Gene Details:

Functional Descriptions:

  • OsHAC1:1 and OsHAC1;2 are predominantly expressed in roots, with OsHAC1;1 being abundant in the epidermis, root hairs and pericycle cells while OsHAC1;2 is abundant in the epidermis, outer layers of cortex and endodermis cells.
  • When grown under aerobic soil conditions overexpression of either OsHAC1;1 or OsHAC1;2 also decreased As accumulation in rice grain, whereas grain As increased in the knockout mutants.
  • We conclude that OsHAC1;1 and OsHAC1;2 are arsenate reductases that play an important role in restricting As accumulation in rice shoots and grain.
  • In contrast, overexpression of either OsHAC1;1 or OsHAC1;2 increased arsenite efflux, reduced As accumulation and enhanced arsenate tolerance.
  • OsHAC1;1 and OsHAC1;2 are able to complement an Escherichia coli mutant lacking the endogenous arsenate reductase and to reduce arsenate to arsenite.
  • Knocking out OsHAC1;1 or OsHAC1;2 decreased the reduction of arsenate to arsenite in roots, reducing arsenite efflux to the external medium.
  • OsHAC1;1 and OsHAC1;2 Function as Arsenate Reductases and Regulate Arsenic Accumulation.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os02g01220.1
    GTGCATCTCGCTACCTAACCTAGCTAACCTCCTCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTAGGCTAGCACTGCACGTGCCCAACTAACACATACATTCATCATATATACACCTCCTCCTCCTTCTTCCTCTAAGTTGTGTGTGCATATATAATCTACTAGTAGAGAGCAGGGCTAGGCTAGCAATTGCAATGGCGCCTCCCTATGAAACCAGCGCCGCCGGCTCTGAATCGCCGGTGCCGGTGGTAACGGTGGATGTGGCGGCGGCGAGCGACCTTATCACCTCGGCCGGCCACCGTTACGTCGACGTCAGGACAGAGGAGGAAATGAACAAGGGCCATCTACACAACTCCCTCAACGTGCCCTTCATGTTCGTCACGCCGCAAGGGAGGGAAAAGAATCCTCTGTTTGTGGAGCAGTTCTCGTCGCTGGTGAGCAAAGAGGAGCATGTGGTTGTGGGGTGCCAAAGCGGGAAGAGGTCGGAGCTAGCATGCGTTGATCTCCTTGAAGCAGGGTTCAAGAACGTGAAGAACATGGGAGGAGGCTACGCAGCGTGGCTCGACAATGGATTCCCCATAAACACTCCTCCTCATACAATGTATTAACGCTTTGCAGCACCGATGAGATGAGCGAGTGATAAACACGGACTCTACTGTATCTACTGTATCTCATTCCAACCCCTCTTCTCATCTGCCTGCTAGCCGCTGCTTCGTGTGCCCTGCCATGTATATGTATGTGCGTCCTGTTTTCCTGTTTGTTACTACTGGTATTATGGAGGCAGCCAATTGAATAAAATTGATCATCATGCATGCAATGCACGATGCGTCTGCAAATCTCCGGGATGACATCAATCAACCAAGTAGCTACACATGTTCAAGGACTTTATTATCTCTAAATCAGCATATTGTGGCCGGTTTGCCAATCCACCATAACAGTAGGCGTGAGGCAGAGCCTCTGGTTACAAATCCAAACGAACGACTCATACCAAAGGGGCAGGCCACCTTATGCAAGAATCATCAATCAAACGTTTGCTTCTCTTGCCATCTGCTATGTGCTGCGCCGTTGGCACTAAAGCATTGATTGATCGATCACCTCAAAAATTACAGTCTCACATCTCAGACTGCCGGTTGCAGGGCAAAGTGTAGAGAAACCAACATCCAAACAAATGAAGACCACACATCACACGGTTAGATGTGCAATTAGCTCTTTCTTTGGACGTCCATCGCCACAGCCAGATGGCGAGCTCCACAGGACAAACAAATTGATGACTACAATAAGTATGACTCAACCACGGTCCGCCCAAACTTCTGCTCAACATCCTTTGGCGTTTCAAT
CDS Sequence
  • >LOC_Os02g01220.1
    ATGGCGCCTCCCTATGAAACCAGCGCCGCCGGCTCTGAATCGCCGGTGCCGGTGGTAACGGTGGATGTGGCGGCGGCGAGCGACCTTATCACCTCGGCCGGCCACCGTTACGTCGACGTCAGGACAGAGGAGGAAATGAACAAGGGCCATCTACACAACTCCCTCAACGTGCCCTTCATGTTCGTCACGCCGCAAGGGAGGGAAAAGAATCCTCTGTTTGTGGAGCAGTTCTCGTCGCTGGTGAGCAAAGAGGAGCATGTGGTTGTGGGGTGCCAAAGCGGGAAGAGGTCGGAGCTAGCATGCGTTGATCTCCTTGAAGCAGGGTTCAAGAACGTGAAGAACATGGGAGGAGGCTACGCAGCGTGGCTCGACAATGGATTCCCCATAAACACTCCTCCTCATACAATGTATTAA
Protein Sequence
  • >LOC_Os02g01220.1
    MAPPYETSAAGSESPVPVVTVDVAAASDLITSAGHRYVDVRTEEEMNKGHLHNSLNVPFMFVTPQGREKNPLFVEQFSSLVSKEEHVVVGCQSGKRSELACVDLLEAGFKNVKNMGGGYAAWLDNGFPINTPPHTMY*