Information report for OsMADS29
Gene Details
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Functional Descriptions
- Ectopic expression of MADS29 resulted in a severely dwarfed phenotype, exhibiting elevated levels of cytokinin, thereby suggesting that cytokinin biosynthesis pathway could be one of the major targets of OsMADS29.
- Our analyses show that OsMADS29 is expressed in female reproductive organs including the ovule, ovule vasculature, and the whole seed except for the outer layer cells of the pericarp.
- Overexpression of OsMADS29 in heterologous BY2 cells was found to mimic the effects of exogenous application of cytokinins that causes differentiation of proplastids to starch-containing amyloplasts and activation of genes involved in the starch biosynthesis pathway.
- Our results suggest that OsMADS29 has important functions in seed development of rice by regulating cell degeneration of maternal tissues.
- Live and let die - the B(sister) MADS-box gene OsMADS29 controls the degeneration of cells in maternal tissues during seed development of rice (Oryza sativa).
- Knock-down of OsMADS29 by double-stranded RNA-mediated interference (RNAi) results in shriveled and/or aborted seeds.
- However, analyses involving OsMADS29 protein expression domains and characterization of OsMADS29 gain-of-function and knockdown phenotypes revealed novel aspects of its function in maintaining hormone homeostasis, which may have a role in the development of embryo and plastid differentiation and starch filling in endosperm cells.
- OsMADS29 is a seed-specific MADS-box transcription factor that affects embryo development and grain filling by maintaining hormone homeostasis and degradation of cells in the nucellus and nucellar projection.
- Although it has a bipartite nuclear localization signal (NLS) sequence, the transiently expressed OsMADS29 monomer does not localize specifically in the nucleus.
- Dimerization of the monomers alters the intracellular localization fate of the resulting OsMADS29 homodimer, which then translocates into the nucleus.
- OsMADS29 (M29) is a seed-specific MADS-box transcription factor involved in programmed cell death of nucellar tissue and maintaining auxin:cytokinin homeostasis.
- OsMADS29 (M29) is a crucial regulator of seed development in rice.
Functional Keywords
- dwarf , pericarp , cytokinin , seed-development , seed , reproductive , endosperm , starch-biosynthesis , homeostasis , starch , grain , development , nucleus , transcription-factor , cell-death , programmed-cell-death
Literature and News
- Functional delineation of rice MADS29 reveals its role in embryo and endosperm development by affecting hormone homeostasis . DOI: 10.1093/jxb/ert231 ; PMID: 23929654
- The MADS29 transcription factor regulates the degradation of the nucellus and the nucellar projection during rice seed development . DOI: 10.1105/tpc.111.094854 ; PMID: 22408076
- Live and let die - the B(sister) MADS-box gene OsMADS29 controls the degeneration of cells in maternal tissues during seed development of rice (Oryza sativa) . DOI: 10.1371/journal.pone.0051435 ; PMID: 23251532
- Post-translational regulation of rice MADS29 function: homodimerization or binary interactions with other seed-expressed MADS proteins modulate its translocation into the nucleus . DOI: 10.1093/jxb/eru296 ; PMID: 25096923
- Characterization of Transcription Regulatory Domains of OsMADS29: Identification of Proximal Auxin-Responsive Domains and a Strong Distal Negative Element . DOI: 10.3389/fpls.2022.850956 ; PMID: 35557721
- Ca2+ -Calmodulin regulates nuclear translocation of the rice seed-specific MADS-box transcription factor OsMADS29 . DOI: 10.1111/febs.16767 ; PMID: 36861329
Gene Resources
- UniProt: Q6H711
- EMBL: AK109522, AP004113, AP004836
- AlphaFoldDB: Q6H711
- EnsemblPlants: Os02t0170300-01
- Gramene: Os02t0170300-01
- KEGG: dosa:Os02g0170300
- Orthologous matrix: FTHSTYY
- InterPro: IPR002100, IPR002487, IPR033896
Sequences
cDNA Sequence
- >LOC_Os02g07430.1
AGCCTTTGAAGCAAGCTTAGCACTTCACAAGTTTCCTCTTGTGCAGAAGAAGGAGAAACAAAAGAACACAGAAATTAAATTAAACTCGATCAGGAAGGTATAGCAGGCATGGGGCGCGGCAAGATCGAGATCAAGAGGATCGAGAACGCGACGAACAGGCAGGTGACATTCTCGAAGAGGCGGGGAGGGCTACTGAAGAAGGCGAACGAGCTCGCCGTGCTCTGCGACGCCCGCGTCGGCGTCGTCATCTTCTCCAGCACCGGCAAGATGTTCGAGTACTGCAGCCCTACCTGCAGTTTGAGGGAACTCATCGAGCATTACCAGACCGTCACCAACACTCATTTCGAGGAGATCAACCACGATCAGCAAATATTTGTGGAGATGACTCGGATGAGGAACGAGATGGAGAAGCTGGACGGTGGCATCAGGAGGTTCACCGGCGACGACCTCTCCAACCTCACGCTCGCCGACATCAACGATCTCGAGCAGCAGCTCGAATTCTCCGTCACCAAAGTCCGTGCGAGAAAGCATCAGCTCCTGAACCAGCAGCTGGACAACCTTCGTCGCAAGGAGCACATCCTGGAAGACCAGAACAGCTTCCTGTGCCGCATGATCAACGAGAACCATCATCAGGCGGCGGTGGGCGGCGGCGACGTGAAGGCGATGGTGGAGATGGCGCCGGTGCTGTCGATGCTGACGGCGGCGCCGGCGTACTACGGCGAGGAGTCGTCGAGCACCGCGCTGCAGCTCACCCCGCCGCTGCACGCCGTCGACGCCGCCGCCGCCGCCGGGTTCCGGCTGCAGCCGACGCAGCCCAACCTGCAGGACCCCGGCTGCAGCAGCAGCAGCTTCCATGCCGCCGCCGCCGGCCACGGCCTGCAGCTGTGGTAAGAACAAACGAAGCAAAGCATACATGGCACATCCTGATACTCTCATCAGAGATCTAGATGATTGCTTGCTAATTAAGCACATGATGGCATTGTCGATTCTGGGAGCTAGGAGTAACTTGGAGACCATGAAGATGCATGATCAGCTGATGATAGACGATCGAGTTGGGTTGCTGTGTTGTTCAATTGTTCATCGTTCTGGACATATATATATATATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTAATTAGCACATCAGAATAATCAAGCTTTTCTACCTAGTTAACTAGTACAGCTGCTAAGTACCTTTTGATTTGTTGGTGTGTGAACTACTGAACTGGAGTTGGATATTAAGGTCTCCATGCATTTGCATGGTTAATTAGCAGAGAGATCATTGAGGTTTTTAGGATGTAACA
CDS Sequence
- >LOC_Os02g07430.1
ATGGGGCGCGGCAAGATCGAGATCAAGAGGATCGAGAACGCGACGAACAGGCAGGTGACATTCTCGAAGAGGCGGGGAGGGCTACTGAAGAAGGCGAACGAGCTCGCCGTGCTCTGCGACGCCCGCGTCGGCGTCGTCATCTTCTCCAGCACCGGCAAGATGTTCGAGTACTGCAGCCCTACCTGCAGTTTGAGGGAACTCATCGAGCATTACCAGACCGTCACCAACACTCATTTCGAGGAGATCAACCACGATCAGCAAATATTTGTGGAGATGACTCGGATGAGGAACGAGATGGAGAAGCTGGACGGTGGCATCAGGAGGTTCACCGGCGACGACCTCTCCAACCTCACGCTCGCCGACATCAACGATCTCGAGCAGCAGCTCGAATTCTCCGTCACCAAAGTCCGTGCGAGAAAGCATCAGCTCCTGAACCAGCAGCTGGACAACCTTCGTCGCAAGGAGCACATCCTGGAAGACCAGAACAGCTTCCTGTGCCGCATGATCAACGAGAACCATCATCAGGCGGCGGTGGGCGGCGGCGACGTGAAGGCGATGGTGGAGATGGCGCCGGTGCTGTCGATGCTGACGGCGGCGCCGGCGTACTACGGCGAGGAGTCGTCGAGCACCGCGCTGCAGCTCACCCCGCCGCTGCACGCCGTCGACGCCGCCGCCGCCGCCGGGTTCCGGCTGCAGCCGACGCAGCCCAACCTGCAGGACCCCGGCTGCAGCAGCAGCAGCTTCCATGCCGCCGCCGCCGGCCACGGCCTGCAGCTGTGGTAA
Protein Sequence
- >LOC_Os02g07430.1
MGRGKIEIKRIENATNRQVTFSKRRGGLLKKANELAVLCDARVGVVIFSSTGKMFEYCSPTCSLRELIEHYQTVTNTHFEEINHDQQIFVEMTRMRNEMEKLDGGIRRFTGDDLSNLTLADINDLEQQLEFSVTKVRARKHQLLNQQLDNLRRKEHILEDQNSFLCRMINENHHQAAVGGGDVKAMVEMAPVLSMLTAAPAYYGEESSSTALQLTPPLHAVDAAAAAGFRLQPTQPNLQDPGCSSSSFHAAAAGHGLQLW*