OsNAC6;SNAC2

Gene Details

MSU gene ID: LOC_Os01g66120
RAPdb gene ID: Os01g0884300
Gene Symbol: OsNAC6 SNAC2
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

Our results imply that OsNAC6, besides having a role in plant adaptation to abiotic stresses, also integrates signals derived from both abiotic and biotic stresses.
Transgenic rice plants over-expressing OsNAC6 constitutively exhibited growth retardation and low reproductive yields.
The root phenotype of OsNAC6 knock-out seedlings was observed to be similar to that of the OsHDAC1(OE) seedlings.
Conversely, the root phenotype of the OsNAC6 overexpressors was similar to that of the OsHDAC1 knock-out seedlings.
These observations indicate that OsHDAC1 negatively regulates the OsNAC6 gene that primarily mediates the alteration in the root growth of the OsHDAC1(OE) seedlings.
The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice.
OsNAC6 was also induced by jasmonic acid (JA), a plant hormone that activates defense responses against herbivores and pathogens.
Expression of OsNAC6 is induced by abiotic stresses, including cold, drought and high salinity.
The growth of these plants was similar to that of control plants, whereas the growth of OsNAC6-overexpressing transgenic plants was retarded.
Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different.
These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice.
Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice.
Northern blot and SNAC2 promoter activity analyses suggest that SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment.
Here we show that OsNAC6 is induced by cold, salt, drought and abscisic acid (ABA).
By utilizing stress-inducible promoters, such as the OsNAC6 promoter, it is hoped that stress-inducible over-expression of OsNAC6 in rice can improve stress tolerance by suppressing the negative effects of OsNAC6 on growth under normal growth conditions.
OsNAC6 gene expression is also induced by wounding and blast disease.
The OsNAC6 gene is a member of the NAC transcription factor gene family in rice.
Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice.
The results of microarray analysis revealed that many genes that are inducible by abiotic and biotic stresses were upregulated in rice plants over-expressing OsNAC6.
Collectively, these results indicate that OsNAC6 functions as a transcriptional activator in response to abiotic and biotic stresses in plants.
The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance.
Multiyear drought field tests demonstrated that the grain yield of OsNAC6 root-specific overexpressing transgenic rice lines was less affected by drought stress than were non-transgenic controls.
Collectively, OsNAC6 orchestrates novel molecular drought tolerance mechanisms and has potential for the biotechnological development of high-yielding crops under water limiting conditions.
Genome-wide analyses of loss- and gain-of-function mutants revealed that OsNAC6 up-regulates the expression of direct target genes involved in membrane modification, nicotianamine (NA) biosynthesis, glutathione relocation, 3’-phophoadenosine 5’-phosphosulfate accumulation, and glycosylation, which represent multiple drought tolerance pathways.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os01g66120.1
GTCGCTTGCCCCCGGCTCTATAAATGCCCGGCTCCTCACCCGGAACAAGTTTCAAGCCCTCCTCTCCTCTTCCCAACACTAGTAGGATAAAGCCACAGAGAGAGCAGTAGTAGTAGCGAGCTCGCCGGAGAACGGACGATCACCGGAGAAGGGGGAGAGAGATGAGCGGCGGTCAGGACCTGCAGCTGCCGCCGGGGTTCCGGTTCCACCCGACGGACGAGGAGCTGGTGATGCACTACCTCTGCCGCCGCTGCGCCGGCCTCCCCATCGCCGTCCCCATCATCGCCGAGATCGACCTCTACAAGTTCGATCCATGGCAGCTTCCCCGGATGGCGCTGTACGGAGAGAAGGAGTGGTACTTCTTCTCCCCGCGAGACCGCAAGTACCCGAACGGGTCGCGGCCGAACCGCGCCGCCGGGTCGGGGTACTGGAAGGCGACCGGCGCCGACAAGCCGGTGGGCTCGCCGAAGCCGGTGGCGATCAAGAAGGCCCTCGTCTTCTACGCCGGCAAGGCGCCCAAGGGCGAGAAGACCAACTGGATCATGCACGAGTACCGCCTCGCCGACGTCGACCGCTCCGCCCGCAAGAAGAACAGCCTCAGGTTGGATGATTGGGTGCTGTGCCGGATTTACAACAAGAAGGGCGGGCTGGAGAAGCCGCCGGCCGCGGCGGTGGCGGCGGCGGGGATGGTGAGCAGCGGCGGCGGCGTCCAGAGGAAGCCGATGGTGGGGGTGAACGCGGCGGTGAGCTCCCCGCCGGAGCAGAAGCCGGTGGTGGCGGGGCCGGCGTTCCCGGACCTGGCGGCGTACTACGACCGGCCGTCGGACTCGATGCCGCGGCTGCACGCCGACTCGAGCTGCTCGGAGCAGGTGCTGTCGCCGGAGTTCGCGTGCGAGGTGCAGAGCCAGCCCAAGATCAGCGAGTGGGAGCGCACCTTCGCCACCGTCGGGCCCATCAACCCCGCCGCCTCCATCCTCGACCCCGCCGGCTCCGGCGGCCTCGGCGGCCTCGGCGGCGGCGGCAGCGACCCCCTCCTCCAGGACATCCTCATGTACTGGGGCAAGCCATTCTAGACGACCAAAAAAAAAAAAAAACAACCGCATTGGCAGCAATGGTGTCACTGAACACCGTGCAGGCTAGCTAGCTTCATGGCCGGTGAACTTTGACTCAGGCGAGCCGCCGGAGTTGACTCAAAGATAATTAAAAGAAGTGTTTTAAGTGGATTGGATTGGATTAGACAGAGGAGATGAGGACTCGAGAAAGGCGGCGATGAGACCGTGGTTGGGGGGACCCTGGCCTGGACTGAACGACGACGAGGCAGCAGCAGAAAGATGGTGCAATTGCATCGGGTGGCATGTCAGTGTGTGTGTATAGTGGCATGTACATAGTACATGGTGATTGATTCGGTATACAGGGGGCTAGCTTTCCTGTTTCTGTTTCTTCATTGGTTAATTATTACTCCCATTATAAGGTCTTCTTCAGGGTTGCTAGCTTAATTAATTAATTAATTAGCCCAGTGGTTGAAGTGTAAGTCAAAATTCATCAAGTCAGAGACTGGAATAATACAATACAGTACTGTAGATATCTTTTTTTTTTTGGTAGATGCTGATGTCTGTTTGTTAGCAATGGAAAATAAAATGATGGAACCATGCGAGGAAAAAGCGTGGAATTAATACAACAGACGCATTTATTTTCCAACCGAGCTGCACATCACTACAGCTGGAGATGATCGATCAGATGAGCGACCAAGCAAAATAAGCTTGGGCGCGAAGGAAATCAAACGTGCCGCTCTGGGCGGAGTGAGGAAAAAACGTGAGGAATTGGAGTGCGCGCGTAGACGACATATGGGAGAGACGGGTGGATATGCACGCATTGAACGATGAGTGGTCGGTCGGTTCAATGCGCACGTGTGGGGGGCGGACGTAGTTTTGTCTTTGAGGGTTCATGGGGCGGTGCGGTTGACGACGTTCTACATGCGGCCTCGTGCTTATCGCCTCCGGTTAGCCGGCGTGTTCCCATTCCTGTTCTCCAGGATATGCTCCTGCTATACTCTCTCC
>LOC_Os01g66120.3
GTCGCTTGCCCCCGGCTCTATAAATGCCCGGCTCCTCACCCGGAACAAGTTTCAAGCCCTCCTCTCCTCTTCCCAACACTAGTAGGATAAAGCCACAGAGAGAGCAGTAGTAGTAGCGAGCTCGCCGGAGAACGGACGATCACCGGAGAAGGGGGAGAGAGATGAGCGGCGGTCAGGACCTGCAGCTGCCGCCGGGGTTCCGGTTCCACCCGACGGACGAGGAGCTGGTGATGCACTACCTCTGCCGCCGCTGCGCCGGCCTCCCCATCGCCGTCCCCATCATCGCCGAGATCGACCTCTACAAGTTCGATCCATGGCAGCTTCCCCGGATGGCGCTGTACGGAGAGAAGGAGTGGTACTTCTTCTCCCCGCGAGACCGCAAGTACCCGAACGGGTCGCGGCCGAACCGCGCCGCCGGGTCGGGGTACTGGAAGGCGACCGGCGCCGACAAGCCGGTGGGCTCGCCGAAGCCGGTGGCGATCAAGAAGGCCCTCGTCTTCTACGCCGGCAAGGCGCCCAAGGGCGAGAAGACCAACTGGATCATGCACGAGTACCGCCTCGCCGACGTCGACCGCTCCGCCCGCAAGAAGAACAGCCTCAGGTTGGATGATTGGGTGCTGTGCCGGATTTACAACAAGAAGGGCGGGCTGGAGAAGCCGCCGGCCGCGGCGGTGGCGGCGGCGGGGATGGTGAGCAGCGGCGGCGGCGTCCAGAGGAAGCCGATGGTGGGGGTGAACGCGGCGGTGAGCTCCCCGCCGGAGCAGAAGCCGGTGGTGGCGGGGCCGGCGTTCCCGGACCTGGCGGCGTACTACGACCGGCCGTCGGACTCGATGCCGCGGCTGCACGCCGACTCGAGCTGCTCGGAGCAGGTGCTGTCGCCGGAGTTCGCGTGCGAGGTGCAGAGCCAGCCCAAGATCAGCGAGTGGGAGCGCACCTTCGCCACCGTCGGGCCCATCAACCCCGCCGCCTCCATCCTCGACCCCGCCGGCTCCGGCGGCCTCGGCGGCCTCGGCGGCGGCGGCAGCGACCCCCTCCTCCAGGACATCCTCATGTACTGGGGCAAGCCATTCTAGACGACCAAAAAAAAAAAAAAACAACCGCATTGGCAGCAATGGTGTCACTGAACACCGTGCAGGCTAGCTAGCTTCATGGCCGGTGAACTTTGACTCAGGCGAGCCGCCGGAGTTGACTCAAAGATAATTAAAAGAAGTGTTTTAAGTGGATTGGATTGGATTAGACAGAGGAGATGAGGACTCGAGAAAGGCGGCGATGAGACCGTGGTTGGGGGGACCCTGGCCTGGACTGAACGACGACGAGGCAGCAGCAGAAAGATGGTGCAATTGCATCGGGTGGCATGTCAGTGTGTGTGTATAGTGGCATGTACATAGTACATGGTGATTGATTCGGTATACAGGGGGCTAGCTTTCCTGTTTCTGTTTCTTCATTGGTTAATTATTACTCCCATTATAAGGTCTTCTTCAGGGTTGCTAGCTTAATTAATTAATTAATTAGCCCAGTGGTTGAAGTGTAAGTCAAAATTCATCAAGTCAGAGACTGGAATAATACAATACAGTACTGTAGATATCTTTTTTTTTTTGGTAGATGCTGATGTCTGTTTGTTAGCAATGGAAAATAAAATGATGGAACCATGCGAGGAAAAAGCGTGGAATTAATACAACAGACGCATTTATTTTCCAACCGAGCTGCACATCACTACAGCTGGAGATGATCGATCAGATGAGCGACCAAGCAAAATAAGCTTGGGCGCGAAGGAAATCAAACGTGCCGCTCTGGGCGGAGTGAGGAAAAAACGTGAGGAATTGGAGTGCGCGCGTAGACGACATATGGGAGAGACGGGTGGATATGCACGCATTGAACGATGAGTGGTCGGTCGGTTCAATGCGCACGTGTGGGGGGCGGACGTAGTTTTGTCTTTGAGGGTTCATGGGGCGGTGCGGTTGACGACGTTCTACATGCGGCCTCGTGCTTATCGCCTCCGGTTAGCCGGCGTGTTCCCATTCCTGTTCTCCAGGATATGCTCCTGCTATACTCTCTCC

CDS Sequence

>LOC_Os01g66120.1
ATGAGCGGCGGTCAGGACCTGCAGCTGCCGCCGGGGTTCCGGTTCCACCCGACGGACGAGGAGCTGGTGATGCACTACCTCTGCCGCCGCTGCGCCGGCCTCCCCATCGCCGTCCCCATCATCGCCGAGATCGACCTCTACAAGTTCGATCCATGGCAGCTTCCCCGGATGGCGCTGTACGGAGAGAAGGAGTGGTACTTCTTCTCCCCGCGAGACCGCAAGTACCCGAACGGGTCGCGGCCGAACCGCGCCGCCGGGTCGGGGTACTGGAAGGCGACCGGCGCCGACAAGCCGGTGGGCTCGCCGAAGCCGGTGGCGATCAAGAAGGCCCTCGTCTTCTACGCCGGCAAGGCGCCCAAGGGCGAGAAGACCAACTGGATCATGCACGAGTACCGCCTCGCCGACGTCGACCGCTCCGCCCGCAAGAAGAACAGCCTCAGGTTGGATGATTGGGTGCTGTGCCGGATTTACAACAAGAAGGGCGGGCTGGAGAAGCCGCCGGCCGCGGCGGTGGCGGCGGCGGGGATGGTGAGCAGCGGCGGCGGCGTCCAGAGGAAGCCGATGGTGGGGGTGAACGCGGCGGTGAGCTCCCCGCCGGAGCAGAAGCCGGTGGTGGCGGGGCCGGCGTTCCCGGACCTGGCGGCGTACTACGACCGGCCGTCGGACTCGATGCCGCGGCTGCACGCCGACTCGAGCTGCTCGGAGCAGGTGCTGTCGCCGGAGTTCGCGTGCGAGGTGCAGAGCCAGCCCAAGATCAGCGAGTGGGAGCGCACCTTCGCCACCGTCGGGCCCATCAACCCCGCCGCCTCCATCCTCGACCCCGCCGGCTCCGGCGGCCTCGGCGGCCTCGGCGGCGGCGGCAGCGACCCCCTCCTCCAGGACATCCTCATGTACTGGGGCAAGCCATTCTAG
>LOC_Os01g66120.3
ATGAGCGGCGGTCAGGACCTGCAGCTGCCGCCGGGGTTCCGGTTCCACCCGACGGACGAGGAGCTGGTGATGCACTACCTCTGCCGCCGCTGCGCCGGCCTCCCCATCGCCGTCCCCATCATCGCCGAGATCGACCTCTACAAGTTCGATCCATGGCAGCTTCCCCGGATGGCGCTGTACGGAGAGAAGGAGTGGTACTTCTTCTCCCCGCGAGACCGCAAGTACCCGAACGGGTCGCGGCCGAACCGCGCCGCCGGGTCGGGGTACTGGAAGGCGACCGGCGCCGACAAGCCGGTGGGCTCGCCGAAGCCGGTGGCGATCAAGAAGGCCCTCGTCTTCTACGCCGGCAAGGCGCCCAAGGGCGAGAAGACCAACTGGATCATGCACGAGTACCGCCTCGCCGACGTCGACCGCTCCGCCCGCAAGAAGAACAGCCTCAGGTTGGATGATTGGGTGCTGTGCCGGATTTACAACAAGAAGGGCGGGCTGGAGAAGCCGCCGGCCGCGGCGGTGGCGGCGGCGGGGATGGTGAGCAGCGGCGGCGGCGTCCAGAGGAAGCCGATGGTGGGGGTGAACGCGGCGGTGAGCTCCCCGCCGGAGCAGAAGCCGGTGGTGGCGGGGCCGGCGTTCCCGGACCTGGCGGCGTACTACGACCGGCCGTCGGACTCGATGCCGCGGCTGCACGCCGACTCGAGCTGCTCGGAGCAGGTGCTGTCGCCGGAGTTCGCGTGCGAGGTGCAGAGCCAGCCCAAGATCAGCGAGTGGGAGCGCACCTTCGCCACCGTCGGGCCCATCAACCCCGCCGCCTCCATCCTCGACCCCGCCGGCTCCGGCGGCCTCGGCGGCCTCGGCGGCGGCGGCAGCGACCCCCTCCTCCAGGACATCCTCATGTACTGGGGCAAGCCATTCTAG

Protein Sequence

>LOC_Os01g66120.1
MSGGQDLQLPPGFRFHPTDEELVMHYLCRRCAGLPIAVPIIAEIDLYKFDPWQLPRMALYGEKEWYFFSPRDRKYPNGSRPNRAAGSGYWKATGADKPVGSPKPVAIKKALVFYAGKAPKGEKTNWIMHEYRLADVDRSARKKNSLRLDDWVLCRIYNKKGGLEKPPAAAVAAAGMVSSGGGVQRKPMVGVNAAVSSPPEQKPVVAGPAFPDLAAYYDRPSDSMPRLHADSSCSEQVLSPEFACEVQSQPKISEWERTFATVGPINPAASILDPAGSGGLGGLGGGGSDPLLQDILMYWGKPF*
>LOC_Os01g66120.3
MSGGQDLQLPPGFRFHPTDEELVMHYLCRRCAGLPIAVPIIAEIDLYKFDPWQLPRMALYGEKEWYFFSPRDRKYPNGSRPNRAAGSGYWKATGADKPVGSPKPVAIKKALVFYAGKAPKGEKTNWIMHEYRLADVDRSARKKNSLRLDDWVLCRIYNKKGGLEKPPAAAVAAAGMVSSGGGVQRKPMVGVNAAVSSPPEQKPVVAGPAFPDLAAYYDRPSDSMPRLHADSSCSEQVLSPEFACEVQSQPKISEWERTFATVGPINPAASILDPAGSGGLGGLGGGGSDPLLQDILMYWGKPF*