Gene Details:

Functional Descriptions:

  • Transgenic plants harbouring OsDREB1F gene led to enhanced tolerance to salt, drought, and low temperature in both rice and Arabidopsis.
  • Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice.
  • Here, a novel rice DREB transcription factor, OsDREB1F, was cloned and characterised via subtractive suppression hybridisation (SSH) from upland rice.
  • The semi-quantitative RT-PCR (s-Q RT-PCR) analysis indicated the expression of RCBF2 gene was induced by cold, dehydration and high-salinity, but not by abscisic acid, and the transcription of RCBF2 gene accumulated primarily in rice immature seeds, growing point and shoots.
  • Expression analysis revealed that OsDREB1F gene was induced by salt, drought, cold stresses, and also ABA application, but not by pathogen, wound, and H2O2.
  • Analysis of the deduced RCBF2 amino acid sequence revealed that RCBF2 contained a conserved ethylene-responsive element binding protein (EREBP)/AP2 domain of 59 amino acids and a potential nuclear localization sequence.
  • Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.
  • A transcription factor RCBF2 which interacts with C-repeat/DRE was isolated from Oryza sativa L.
  • Natural allelic diversity in OsDREB1F gene in the Indian wild rice germplasm led to ascertain its association with drought tolerance.
  • Three coding SNPs and one haplotype identified in the OsDREB1F gene have potential to be associated with drought tolerance in rice.
  • One OsDREB1F variant in the activation domain of OsDREB1F gene which led to conversion of aspartate amino acid to glutamate was found to be associated with drought tolerance.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os01g73770.1
    CCCTCCTCTCATCGCACCACCACGCCACTGAGCTCAAGCTAAGCTAAGTGCTAACCTAGGTGTTCGACCATGGACACCGAGGACACGTCGTCGGCTTCGTCCTCGTCGGTGTCGCCGCCGTCGTCGCCGGGCGGCGGGCACCACCACCGGCTGCCGCCGAAGCGGCGGGCGGGGCGGAAGAAATTCCGGGAGACGCGGCACCCGGTGTACCGCGGCGTGCGCGCGCGGGCGGGGGGGAGCAGGTGGGTGTGCGAGGTGCGCGAGCCGCAGGCGCAGGCGCGCATCTGGCTCGGCACCTACCCGACGCCGGAGATGGCGGCGCGCGCGCACGACGTCGCGGCCATCGCCCTCCGCGGCGAGCGCGGCGCCGAGCTCAACTTCCCGGACTCCCCCTCCACGCTCCCGCGCGCGCGCACGGCGTCGCCCGAGGACATCCGCCTCGCCGCCGCGCAGGCCGCCGAGCTGTACCGCCGCCCGCCGCCGCCGCTGGCATTGCCGGAGGATCCGCAGGAAGGCACGAGCGGCGGCGGCGCCACCGCCACCTCGGGGCGTCCGGCTGCCGTGTTCGTGGACGAGGACGCCATCTTCGACATGCCGGGGCTGATCGACGACATGGCGAGGGGGATGATGCTGACGCCGCCGGCGATTGGGAGATCTCTCGACGACTGGGCCGCCATCGACGACGACGATGACCATTACCACATGGACTACAAGCTATGGATGGACTGACATGTATACACACATATCGTTCGAGTATATATGCTTACAGATATAGATAATACTCCATCAGTTCCTACTAAATACGGAGTATATATTTTTAGATAAAGGATTTATATAAAACCCGGCTTCTACATCCCGAAGAATGTACACAGCCAATCCTACTAAATATATATAAGCACCTCTTGTTTGTTTAGAAAAAATGAGATTAAGGGAAAATA
CDS Sequence
  • >LOC_Os01g73770.1
    ATGGACACCGAGGACACGTCGTCGGCTTCGTCCTCGTCGGTGTCGCCGCCGTCGTCGCCGGGCGGCGGGCACCACCACCGGCTGCCGCCGAAGCGGCGGGCGGGGCGGAAGAAATTCCGGGAGACGCGGCACCCGGTGTACCGCGGCGTGCGCGCGCGGGCGGGGGGGAGCAGGTGGGTGTGCGAGGTGCGCGAGCCGCAGGCGCAGGCGCGCATCTGGCTCGGCACCTACCCGACGCCGGAGATGGCGGCGCGCGCGCACGACGTCGCGGCCATCGCCCTCCGCGGCGAGCGCGGCGCCGAGCTCAACTTCCCGGACTCCCCCTCCACGCTCCCGCGCGCGCGCACGGCGTCGCCCGAGGACATCCGCCTCGCCGCCGCGCAGGCCGCCGAGCTGTACCGCCGCCCGCCGCCGCCGCTGGCATTGCCGGAGGATCCGCAGGAAGGCACGAGCGGCGGCGGCGCCACCGCCACCTCGGGGCGTCCGGCTGCCGTGTTCGTGGACGAGGACGCCATCTTCGACATGCCGGGGCTGATCGACGACATGGCGAGGGGGATGATGCTGACGCCGCCGGCGATTGGGAGATCTCTCGACGACTGGGCCGCCATCGACGACGACGATGACCATTACCACATGGACTACAAGCTATGGATGGACTGA
Protein Sequence
  • >LOC_Os01g73770.1
    MDTEDTSSASSSSVSPPSSPGGGHHHRLPPKRRAGRKKFRETRHPVYRGVRARAGGSRWVCEVREPQAQARIWLGTYPTPEMAARAHDVAAIALRGERGAELNFPDSPSTLPRARTASPEDIRLAAAQAAELYRRPPPPLALPEDPQEGTSGGGATATSGRPAAVFVDEDAIFDMPGLIDDMARGMMLTPPAIGRSLDDWAAIDDDDDHYHMDYKLWMD*