Gene Details:

Functional Descriptions:

  • We isolated a cDNA for β-1,3-glucanase from rice anther and named it Oryza sativa glucanase 1 (Osg1).
  • RT-PCR analysis revealed that Osg1 transcripts were present in leaves, roots, and anthers.
  • Osg1 was found to be expressed throughout the plant and highly expressed in florets, leaf sheaths, and leaf blades.
  • The pollen mother cells appeared to be normal in Osg1-RI plants, but callose degradation was disrupted around the microspores in the anther locules of the Osg1-RI plants at the early microspore stage.
  • Investigations using real-time PCR, immunocytochemical analysis, and a GUS-reporter gene driven by the Osg1 promoter indicated that Osg1 was mainly expressed at the late meiosis, early microspore, and middle microspore stages in the florets.
  • The silencing of Osg1 resulted in male sterility.
  • A rice beta-1,3-glucanase gene Osg1 is required for callose degradation in pollen development.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g71930.1
    TCGGTTTCCTCGCCCCTTCTCCCTTCGGCTTTTTCTGGGCCCAGCTACCGCCGCCCAGCTACCGCCGCTGTGCTGCCGTCGGCCGGCGCCCCGGCCCTCTGCCGACCACCGAGCAGGAGCCGCGCGCGCATCCTCACTCCACCGGTGAGGCGCCTCTACCAGGAAGGGCCTATGCCACCTCGGAGCCCTGTCGACGCCTCCTCGCCGGAGACCCCGCCTGGCCCCCTCCGCCTCCCCACGAACCGCTGCTGTCTGATGATGCTCGTCGCCAGCAAGCCACGCCGCCCACGAAATCAAGGTCAGATTCGCTCCTGCTTATGTGATTTACATGAGTCATTACGGTGTGCAGATGCCAATTGAAAGCTGCCACATGCACGGCAAGATGAGCAAAGCAGTCAAGGTTCAGGACGATTGACCGTAAGCTTACTGAAAAAAGGAAATTGACATCTCATCGCAAACAAATCAGGCTCGCCCCCTTCGCCGTACGTACTACTAGCCTCACAAGTCACAAGTCACAAACCATCATCTTCACTAGTAGTAGAACCCAGATCGAGCAGCCATGTCTGTTCCTGACGAAAACCCGATTGCGGTGGAGAGGAAGATATTCTCAGCTGTGGATCGCATCCTGGTGAACATAAAAGTCCTAATAGGTGACATCGGGAGCAATCTGAAAAAGCTCCCCAAACAGAGCGACCACTCCTGCAACACCGGCGATGATGAGTTTGACGACGCCGTGGCGCTGCGGCACATCGAGGTCAACATCCATGCCGAACAGCTCGGCGCCGGAGTCAACCGTTTTCAGGACAGCATCGGCACGAAGCACCATGCCGAGGAGGAGTCGATGCCCTCATTGGCGGAGCAAGAAGACGAGCCCGCGCGCCCCATGTCTGGATTGCACGTCAGGCGCCTGCTTGACACCCTCGACGGGCAGCTGATGCAGTGCCTCCTCTGCCTTGCCATCTTCCCGCCAGGCGAGGCCATCAAGAAGAGGCTTCTCATCCACTGGTGGCTCGGGGAGGGGATAGTCCAATCGGCAGACGCAGGGAAGGAGCGCTTCAACGAGCTGTTCGATCGCGGCCTCGTTCAGCCCGCGCTACGCCGAGGCCACTGCCGACGTACACACTACTTCAGAGTGCACCCCGTGGTGCACAACCAGCTTGTGGAGAGCCTAGGTTTCTTCAGGTTTTTGGGTCATGGCAAAGGCAACGTCGACGAATAATGCCTTCCTGTCAGTATTCAATCTCGACATGGAGTACGTGAAGATTCATATCGCAAAATCGAGAATCACCAGAGTCGTGCAGCTTGGGCGGTGGAAGAGGTCAAGGAAGCACCACATCGAGCTGGTGGGAGACAACGACCTACTCAAAAAAGTTTTGGCGTGCAAGAACCTGAGGTACCTCAGCCTCAGGGGCATCTCCCTCATCGAGTCCATACCCGAGGCCATCGGCACGCTTGCCGAACTCCTCGTGCTCGACCTCAGGGCGTGCCACAACTTGGAGAAGTTACCGGGGTCTATCGGCTCGTTGCTGAAGCTAGAGTACCTGGACTTGTCCGAATGCTTCTTACTTGAAGAAATGCCCAAAGAGATCGGCGAGCTGTCCAAGCTGCAGGTCCTCAAGGGTTTTCTCGTTGGCAGTTCAAGAAAGAAGAGCAGCCCATGCCGTCTCGCCGACCTGGCCACAAAGGCGCAGAATCTACGGAAGCTAAACATTACCACTGGACGGCAATCTTTGGTGTGTGATGAAGATGAGCTCTGCCAATTGGCGAAATGCCAGCATCTTGAGTCCTTGACAATAACTTGGATAGGAGAGGCATCTACCGAGCCATATCTTCCTCTTCCTTCCAGCCTCACAAAGCTTGATCTCCGGCGTGCCCCTACCGCAAGCCTTCTTAATATCATTCATCCATCGACGTCGGTGAGCCTGAAACGGCTGTATATTAGGGGAGGGAAGCTCCGTACACTGGGGCAAGACGGCGGCTGGAACGTTGAGACACTGCGTGCTAGATGTCTCAATGATTTGGAGTGTGAGTGGAGTGAGCTCCATGGTTTGTTCCGAGAACTCCGTTTTGTGGAGATGTGGAGGTGTGCCCGCCTTAGTTTCTGGCCTTGCGATGGGCGGGGCGTATGGGACAAAGGATCTCCTTCTCTGGCTCGCCGATCCATTGGAGTCTGCTACGGAGTGAAAGGCAACAATCTGCCACCATGGCATGAAGTCGTTCAGCTATATGCCTCGAACAACATTCCTGCCATGAGGATATTCTATCCTCACCACGATGTTCTTGAGGCTCTGAGAGGCACAGGCATCGGTATCAGCCTCGACGTTGAGGGCCAATTCCTCCCATCCTTCGCCTCTGAACCCTCCGTTGCGGCTGCCTGGGTGAAGACCAATGTGCAAGCCTTTTACCCTGCAGTCTCATTCAAGTTCATCACAGTCGGCAACCAGGTTGCCCTGAGAGAGATGAGATACATCCTCCCAGCGATGCAGAATATCTATGCAGCGTTGTCAGCAGTAGGGCTGGATCACATCAAGGTGTCAACGTCGGTGAGAAGGGACGTGCTGGGTTTGTCCTATCCTCCCTCTGCTGGTGCTTTCTCCTCAGCGATGGAACAGTACATGGCACCTATTGTGCAGTTCTTGGCCAAGATTGGGGCTCCACTTCTGGCCAGTGTGTTCCCTTACTTTACTTACGTGCACAATCAGGAAGGCATTGATATCGATTACGCCCTTTTCACCTCCCCGGGAACTGTGGTGCAGGACGGTGAACATAGCTATCAGAACCTCTTCGATGCCATTGTGGATGCTCTGTATTCGGCGATGGAGAAGGTGGGTGGCTCAACCGTCAGAATAGTTGTCTCAGATAGCGGCTGGCCATCGGCTGGGGCTCCTGCAGCGACCAAAGATAATGCTAGGGCCTACGTCCAGAACCTGATTAACCATGTGAGCAAAGGGACACCCAAGAGGCCCGTACCTATAGAGACATACATTTTTGCCATGTTCAATGAGAACGAGAAAACCGGTGACGAGATAGAGAGGAACTTTGGGCTCTTTGAACCTGACAAGAGCCCTGTGTACCCAATCACCTTCTCTTAACCGAAAGGATAGTACAAGGAGGCAGCTGCTACAAAAACTCAACCACTTTTCACTGATCCTTCCATTTTGTTTTGGCATTTTATTAGGGGTTGCTATATAACGGAATCCCGTTTAGACGGATACTCGCGAGACAGACCTAGCCCAAAAATCGAGTGGGGGCATCTTATTAGAATTGATAGTGTGCTCAAAATTTTTTACTTAATTACACATTAGTAATACACTAAATATTAAATTTGAGTGGAGTCCTAGGACCCCACTCTACATACTGTGGATCCGCCCGTGCTCGCAAGGTGTCATCACCCTGATACATAGCTGATATCATCCTATCCGGACGAGATTCTATCCTGTAGCATTTCCGTTTTATTAATGCACCTTGAAACTTCCATTCTCTCATCCAGATAACCGTTAAACATTGTGTAATGTAACCGTCCATAATGAATAACGTTATTTCT
CDS Sequence
  • >LOC_Os01g71930.1
    ATGGCAAAGGCAACGTCGACGAATAATGCCTTCCTGTCAGTATTCAATCTCGACATGGAGTACGTGAAGATTCATATCGCAAAATCGAGAATCACCAGAGTCGTGCAGCTTGGGCGGTGGAAGAGGTCAAGGAAGCACCACATCGAGCTGGTGGGAGACAACGACCTACTCAAAAAAGTTTTGGCGTGCAAGAACCTGAGGTACCTCAGCCTCAGGGGCATCTCCCTCATCGAGTCCATACCCGAGGCCATCGGCACGCTTGCCGAACTCCTCGTGCTCGACCTCAGGGCGTGCCACAACTTGGAGAAGTTACCGGGGTCTATCGGCTCGTTGCTGAAGCTAGAGTACCTGGACTTGTCCGAATGCTTCTTACTTGAAGAAATGCCCAAAGAGATCGGCGAGCTGTCCAAGCTGCAGGTCCTCAAGGGTTTTCTCGTTGGCAGTTCAAGAAAGAAGAGCAGCCCATGCCGTCTCGCCGACCTGGCCACAAAGGCGCAGAATCTACGGAAGCTAAACATTACCACTGGACGGCAATCTTTGGTGTGTGATGAAGATGAGCTCTGCCAATTGGCGAAATGCCAGCATCTTGAGTCCTTGACAATAACTTGGATAGGAGAGGCATCTACCGAGCCATATCTTCCTCTTCCTTCCAGCCTCACAAAGCTTGATCTCCGGCGTGCCCCTACCGCAAGCCTTCTTAATATCATTCATCCATCGACGTCGGTGAGCCTGAAACGGCTGTATATTAGGGGAGGGAAGCTCCGTACACTGGGGCAAGACGGCGGCTGGAACGTTGAGACACTGCGTGCTAGATGTCTCAATGATTTGGAGTGTGAGTGGAGTGAGCTCCATGGTTTGTTCCGAGAACTCCGTTTTGTGGAGATGTGGAGGTGTGCCCGCCTTAGTTTCTGGCCTTGCGATGGGCGGGGCGTATGGGACAAAGGATCTCCTTCTCTGGCTCGCCGATCCATTGGAGTCTGCTACGGAGTGAAAGGCAACAATCTGCCACCATGGCATGAAGTCGTTCAGCTATATGCCTCGAACAACATTCCTGCCATGAGGATATTCTATCCTCACCACGATGTTCTTGAGGCTCTGAGAGGCACAGGCATCGGTATCAGCCTCGACGTTGAGGGCCAATTCCTCCCATCCTTCGCCTCTGAACCCTCCGTTGCGGCTGCCTGGGTGAAGACCAATGTGCAAGCCTTTTACCCTGCAGTCTCATTCAAGTTCATCACAGTCGGCAACCAGGTTGCCCTGAGAGAGATGAGATACATCCTCCCAGCGATGCAGAATATCTATGCAGCGTTGTCAGCAGTAGGGCTGGATCACATCAAGGTGTCAACGTCGGTGAGAAGGGACGTGCTGGGTTTGTCCTATCCTCCCTCTGCTGGTGCTTTCTCCTCAGCGATGGAACAGTACATGGCACCTATTGTGCAGTTCTTGGCCAAGATTGGGGCTCCACTTCTGGCCAGTGTGTTCCCTTACTTTACTTACGTGCACAATCAGGAAGGCATTGATATCGATTACGCCCTTTTCACCTCCCCGGGAACTGTGGTGCAGGACGGTGAACATAGCTATCAGAACCTCTTCGATGCCATTGTGGATGCTCTGTATTCGGCGATGGAGAAGGTGGGTGGCTCAACCGTCAGAATAGTTGTCTCAGATAGCGGCTGGCCATCGGCTGGGGCTCCTGCAGCGACCAAAGATAATGCTAGGGCCTACGTCCAGAACCTGATTAACCATGTGAGCAAAGGGACACCCAAGAGGCCCGTACCTATAGAGACATACATTTTTGCCATGTTCAATGAGAACGAGAAAACCGGTGACGAGATAGAGAGGAACTTTGGGCTCTTTGAACCTGACAAGAGCCCTGTGTACCCAATCACCTTCTCTTAA
Protein Sequence
  • >LOC_Os01g71930.1
    MAKATSTNNAFLSVFNLDMEYVKIHIAKSRITRVVQLGRWKRSRKHHIELVGDNDLLKKVLACKNLRYLSLRGISLIESIPEAIGTLAELLVLDLRACHNLEKLPGSIGSLLKLEYLDLSECFLLEEMPKEIGELSKLQVLKGFLVGSSRKKSSPCRLADLATKAQNLRKLNITTGRQSLVCDEDELCQLAKCQHLESLTITWIGEASTEPYLPLPSSLTKLDLRRAPTASLLNIIHPSTSVSLKRLYIRGGKLRTLGQDGGWNVETLRARCLNDLECEWSELHGLFRELRFVEMWRCARLSFWPCDGRGVWDKGSPSLARRSIGVCYGVKGNNLPPWHEVVQLYASNNIPAMRIFYPHHDVLEALRGTGIGISLDVEGQFLPSFASEPSVAAAWVKTNVQAFYPAVSFKFITVGNQVALREMRYILPAMQNIYAALSAVGLDHIKVSTSVRRDVLGLSYPPSAGAFSSAMEQYMAPIVQFLAKIGAPLLASVFPYFTYVHNQEGIDIDYALFTSPGTVVQDGEHSYQNLFDAIVDALYSAMEKVGGSTVRIVVSDSGWPSAGAPAATKDNARAYVQNLINHVSKGTPKRPVPIETYIFAMFNENEKTGDEIERNFGLFEPDKSPVYPITFS*