Gene Details:

Functional Descriptions:

  • OsCYP2, a chaperone involved in degradation of auxin-responsive proteins, plays crucial roles in rice lateral root initiation.
  • Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed.
  • Heterologous expression of a salinity and developmentally regulated rice cyclophilin gene (OsCYP2) in E. coli and S. cerevisiae confers tolerance towards multiple abiotic stresses.
  • Our results suggest that OsCYP2 participates in auxin signal transduction by interacting with OsSGT1.
  • Expression of OsCYP2 enhances the ability of Escherichia coli to survive under diverse abiotic stresses viz.
  • Based on these results, we propose that OsCYP2 may serve as a ‘suitable candidate’ for raising transgenic plants for enhanced multiple abiotic stress tolerance.
  • OsCYP2 was able to complement the yeast mutant lacking native Cyp2 and also improved the growth of wild type yeast under above-mentioned stress conditions.
  • Ectopic expression of OsCyp2-P imparted multiple abiotic stress tolerance to transgenic tobacco plants as evidenced by higher root length, shoot length, chlorophyll content, and K(+)/Na(+) ratio under stress conditions.
  • Expression of a cyclophilin OsCyp2-P isolated from a salt-tolerant landrace of rice in tobacco alleviates stress via ion homeostasis and limiting ROS accumulation.
  • OsCyp2-P operates via reactive oxygen species (ROS) scavenging and ion homeostasis and thus is a promising candidate gene for enhancing multiple abiotic stress tolerance in crop plants.
  • The overall results suggest the explicit role of OsCyp2-P in bestowing multiple abiotic stress tolerance at the whole plant level.
  • Publicly available massively parallel signature sequencing (MPSS) and microarray data, besides our quantitative real time PCR (qRT-PCR) data suggest that transcript abundance of OsCyp2-P is regulated under different stress conditions in a developmental and organ specific manner.
  • Moreover, knockdown of the OsIAA11 expression partially rescues the LRT2 mutant phenotype in lateral root development.
  • To better understand the molecular mechanisms underlying lateral root formation, an auxin-responsive gene OsCYP2 (Os02g0121300) was characterized from rice.
  • T2 OsZFP-RNAi lines had significantly fewer lateral roots than did wild-type plants, which suggests a role for OsCYP2 and OsZFP in regulating lateral root development.
  • These findings imply that OsCYP2 and OsZFP participate in IAA signal pathways controlling lateral root development.
  • Yeast two-hybrid and glutathione S-transferase pull-down results confirmed that OsCYP2 interacted with a C2HC-type zinc finger protein (OsZFP, Os01g0252900) which is located in the rice nucleus.
  • In rice, a tryptophan-proline (W-P) cis-trans switch in transcription repressor protein OsIAA11 along with its associated cyclophilin LRT2 are essential components in a negative feedback gene regulation circuit that controls lateral root initiation in response to the plant hormone auxin.
  • Here we present NMR studies that determine and independently validate these parameters for LRT2 catalysis of the W-P motif in OsIAA11, providing predictive power for understanding the role of this switch in the auxin-responsive circuit and the resulting lateral rootless phenotype in rice.
  • Binding of the transcription factor MYC2-like to the ABRE of the OsCYP2 promoter enhances salt tolerance in Oryza sativa.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os02g02890.1
    TCAGGCCACGCAGCGATCTGAAGTGAAACAGCAAAAAAAATCAAACAAAAAGAAAAAATATTCCCCATCTGTGAAATTCGCAAAACCCTAGCGCGGCGGCGATGTCGAACACGAGGGTGTTCTTCGACATGACCGTCGGCGGAGCTCCGGCGGGGCGGATCGTGATGGAGCTGTACGCGAAGGACGTGCCGCGGACGGCGGAGAACTTCCGCGCGCTCTGCACCGGCGAGAAGGGCGTGGGCAAGAGCGGCAAGCCGCTGCACTACAAGGGGAGCACCTTCCACCGCGTGATCCCGGAGTTCATGTGCCAGGGCGGCGACTTCACCCGCGGCAACGGCACGGGAGGGGAGTCGATCTACGGCGAGAAGTTCGCCGACGAGGTGTTCAAGTTCAAGCACGACAGCCCCGGCATCCTGTCCATGGCGAACGCCGGGCCCAACACTAACGGGTCCCAGTTCTTCATCTGCACCGTGCCCTGCAGCTGGCTGGACGGGAAGCACGTCGTGTTCGGCCGCGTCGTCGAGGGCATGGACGTCGTCAAGGCCATCGAGAAGGTGGGATCCCGCGGCGGGAGCACCGCCAAGCCGGTCGTCATCGCCGACTGCGGCCAGCTCTCCTAGATCTGTGCTGTTCCCCTTCGCCTTTCGCCAGTATCAGTCGTCTTGAGTCGTCGAGTCCCTAAATAAGGAGGAGGTGGTGGTGGTGTTAGTCTTTTTATGAGTTCGTGTCGTGTTGGTGAGATGAGATCGCCCATGGTTTGGTTGGATTAGGCGGAGTTCTTGGATCGATTCGGTGGAGTTGGATCTGCGATCCTTCTTGGGGTTGGTTTTAAATCTTAATTCGTGTCGCTGCTTCTATGATATCGCTATCAATCAATGAGAACATTTGGGATCCGTGGATTTTCTGTGATCTTACACCTGTGTGGTTGTTTGTTCTTGCGACAGTACTAACGTTCTTGCGATTAAAGTAGATTAAACTATCTTCGTGAGATGAGCGGTTTGTCGCCGCGTGATGCCTGACTTTGATGTGATTTGTGGGACTTCGTCTTATCCATTTGGTTATTATTATTTTTTTATTT
CDS Sequence
  • >LOC_Os02g02890.1
    ATGTCGAACACGAGGGTGTTCTTCGACATGACCGTCGGCGGAGCTCCGGCGGGGCGGATCGTGATGGAGCTGTACGCGAAGGACGTGCCGCGGACGGCGGAGAACTTCCGCGCGCTCTGCACCGGCGAGAAGGGCGTGGGCAAGAGCGGCAAGCCGCTGCACTACAAGGGGAGCACCTTCCACCGCGTGATCCCGGAGTTCATGTGCCAGGGCGGCGACTTCACCCGCGGCAACGGCACGGGAGGGGAGTCGATCTACGGCGAGAAGTTCGCCGACGAGGTGTTCAAGTTCAAGCACGACAGCCCCGGCATCCTGTCCATGGCGAACGCCGGGCCCAACACTAACGGGTCCCAGTTCTTCATCTGCACCGTGCCCTGCAGCTGGCTGGACGGGAAGCACGTCGTGTTCGGCCGCGTCGTCGAGGGCATGGACGTCGTCAAGGCCATCGAGAAGGTGGGATCCCGCGGCGGGAGCACCGCCAAGCCGGTCGTCATCGCCGACTGCGGCCAGCTCTCCTAG
Protein Sequence
  • >LOC_Os02g02890.1
    MSNTRVFFDMTVGGAPAGRIVMELYAKDVPRTAENFRALCTGEKGVGKSGKPLHYKGSTFHRVIPEFMCQGGDFTRGNGTGGESIYGEKFADEVFKFKHDSPGILSMANAGPNTNGSQFFICTVPCSWLDGKHVVFGRVVEGMDVVKAIEKVGSRGGSTAKPVVIADCGQLS*