Information report for OsDREB1F;RCBF2
Gene Details
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Functional Descriptions
- Transgenic plants harbouring OsDREB1F gene led to enhanced tolerance to salt, drought, and low temperature in both rice and Arabidopsis.
- Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice.
- Here, a novel rice DREB transcription factor, OsDREB1F, was cloned and characterised via subtractive suppression hybridisation (SSH) from upland rice.
- The semi-quantitative RT-PCR (s-Q RT-PCR) analysis indicated the expression of RCBF2 gene was induced by cold, dehydration and high-salinity, but not by abscisic acid, and the transcription of RCBF2 gene accumulated primarily in rice immature seeds, growing point and shoots.
- Expression analysis revealed that OsDREB1F gene was induced by salt, drought, cold stresses, and also ABA application, but not by pathogen, wound, and H2O2.
- Analysis of the deduced RCBF2 amino acid sequence revealed that RCBF2 contained a conserved ethylene-responsive element binding protein (EREBP)/AP2 domain of 59 amino acids and a potential nuclear localization sequence.
- Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.
- A transcription factor RCBF2 which interacts with C-repeat/DRE was isolated from Oryza sativa L.
- Natural allelic diversity in OsDREB1F gene in the Indian wild rice germplasm led to ascertain its association with drought tolerance.
- Three coding SNPs and one haplotype identified in the OsDREB1F gene have potential to be associated with drought tolerance in rice.
- One OsDREB1F variant in the activation domain of OsDREB1F gene which led to conversion of aspartate amino acid to glutamate was found to be associated with drought tolerance.
Functional Keywords
- temperature , transcription-factor , salinity , drought , cold-stress , shoot , ethylene , ABA , seed , salt , drought-tolerance , tolerance
Literature and News
- Colinearity and similar expression pattern of rice DREB1s reveal their functional conservation in the cold-responsive pathway . DOI: 10.1371/journal.pone.0047275 ; PMID: 23077584
- Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L . DOI: 10.1007/s10529-006-9214-4 ; PMID: 17136305
- Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice . DOI: 10.1007/s11103-008-9340-6 ; PMID: 18470484
- Natural allelic diversity in OsDREB1F gene in the Indian wild rice germplasm led to ascertain its association with drought tolerance . DOI: 10.1007/s00299-015-1760-6 ; PMID: 25693492
- OsNAC45 is Involved in ABA Response and Salt Tolerance in Rice . DOI: 10.1186/s12284-020-00440-1 ; PMID: 33284415
Gene Resources
- UniProt: Q8S9Z5
- EMBL: AP003448, AP008207, AP014957
- AlphaFoldDB: Q8S9Z5
- EnsemblPlants: Os01t0968800-01
- Gramene: Os01t0968800-01
- KEGG: dosa:Os01g0968800
- Orthologous matrix: WDDHAYF
- InterPro: IPR001471, IPR016177, IPR036955
- PANTHER: PTHR31839, PTHR31839:SF42
- SUPFAM: SSF54171
- PROSITE: PS51032
- Gene3D: 3.30.730.10
- OrthoDB: Q8S9Z5
- SWISS-MODEL: Q8S9Z5
- Conserved Domain Database: cd00018
- eggNOG: ENOG502RZEY
Sequences
cDNA Sequence
- >LOC_Os01g73770.1
CCCTCCTCTCATCGCACCACCACGCCACTGAGCTCAAGCTAAGCTAAGTGCTAACCTAGGTGTTCGACCATGGACACCGAGGACACGTCGTCGGCTTCGTCCTCGTCGGTGTCGCCGCCGTCGTCGCCGGGCGGCGGGCACCACCACCGGCTGCCGCCGAAGCGGCGGGCGGGGCGGAAGAAATTCCGGGAGACGCGGCACCCGGTGTACCGCGGCGTGCGCGCGCGGGCGGGGGGGAGCAGGTGGGTGTGCGAGGTGCGCGAGCCGCAGGCGCAGGCGCGCATCTGGCTCGGCACCTACCCGACGCCGGAGATGGCGGCGCGCGCGCACGACGTCGCGGCCATCGCCCTCCGCGGCGAGCGCGGCGCCGAGCTCAACTTCCCGGACTCCCCCTCCACGCTCCCGCGCGCGCGCACGGCGTCGCCCGAGGACATCCGCCTCGCCGCCGCGCAGGCCGCCGAGCTGTACCGCCGCCCGCCGCCGCCGCTGGCATTGCCGGAGGATCCGCAGGAAGGCACGAGCGGCGGCGGCGCCACCGCCACCTCGGGGCGTCCGGCTGCCGTGTTCGTGGACGAGGACGCCATCTTCGACATGCCGGGGCTGATCGACGACATGGCGAGGGGGATGATGCTGACGCCGCCGGCGATTGGGAGATCTCTCGACGACTGGGCCGCCATCGACGACGACGATGACCATTACCACATGGACTACAAGCTATGGATGGACTGACATGTATACACACATATCGTTCGAGTATATATGCTTACAGATATAGATAATACTCCATCAGTTCCTACTAAATACGGAGTATATATTTTTAGATAAAGGATTTATATAAAACCCGGCTTCTACATCCCGAAGAATGTACACAGCCAATCCTACTAAATATATATAAGCACCTCTTGTTTGTTTAGAAAAAATGAGATTAAGGGAAAATA
CDS Sequence
- >LOC_Os01g73770.1
ATGGACACCGAGGACACGTCGTCGGCTTCGTCCTCGTCGGTGTCGCCGCCGTCGTCGCCGGGCGGCGGGCACCACCACCGGCTGCCGCCGAAGCGGCGGGCGGGGCGGAAGAAATTCCGGGAGACGCGGCACCCGGTGTACCGCGGCGTGCGCGCGCGGGCGGGGGGGAGCAGGTGGGTGTGCGAGGTGCGCGAGCCGCAGGCGCAGGCGCGCATCTGGCTCGGCACCTACCCGACGCCGGAGATGGCGGCGCGCGCGCACGACGTCGCGGCCATCGCCCTCCGCGGCGAGCGCGGCGCCGAGCTCAACTTCCCGGACTCCCCCTCCACGCTCCCGCGCGCGCGCACGGCGTCGCCCGAGGACATCCGCCTCGCCGCCGCGCAGGCCGCCGAGCTGTACCGCCGCCCGCCGCCGCCGCTGGCATTGCCGGAGGATCCGCAGGAAGGCACGAGCGGCGGCGGCGCCACCGCCACCTCGGGGCGTCCGGCTGCCGTGTTCGTGGACGAGGACGCCATCTTCGACATGCCGGGGCTGATCGACGACATGGCGAGGGGGATGATGCTGACGCCGCCGGCGATTGGGAGATCTCTCGACGACTGGGCCGCCATCGACGACGACGATGACCATTACCACATGGACTACAAGCTATGGATGGACTGA
Protein Sequence
- >LOC_Os01g73770.1
MDTEDTSSASSSSVSPPSSPGGGHHHRLPPKRRAGRKKFRETRHPVYRGVRARAGGSRWVCEVREPQAQARIWLGTYPTPEMAARAHDVAAIALRGERGAELNFPDSPSTLPRARTASPEDIRLAAAQAAELYRRPPPPLALPEDPQEGTSGGGATATSGRPAAVFVDEDAIFDMPGLIDDMARGMMLTPPAIGRSLDDWAAIDDDDDHYHMDYKLWMD*