Gene Details:

Functional Descriptions:

  • Herein, we report a transporter, Nrat1 (Nramp aluminum transporter 1), specific for trivalent Al ion in rice.
  • We therefore concluded that Nrat1 is a plasma membrane-localized transporter for trivalent Al, which is required for a prior step of final Al detoxification through sequestration of Al into vacuoles.
  • Nrat1 is localized at the plasma membranes of all cells of root tips except epidermal cells.
  • Expression of Nrat1 is up-regulated by Al in the roots and regulated by a C2H2 zinc finger transcription factor (ART1).
  • When expressed in yeast, Nrat1 transports trivalent Al ion, but not other divalent ions, such as manganese, iron, and cadmium, or the Al-citrate complex.
  • Knockout of Nrat1 resulted in decreased Al uptake, increased Al binding to cell wall, and enhanced Al sensitivity, but did not affect the tolerance to other metals.
  • Genome-wide association mapping of aluminum toxicity tolerance and fine mapping of a candidate gene for Nrat1 in rice.
  • Haplotype analysis of Nrat1 using 327 3K RGP accessions indicated that minor allele variations in aus and indica subpopulations decreased Al toxicity tolerance in rice.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os02g03900.1
    GAGCAAGACATACTTTTTCATATCTTCCAGACAAGGTGCATTAGCAACATAGAATATGGAAGGGACTGGTGAGATGAGAGAGGTTGGACGAGAAACTCTCCATGGAGGTGTGGTGCAGAGTGTTAGTGAGACTGATGAGTACAAAGAAAAGACTATTGACAGTGAAAAAGATGGGCAGTTTCGGGTGCAGCCAAGATGGAGGAAATTCTTGGCTCATGTTGGGCCTGGAGCTCTGGTGGCCATTGGCTTCCTAGATCCTAGCAACTTGGAAACTGACATGCAAGCTGGAGCTGACTTCAAGTATGAGCTTCTGTGGGTCATCTTAGTTGGGATGGTCTTTGCACTTCTGATCCAAACATTAGCTGCCAACCTTGGAGTGAAAACAGGAAGGCACCTTGCTGAACTGTGCAGGGAAGAGTACCCGCACTATGTCAACATTTTCTTATGGATCATTGCGGAGTTGGCGGTGATTTCTGATGACATTCCTGAAGTGTTGGGCACAGCTTTTGCGTTCAACATATTGCTTAAGATTCCAGTGTGGGCTGGAGTTATTCTCACAGTGTTCAGCACCCTCTTGCTTCTTGGAGTACAGAGATTTGGGGCTCGGAAGCTGGAGTTCATCATAGCAGCATTCATGTTCACTATGGCAGCTTGCTTCTTCGGAGAGCTAAGCTATTTGAGGCCGTCTGCAGGAGAGGTGGTCAAGGGCATGTTTGTCCCTTCTCTCCAAGGGAAAGGCGCTGCTGCCAATGCGATCGCACTCTTTGGCGCTATCATCACACCATACAACTTGTTCTTGCACTCTGCTCTGGTCCTGTCAAGGAAGACCCCACGATCAGACAAAAGCATCAGAGCTGCTTGCAGATACTTCCTTATTGAGTGTAGTCTTGCATTCATCGTGGCTTTCCTCATAAATGTTTCAGTCGTTGTTGTTGCTGGATCCATTTGCAATGCGAATAATCTTTCTCCAGCTGATGCCAACACGTGTGGTGACCTAACTCTGCAATCCACACCTCTTTTACTCAGGAATGTTTTGGGGAGATCAAGCTCGGTGGTGTATGCTGTTGCGCTGCTAGCTTCTGGGCAAAGCACCACTATCAGTTGCACGTTCGCTGGGCAGGTCATCATGCAGGGGTTTCTGGACATGAAGATGAAGAATTGGGTAAGGAACCTCATCACCCGAGTCATCGCCATAGCTCCTAGCCTCATCGTCTCCATTGTCAGTGGTCCCTCAGGCGCCGGCAAGCTAATCATCCTGTCATCAATGATCCTGTCATTTGAGCTGCCGTTCGCTCTCATCCCTCTACTCAAGTTCTGCAACAGCAGCAAGAAAGTTGGACCCCTCAAGGAATCCATCTATACGGTGGTGATCGCGTGGATCCTGAGCTTCGCGCTCATCGTCGTCAACACCTACTTCCTAGTGTGGACATACGTGGACTGGCTCGTCCACAACAACCTCCCCAAGTATGCCAATGGCCTCATCTCCGTGGTGGTGTTCGCGCTCATGGCCGCCTACCTGGTCGCCGTCGTCTACCTGACGTTCAGGAAGGACACGGTGGCGACGTACGTGCCGGTGCCGGAGCGGGCGCAGGCGCAGGTGGAGGCCGGCGGGACGCCGGTGGTGGACGCCTCCGCCGCCGACGAGGACCAGCCGGCTCCGTACAGGAAGGACCTTGCCGATGCTTCCATGTAGCCTTGATAAATTATTGCTTGCTGAGTAGTGCATTAGTTATTCCTCGTCGTCAGTGTATAACTAGATGTGTTCTCTGGATGATATAATGTGATTCGTCATTGATTTTTTGTTCTTCTTCTTGTTGTTGTTTCTAAGCAGCTTAGCTGTAAATTAAGTTTGAGAAAAAAAAGTGTGCACTACT
CDS Sequence
  • >LOC_Os02g03900.1
    ATGGAAGGGACTGGTGAGATGAGAGAGGTTGGACGAGAAACTCTCCATGGAGGTGTGGTGCAGAGTGTTAGTGAGACTGATGAGTACAAAGAAAAGACTATTGACAGTGAAAAAGATGGGCAGTTTCGGGTGCAGCCAAGATGGAGGAAATTCTTGGCTCATGTTGGGCCTGGAGCTCTGGTGGCCATTGGCTTCCTAGATCCTAGCAACTTGGAAACTGACATGCAAGCTGGAGCTGACTTCAAGTATGAGCTTCTGTGGGTCATCTTAGTTGGGATGGTCTTTGCACTTCTGATCCAAACATTAGCTGCCAACCTTGGAGTGAAAACAGGAAGGCACCTTGCTGAACTGTGCAGGGAAGAGTACCCGCACTATGTCAACATTTTCTTATGGATCATTGCGGAGTTGGCGGTGATTTCTGATGACATTCCTGAAGTGTTGGGCACAGCTTTTGCGTTCAACATATTGCTTAAGATTCCAGTGTGGGCTGGAGTTATTCTCACAGTGTTCAGCACCCTCTTGCTTCTTGGAGTACAGAGATTTGGGGCTCGGAAGCTGGAGTTCATCATAGCAGCATTCATGTTCACTATGGCAGCTTGCTTCTTCGGAGAGCTAAGCTATTTGAGGCCGTCTGCAGGAGAGGTGGTCAAGGGCATGTTTGTCCCTTCTCTCCAAGGGAAAGGCGCTGCTGCCAATGCGATCGCACTCTTTGGCGCTATCATCACACCATACAACTTGTTCTTGCACTCTGCTCTGGTCCTGTCAAGGAAGACCCCACGATCAGACAAAAGCATCAGAGCTGCTTGCAGATACTTCCTTATTGAGTGTAGTCTTGCATTCATCGTGGCTTTCCTCATAAATGTTTCAGTCGTTGTTGTTGCTGGATCCATTTGCAATGCGAATAATCTTTCTCCAGCTGATGCCAACACGTGTGGTGACCTAACTCTGCAATCCACACCTCTTTTACTCAGGAATGTTTTGGGGAGATCAAGCTCGGTGGTGTATGCTGTTGCGCTGCTAGCTTCTGGGCAAAGCACCACTATCAGTTGCACGTTCGCTGGGCAGGTCATCATGCAGGGGTTTCTGGACATGAAGATGAAGAATTGGGTAAGGAACCTCATCACCCGAGTCATCGCCATAGCTCCTAGCCTCATCGTCTCCATTGTCAGTGGTCCCTCAGGCGCCGGCAAGCTAATCATCCTGTCATCAATGATCCTGTCATTTGAGCTGCCGTTCGCTCTCATCCCTCTACTCAAGTTCTGCAACAGCAGCAAGAAAGTTGGACCCCTCAAGGAATCCATCTATACGGTGGTGATCGCGTGGATCCTGAGCTTCGCGCTCATCGTCGTCAACACCTACTTCCTAGTGTGGACATACGTGGACTGGCTCGTCCACAACAACCTCCCCAAGTATGCCAATGGCCTCATCTCCGTGGTGGTGTTCGCGCTCATGGCCGCCTACCTGGTCGCCGTCGTCTACCTGACGTTCAGGAAGGACACGGTGGCGACGTACGTGCCGGTGCCGGAGCGGGCGCAGGCGCAGGTGGAGGCCGGCGGGACGCCGGTGGTGGACGCCTCCGCCGCCGACGAGGACCAGCCGGCTCCGTACAGGAAGGACCTTGCCGATGCTTCCATGTAG
Protein Sequence
  • >LOC_Os02g03900.1
    MEGTGEMREVGRETLHGGVVQSVSETDEYKEKTIDSEKDGQFRVQPRWRKFLAHVGPGALVAIGFLDPSNLETDMQAGADFKYELLWVILVGMVFALLIQTLAANLGVKTGRHLAELCREEYPHYVNIFLWIIAELAVISDDIPEVLGTAFAFNILLKIPVWAGVILTVFSTLLLLGVQRFGARKLEFIIAAFMFTMAACFFGELSYLRPSAGEVVKGMFVPSLQGKGAAANAIALFGAIITPYNLFLHSALVLSRKTPRSDKSIRAACRYFLIECSLAFIVAFLINVSVVVVAGSICNANNLSPADANTCGDLTLQSTPLLLRNVLGRSSSVVYAVALLASGQSTTISCTFAGQVIMQGFLDMKMKNWVRNLITRVIAIAPSLIVSIVSGPSGAGKLIILSSMILSFELPFALIPLLKFCNSSKKVGPLKESIYTVVIAWILSFALIVVNTYFLVWTYVDWLVHNNLPKYANGLISVVVFALMAAYLVAVVYLTFRKDTVATYVPVPERAQAQVEAGGTPVVDASAADEDQPAPYRKDLADASM*