CDKB1;1;CDKB1

Gene Details:

In plants, the CDKB1;1 transcripts were highly expressed in the shoot and root apical meristems, but not in mature plant organs.
In Arabidopsis, four A2-type cyclins (CYCA2s) function redundantly in regulating CDKB1 activity to promote the asymmetric division for stomatal initiation and the symmetric division of guard mother cells (GMCs).
Cross-species complementation tests demonstrated that OsCYCA2;1 and OsCDKB1;1 could complement the defective stomatal phenotypes of Arabidopsis cyca2 and cdkb1 mutants, but also could suppress DNA endoduplication and cell enlargement.
Additionally, Yeast one-hybrid assay and electrophoretic mobility shift assay (EMSA) showed that OsFLP directly bound to the promoter region of Oryza sativa B-type Cyclin-Dependent Kinase 1;1 (OsCDKB1;1), and the expression of OsCDKB1;1 was repressed in osflp.
Disturbing the expression of OsCDKB1;1 remarkably enhanced the tolerance to salt stress.
Taken together, our findings reveal a crucial function of OsFLP regulating OsCDKB1;1 in salt tolerance and largely extend the knowledge about the role of OsFLP in rice.

cDNA Sequence

>LOC_Os01g67160.1
ATGGAGAAGTACGAGAAGCTGGAGAAGGTGGGGGAAGGGACGTACGGGAAGGTGTACAAGGCGCAGGACAGGGCGACGGGGCAGCTGGTGGCGCTGAAGAAGACGAGGCTGGAGATGGACGAGGAGGGGATCCCGCCCACCGCGCTGCGCGAGATCTCCATCCTCAGGCTGCTCTCCCAGTCGCTCTACGTCGTCCGCCTCCTCTCCGTCGAGCAGGCCACCAAGAACGGCAAGCCCGTCCTCTACCTCGTCTTCGAGTTCCTCGACACCGACCTCAAGAAGTTCGTCGACGCCTACCGCAAGGGCCCCAACCCTCGCCCCCTCCCCACCAACGTCATCAAGAGCTTCTTGTATCAGTTATGCAAAGGAGTCGCACATTGCCATGGCCATGGTGTCCTTCACCGTGATTTAAAGCCACAAAACCTGTTGGTCGACAAGGAAAAGGGGATATTGAAAATTGCTGATCTTGGGCTAGGTAGGGCGTTCACTGTTCCTATGAAAAGCTACACACATGAGATTGTGACTCTTTGGTACAGAGCTCCTGAAGTTTTGCTTGGATCAACACATTACTCAACCGGTGTTGACATTTGGTCCGTTGGTTGCATCTTCGCTGAAATGGTCAGAAGACAAGCTCTTTTTCCAGGTGACTCTGAGTTGCAACAGTTGCTTCACATTTTCAGGTTGTTGGGAACTCCTACTGAGGAGCAGTGGCCTGGAGTAACTGATTTGAGGGACTGGCATGAGTTTCCACAGTGGAAGCCACAGATTTTAGAACGTCAAGTCCCATCATTGGAGCCTGAAGGAGTTGACCTTTTATCGAAAATGCTCCAGTATAACCCAGCAAATCGGATCTCAGCAAAGGCTGCTATGGAACACCCCTACTTCGACAGCCTCGACAAGTCCCAGTTCTAGTTTGTACCATGTGATTGACGAAGTCCATGTTCTGTTGTGTCATTTGATTATAAACGTGTTGCTGTTAGCAGCAAGGAAATTCGTTTTGCCTACTTAGCACCCTACTTTGTGATCCAACAAGGAAATCCGTTTGCCATCTAGGCAACTTGAATACAGTTTGAATGTAAAATGGGTTTGTTTCTATGCTGTATGGCATATGTATTATGGACAAGAAGGTTGCAATATATTTGCCTGTTTCTTTG

CDS Sequence

>LOC_Os01g67160.1
ATGGAGAAGTACGAGAAGCTGGAGAAGGTGGGGGAAGGGACGTACGGGAAGGTGTACAAGGCGCAGGACAGGGCGACGGGGCAGCTGGTGGCGCTGAAGAAGACGAGGCTGGAGATGGACGAGGAGGGGATCCCGCCCACCGCGCTGCGCGAGATCTCCATCCTCAGGCTGCTCTCCCAGTCGCTCTACGTCGTCCGCCTCCTCTCCGTCGAGCAGGCCACCAAGAACGGCAAGCCCGTCCTCTACCTCGTCTTCGAGTTCCTCGACACCGACCTCAAGAAGTTCGTCGACGCCTACCGCAAGGGCCCCAACCCTCGCCCCCTCCCCACCAACGTCATCAAGAGCTTCTTGTATCAGTTATGCAAAGGAGTCGCACATTGCCATGGCCATGGTGTCCTTCACCGTGATTTAAAGCCACAAAACCTGTTGGTCGACAAGGAAAAGGGGATATTGAAAATTGCTGATCTTGGGCTAGGTAGGGCGTTCACTGTTCCTATGAAAAGCTACACACATGAGATTGTGACTCTTTGGTACAGAGCTCCTGAAGTTTTGCTTGGATCAACACATTACTCAACCGGTGTTGACATTTGGTCCGTTGGTTGCATCTTCGCTGAAATGGTCAGAAGACAAGCTCTTTTTCCAGGTGACTCTGAGTTGCAACAGTTGCTTCACATTTTCAGGTTGTTGGGAACTCCTACTGAGGAGCAGTGGCCTGGAGTAACTGATTTGAGGGACTGGCATGAGTTTCCACAGTGGAAGCCACAGATTTTAGAACGTCAAGTCCCATCATTGGAGCCTGAAGGAGTTGACCTTTTATCGAAAATGCTCCAGTATAACCCAGCAAATCGGATCTCAGCAAAGGCTGCTATGGAACACCCCTACTTCGACAGCCTCGACAAGTCCCAGTTCTAG

Protein Sequence

>LOC_Os01g67160.1
MEKYEKLEKVGEGTYGKVYKAQDRATGQLVALKKTRLEMDEEGIPPTALREISILRLLSQSLYVVRLLSVEQATKNGKPVLYLVFEFLDTDLKKFVDAYRKGPNPRPLPTNVIKSFLYQLCKGVAHCHGHGVLHRDLKPQNLLVDKEKGILKIADLGLGRAFTVPMKSYTHEIVTLWYRAPEVLLGSTHYSTGVDIWSVGCIFAEMVRRQALFPGDSELQQLLHIFRLLGTPTEEQWPGVTDLRDWHEFPQWKPQILERQVPSLEPEGVDLLSKMLQYNPANRISAKAAMEHPYFDSLDKSQF*