006.02694.x ;  PMID:  16623886
• The WRKY Gene Family in Rice (Oryza sativa) .  DOI:  10.1111/j.1744-7909.2007.00504.x
• A rice WRKY gene encodes a transcriptional repressor of the gibberellin signaling pathway in aleurone cells .  DOI:  10.1104/pp.103.034967 ;  PMID:  15047897
• Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells .  DOI:  10.1104/pp.104.054312 ;  PMID:  15618416
• IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways .  DOI:  10.3390/ijms22126605 ;  PMID:  34203082

Related News:

Gene Resources:

• NCBI ID: AB190817  , BK005074
• UniProt accessions:

Sequences: