Gene Details:

Functional Descriptions:

  • These data suggest that OsCLP, which has chitinase activity, may play an important role in plant defenses against pathogens.
  • In addition, exogenous treatment with OsCLP affected the growth of the basidiomycete fungus Rhizoctonia solani through degradation of the hyphal cell wall.
  • A T-DNA insertion mutant of OsCLP (OsCLP) showed significant retardation of root and shoot growth.
  • A comparative proteomic analysis was carried out using root tissue of wild-type and the OsCLP mutant to understand the OsCLP-mediated rice growth retardation.
  • Exogenous application of Ca(2+) resulted in successful recovery of both primary and lateral root growth in OsCLP.
  • Moreover, overexpression of OsCLP resulted in improved growth with modified seed shape and starch structure; however, the overall yield remained unaffected.
  • Taken together, our results highlight the involvement of OsCLP in rice growth by regulating the intracellular calcium concentrations.
  • Results obtained revealed that proteins related to glycolysis (phosphoglycerate kinase), stress adaption (chaperonin) and calcium signaling (calreticulin and CDPK1) were differentially regulated in OsCLP roots.
  • Fura-2 molecular probe staining, which is an intracellular calcium indicator, and ICP-MS analysis suggested that the intracellular calcium content was significantly lower in roots of OsCLP as compared to the wild-type.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os01g71080.1
    CACAGTGCGTGCTAGCTAGTTTGCCCAGGCTAATAACCAGCAATGTCGCTCCACCTCCTCCTCGCCGTCTCGCTCTGCGTCGCCTTGGCCTCGTCGTTGCCATGGGCAGCAGCTAGTGCCAATGGCAATGGCAATGGCAAGCCGCTTGTCGCGGCCATCACCAAGGACGCGGCGACCTCGCTCTACACCGTCCCCATCAAGGACGGCCGCCCGCTCGTCCTCGACCTCGCCGGCGCGCTCGTCTGGATGTCGTGCGCCGCCGCGCACCCGACGCTGGAGTGCCACCACCACTTCTGCATGCACGCGCACAGCTACCACCCGCCGGGATGCCCCCACAATGGCTATGGCCGCGCCGACGTCGAGGACCCGTTCCGGTGCAAGTGCACCGCCCACCCGTACAACCCCTTCTCCGGCGAGTCGGCGACGGCCGACCTGACGCGGACGAGGCTGTCGGCGAACGCCACCGACGGCAAGAACCCGCTCTACCCGGTGTCGTTCGCCGCCGTCACCTCCTGCGCGCCGGATTCCCTCCTCGCGAAGCTCCCCGCCGGCGCCGTCGGCGTCGCCGGGCTCGCGCGCACCAGGCTCGCGCTGCAGGCGCAGGTGGCGCGGTCGCAGAAGGTCGCCAACAAGTTCGCGCTCTGCCTCCCCAGCGGCGGCGGCGGCGACGGCGTGGCGATCTTCGGCGGCGGCCCGCTCTTCCTCCTCCCGCCGGGGCGTCCCGACGTGGCGGCGACGCTCGCCGGCGAAACTCCCCTCCACCGCAACAAGGATCTCCCCGGATACTTCATCTCGGCGACCAAGATCGCCGTGAACCAGGAGCAGGTGCAGCTCTACACCCAAGAGCCGCTCGTCGTCGAGCTGTGCACGAGGATCCCGTACACGGCGCTCCGGCCGGACGTGTACCGAGCGGTGGTCGACGCGTTCGCCAGGGCCACCGCCGGCCGGAAGCGCGTCACGCCGCCGCCGCCGCCGGCGGCGCCGTTCGAGCTGTGCTACGACTCGCGCGATCTGGGGTCGACGCGGCTGGGCTACGCCGTGCCGCAGATCGACCTGGTGCTGGAGGGCGGCAAGAACTGGACGGTGTTCGGCGGCAACTCGATGGCGCAGGTGAGCGACAACACGGCGTGCCTCGCGGTGGTGAAGGTGAAGGGGGAGAAGGGCAGCCCGCCGCCGCCGGCGGCGATCATCGGAGGGTTCCAGATGGAGAACAACCTGGTGGTGTTCGACGAGGAGAAGCAGCGGCTCGGATTCAGCGGCCTGCTCTGGGGGAGGCAGACCACCTGCAGCAACTTCAACTTCACTCTCGCCGCCTAGATCGAGTCGATTCCTCGAGCTCATAGCTTGCATTTGCATCGGAGATGTGCATGCATTTTTTTTTGGCATTATTATTATTATGAGTTGTGATTTGTAACAAGTACATTTCGATGAATAATGGAGAATACCACAGATTTGTTACTGAGAGCCTGAAATCGAAGAGGAATACAGGAAAAAAAAT
CDS Sequence
  • >LOC_Os01g71080.1
    ATGTCGCTCCACCTCCTCCTCGCCGTCTCGCTCTGCGTCGCCTTGGCCTCGTCGTTGCCATGGGCAGCAGCTAGTGCCAATGGCAATGGCAATGGCAAGCCGCTTGTCGCGGCCATCACCAAGGACGCGGCGACCTCGCTCTACACCGTCCCCATCAAGGACGGCCGCCCGCTCGTCCTCGACCTCGCCGGCGCGCTCGTCTGGATGTCGTGCGCCGCCGCGCACCCGACGCTGGAGTGCCACCACCACTTCTGCATGCACGCGCACAGCTACCACCCGCCGGGATGCCCCCACAATGGCTATGGCCGCGCCGACGTCGAGGACCCGTTCCGGTGCAAGTGCACCGCCCACCCGTACAACCCCTTCTCCGGCGAGTCGGCGACGGCCGACCTGACGCGGACGAGGCTGTCGGCGAACGCCACCGACGGCAAGAACCCGCTCTACCCGGTGTCGTTCGCCGCCGTCACCTCCTGCGCGCCGGATTCCCTCCTCGCGAAGCTCCCCGCCGGCGCCGTCGGCGTCGCCGGGCTCGCGCGCACCAGGCTCGCGCTGCAGGCGCAGGTGGCGCGGTCGCAGAAGGTCGCCAACAAGTTCGCGCTCTGCCTCCCCAGCGGCGGCGGCGGCGACGGCGTGGCGATCTTCGGCGGCGGCCCGCTCTTCCTCCTCCCGCCGGGGCGTCCCGACGTGGCGGCGACGCTCGCCGGCGAAACTCCCCTCCACCGCAACAAGGATCTCCCCGGATACTTCATCTCGGCGACCAAGATCGCCGTGAACCAGGAGCAGGTGCAGCTCTACACCCAAGAGCCGCTCGTCGTCGAGCTGTGCACGAGGATCCCGTACACGGCGCTCCGGCCGGACGTGTACCGAGCGGTGGTCGACGCGTTCGCCAGGGCCACCGCCGGCCGGAAGCGCGTCACGCCGCCGCCGCCGCCGGCGGCGCCGTTCGAGCTGTGCTACGACTCGCGCGATCTGGGGTCGACGCGGCTGGGCTACGCCGTGCCGCAGATCGACCTGGTGCTGGAGGGCGGCAAGAACTGGACGGTGTTCGGCGGCAACTCGATGGCGCAGGTGAGCGACAACACGGCGTGCCTCGCGGTGGTGAAGGTGAAGGGGGAGAAGGGCAGCCCGCCGCCGCCGGCGGCGATCATCGGAGGGTTCCAGATGGAGAACAACCTGGTGGTGTTCGACGAGGAGAAGCAGCGGCTCGGATTCAGCGGCCTGCTCTGGGGGAGGCAGACCACCTGCAGCAACTTCAACTTCACTCTCGCCGCCTAG
Protein Sequence
  • >LOC_Os01g71080.1
    MSLHLLLAVSLCVALASSLPWAAASANGNGNGKPLVAAITKDAATSLYTVPIKDGRPLVLDLAGALVWMSCAAAHPTLECHHHFCMHAHSYHPPGCPHNGYGRADVEDPFRCKCTAHPYNPFSGESATADLTRTRLSANATDGKNPLYPVSFAAVTSCAPDSLLAKLPAGAVGVAGLARTRLALQAQVARSQKVANKFALCLPSGGGGDGVAIFGGGPLFLLPPGRPDVAATLAGETPLHRNKDLPGYFISATKIAVNQEQVQLYTQEPLVVELCTRIPYTALRPDVYRAVVDAFARATAGRKRVTPPPPPAAPFELCYDSRDLGSTRLGYAVPQIDLVLEGGKNWTVFGGNSMAQVSDNTACLAVVKVKGEKGSPPPPAAIIGGFQMENNLVVFDEEKQRLGFSGLLWGRQTTCSNFNFTLAA*