Information report for TDR
Gene Details
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Functional Descriptions
- Furthermore, additional evidence is provided that the expression of OsC6 is positively regulated by a basic helix-loop-helix transcription factor, Tapetum Degeneration Retardation (TDR).
- While the GAMYB expression was not obviously changed in TDR and udt1-1, and no apparent expression fold change of UDT1 in TDR and gamyb-4, suggesting that TDR may act downstream of GAMYB and UDT1, and GAMYB and UDT1 work in parallel to regulate rice early anther development.
- The expression levels of Osc6, OsRAFTIN and TDR, which are tapetum-specific genes, were unaffected by high temperatures.
- These results revealed that the expression of TDR (TAPETUM DEGENERATION RETARDATION), a tapetal cell death regulator, was downregulated in gamyb-4 and udt1 (undeveloped tapetum1).
- Here, we report on the function of the rice tapetum-expressing TDR (Tapetum Degeneration Retardation) gene in aliphatic metabolism and its regulatory role during rice pollen development.
- The observations of transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses suggested that pollen wall formation was significantly altered in the TDR mutant.
- Microarray data revealed that a group of genes putatively involved in lipid transport and metabolism were significantly altered in the TDR mutant, indicating the critical role of TDR in the formation of the pollen wall.
- In addition to its function in promoting tapetum PCD, TDR possibly plays crucial regulatory roles in several basic biological processes during rice pollen development.
- The contents of aliphatic compositions of anther were greatly changed in the TDR mutant revealed by GC-MS (gas chromatography-mass spectrometry) testing, particularly less accumulated in fatty acids, primary alcohols, alkanes and alkenes, and an abnormal increase in secondary alcohols with carbon lengths from C29 to C35 in TDR.
- Extra granule-like structures were observed on the inner surface of the TDR tapetal layer when the expression of OsC6 was driven by the TDR promoter compared with the TDR mutant.
- We report the isolation and characterization of a rice (Oryza sativa) male sterile mutant tapetum degeneration retardation (TDR), which exhibits degeneration retardation of the tapetum and middle layer as well as collapse of microspores.
- The TDR gene is preferentially expressed in the tapetum and encodes a putative basic helix-loop-helix protein, which is likely localized to the nucleus.
- These results indicate that TDR is a key component of the molecular network regulating rice tapetum development and degeneration.
- In addition, we uncover a possible mechanism by which GA regulates male fertility: SLENDER RICE1 (SLR1) interacts with and sequesters two critical transcription factors for tapetum development, UNDEVELOPED TAPETUM1 (UDT1), and TAPETUM DEGENERATION RETARDATION (TDR), and GA alleviates the sequestration by SLR1, thus allowing UDT1 and TDR to activate transcription.
Functional Keywords
- transcription-factor , anther , tapetum , cell-death , pollen , temperature , tapetal , microspore , sterile , anther-development , tapetum-degeneration , ga , GA , male-fertility
Literature and News
- Identification of gamyb-4 and analysis of the regulatory role of GAMYB in rice anther development . DOI: 10.1111/j.1744-7909.2010.00959.x ; PMID: 20590996
- Tapetum degeneration retardation is critical for aliphatic metabolism and gene regulation during rice pollen development . DOI: 10.1093/mp/ssn028 ; PMID: 19825565
- High temperatures cause male sterility in rice plants with transcriptional alterations during pollen development . DOI: 10.1093/pcp/pcp135 ; PMID: 19808807
- OsC6, encoding a lipid transfer protein, is required for postmeiotic anther development in rice . DOI: 10.1104/pp.110.158865 ; PMID: 20610705
- The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development . DOI: 10.1105/tpc.106.044107 ; PMID: 17138695
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice . DOI: 10.1186/s12284-020-00451-y ; PMID: 33409767
- WRKY53 negatively regulates rice cold tolerance at the booting stage by fine-tuning anther gibberellin levels . DOI: 10.1093/plcell/koac253 ; PMID: 35972376
Gene Resources
- UniProt: Q6YUS3
- EMBL: AP004078, AP005851, AP008208
- AlphaFoldDB: Q6YUS3
- EnsemblPlants: Os02t0120500-01, Os02t0120500-02
- Gramene: Os02t0120500-01, Os02t0120500-02
- KEGG: dosa:Os02g0120500
- Orthologous matrix: HPRTKAC
- InterPro: IPR011598, IPR036638, IPR051358
- PANTHER: PTHR31945, PTHR31945:SF11
- SUPFAM: SSF47459
- PROSITE: PS50888
- Gene3D: 4.10.280.10
- OrthoDB: Q6YUS3
- SWISS-MODEL: Q6YUS3
- Conserved Domain Database: cd11443
- eggNOG: ENOG502QQUB
Sequences
cDNA Sequence
- >LOC_Os02g02820.1
AGTATCATACATAATTTGACAGCCATTTCCGAAGACTTGGTATCAATACATCATCAGATAGCCATGGCTAAATTCAGAGGAACACACACATGTGAACTATATATATGCATGATGCAATAGTCCTTCTGTACAAAATGATTCTTCAGTTACCAATAGCTATCAGCTGATGCCTCTCTCTTCCATCTGTTGATCACTGCCACAGTTCGTCAGTTAGTTTGAAGCGATCTCAGGAAGACAGAAGGGCAAGGGTGATCACCACATGGGAAGAGGAGACCACCTGCTGATGAAGAACAGCAATGCTGCTGCAGCTGCAGCTGCTGTCAATGGAGGTGGCACCAGTTTGGATGCTGCATTGAGGCCTCTAGTTGGTTCAGATGGCTGGGATTACTGCATCTACTGGAGGCTCTCTCCTGATCAGAGGTTCTTGGAGATGACAGGATTCTGCTGCAGCAGTGAGCTTGAAGCACAGGTCTCAGCACTGCTGGACCTGCCTTCTTCAATCCCACTGGACTCCTCCTCCATAGGGATGCATGCGCAGGCATTGCTGTCGAACCAGCCGATCTGGCAGAGCAGCAGTGAGGAGGAGGAGGCTGATGGCGGCGGTGGCGCCAAGACGCGGCTGCTGGTCCCCGTCGCCGGCGGCCTCGTCGAGCTCTTCGCGTCGAGATATATGGCGGAGGAGCAGCAGATGGCGGAGCTTGTCATGGCGCAGTGCGGCGGCGGCGGCGCCGGGGACGACGGTGGGGGGCAGGCGTGGCCGCCGCCGGAGACGCCCAGCTTCCAGTGGGACGGAGGCGCCGACGCGCAGAGGCTGATGTACGGCGGCTCGTCGCTGAACCTGTTCGACGCCGCCGCCGCCGACGACGACCCGTTCTTGGGTGGTGGTGGTGGTGACGCCGTGGGCGACGAGGCGGCGGCGGCGGGCGCGTGGCCGTACGCGGGGATGGCGGTGAGCGAGCCGTCGGTGGCGGTGGCGCAGGAGCAGATGCAGCACGCGGCGGGCGGCGGCGTGGCGGAGTCCGGGTCGGAGGGGAGGAAGCTGCATGGCGGTGACCCGGAGGACGACGGCGACGGCGAGGGGCGCTCCGGCGGCGCCAAGAGGCAGCAGTGCAAGAACCTCGAGGCGGAGAGGAAGCGGAGGAAGAAGCTCAATGGCCACCTCTACAAGCTCCGCTCGCTCGTCCCAAACATCACCAAGATGGATCGCGCGTCGATTCTTGGAGACGCGATCGACTACATCGTGGGGTTGCAGAAGCAGGTGAAGGAGCTGCAGGACGAGCTGGAAGACAACCATGTCCACCATAAGCCGCCCGACGTGCTCATCGACCACCCGCCGCCGGCGAGCCTCGTCGGGCTCGACAACGACGACGCCTCGCCGCCCAACAGCCACCAACAGCAGCCGCCGCTCGCCGTTTCCGGCAGCAGCAGCAGGAGGAGTAACAAGGACCCAGCAATGACCGACGACAAGGTCGGCGGCGGCGGCGGCGGTGGGCACCGGATGGAGCCGCAGCTGGAGGTGCGTCAGGTGCAGGGGAACGAGCTGTTCGTCCAGGTGCTCTGGGAGCACAAGCCCGGCGGGTTCGTCCGCCTCATGGACGCCATGAACGCGCTCGGCCTCGAGGTCATCAACGTCAACGTCACCACCTACAAGACCCTCGTCCTCAACGTCTTCCGCGTCATGGTGAGGGACAGCGAGGTGGCGGTGCAGGCGGACAGGGTGAGGGACTCGCTGCTGGAGGTGACGCGGGAGACGTACCCCGGCGTGTGGCCGTCGCCGCAGGAGGAGGACGACGCCAAGTTCGACGGCGGCGACGGCGGGCAGGCTGCGGCGGCGGCGGCGGCGGCCGGTGGAGAGCACTACCACGACGAAGTCGGCGGCGGATACCATCAGCACCTGCATTACCTCGCGTTTGATTGATGATGATGATTGATTTTACCATCTACGTCTCACTACTAGTGTATACACACTCGAATTATTCGAGAAAGAAACATAATGCTACGTACTAGTAATAATGGATTTGATAAGTAGTTGTCTAGTATTTGTGATTAATGGCATATATGCATAATGACTTGATGACTGAAATGTGGCTAGCTAGAATTGTGGATACTTCTATCGAGTGTTTTTCCACTTTTGTTGGAAAAACTTTTGCTTGCCAA
CDS Sequence
- >LOC_Os02g02820.1
ATGGGAAGAGGAGACCACCTGCTGATGAAGAACAGCAATGCTGCTGCAGCTGCAGCTGCTGTCAATGGAGGTGGCACCAGTTTGGATGCTGCATTGAGGCCTCTAGTTGGTTCAGATGGCTGGGATTACTGCATCTACTGGAGGCTCTCTCCTGATCAGAGGTTCTTGGAGATGACAGGATTCTGCTGCAGCAGTGAGCTTGAAGCACAGGTCTCAGCACTGCTGGACCTGCCTTCTTCAATCCCACTGGACTCCTCCTCCATAGGGATGCATGCGCAGGCATTGCTGTCGAACCAGCCGATCTGGCAGAGCAGCAGTGAGGAGGAGGAGGCTGATGGCGGCGGTGGCGCCAAGACGCGGCTGCTGGTCCCCGTCGCCGGCGGCCTCGTCGAGCTCTTCGCGTCGAGATATATGGCGGAGGAGCAGCAGATGGCGGAGCTTGTCATGGCGCAGTGCGGCGGCGGCGGCGCCGGGGACGACGGTGGGGGGCAGGCGTGGCCGCCGCCGGAGACGCCCAGCTTCCAGTGGGACGGAGGCGCCGACGCGCAGAGGCTGATGTACGGCGGCTCGTCGCTGAACCTGTTCGACGCCGCCGCCGCCGACGACGACCCGTTCTTGGGTGGTGGTGGTGGTGACGCCGTGGGCGACGAGGCGGCGGCGGCGGGCGCGTGGCCGTACGCGGGGATGGCGGTGAGCGAGCCGTCGGTGGCGGTGGCGCAGGAGCAGATGCAGCACGCGGCGGGCGGCGGCGTGGCGGAGTCCGGGTCGGAGGGGAGGAAGCTGCATGGCGGTGACCCGGAGGACGACGGCGACGGCGAGGGGCGCTCCGGCGGCGCCAAGAGGCAGCAGTGCAAGAACCTCGAGGCGGAGAGGAAGCGGAGGAAGAAGCTCAATGGCCACCTCTACAAGCTCCGCTCGCTCGTCCCAAACATCACCAAGATGGATCGCGCGTCGATTCTTGGAGACGCGATCGACTACATCGTGGGGTTGCAGAAGCAGGTGAAGGAGCTGCAGGACGAGCTGGAAGACAACCATGTCCACCATAAGCCGCCCGACGTGCTCATCGACCACCCGCCGCCGGCGAGCCTCGTCGGGCTCGACAACGACGACGCCTCGCCGCCCAACAGCCACCAACAGCAGCCGCCGCTCGCCGTTTCCGGCAGCAGCAGCAGGAGGAGTAACAAGGACCCAGCAATGACCGACGACAAGGTCGGCGGCGGCGGCGGCGGTGGGCACCGGATGGAGCCGCAGCTGGAGGTGCGTCAGGTGCAGGGGAACGAGCTGTTCGTCCAGGTGCTCTGGGAGCACAAGCCCGGCGGGTTCGTCCGCCTCATGGACGCCATGAACGCGCTCGGCCTCGAGGTCATCAACGTCAACGTCACCACCTACAAGACCCTCGTCCTCAACGTCTTCCGCGTCATGGTGAGGGACAGCGAGGTGGCGGTGCAGGCGGACAGGGTGAGGGACTCGCTGCTGGAGGTGACGCGGGAGACGTACCCCGGCGTGTGGCCGTCGCCGCAGGAGGAGGACGACGCCAAGTTCGACGGCGGCGACGGCGGGCAGGCTGCGGCGGCGGCGGCGGCGGCCGGTGGAGAGCACTACCACGACGAAGTCGGCGGCGGATACCATCAGCACCTGCATTACCTCGCGTTTGATTGA
Protein Sequence
- >LOC_Os02g02820.1
MGRGDHLLMKNSNAAAAAAAVNGGGTSLDAALRPLVGSDGWDYCIYWRLSPDQRFLEMTGFCCSSELEAQVSALLDLPSSIPLDSSSIGMHAQALLSNQPIWQSSSEEEEADGGGGAKTRLLVPVAGGLVELFASRYMAEEQQMAELVMAQCGGGGAGDDGGGQAWPPPETPSFQWDGGADAQRLMYGGSSLNLFDAAAADDDPFLGGGGGDAVGDEAAAAGAWPYAGMAVSEPSVAVAQEQMQHAAGGGVAESGSEGRKLHGGDPEDDGDGEGRSGGAKRQQCKNLEAERKRRKKLNGHLYKLRSLVPNITKMDRASILGDAIDYIVGLQKQVKELQDELEDNHVHHKPPDVLIDHPPPASLVGLDNDDASPPNSHQQQPPLAVSGSSSRRSNKDPAMTDDKVGGGGGGGHRMEPQLEVRQVQGNELFVQVLWEHKPGGFVRLMDAMNALGLEVINVNVTTYKTLVLNVFRVMVRDSEVAVQADRVRDSLLEVTRETYPGVWPSPQEEDDAKFDGGDGGQAAAAAAAAGGEHYHDEVGGGYHQHLHYLAFD*