Gene Details:

• MSU gene ID: LOC_Os01g66120
• RAPdb gene ID: Os01g0884300
• Gene Symbol: OsNAC6 SNAC2
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Our results imply that OsNAC6, besides having a role in plant adaptation to abiotic stresses, also integrates signals derived from both abiotic and biotic stresses.
• Transgenic rice plants over-expressing OsNAC6 constitutively exhibited growth retardation and low reproductive yields.
• The root phenotype of OsNAC6 knock-out seedlings was observed to be similar to that of the OsHDAC1(OE) seedlings.
• Conversely, the root phenotype of the OsNAC6 overexpressors was similar to that of the OsHDAC1 knock-out seedlings.
• These observations indicate that OsHDAC1 negatively regulates the OsNAC6 gene that primarily mediates the alteration in the root growth of the OsHDAC1(OE) seedlings.
• The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice.
• OsNAC6 was also induced by jasmonic acid (JA), a plant hormone that activates defense responses against herbivores and pathogens.
• Expression of OsNAC6 is induced by abiotic stresses, including cold, drought and high salinity.
• The growth of these plants was similar to that of control plants, whereas the growth of OsNAC6-overexpressing transgenic plants was retarded.
• Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different.
• These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice.
• Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice.
• Northern blot and SNAC2 promoter activity analyses suggest that SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment.
• Here we show that OsNAC6 is induced by cold, salt, drought and abscisic acid (ABA).
• By utilizing stress-inducible promoters, such as the OsNAC6 promoter, it is hoped that stress-inducible over-expression of OsNAC6 in rice can improve stress tolerance by suppressing the negative effects of OsNAC6 on growth under normal growth conditions.
• OsNAC6 gene expression is also induced by wounding and blast disease.
• The OsNAC6 gene is a member of the NAC transcription factor gene family in rice.
• Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice.
• The results of microarray analysis revealed that many genes that are inducible by abiotic and biotic stresses were upregulated in rice plants over-expressing OsNAC6.
• Collectively, these results indicate that OsNAC6 functions as a transcriptional activator in response to abiotic and biotic stresses in plants.
• The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance.
• Multiyear drought field tests demonstrated that the grain yield of OsNAC6 root-specific overexpressing transgenic rice lines was less affected by drought stress than were non-transgenic controls.
• Collectively, OsNAC6 orchestrates novel molecular drought tolerance mechanisms and has potential for the biotechnological development of high-yielding crops under water limiting conditions.
• Genome-wide analyses of loss- and gain-of-function mutants revealed that OsNAC6 up-regulates the expression of direct target genes involved in membrane modification, nicotianamine (NA) biosynthesis, glutathione relocation, 3’-phophoadenosine 5’-phosphosulfate accumulation, and glycosylation, which represent multiple drought tolerance pathways.

Function-related keywords:

• biotic-stress ,  yield ,  seedling ,  jasmonic-acid ,  salinity ,  growth ,  transcription-factor ,  reproductive ,  salt ,  defense-response ,  drought ,  disease ,  blast-disease ,  salt-tolerance ,  defense ,  abiotic-stress ,  jasmonic ,  root ,  blast ,  grain ,  development ,  tolerance ,  grain-yield ,  drought-tolerance ,  stress ,  drought-stress

Literature:

• The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice .  DOI:  10.1007/s00438-010-0557-0 ;  PMID:  20632034
• OsNAC6, a member of the NAC gene family, is induced by various stresses in rice .  DOI:  10.1266/ggs.80.135 ;  PMID:  16172526
• Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice .  DOI:  10.1007/s11103-008-9309-5 ;  PMID:  18273684
• Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice .  DOI:  10.1111/j.1365-313X.2007.03168.x ;  PMID:  17587305
• The histone deacetylase OsHDAC1 epigenetically regulates the OsNAC6 gene that controls seedling root growth in rice .  DOI:  10.1111/j.1365-313X.2009.03908.x ;  PMID:  19453457
• The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance .  DOI:  10.1111/pbi.12673 ;  PMID:  27892643
• Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome .  DOI:  10.1590/1678-4685-GMB-2022-0190 ;  PMID:  37144919

Related News:

Gene Resources:

• NCBI ID: AK068392
• UniProt accessions:

Sequences: