OsCPK4

Gene Details

MSU gene ID: LOC_Os02g03410
RAPdb gene ID: Os02g0126400
Gene Symbol: OsCPK4
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

A significant number of genes involved in lipid metabolism and protection against oxidative stress appear to be up-regulated by OsCPK4 in roots of overexpressor plants.
Taken together, our data show that OsCPK4 functions as a positive regulator of the salt and drought stress responses in rice via the protection of cellular membranes from stress-induced oxidative damage.
Meanwhile, OsCPK4 overexpression has no effect on the expression of well-characterized abiotic stress-associated transcriptional regulatory networks (i.
Overexpression of OsCPK4 in rice plants significantly enhances tolerance to salt and drought stress.
Compared with control plants, OsCPK4 overexpressor plants exhibit stronger water-holding capability and reduced levels of membrane lipid peroxidation and electrolyte leakage under drought or salt stress conditions.
Moreover, a plasma membrane localization of OsCPK4 was observed by transient expression assays of green fluorescent protein-tagged OsCPK4 in onion (Allium cepa) epidermal cells.
Here, we report that OsCPK4 expression is induced by high salinity, drought, and the phytohormone abscisic acid.
Also, salt-treated OsCPK4 seedlings accumulate less Na(+) in their roots.
Enhancing blast disease resistance by overexpression of the calcium-dependent protein kinase OsCPK4 in rice.
It shows that overexpression of OsCPK4 gene in rice plants enhances resistance to blast disease by preventing fungal penetration.
Altogether, our findings indicate that OsCPK4 is a potential molecular target to improve not only abiotic stress tolerance, but also blast disease resistance of rice crops.
Given that OsCPK4 overexpression was known to confer also salt and drought tolerance in rice, the results reported in this article demonstrate that OsCPK4 acts as a convergence component that positively modulates both biotic and abiotic signalling pathways.
OsCPK4 overexpression leads also to constitutive increased content of the glycosylated salicylic acid hormone in leaves without compromising rice yield.
In this work, the isoform OsCPK4 of the rice calcium-dependent protein kinase family is reported as a regulator of rice immunity to blast fungal infection.
The constitutive accumulation of OsCPK4 protein prepares rice plants for a rapid and potentiated defence response, including the production of reactive oxygen species, callose deposition and defence gene expression.
The calcium-dependent protein kinase OsCPK4 has been demonstrated to play important roles in salt and drought tolerance, plant growth and development in rice.
In this study, we demonstrated that the generation of oxidative burst and pathogenesis-related gene expression triggered by microbe-associated molecular patterns (MAMPs) were significantly enhanced in the OsCPK4 mutants.
These mutant lines are more resistant to bacterial blight and fungal blast diseases than the wild-type plants, indicating that OsCPK4 negatively regulates innate immunity in rice.
However, little is known on molecular mechanisms underlying OsCPK4 function in rice immunity.
These findings indicate that the kinase-inactive OsCPK4 promotes OsRLCK176 degradation and restricts plant defenses; whereas the activation of OsCPK4-OsRLCK176 phosphorylation circuit invalidates the OsRLCK176 degradation machinery and thus enhancing plant immunity.
The kinase OsCPK4 regulates a buffering mechanism that fine-tunes innate immunity.
OsCPK4 was further identified to interact with a receptor-like cytoplasmic kinase OsRLCK176.
Moreover, the kinase activity and phosphorylation of OsCPK4 and OsRLCK176 contribute to the stability of OsRLCK176.

Functional Keywords

Literature and News

Gene Resources

Homologs

Sequences

cDNA Sequence

>LOC_Os02g03410.1
ACCTTCCTCTCCACCTTCCTCTTCCTCCCGACCAAACAACCTCCCTCCTCCTCCTCCACCTCGTCGTCGTCTCCGCCGCGCTGTCTCCTCCTCTATATACCCTCACCCACACTCGCACGCGCAAACCCAACCCCCGCATATGCTTAACTCCCCAACCCAAACCCTCCTCCTCCCCCATGGGCGCGTGCTTCTCATCCCACACTGCGACCGCCGCCGCCGATGGCGGGAGCGGGAAGCGGCAGCAGCGGAAGGGGGATCACAAGGGGAAGCTCCCCGATGGCGGCGGCGGCGAGAAGGAGAAGGAGGCGGCGCGGGTGGAGTTCGGGTACGAGAGGGACTTCGAGGGGAGGTACCAGGTCGGGAGGCTGCTCGGCCACGGCCAGTTCGGCTACACCTTCGCCGCCACCGACCGGGCCTCCGGTGACCGCGTCGCCGTCAAGCGCATCGACAAGGCCAAGATGGTTCGCCCTGTTGCTGTGGAGGATGTAAAGAGAGAAGTGAAGATTCTTAAAGAACTTAAAGGCCATGAGAATATTGTTCACTTCTACAATGCGTTTGAAGATGACTCATATGTATATATTGTGATGGAACTATGTGAGGGTGGTGAACTATTGGACCGGATTTTGGCAAAAAAGAACAGCCGTTATAGTGAGAAAGATGCTGCAGTGGTGGTGCGGCAGATGCTCAAAGTGGCAGCTGAGTGCCATCTGCATGGGCTAGTTCACCGAGATATGAAGCCCGAGAACTTCCTTTTCAAATCAACCAAGGAGGACTCACCTTTAAAGGCAACAGATTTTGGTCTGTCAGACTTCATAAAACCAGGGAAAAAGTTTCACGATATAGTTGGCAGTGCCTATTATGTAGCACCAGAAGTTTTAAAACGACGGTCTGGCCCTGAGTCAGATGTTTGGAGCATAGGAGTCATAACTTATATTTTGCTCTGTGGGAGACGCCCTTTTTGGAATAAGACAGAGGATGGCATATTCAGAGAGGTACTAAGAAACAAGCCTGATTTTCGTAAGAAGCCTTGGCCAGGCATCAGTTCAGGTGCTAAAGATTTCGTTAAAAAGTTACTTGTAAAGAACCCAAGGGCAAGATTAACCGCTGCTCAAGCTCTCTCGCATCCATGGGTAAGAGAAGGAGGAGAAGCATCTGAGATCCCTGTTGATATATCTGTATTGTCCAACATGCGTCAGTTTGTCAAGTACAGCCGTTTTAAGCAATTTGCTCTGAGGGCTTTAGCAAGTACACTAAAAGAGGAAGAACTAGCAGATCTGAAGGACCAGTTCGATGCAATTGATGTTGATAAAAGTGGATCAATTAGTATTGAGGAAATGCGGCATGCCCTTGCAAAGGATCTTCCTTGGAGATTGAAGGGCCCCCGTGTTCTCGAGATTATCCAAGCAATCGACAGCAACACTGATGGTCTTGTGGACTTTGAAGAGTTTGTAGCAGCAACCCTCCATATACATCAAATGGCTGAGCTTGACTCTGAAAGGTGGGGCCTACGCTGCCAGGCTGCTTTCAGCAAATTTGATCTGGATGGTGACGGATACATCACTCCAGATGAACTCAGAATGGTGCAGCACACTGGCTTGAAGGGTTCCATCGAGCCATTGCTGGAGGAGGCCGACATCGACAAAGACGGGAGAATAAGCTTGTCGGAGTTCCGCAAGCTCCTGCGGACAGCGAGCATGAGCAACCTTCCCAGTCCAAGAGGACCTCCAAATCCACAACCCCTGTGAACGTCGGTAAGAACCGAGATGAGACGTCGTAAATTAGTCTTGTAAAGGTTGTCTTGTTCCTGTCCGCGTGCCATGTGATGTTGAGGGTGTCTTGAGTTTTTAGCATCCAGGTTCCGTGTGCAGCATGCAGATAAAGTTTCGGTGTAGTACGGAGTACATACATATTTTTTTCAAAAAAAAATGTTACAAGCAGAAATGCTGTTCTAGTCTCCCATTCTCCTCCTTGTACATAATTCTATATATGTATGATTTGATTGCACGTATTCATCGCAATAAAGTTGCCTGGGATTGCATGATCATGTGAACTTGTGTACTAGATGTAGATGGTGATATTGCAAGGCCATCTAGCTAGCTAGCAGCTGTAATACTAGTTCAGAGCTAAGCAACTTTCAAATCTATTTGAGTATTTGTCTATGCAGTGTTGATTTGTTTGAGGTTT
>LOC_Os02g03410.2
ACCTTCCTCTCCACCTTCCTCTTCCTCCCGACCAAACAACCTCCCTCCTCCTCCTCCACCTCGTCGTCGTCTCCGCCGCGCTGTCTCCTCCTCTATATACCCTCACCCACACTCGCACGCGCAAACCCAACCCCCGCATATGCTTAACTCCCCAACCCAAACCCTCCTCCTCCCCCATGGGCGCGTGCTTCTCATCCCACACTGCGACCGCCGCCGCCGATGGCGGGAGCGGGAAGCGGCAGCAGCGGAAGGGGGATCACAAGGGGAAGCTCCCCGATGGCGGCGGCGGCGAGAAGGAGAAGGAGGCGGCGCGGGTGGAGTTCGGGTACGAGAGGGACTTCGAGGGGAGGTACCAGGTCGGGAGGCTGCTCGGCCACGGCCAGTTCGGCTACACCTTCGCCGCCACCGACCGGGCCTCCGGTGACCGCGTCGCCGTCAAGCGCATCGACAAGGCCAAGATGGTTCGCCCTGTTGCTGTGGAGGATGTAAAGAGAGAAGTGAAGATTCTTAAAGAACTTAAAGGCCATGAGAATATTGTTCACTTCTACAATGCGTTTGAAGATGACTCATATGTATATATTGTGATGGAACTATGTGAGGGTGGTGAACTATTGGACCGGATTTTGGCAAAAAAGAACAGCCGTTATAGTGAGAAAGATGCTGCAGTGGTGGTGCGGCAGATGCTCAAAGTGGCAGCTGAGTGCCATCTGCATGGGCTAGTTCACCGAGATATGAAGCCCGAGAACTTCCTTTTCAAATCAACCAAGGAGGACTCACCTTTAAAGGCAACAGATTTTGGTCTGTCAGACTTCATAAAACCAGGGAAAAAGTTTCACGATATAGTTGGCAGTGCCTATTATGTAGCACCAGAAGTTTTAAAACGACGGTCTGGCCCTGAGTCAGATGTTTGGAGCATAGGAGTCATAACTTATATTTTGCTCTGTGGGAGACGCCCTTTTTGGAATAAGACAGAGGATGGCATATTCAGAGAGGTACTAAGAAACAAGCCTGATTTTCGTAAGAAGCCTTGGCCAGGCATCAGTTCAGGTGCTAAAGATTTCGTTAAAAAGTTACTTGTAAAGAACCCAAGGGCAAGATTAACCGCTGCTCAAGCTCTCTCGCATCCATGGGTAAGAGAAGGAGGAGAAGCATCTGAGATCCCTGTTGATATATCTGTATTGTCCAACATGCGTCAGTTTGTCAAGTACAGCCGTTTTAAGCAATTTGCTCTGAGGGCTTTAGCAAGTACACTAAAAGAGGAAGAACTAGCAGATCTGAAGGACCAGTTCGATGCAATTGATGTTGATAAAAGTGGATCAATTAGTATTGAGGAAATGCGGCATGCCCTTGCAAAGGATCTTCCTTGGAGATTGAAGGGCCCCCGTGTTCTCGAGATTATCCAAGCAATCGACAGCAACACTGATGGTCTTGTGGACTTTGAAGAGTTTGTAGCAGCAACCCTCCATATACATCAAATGGCTGAGCTTGACTCTGAAAGGTGGGGCCTACGCTGCCAGGCTGCTTTCAGCAAATTTGATCTGGATGGTGACGGATACATCACTCCAGATGAACTCAGAATGGTGCAGCACACTGGCTTGAAGGGTTCCATCGAGCCATTGCTGGAGGAGGCCGACATCGACAAAGACGGGAGAATAAGCTTGTCGGAGTTCCGCAAGCTCCTGCGGACAGCGAGCATGAGCAACCTTCCCAGTCCAAGAGGACCTCCAAATCCACAACCCCTGTGAACGTCGGTAAGAACCGAGATGAGACGTCGTAAATTAGTCTTGTAAAGGTTGTCTTGTTCCTGTCCGCGTGCCATGTGATGTTGAGGGTGTCTTGAGTTTTTAGCATCCAGGTTCCGTGTGCAGCATGCAGATAAAGTTTCGGTGTAGTACGGAGTACATACATATTTTTTTCAAAAAAAAATGTTACAAGCAGAAATGCTGTTCTAGTCTCCCATTCTCCTCCTTGTACATAATTCTATATATGTATGATTTGATTGCACGTATTCATCGCAATAAAGTTGCCTGGGATTGCATGATCATGTGAACTTGTGTACTAGATGTAGATGGTGATATTGCAAGGCCATCTAGCTAGCTAGCAGCTGTAATACTAGTTCAGAGCTAAGCAACTTTCAAATCTATTTGAGTATTTGTCTATGCAGTGTTGATTTGTTTGAGGTTT

CDS Sequence

>LOC_Os02g03410.1
ATGGGCGCGTGCTTCTCATCCCACACTGCGACCGCCGCCGCCGATGGCGGGAGCGGGAAGCGGCAGCAGCGGAAGGGGGATCACAAGGGGAAGCTCCCCGATGGCGGCGGCGGCGAGAAGGAGAAGGAGGCGGCGCGGGTGGAGTTCGGGTACGAGAGGGACTTCGAGGGGAGGTACCAGGTCGGGAGGCTGCTCGGCCACGGCCAGTTCGGCTACACCTTCGCCGCCACCGACCGGGCCTCCGGTGACCGCGTCGCCGTCAAGCGCATCGACAAGGCCAAGATGGTTCGCCCTGTTGCTGTGGAGGATGTAAAGAGAGAAGTGAAGATTCTTAAAGAACTTAAAGGCCATGAGAATATTGTTCACTTCTACAATGCGTTTGAAGATGACTCATATGTATATATTGTGATGGAACTATGTGAGGGTGGTGAACTATTGGACCGGATTTTGGCAAAAAAGAACAGCCGTTATAGTGAGAAAGATGCTGCAGTGGTGGTGCGGCAGATGCTCAAAGTGGCAGCTGAGTGCCATCTGCATGGGCTAGTTCACCGAGATATGAAGCCCGAGAACTTCCTTTTCAAATCAACCAAGGAGGACTCACCTTTAAAGGCAACAGATTTTGGTCTGTCAGACTTCATAAAACCAGGGAAAAAGTTTCACGATATAGTTGGCAGTGCCTATTATGTAGCACCAGAAGTTTTAAAACGACGGTCTGGCCCTGAGTCAGATGTTTGGAGCATAGGAGTCATAACTTATATTTTGCTCTGTGGGAGACGCCCTTTTTGGAATAAGACAGAGGATGGCATATTCAGAGAGGTACTAAGAAACAAGCCTGATTTTCGTAAGAAGCCTTGGCCAGGCATCAGTTCAGGTGCTAAAGATTTCGTTAAAAAGTTACTTGTAAAGAACCCAAGGGCAAGATTAACCGCTGCTCAAGCTCTCTCGCATCCATGGGTAAGAGAAGGAGGAGAAGCATCTGAGATCCCTGTTGATATATCTGTATTGTCCAACATGCGTCAGTTTGTCAAGTACAGCCGTTTTAAGCAATTTGCTCTGAGGGCTTTAGCAAGTACACTAAAAGAGGAAGAACTAGCAGATCTGAAGGACCAGTTCGATGCAATTGATGTTGATAAAAGTGGATCAATTAGTATTGAGGAAATGCGGCATGCCCTTGCAAAGGATCTTCCTTGGAGATTGAAGGGCCCCCGTGTTCTCGAGATTATCCAAGCAATCGACAGCAACACTGATGGTCTTGTGGACTTTGAAGAGTTTGTAGCAGCAACCCTCCATATACATCAAATGGCTGAGCTTGACTCTGAAAGGTGGGGCCTACGCTGCCAGGCTGCTTTCAGCAAATTTGATCTGGATGGTGACGGATACATCACTCCAGATGAACTCAGAATGGTGCAGCACACTGGCTTGAAGGGTTCCATCGAGCCATTGCTGGAGGAGGCCGACATCGACAAAGACGGGAGAATAAGCTTGTCGGAGTTCCGCAAGCTCCTGCGGACAGCGAGCATGAGCAACCTTCCCAGTCCAAGAGGACCTCCAAATCCACAACCCCTGTGA
>LOC_Os02g03410.2
ATGGGCGCGTGCTTCTCATCCCACACTGCGACCGCCGCCGCCGATGGCGGGAGCGGGAAGCGGCAGCAGCGGAAGGGGGATCACAAGGGGAAGCTCCCCGATGGCGGCGGCGGCGAGAAGGAGAAGGAGGCGGCGCGGGTGGAGTTCGGGTACGAGAGGGACTTCGAGGGGAGGTACCAGGTCGGGAGGCTGCTCGGCCACGGCCAGTTCGGCTACACCTTCGCCGCCACCGACCGGGCCTCCGGTGACCGCGTCGCCGTCAAGCGCATCGACAAGGCCAAGATGGTTCGCCCTGTTGCTGTGGAGGATGTAAAGAGAGAAGTGAAGATTCTTAAAGAACTTAAAGGCCATGAGAATATTGTTCACTTCTACAATGCGTTTGAAGATGACTCATATGTATATATTGTGATGGAACTATGTGAGGGTGGTGAACTATTGGACCGGATTTTGGCAAAAAAGAACAGCCGTTATAGTGAGAAAGATGCTGCAGTGGTGGTGCGGCAGATGCTCAAAGTGGCAGCTGAGTGCCATCTGCATGGGCTAGTTCACCGAGATATGAAGCCCGAGAACTTCCTTTTCAAATCAACCAAGGAGGACTCACCTTTAAAGGCAACAGATTTTGGTCTGTCAGACTTCATAAAACCAGGGAAAAAGTTTCACGATATAGTTGGCAGTGCCTATTATGTAGCACCAGAAGTTTTAAAACGACGGTCTGGCCCTGAGTCAGATGTTTGGAGCATAGGAGTCATAACTTATATTTTGCTCTGTGGGAGACGCCCTTTTTGGAATAAGACAGAGGATGGCATATTCAGAGAGGTACTAAGAAACAAGCCTGATTTTCGTAAGAAGCCTTGGCCAGGCATCAGTTCAGGTGCTAAAGATTTCGTTAAAAAGTTACTTGTAAAGAACCCAAGGGCAAGATTAACCGCTGCTCAAGCTCTCTCGCATCCATGGGTAAGAGAAGGAGGAGAAGCATCTGAGATCCCTGTTGATATATCTGTATTGTCCAACATGCGTCAGTTTGTCAAGTACAGCCGTTTTAAGCAATTTGCTCTGAGGGCTTTAGCAAGTACACTAAAAGAGGAAGAACTAGCAGATCTGAAGGACCAGTTCGATGCAATTGATGTTGATAAAAGTGGATCAATTAGTATTGAGGAAATGCGGCATGCCCTTGCAAAGGATCTTCCTTGGAGATTGAAGGGCCCCCGTGTTCTCGAGATTATCCAAGCAATCGACAGCAACACTGATGGTCTTGTGGACTTTGAAGAGTTTGTAGCAGCAACCCTCCATATACATCAAATGGCTGAGCTTGACTCTGAAAGGTGGGGCCTACGCTGCCAGGCTGCTTTCAGCAAATTTGATCTGGATGGTGACGGATACATCACTCCAGATGAACTCAGAATGGTGCAGCACACTGGCTTGAAGGGTTCCATCGAGCCATTGCTGGAGGAGGCCGACATCGACAAAGACGGGAGAATAAGCTTGTCGGAGTTCCGCAAGCTCCTGCGGACAGCGAGCATGAGCAACCTTCCCAGTCCAAGAGGACCTCCAAATCCACAACCCCTGTGA

Protein Sequence

>LOC_Os02g03410.1
MGACFSSHTATAAADGGSGKRQQRKGDHKGKLPDGGGGEKEKEAARVEFGYERDFEGRYQVGRLLGHGQFGYTFAATDRASGDRVAVKRIDKAKMVRPVAVEDVKREVKILKELKGHENIVHFYNAFEDDSYVYIVMELCEGGELLDRILAKKNSRYSEKDAAVVVRQMLKVAAECHLHGLVHRDMKPENFLFKSTKEDSPLKATDFGLSDFIKPGKKFHDIVGSAYYVAPEVLKRRSGPESDVWSIGVITYILLCGRRPFWNKTEDGIFREVLRNKPDFRKKPWPGISSGAKDFVKKLLVKNPRARLTAAQALSHPWVREGGEASEIPVDISVLSNMRQFVKYSRFKQFALRALASTLKEEELADLKDQFDAIDVDKSGSISIEEMRHALAKDLPWRLKGPRVLEIIQAIDSNTDGLVDFEEFVAATLHIHQMAELDSERWGLRCQAAFSKFDLDGDGYITPDELRMVQHTGLKGSIEPLLEEADIDKDGRISLSEFRKLLRTASMSNLPSPRGPPNPQPL*
>LOC_Os02g03410.2
MGACFSSHTATAAADGGSGKRQQRKGDHKGKLPDGGGGEKEKEAARVEFGYERDFEGRYQVGRLLGHGQFGYTFAATDRASGDRVAVKRIDKAKMVRPVAVEDVKREVKILKELKGHENIVHFYNAFEDDSYVYIVMELCEGGELLDRILAKKNSRYSEKDAAVVVRQMLKVAAECHLHGLVHRDMKPENFLFKSTKEDSPLKATDFGLSDFIKPGKKFHDIVGSAYYVAPEVLKRRSGPESDVWSIGVITYILLCGRRPFWNKTEDGIFREVLRNKPDFRKKPWPGISSGAKDFVKKLLVKNPRARLTAAQALSHPWVREGGEASEIPVDISVLSNMRQFVKYSRFKQFALRALASTLKEEELADLKDQFDAIDVDKSGSISIEEMRHALAKDLPWRLKGPRVLEIIQAIDSNTDGLVDFEEFVAATLHIHQMAELDSERWGLRCQAAFSKFDLDGDGYITPDELRMVQHTGLKGSIEPLLEEADIDKDGRISLSEFRKLLRTASMSNLPSPRGPPNPQPL*