Information report for OsPIE3;OsPUB16
Gene Details
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Functional Descriptions
- E3 ubiquitin ligase OsPIE3 destabilises the B-lectin receptor-like kinase PID2 to control blast disease resistance in rice.
- The U-box E3 ubiquitin ligase OsPIE3 (PID2-interacting E3) was isolated and confirmed to play key roles in PID2-mediated rice blast resistance.
- Genetic analysis suggested that OsPIE3 loss-of-function mutants exhibited enhanced resistance to M.
- oryzae isolate ZB15, whereas mutants with overexpressed OsPIE3 exhibited reduced resistance.
- Furthermore, the OsPIE3/PID2-double mutant displayed a similar blast phenotype to that of the PID2 single mutant, suggesting that OsPIE3 is a negative regulator and functions along with PID2 in blast disease resistance.
- Our findings confirm that the E3 ubiquitin ligase OsPIE3 is necessary for PID2-mediated rice blast disease resistance regulation.
- Further investigation demonstrated that OsPIE3 can modify the subcellular localisation of PID2, thus promoting its nuclear recruitment from the plasma membrane for protein degradation in the ubiquitin-proteasome system.
- Mechanistic investigations revealed that OsPUB16 mediated the ubiquitination and degradation of OsMADS23, which is the substrate of OSMOTIC STRESS/ABA-ACTIVATED PROTEIN KINASE 9 (SAPK9) and increases rice drought tolerance by promoting ABA biosynthesis.
- Loss-of-function mutants of OsPUB16 generated through CRISPR/Cas9 system exhibited the markedly enhanced water-deficit tolerance, while OsPUB16 overexpression lines were hypersensitive to water deficit stress.
- Rice OsPUB16 modulates the 'SAPK9-OsMADS23-OsAOC' pathway to reduce plant water-deficit tolerance by repressing ABA and JA biosynthesis.
- Moreover, OsPUB16 negatively regulated ABA and JA response, and OsPUB16 mutants produced more endogenous ABA and JA than wild type when exposed to water deficit.
- Collectively, our findings establish that OsPUB16 reduces plant water-deficit tolerance by modulating the 'SAPK9-OsMADS23-OsAOC' pathway to repress ABA and JA biosynthesis.
Functional Keywords
- Kinase , kinase , resistance , disease , disease-resistance , blast , R-protein , blast-resistance , plasma-membrane , Ubiquitin , blast-disease , drought , stress , ja , JA , tolerance , ABA , drought-tolerance , protein-kinase , ABA-biosynthesis , JA-biosynthesis , osmotic-stress
Literature and News
- E3 ubiquitin ligase OsPIE3 destabilises the B-lectin receptor-like kinase PID2 to control blast disease resistance in rice . DOI: 10.1111/nph.18637 ; PMID: 36440499
- Rice OsPUB16 modulates the ‘SAPK9-OsMADS23-OsAOC’ pathway to reduce plant water-deficit tolerance by repressing ABA and JA biosynthesis . DOI: 10.1371/journal.pgen.1010520 ; PMID: 36441771
Gene Resources
- UniProt: A0A9K3Y6Z2
- EMBL: AP003561, AP008207
- KEGG: dosa:Os01g0884400, osa:4325007
- InterPro: IPR000225, IPR003613, IPR011989
- PANTHER: PTHR23315, PTHR23315:SF237
- SUPFAM: SSF48371, SSF57850
- PROSITE: PS50176, PS51698
- Gene3D: 1.25.10.10, 3.30.40.10
- OrthoDB: A0A9K3Y6Z2
- SWISS-MODEL: A0A9K3Y6Z2
- Conserved Domain Database: cd16664
Sequences
cDNA Sequence
- >LOC_Os01g66130.1
GTGTTCACACGCTACCCCGACTCGTCGCCTTCGCCTTGCTCGTCTTCGTCTCCGCCTCCTGCGACGAAGCGAAGCGAGACGCCTCCGCCTCGCGCGAGCCACCACGAGCACCTCGACACGGGAGTCCGAATCCTCCTCCTCCCCCCTCCATTGGAGGCCTCCGAGGTGATGGATTCAGTGTCATTGTCACTACTCGATAGTATTTCAAATTTCCGGGTGCTGTCTTCAAGCAATGCCTCGAAAACAGAGCTAGTTAAGAAATATTGCCAAACGATGGATGGCATCCTTGATCACTTGGAGGTGGCCCTAAACAGAGCTTTTCCTCAGATTACTCCAGATGGTGAACTAAGTAAAGTGCTTGAAGAACTTGGCGCTACCATCAATGAAGCGACTGAGCTAGTTGGAGGCTGGAATCAAATGATGAGCAAGATTTATTTTGTTATTCAGGCTGATTCAATTATTGCCAAGATGCAGATATATGTATTCGAATTATGCCAAATTGTCAATTCTCTCATGCAGATTGAGTCAATGCATTTGGAGGATCTTGAACACGATAGCTGTGGAAAAATTTCAGATGTCATTAGGGAGGCTTCCAGGGCTTTAGCAGGGGAAGTTATGCCAAATTCAGAGGAATTTGGAAAGATTCAAACTACTTTGAGCTTATCCACAAATCAGGAGTTGCTGATGGAATATGTTGCACTTGTTAAGGTTAAAACAAAAGGTAATCATGAAGATAACAAAGAAATGGATGATATTAACGATATTGTTGAATTAGTCAACCATATGCTTGACAAACATGTGGAAGAAAAGCAAACACGTAGCATTAATGGAGTGACCATTCCTGCTGATTTTTGTTGTCCTCTTTCCCTTGAACTAATGTCGGATCCAGTGATTGTGGCATCTGGTCAAACGTATGAGCATGTTTTTATCAGAAAATGGTTTGATCTGGGATACAACATTTGTCCAAAGACACGCCAAATATTGGGACACACCAAATTGATTCCTAACTTCACTGTCAAACAGTTGATTGAAAATTGGTGTGAGGTACATGGTATAATGCTACCAGATCCTGTTAAACTCTTGAGTTTGTGCTTCCCTGTTTCCCTCAACATCACAGATGGAAGTGCAAGTGCAGACAAGTCTGGATCACCAGAACACTGCCAATTGGTAGCTGCATTGCATCCAAAAGCACAGTGCGCATCGGATGATAGTCATCATTATAATTTGATACATGAAAACTCTGATTCAGATGATAGAGTGTCATCATTTGGAGACACAGATGATTCTGAACCTGATTCTTTAAGATTATCAACAGAAACTACTGCAGCAAACAAATCTCTACTTGATGAAAAAACTGATCGTTCTGATGGTCTTAAGCAATTGAGAGACAATGGTTTTCAAGTTTCTGATGAGGAACAGTATCTCGAAAGGAATGGTAAAAGTCATATCAGCAGCCATCATCAACTTGAAGTTGATGGAGAGAATGTCAGGGTACAAGCATCAAGTGACATCAATGCATCTGAAGTTATGCAAGATGATCCGGTCACCACATGTTCAAAGGTATCAGATAACCCTCCTAGATTGGGTGGTGTTCGTTCTCGAAATCAGCCAAACTGGTGGAGACAGTCTAATAAAACTATTCCTAGGATCGGATTGTCATCTTCGACAGATTCAAAACCAGATTTTTCTGGCAATGATGCTAAAGTGCGTAATCTTATCGAGGAACTGAAAAGTGATTCTGCTGAGGTCCAAAGGTCAGCAACAGGAGAGCTCCGCATTCTTTCTAGACACAGCTTGGAGAATAGAATTGCCATCGCAAACTGCGGAGCAATCCCCTTCTTGGTGAGTCTACTTCATTCTACAGACCCCAGCACACAAGAAAATGCTGTGACAATTCTCCTGAATTTGTCATTGGATGACAATAACAAGATTGCCATAGCAAGTGCTGAGGCCATTGAGCCTCTCATCTTCGTTCTTCAGGTGGGAAACCCCGAAGCGAAAGCCAACTCAGCTGCAACTTTATTCAGCCTCTCAGTCATTGAAGAGAACAAGATCAAGATTGGACGTTCCGGTGCCATCGAACCATTAGTAGATTTACTGGGAGAAGGTACCCCGCAAGGGAAGAAGGATGCAGCTACTGCACTCTTCAATCTGTCGATATTTCATGAACACAAGACCCGCATTGTTCAGGCTGGGGCTGTCAACCACCTGGTGGAGCTGATGGATCCAGCTGCTGGGATGGTTGATAAAGCTGTTGCTGTTCTGGCAAACCTTGCGACTGTGCATGATGGAAGGAATGCCATTGCTCAGGCAGGAGGCATCCGAGTACTGGTTGAGGTTGTTGAGCTGGGTTCTGCACGTTCAAAGGAGAATGCCGCTGCTGCCCTGCTACAACTCTGCACAAACAGTAACAGGTTTTGCACCCTGGTTCTTCAAGAAGGCGTCGTGCCACCTTTGGTTGCATTGTCGCAATCAGGCACAGCCCGTGCAAGAGAGAAGGCTCAGGTTCTTCTAAGCTATTTTCGCAACCAGCGCCACGTCAGGGTTGGGAGAGGGTAAGTCTACGCAATTAAGCAGCAATGCGGCGGTCTGATTATTGTTCCATGATCCTTGTTGGCCGGGCAATTAAGAGATGTATATTCCATCCTAGTCGTAGCATATTTTTTCTGGTAGTGATTCCAAATGATTGATGTCACAAGTTGCTTCCTGTAAAGAAGACCCATCTATTGTGTATACATGGTTTTCACGATATTTAGTTCACTTCTGTACTGAAA
CDS Sequence
- >LOC_Os01g66130.1
ATGGATTCAGTGTCATTGTCACTACTCGATAGTATTTCAAATTTCCGGGTGCTGTCTTCAAGCAATGCCTCGAAAACAGAGCTAGTTAAGAAATATTGCCAAACGATGGATGGCATCCTTGATCACTTGGAGGTGGCCCTAAACAGAGCTTTTCCTCAGATTACTCCAGATGGTGAACTAAGTAAAGTGCTTGAAGAACTTGGCGCTACCATCAATGAAGCGACTGAGCTAGTTGGAGGCTGGAATCAAATGATGAGCAAGATTTATTTTGTTATTCAGGCTGATTCAATTATTGCCAAGATGCAGATATATGTATTCGAATTATGCCAAATTGTCAATTCTCTCATGCAGATTGAGTCAATGCATTTGGAGGATCTTGAACACGATAGCTGTGGAAAAATTTCAGATGTCATTAGGGAGGCTTCCAGGGCTTTAGCAGGGGAAGTTATGCCAAATTCAGAGGAATTTGGAAAGATTCAAACTACTTTGAGCTTATCCACAAATCAGGAGTTGCTGATGGAATATGTTGCACTTGTTAAGGTTAAAACAAAAGGTAATCATGAAGATAACAAAGAAATGGATGATATTAACGATATTGTTGAATTAGTCAACCATATGCTTGACAAACATGTGGAAGAAAAGCAAACACGTAGCATTAATGGAGTGACCATTCCTGCTGATTTTTGTTGTCCTCTTTCCCTTGAACTAATGTCGGATCCAGTGATTGTGGCATCTGGTCAAACGTATGAGCATGTTTTTATCAGAAAATGGTTTGATCTGGGATACAACATTTGTCCAAAGACACGCCAAATATTGGGACACACCAAATTGATTCCTAACTTCACTGTCAAACAGTTGATTGAAAATTGGTGTGAGGTACATGGTATAATGCTACCAGATCCTGTTAAACTCTTGAGTTTGTGCTTCCCTGTTTCCCTCAACATCACAGATGGAAGTGCAAGTGCAGACAAGTCTGGATCACCAGAACACTGCCAATTGGTAGCTGCATTGCATCCAAAAGCACAGTGCGCATCGGATGATAGTCATCATTATAATTTGATACATGAAAACTCTGATTCAGATGATAGAGTGTCATCATTTGGAGACACAGATGATTCTGAACCTGATTCTTTAAGATTATCAACAGAAACTACTGCAGCAAACAAATCTCTACTTGATGAAAAAACTGATCGTTCTGATGGTCTTAAGCAATTGAGAGACAATGGTTTTCAAGTTTCTGATGAGGAACAGTATCTCGAAAGGAATGGTAAAAGTCATATCAGCAGCCATCATCAACTTGAAGTTGATGGAGAGAATGTCAGGGTACAAGCATCAAGTGACATCAATGCATCTGAAGTTATGCAAGATGATCCGGTCACCACATGTTCAAAGGTATCAGATAACCCTCCTAGATTGGGTGGTGTTCGTTCTCGAAATCAGCCAAACTGGTGGAGACAGTCTAATAAAACTATTCCTAGGATCGGATTGTCATCTTCGACAGATTCAAAACCAGATTTTTCTGGCAATGATGCTAAAGTGCGTAATCTTATCGAGGAACTGAAAAGTGATTCTGCTGAGGTCCAAAGGTCAGCAACAGGAGAGCTCCGCATTCTTTCTAGACACAGCTTGGAGAATAGAATTGCCATCGCAAACTGCGGAGCAATCCCCTTCTTGGTGAGTCTACTTCATTCTACAGACCCCAGCACACAAGAAAATGCTGTGACAATTCTCCTGAATTTGTCATTGGATGACAATAACAAGATTGCCATAGCAAGTGCTGAGGCCATTGAGCCTCTCATCTTCGTTCTTCAGGTGGGAAACCCCGAAGCGAAAGCCAACTCAGCTGCAACTTTATTCAGCCTCTCAGTCATTGAAGAGAACAAGATCAAGATTGGACGTTCCGGTGCCATCGAACCATTAGTAGATTTACTGGGAGAAGGTACCCCGCAAGGGAAGAAGGATGCAGCTACTGCACTCTTCAATCTGTCGATATTTCATGAACACAAGACCCGCATTGTTCAGGCTGGGGCTGTCAACCACCTGGTGGAGCTGATGGATCCAGCTGCTGGGATGGTTGATAAAGCTGTTGCTGTTCTGGCAAACCTTGCGACTGTGCATGATGGAAGGAATGCCATTGCTCAGGCAGGAGGCATCCGAGTACTGGTTGAGGTTGTTGAGCTGGGTTCTGCACGTTCAAAGGAGAATGCCGCTGCTGCCCTGCTACAACTCTGCACAAACAGTAACAGGTTTTGCACCCTGGTTCTTCAAGAAGGCGTCGTGCCACCTTTGGTTGCATTGTCGCAATCAGGCACAGCCCGTGCAAGAGAGAAGGCTCAGGTTCTTCTAAGCTATTTTCGCAACCAGCGCCACGTCAGGGTTGGGAGAGGGTAA
Protein Sequence
- >LOC_Os01g66130.1
MDSVSLSLLDSISNFRVLSSSNASKTELVKKYCQTMDGILDHLEVALNRAFPQITPDGELSKVLEELGATINEATELVGGWNQMMSKIYFVIQADSIIAKMQIYVFELCQIVNSLMQIESMHLEDLEHDSCGKISDVIREASRALAGEVMPNSEEFGKIQTTLSLSTNQELLMEYVALVKVKTKGNHEDNKEMDDINDIVELVNHMLDKHVEEKQTRSINGVTIPADFCCPLSLELMSDPVIVASGQTYEHVFIRKWFDLGYNICPKTRQILGHTKLIPNFTVKQLIENWCEVHGIMLPDPVKLLSLCFPVSLNITDGSASADKSGSPEHCQLVAALHPKAQCASDDSHHYNLIHENSDSDDRVSSFGDTDDSEPDSLRLSTETTAANKSLLDEKTDRSDGLKQLRDNGFQVSDEEQYLERNGKSHISSHHQLEVDGENVRVQASSDINASEVMQDDPVTTCSKVSDNPPRLGGVRSRNQPNWWRQSNKTIPRIGLSSSTDSKPDFSGNDAKVRNLIEELKSDSAEVQRSATGELRILSRHSLENRIAIANCGAIPFLVSLLHSTDPSTQENAVTILLNLSLDDNNKIAIASAEAIEPLIFVLQVGNPEAKANSAATLFSLSVIEENKIKIGRSGAIEPLVDLLGEGTPQGKKDAATALFNLSIFHEHKTRIVQAGAVNHLVELMDPAAGMVDKAVAVLANLATVHDGRNAIAQAGGIRVLVEVVELGSARSKENAAAALLQLCTNSNRFCTLVLQEGVVPPLVALSQSGTARAREKAQVLLSYFRNQRHVRVGRG*