Gene Details:

• MSU gene ID: LOC_Os01g72930
• RAPdb gene ID: Os01g0959600
• Gene Symbol: EMP5 OsEMP5
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Here, we report the molecular characterization of the empty pericarp5 (EMP5) mutants in maize (Zea mays).
• EMP5 knockdown expression in transgenics resulted in slow growth and defective seeds.
• These results demonstrate that EMP5 encodes a PPR-DYW protein that is required for the editing of multiple transcripts in mitochondria, and the editing events, particularly the C-to-U editing at the rpl16-458 site, are critical to the mitochondrial functions and, hence, to seed development in maize.
• Null mutation of EMP5 results in abortion of embryo and endosperm development at early stages.
• Analysis of the mitochondrial transcripts revealed that loss of the EMP5 function abolishes the C-to-U editing of ribosomal protein L16 at the rpl16-458 site (100% edited in the wild type), decreases the editing at nine sites in NADH dehydrogenase9 (nad9), cytochrome c oxidase3 (cox3), and ribosomal protein S12 (rps12), and surprisingly increases the editing at five sites of ATP synthase F0 subunit a (atp6), apocytochrome b (cob), nad1, and rpl16.
• Analysis of the ortholog in rice (Oryza sativa) indicates that rice EMP5 has a conserved function in C-to-U editing of the rice mitochondrial rpl16-458 site.

Function-related keywords:

• pericarp ,  growth ,  seed-development ,  endosperm ,  seed ,  mitochondria

Literature:

• Empty pericarp5 encodes a pentatricopeptide repeat protein that is required for mitochondrial RNA editing and seed development in maize .  DOI:  10.1105/tpc.112.106781 ;  PMID:  23463776

Related News:

Gene Resources:

• NCBI ID: JX308938
• UniProt accessions:

Sequences: