Information report for REP-1;OsEP3A
Gene Details
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Functional Descriptions
- In rice (Oryza sativa), REP-1 is a primary cysteine proteinase responsible for the digestion of seed storage proteins to provide nutrients to support the growth of young seedlings.
- The expression of the gene for REP-1 (Rep1) is induced by gibberellins (GAs) and repressed by abscisic acid (ABA).
- An OsEP3A-specific DNA probe was used to study the effect of various factors on the expression of OsEP3A in germinating seeds and vegetative tissues of rice.
- The expression of OsEP3A is hormonally regulated in germinating seeds, spatially and temporally regulated in vegetative tissues, and nitrogen-regulated in suspension-cultured cells.
- Histochemical studies also indicate that the OsEP3A promoter is sufficient to confer the hormonal regulation of GUS expression in germinating seeds.
- In germinating rice seeds, a cysteine proteinase (REP-1), synthesized in aleurone-layer cells, is a key enzyme in the degradation of the major storage protein, glutelin.
- The OsEP3A promoter is sufficient to confer nitrogen regulation of GUS expression in suspension-cultured cells.
- Down-regulation of OsSAG12-1 results in enhanced senescence and pathogen-induced cell death in transgenic rice plants.
- Expression of OsSAG12-1 is induced during senescence and pathogen-induced cell death.
- Our results suggest that OsSAG12-1 is a negative regulator of cell death in rice.
Functional Keywords
- seedling , gibberellin , seed , growth , vegetative , nitrogen , senescence , cell-death
Literature and News
- Differential proteomic analysis of soluble extracellular proteins reveals the cysteine protease and cystatin involved in suspension-cultured cell proliferation in rice . DOI: 10.1016/j.bbapap.2008.11.023 ; PMID: 19118651
- Two cis-acting elements necessary and sufficient for gibberellin-upregulated proteinase expression in rice seeds . DOI: 10.1046/j.1365-313x.2003.01753.x ; PMID: 12787245
- Multiple mode regulation of a cysteine proteinase gene expression in rice . DOI: 10.1104/pp.122.1.57 ; PMID: 10631249
- Down-regulation of OsSAG12-1 results in enhanced senescence and pathogen-induced cell death in transgenic rice plants . DOI: 10.1007/s12038-013-9334-7 ; PMID: 23938390
Gene Resources
- UniProt: Q7F3A8
- EMBL: AB004819, AF099203, AP003380
- AlphaFoldDB: Q7F3A8
- EnsemblPlants: Os01t0907600-01
- Gramene: Os01t0907600-01
- KEGG: dosa:Os01g0907600
- Orthologous matrix: EKGNHWI
- InterPro: IPR000169, IPR000668, IPR013128
Sequences
cDNA Sequence
- >LOC_Os01g67980.1
ACGTCTCCTCCCTTCTCCTTCCTCCTCATCGCCCTCACCCTCCAATTCCAATCCCTCCCAAGAACTAGCTGATCACGACGAGCAGCTAGTCCACTGCTACTGCGATCGATCGGTTGCAAAGTTAATTAATTAGTGGCGATCGATCGATCATGGGGAGGGTTATTAGCAGCTGGAGGGTTCTTGCGGTGGTGGCTGCTTTGATGGCCATGGCGGCGGTGGAGCTGTGCGCCGCGATACCGTTCGACGAGAGGGATTTGGAGTCCGACGAGGCGCTGTGGGATCTGTACGAGCGGTGGCAGGAGCACCACCACGTGCCGCGCCACCACGGCGAGAAGCACCGCCGGTTCGGCGCGTTCAAGGACAACGTGAGGTACATCCACGAGCACAACAAGCGCGGCGGCCGGGGCTACCGCCTCCGGCTCAACCGCTTCGGCGACATGGGCAGAGAGGAGTTCCGCGCCACGTTCGCCGGGTCCCACGCCAACGACCTCCGCCGCGACGGCCTCGCCGCGCCGCCGCTCCCGGGGTTCATGTACGAGGGCGTCCGCGACCTCCCCCGCGCCGTCGACTGGCGCCGCAAGGGCGCGGTCACCGGCGTCAAGGACCAGGGCAAGTGCGGCAGCTGCTGGGCGTTCTCCACGGTGGTGTCCGTGGAGGGCATCAACGCGATCCGGACGGGGCGGCTGGTGTCGCTGTCGGAGCAGGAGCTGATCGACTGCGACACGGCGGACAACAGCGGCTGCCAGGGCGGGCTCATGGAGAACGCGTTCGAGTACATCAAGCACAGCGGCGGCATCACCACCGAGTCCGCCTACCCGTACCGCGCCGCCAACGGAACGTGCGACGCCGTCCGCGCGCGGCGCGCGCCGCTGGTGGTGATCGACGGGCACCAGAACGTGCCGGCCAACAGCGAGGCCGCGCTCGCCAAGGCCGTCGCCAACCAGCCCGTCTCCGTCGCCATCGACGCCGGCGACCAGTCCTTCCAGTTCTACTCCGACGGCGTCTTCGCCGGCGACTGCGGCACCGACCTCGACCACGGCGTCGCGGTGGTCGGCTACGGCGAGACCAACGACGGCACGGAGTACTGGATCGTCAAGAACTCGTGGGGCACAGCCTGGGGCGAGGGCGGCTACATCCGGATGCAGCGCGACTCCGGCTACGACGGCGGCCTCTGCGGCATCGCCATGGAAGCCTCCTACCCCGTCAAGTTCTCGCCAAATCGTGTCACGCCAAGGCGCGCCCTTGGCGCCAAGGAAACCCAGTGATCGAGCCGGCGCCATCTCCGCCGTACACGTGCCCGCCTACGAGCCTGGATCAACGGCTCTGGTCGCGTCTCCTCCTCCAGCTTGCAAAATGGTGCTATTATTGTTGCTGCTCTTTGTTAATTGCCTTGTGTTTATGTAATTCGTGTGAGGCGATCAGGGAAAAGAATTACCTGAAATCGTCTAAGTTACTAGTGTTATTTCGTGTGTGCTAAATAAGTTTTAGATGTGAGGTGAGGTCGATCTATCATGTAATGTACTGAACTTATATGTGGTGTTCTTCGTGGCACCGGGAAATATATATAAGAAGGTTGTTGTTATGACTAAGGTTATGCCAAATTTCATCCAAAAAAGACTAAGGTTATGCCAAATTTCATCCAAAAAAAA
CDS Sequence
- >LOC_Os01g67980.1
ATGGGGAGGGTTATTAGCAGCTGGAGGGTTCTTGCGGTGGTGGCTGCTTTGATGGCCATGGCGGCGGTGGAGCTGTGCGCCGCGATACCGTTCGACGAGAGGGATTTGGAGTCCGACGAGGCGCTGTGGGATCTGTACGAGCGGTGGCAGGAGCACCACCACGTGCCGCGCCACCACGGCGAGAAGCACCGCCGGTTCGGCGCGTTCAAGGACAACGTGAGGTACATCCACGAGCACAACAAGCGCGGCGGCCGGGGCTACCGCCTCCGGCTCAACCGCTTCGGCGACATGGGCAGAGAGGAGTTCCGCGCCACGTTCGCCGGGTCCCACGCCAACGACCTCCGCCGCGACGGCCTCGCCGCGCCGCCGCTCCCGGGGTTCATGTACGAGGGCGTCCGCGACCTCCCCCGCGCCGTCGACTGGCGCCGCAAGGGCGCGGTCACCGGCGTCAAGGACCAGGGCAAGTGCGGCAGCTGCTGGGCGTTCTCCACGGTGGTGTCCGTGGAGGGCATCAACGCGATCCGGACGGGGCGGCTGGTGTCGCTGTCGGAGCAGGAGCTGATCGACTGCGACACGGCGGACAACAGCGGCTGCCAGGGCGGGCTCATGGAGAACGCGTTCGAGTACATCAAGCACAGCGGCGGCATCACCACCGAGTCCGCCTACCCGTACCGCGCCGCCAACGGAACGTGCGACGCCGTCCGCGCGCGGCGCGCGCCGCTGGTGGTGATCGACGGGCACCAGAACGTGCCGGCCAACAGCGAGGCCGCGCTCGCCAAGGCCGTCGCCAACCAGCCCGTCTCCGTCGCCATCGACGCCGGCGACCAGTCCTTCCAGTTCTACTCCGACGGCGTCTTCGCCGGCGACTGCGGCACCGACCTCGACCACGGCGTCGCGGTGGTCGGCTACGGCGAGACCAACGACGGCACGGAGTACTGGATCGTCAAGAACTCGTGGGGCACAGCCTGGGGCGAGGGCGGCTACATCCGGATGCAGCGCGACTCCGGCTACGACGGCGGCCTCTGCGGCATCGCCATGGAAGCCTCCTACCCCGTCAAGTTCTCGCCAAATCGTGTCACGCCAAGGCGCGCCCTTGGCGCCAAGGAAACCCAGTGA
Protein Sequence
- >LOC_Os01g67980.1
MGRVISSWRVLAVVAALMAMAAVELCAAIPFDERDLESDEALWDLYERWQEHHHVPRHHGEKHRRFGAFKDNVRYIHEHNKRGGRGYRLRLNRFGDMGREEFRATFAGSHANDLRRDGLAAPPLPGFMYEGVRDLPRAVDWRRKGAVTGVKDQGKCGSCWAFSTVVSVEGINAIRTGRLVSLSEQELIDCDTADNSGCQGGLMENAFEYIKHSGGITTESAYPYRAANGTCDAVRARRAPLVVIDGHQNVPANSEAALAKAVANQPVSVAIDAGDQSFQFYSDGVFAGDCGTDLDHGVAVVGYGETNDGTEYWIVKNSWGTAWGEGGYIRMQRDSGYDGGLCGIAMEASYPVKFSPNRVTPRRALGAKETQ*