Gene Details:

Functional Descriptions:

  • OsCKX4 is predominantly expressed in leaf blades and roots.
  • Overexpression and RNA interference of OsCKX4 confirmed that OsCKX4 plays a positive role in crown root formation.
  • Moreover, expression analysis revealed a significant alteration in the expression of auxin-related genes in the ren1-D mutants, indicating that the OsCKX4 mediates crown root development by integrating the interaction between cytokinin and auxin.
  • Transgenic plants harboring OsCKX4 under the control of the root-specific promoter RCc3 displayed enhanced root development without affecting their shoot parts, suggesting that this strategy could be a powerful tool in rice root engineering.
  • OsCKX4 is induced by exogenous auxin and cytokinin in the roots.
  • Furthermore, one-hybrid assays revealed that OsCKX4 is a direct binding target of both the auxin response factor OsARF25 and the cytokinin response regulators OsRR2 and OsRR3.
  • Molecular and genetic analyses revealed that these phenotypes are caused by the activation of a cytokinin oxidase/dehydrogenase (CKX) family gene, OsCKX4.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g71310.1
    CCTCCCCTTACCTACCTTGGTGGCCTCCTCTCGCTCTCGCTCTCCTTCTCTTCCTGCACCTGTTGCACCCCCAACAACTCTCACTCACTGCCAGTGTCAAGCAAGATTATACAGCTTGGTCTCATCTCTTAATTCCAAGCTTGTTTCACCGACCTGCCAAACTCGATTGATCCACAGATAGATAGTACTTCTGGTTGGGATCTTGAGCTTATATAGACCAGGAAGCATGCGGGGAGCCATGAAGCCGTCGATCGTGCACTGCCTCAAGCTGCTCATGCTGCTGGCGCTCGGCGGGGTCACCATGCACGTCCCCGACGAGGACGACGTGGTCGCGTCGCTCGGGGCGCTGCGCCTCGACGGCCATTTCAGCTTCGACGACGCCCACGCCGCCGCCCGGGACTTCGGCAACCGGTGCAGCCTCCTGCCGGCGGCCGTGCTCCACCCTGGCTCGGTGTCCGACGTCGCCGCCACCGTCAGGCGCGTGTTCCAGCTGGGCAGGAGCTCGCCGCTCACCGTCGCGGCGCGCGGGCACGGCCACTCGCTCCTCGGCCAGTCCCAGGCCGCCGGCGGGATCGTCGTCAAGATGGAGTCCCTCGCCGCCGCCGCAGCCAGGGCGGTGCGGGTGCACGGCGGCGCGTCGCCCCACGTGGACGCCCCGGGCGGCGAGCTCTGGATCAACGTGCTGCATGAGACGCTCAAGCACGGGCTGGCGCCCAGGTCATGGACCGACTACCTCCATCTCACAGTCGGTGGCACCTTGTCGAATGCGGGGGTCAGCGGGCAGGCGTTCCGGCATGGACCGCAGGTCAGCAATGTCAACCAACTGGAGATTGTGACAGGGAGGGGAGAAGTTGTCACCTGCTCGCACGAGGTGAACTCTGATCTCTTCTACGCTGCTCTTGGCGGCCTGGGCCAGTTTGGGATCATCACCAGGGCTCGGATTGCTCTTGAACCTGCTCCAAAGATGGTGCGGTGGATACGTGTTCTCTACTCGGACTTTGAGACCTTCACCGAGGACCAGGAGAAGCTGATCGCGTCTGAGAAGACCTTCGACTACATCGAAGGGTTTGTGATCATAAACAGGACAGGCATCCTCAACAACTGGAGGACGTCGTTCAAGCCACAGGACCCAGTGCAGGCAAGCCAGTTCCAGTCGGATGGAAGAGTGCTATACTGCCTTGAGCTGACGATGAACTTCAACCACGATGAGGCTGACATCATGGAACAGGAAGTTGGTGCGCTGCTATCTCGACTCAGATACATATCGTCCACTCTATTCTACACCGATGTCACATACCTGGAGTTCTTGGACAGGGTGCACACTTCTGAGCTGAAGCTGAGGGCTCAAGGCCTCTGGGAAGTCCCACACCCGTGGCTGAATCTTCTGATCCCAAGGAGCACAGTCCACAAATTTGCAAAGGAAGTCTTCGGCAAGATCCTAAAAGATAGCAACAATGGTCCCATACTGCTTTACCCAGTGAACAGAACCAAGTGGGACAACAGAACATCAGTGGTCATACCAGATGAAGAAATTTTCTACCTGGTTGGGTTCCTATCTTCAGCACCATCATCCTCAGGTCATGGTAGTGTCGAACATGCAATGAACCTGAACAACAAAATAGTGGACTTCTGTGAAAAGAATGGTGTTGGGATGAAACAGTATCTAGCACCCTACACTACACAGAAGCAGTGGAAAGCCCACTTCGGAGCAAGGTGGGAGACATTTGAACGGAGGAAACACACGTACGATCCCCTAGCAATCCTAGCTCCAGGGCAGAGAATATTTCCAAAGGCATCACTACCTATGTCCTTGTGACAGTATATGCTGTGAAAAGGCTTTTGTAGAGCATATGTTGTACAAAAATGTAGCGAAAATATCCTTTGTAAAGACGAAATCTACGGAAGGTAGCTTAAAGAACACAGCGGGGCAACCAGTAGACTAGAACTCAAGAAGTAGTGGAAGATACCTCACAAAAGACTTCAAAGCAAAGCAGCGTGGCCTTAACTCTGGTTGCAGCTTTGGAGGCAAATGATTGGAGGATTACAGTGGTCCTAGCAACTGAAGTCCAGCTGTTGTTACAGCATAGAGAAGAGCTGTGCTACCTCAATGATCACTAAATCACTGACTAGTGTAACTCATGTTAAGTTGTTTGTGTTCAGCTAACAACACTGCAGAAGGTAGCATCAGGGTAGACCCTTGATTAGCAAATAACACAGCCTGTTGCATGTGAGTACTATCTGCATAAATGCAGCAGATTACATTAGTTTTTATGAATCGAATGTTATGTTTGCAAATGTTAAAGAAAAAAACAGCAAAGTTTCTTATTAATCATTCGTCTCCAAGAATAAATGGGACTGAATCATTTTATAATTCCCTTGATACGATAA
CDS Sequence
  • >LOC_Os01g71310.1
    ATGCGGGGAGCCATGAAGCCGTCGATCGTGCACTGCCTCAAGCTGCTCATGCTGCTGGCGCTCGGCGGGGTCACCATGCACGTCCCCGACGAGGACGACGTGGTCGCGTCGCTCGGGGCGCTGCGCCTCGACGGCCATTTCAGCTTCGACGACGCCCACGCCGCCGCCCGGGACTTCGGCAACCGGTGCAGCCTCCTGCCGGCGGCCGTGCTCCACCCTGGCTCGGTGTCCGACGTCGCCGCCACCGTCAGGCGCGTGTTCCAGCTGGGCAGGAGCTCGCCGCTCACCGTCGCGGCGCGCGGGCACGGCCACTCGCTCCTCGGCCAGTCCCAGGCCGCCGGCGGGATCGTCGTCAAGATGGAGTCCCTCGCCGCCGCCGCAGCCAGGGCGGTGCGGGTGCACGGCGGCGCGTCGCCCCACGTGGACGCCCCGGGCGGCGAGCTCTGGATCAACGTGCTGCATGAGACGCTCAAGCACGGGCTGGCGCCCAGGTCATGGACCGACTACCTCCATCTCACAGTCGGTGGCACCTTGTCGAATGCGGGGGTCAGCGGGCAGGCGTTCCGGCATGGACCGCAGGTCAGCAATGTCAACCAACTGGAGATTGTGACAGGGAGGGGAGAAGTTGTCACCTGCTCGCACGAGGTGAACTCTGATCTCTTCTACGCTGCTCTTGGCGGCCTGGGCCAGTTTGGGATCATCACCAGGGCTCGGATTGCTCTTGAACCTGCTCCAAAGATGGTGCGGTGGATACGTGTTCTCTACTCGGACTTTGAGACCTTCACCGAGGACCAGGAGAAGCTGATCGCGTCTGAGAAGACCTTCGACTACATCGAAGGGTTTGTGATCATAAACAGGACAGGCATCCTCAACAACTGGAGGACGTCGTTCAAGCCACAGGACCCAGTGCAGGCAAGCCAGTTCCAGTCGGATGGAAGAGTGCTATACTGCCTTGAGCTGACGATGAACTTCAACCACGATGAGGCTGACATCATGGAACAGGAAGTTGGTGCGCTGCTATCTCGACTCAGATACATATCGTCCACTCTATTCTACACCGATGTCACATACCTGGAGTTCTTGGACAGGGTGCACACTTCTGAGCTGAAGCTGAGGGCTCAAGGCCTCTGGGAAGTCCCACACCCGTGGCTGAATCTTCTGATCCCAAGGAGCACAGTCCACAAATTTGCAAAGGAAGTCTTCGGCAAGATCCTAAAAGATAGCAACAATGGTCCCATACTGCTTTACCCAGTGAACAGAACCAAGTGGGACAACAGAACATCAGTGGTCATACCAGATGAAGAAATTTTCTACCTGGTTGGGTTCCTATCTTCAGCACCATCATCCTCAGGTCATGGTAGTGTCGAACATGCAATGAACCTGAACAACAAAATAGTGGACTTCTGTGAAAAGAATGGTGTTGGGATGAAACAGTATCTAGCACCCTACACTACACAGAAGCAGTGGAAAGCCCACTTCGGAGCAAGGTGGGAGACATTTGAACGGAGGAAACACACGTACGATCCCCTAGCAATCCTAGCTCCAGGGCAGAGAATATTTCCAAAGGCATCACTACCTATGTCCTTGTGA
Protein Sequence
  • >LOC_Os01g71310.1
    MRGAMKPSIVHCLKLLMLLALGGVTMHVPDEDDVVASLGALRLDGHFSFDDAHAAARDFGNRCSLLPAAVLHPGSVSDVAATVRRVFQLGRSSPLTVAARGHGHSLLGQSQAAGGIVVKMESLAAAAARAVRVHGGASPHVDAPGGELWINVLHETLKHGLAPRSWTDYLHLTVGGTLSNAGVSGQAFRHGPQVSNVNQLEIVTGRGEVVTCSHEVNSDLFYAALGGLGQFGIITRARIALEPAPKMVRWIRVLYSDFETFTEDQEKLIASEKTFDYIEGFVIINRTGILNNWRTSFKPQDPVQASQFQSDGRVLYCLELTMNFNHDEADIMEQEVGALLSRLRYISSTLFYTDVTYLEFLDRVHTSELKLRAQGLWEVPHPWLNLLIPRSTVHKFAKEVFGKILKDSNNGPILLYPVNRTKWDNRTSVVIPDEEIFYLVGFLSSAPSSSGHGSVEHAMNLNNKIVDFCEKNGVGMKQYLAPYTTQKQWKAHFGARWETFERRKHTYDPLAILAPGQRIFPKASLPMSL*