Gene Details:

• MSU gene ID: LOC_Os02g03410
• RAPdb gene ID: Os02g0126400
• Gene Symbol: OsCPK4
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• A significant number of genes involved in lipid metabolism and protection against oxidative stress appear to be up-regulated by OsCPK4 in roots of overexpressor plants.
• Taken together, our data show that OsCPK4 functions as a positive regulator of the salt and drought stress responses in rice via the protection of cellular membranes from stress-induced oxidative damage.
• Meanwhile, OsCPK4 overexpression has no effect on the expression of well-characterized abiotic stress-associated transcriptional regulatory networks (i.
• Overexpression of OsCPK4 in rice plants significantly enhances tolerance to salt and drought stress.
• Compared with control plants, OsCPK4 overexpressor plants exhibit stronger water-holding capability and reduced levels of membrane lipid peroxidation and electrolyte leakage under drought or salt stress conditions.
• Moreover, a plasma membrane localization of OsCPK4 was observed by transient expression assays of green fluorescent protein-tagged OsCPK4 in onion (Allium cepa) epidermal cells.
• Here, we report that OsCPK4 expression is induced by high salinity, drought, and the phytohormone abscisic acid.
• Also, salt-treated OsCPK4 seedlings accumulate less Na(+) in their roots.
• Enhancing blast disease resistance by overexpression of the calcium-dependent protein kinase OsCPK4 in rice.
• It shows that overexpression of OsCPK4 gene in rice plants enhances resistance to blast disease by preventing fungal penetration.
• Altogether, our findings indicate that OsCPK4 is a potential molecular target to improve not only abiotic stress tolerance, but also blast disease resistance of rice crops.
• Given that OsCPK4 overexpression was known to confer also salt and drought tolerance in rice, the results reported in this article demonstrate that OsCPK4 acts as a convergence component that positively modulates both biotic and abiotic signalling pathways.
• OsCPK4 overexpression leads also to constitutive increased content of the glycosylated salicylic acid hormone in leaves without compromising rice yield.
• In this work, the isoform OsCPK4 of the rice calcium-dependent protein kinase family is reported as a regulator of rice immunity to blast fungal infection.
• The constitutive accumulation of OsCPK4 protein prepares rice plants for a rapid and potentiated defence response, including the production of reactive oxygen species, callose deposition and defence gene expression.
• The calcium-dependent protein kinase OsCPK4 has been demonstrated to play important roles in salt and drought tolerance, plant growth and development in rice.
• In this study, we demonstrated that the generation of oxidative burst and pathogenesis-related gene expression triggered by microbe-associated molecular patterns (MAMPs) were significantly enhanced in the OsCPK4 mutants.
• These mutant lines are more resistant to bacterial blight and fungal blast diseases than the wild-type plants, indicating that OsCPK4 negatively regulates innate immunity in rice.
• However, little is known on molecular mechanisms underlying OsCPK4 function in rice immunity.
• These findings indicate that the kinase-inactive OsCPK4 promotes OsRLCK176 degradation and restricts plant defenses; whereas the activation of OsCPK4-OsRLCK176 phosphorylation circuit invalidates the OsRLCK176 degradation machinery and thus enhancing plant immunity.
• The kinase OsCPK4 regulates a buffering mechanism that fine-tunes innate immunity.
• OsCPK4 was further identified to interact with a receptor-like cytoplasmic kinase OsRLCK176.
• Moreover, the kinase activity and phosphorylation of OsCPK4 and OsRLCK176 contribute to the stability of OsRLCK176.

Function-related keywords:

• oxidative-stress ,  oxidative ,  abiotic-stress ,  drought ,  salt ,  salt-stress ,  tolerance ,  stress ,  plasma-membrane ,  phytohormone ,  drought-stress ,  abscisic-acid ,  drought-stress-response ,  stress-response ,  biotic-stress ,  seedlings ,  resistance ,  yield ,  drought-tolerance ,  disease ,  salicylic-acid ,  blast ,  disease-resistance ,  immunity ,  blast-disease ,  stress-tolerance ,  Kinase ,  reactive-oxygen-species ,  defence ,  protein-kinase ,  defence-response ,  growth ,  development ,  innate-immunity ,  blight ,  resistant ,  bacterial-blight ,  plant-growth ,  fungal-blast

Literature:

• Overexpression of a Calcium-Dependent Protein Kinase Confers Salt and Drought Tolerance in Rice by Preventing Membrane Lipid Peroxidation .  DOI:  10.1104/pp.113.230268 ;  PMID:  24784760
• Enhancing blast disease resistance by overexpression of the calcium-dependent protein kinase OsCPK4 in rice .  DOI:  10.1111/pbi.12500 ;  PMID:  26578239
• The Kinase OsCPK4 Regulates a Buffering Mechanism That Fine-Tunes Innate Immunity .  DOI:  10.1104/pp.17.01024 ;  PMID:  29242377
• Conserved Degradation of Orthologous RLCKs Regulates Immune Homeostasis .  DOI:  10.1016/j.tplants.2018.05.001 ;  PMID:  29776748

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: