Gene Details:

• MSU gene ID: LOC_Os02g07430
• RAPdb gene ID: Os02g0170300
• Gene Symbol: OsMADS29
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Ectopic expression of MADS29 resulted in a severely dwarfed phenotype, exhibiting elevated levels of cytokinin, thereby suggesting that cytokinin biosynthesis pathway could be one of the major targets of OsMADS29.
• Our analyses show that OsMADS29 is expressed in female reproductive organs including the ovule, ovule vasculature, and the whole seed except for the outer layer cells of the pericarp.
• Overexpression of OsMADS29 in heterologous BY2 cells was found to mimic the effects of exogenous application of cytokinins that causes differentiation of proplastids to starch-containing amyloplasts and activation of genes involved in the starch biosynthesis pathway.
• Our results suggest that OsMADS29 has important functions in seed development of rice by regulating cell degeneration of maternal tissues.
• Live and let die - the B(sister) MADS-box gene OsMADS29 controls the degeneration of cells in maternal tissues during seed development of rice (Oryza sativa).
• Knock-down of OsMADS29 by double-stranded RNA-mediated interference (RNAi) results in shriveled and/or aborted seeds.
• However, analyses involving OsMADS29 protein expression domains and characterization of OsMADS29 gain-of-function and knockdown phenotypes revealed novel aspects of its function in maintaining hormone homeostasis, which may have a role in the development of embryo and plastid differentiation and starch filling in endosperm cells.
• OsMADS29 is a seed-specific MADS-box transcription factor that affects embryo development and grain filling by maintaining hormone homeostasis and degradation of cells in the nucellus and nucellar projection.
• Although it has a bipartite nuclear localization signal (NLS) sequence, the transiently expressed OsMADS29 monomer does not localize specifically in the nucleus.
• Dimerization of the monomers alters the intracellular localization fate of the resulting OsMADS29 homodimer, which then translocates into the nucleus.
• OsMADS29 (M29) is a seed-specific MADS-box transcription factor involved in programmed cell death of nucellar tissue and maintaining auxin:cytokinin homeostasis.
• OsMADS29 (M29) is a crucial regulator of seed development in rice.

Function-related keywords:

• dwarf ,  pericarp ,  cytokinin ,  seed-development ,  seed ,  reproductive ,  endosperm ,  starch-biosynthesis ,  homeostasis ,  starch ,  grain ,  development ,  nucleus ,  transcription-factor ,  cell-death ,  programmed-cell-death

Literature:

• Functional delineation of rice MADS29 reveals its role in embryo and endosperm development by affecting hormone homeostasis .  DOI:  10.1093/jxb/ert231 ;  PMID:  23929654
• The MADS29 transcription factor regulates the degradation of the nucellus and the nucellar projection during rice seed development .  DOI:  10.1105/tpc.111.094854 ;  PMID:  22408076
• Live and let die - the B(sister) MADS-box gene OsMADS29 controls the degeneration of cells in maternal tissues during seed development of rice (Oryza sativa) .  DOI:  10.1371/journal.pone.0051435 ;  PMID:  23251532
• Post-translational regulation of rice MADS29 function: homodimerization or binary interactions with other seed-expressed MADS proteins modulate its translocation into the nucleus .  DOI:  10.1093/jxb/eru296 ;  PMID:  25096923
• Characterization of Transcription Regulatory Domains of OsMADS29: Identification of Proximal Auxin-Responsive Domains and a Strong Distal Negative Element .  DOI:  10.3389/fpls.2022.850956 ;  PMID:  35557721
• Ca2+ -Calmodulin regulates nuclear translocation of the rice seed-specific MADS-box transcription factor OsMADS29 .  DOI:  10.1111/febs.16767 ;  PMID:  36861329

Related News:

Gene Resources:

• NCBI ID: AK109522
• UniProt accessions:

Sequences: