Information report for OsABF1;OsABI5
Gene Details
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Functional Descriptions
- Interestingly, D-allose also up-regulated expression of OsABF1, encoding a conserved bZIP transcription factor in ABA signaling, in rice.
- The homozygous T-DNA insertional mutants OsABF1-1 and OsABF1-2 were more sensitive in response to drought and salinity treatments than wild type plants.
- Expression of the OsABI5 gene was induced by abscisic acid (ABA) and high salinity, and down-regulated by drought and cold (4 degrees C) in seedlings.
- These findings indicated that the phosphorylation of D-allose at C6 by hexokinase is essential and OsABF1 is involved in the signal transduction for D-allose-induced growth inhibition.
- Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L.
- Complementation analysis revealed that the expression of OsABI5 driven by the 35S promoter could rescue ABA-insensitivity of abi5-1 during seed germination and result in hypersensitivity to ABA.
- Complementation tests and ABA (abscisic acid) hypersensitivity of Arabidopsis transgenic lines revealed the redundant function of OsABI5 splicing variants in ABA signaling.
- These results suggest that OsABI5 variants may have overlapping and distinct functions to fine tune gene expression in ABA signaling as transcription factors together with OsVP1.
- A bZIP-type transcription factor, OsABI5, undergoes alternative splicing.
- Characterization of alternative splicing products of bZIP transcription factors OsABI5.
- A bZIP transcription factor, OsABI5, was isolated from the panicle of Oryza sativa L.
- Over-expression of OsABI5 in rice conferred high sensitivity to salt stress.
- In our current study in rice, we have newly isolated and characterized the OsABF1 (Oryza sativa ABA responsive element binding factor 1) gene that encodes a bZIP transcription factor.
- The bZIP transcription factor OsABF1 is an ABA responsive element binding factor that enhances abiotic stress signaling in rice.
- In a yeast experiment, OsABF1 was shown to bind to ABA responsive elements (ABREs) and its N-terminal region was necessary to transactivate the downstream reporter gene.
- Furthermore, the upregulated expression of some ABA/stress-regulated genes in response to ABA treatment was suppressed in these OsABF1 mutants.
- Our current results thus suggest that OsABF1 is involved in abiotic stress responses and ABA signaling in rice.
- Repression of OsABI5 promoted stress tolerance and resulted in low fertility of rice.
- These results suggested that OsABI5 could regulate the adaptive stress response and plant fertility of rice as a transcription factor.
- A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance.
- Molecular and genetic analyses demonstrate that a drought regime enhances expression of the OsABF1 gene, which indirectly suppresses expression of the Early heading date 1 (Ehd1) gene that encodes a key activator of rice flowering.
- Furthermore, we identified a drought inducible gene named OsWRKY104 that is under the direct regulation of OsABF1.
- A Drought-inducible bZIP Transcription Factor OsABF1 Delays Reproductive Timing in Rice.
- The OsABF1 transcription factor improves drought tolerance by activating the transcription of COR413-TM1 in rice.
- In this study, we show that when overexpressed in transgenic rice (Oryza sativa), the bZIP transcription factor OsABF1 confers distinctly different drought-tolerance phenotypes when tethered to the transcriptional activator VP16 versus the transcriptional repressor EAR.
- Analysis of the overlap between the RNA-sequencing and chromatin immunoprecipitation-sequencing data identified 242 OsABF1 target genes involved in multiple aspects of the drought response.
- In addition, OsABF1 directly regulates the expression of the protein phosphatase 2C (OsPP48 and OsPP108) and bZIP (OsbZIP23, OsbZIP46, and OsbZIP72) genes, thus forming a complex feedback circuit in the drought/abscisic acid signaling pathway.
- It is suggested that OsABF1 recruits RRC2-mediated H3K27me3 deposition on the SD1 promoter, thus epigenetically silencing SD1 to maintain the GA homeostasis for growth and seed germination.
- OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (Oryza sativa L.).
- Here, we report the functional characterization of a GA-inducible OsABF1 in GA biosynthesis underpinning plant height and seed germination.
- Overexpression of OsABF1 produced a typical GA-deficient phenotype with semi-dwarf and retarded seed germination.
- Meanwhile, the phenotypes could be rescued by exogenous GA(3), suggesting that OsABF1 is a key regulator of GA homeostasis.
- These findings shed new insight into the functions of OsABF1 and regulatory mechanism underlying GA homeostasis in rice.
- OsABF1 could directly suppress the transcription of green revolution gene SD1, thus reducing the endogenous GA level in rice.
- Furthermore, the upregulated expression of OsABI5 was detected in wild type (WT) under drought stress, irrespective of MT treatment, whereas OsABI5 was significantly downregulated in sgt1 and sgt1abi5 mutants.
- Further analysis indicated that OsABI5 directly regulated the transcriptional expression of OsAPX1, whose encoding products promoted H(2) O(2) scavenging to maintain redox homeostasis, which was essential for germination.
- In addition, the induction of OsPRR95 by ABA partly required a functional OsRCAR10, and the ABA responsive element (ABRE)-binding factor ABSCISIC ACID INSENSITIVE5 (OsABI5) bound directly to the promoter of OsPRR95 and activated its expression, thus establishing a regulatory feedback loop between OsPRR95, OsRCAR10 and OsABI5.
Functional Keywords
- transcription-factor , salinity , drought , growth , seed-germination , ABA , panicle , salt , seed , salt-stress , fertility , abiotic-stress , seedling , reproductive , heading-date , tolerance , drought-tolerance , transcriptional-activator , abscisic-acid , protein-phosphatase , gibberellin , homeostasis , ga , height , plant-height , Gibberellin , GA , GA-biosynthesis , gibberellin-biosynthesis , drought-stress , redox-homeostasis
Literature and News
- Characterization of alternative splicing products of bZIP transcription factors OsABI5 . DOI: 10.1016/j.bbrc.2007.05.226 ; PMID: 17604002
- Phosphorylation-mediated regulation of a rice ABA responsive element binding factor . DOI: 10.1016/j.phytochem.2010.10.005 ; PMID: 21055780
- Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L . DOI: 10.1007/s00425-013-1853-9 ; PMID: 23397192
- A rice dehydration-inducible SNF1-related protein kinase 2 phosphorylates an abscisic acid responsive element-binding factor and associates with ABA signaling . DOI: 10.1007/s11103-006-9079-x ; PMID: 16977424
- The bZIP transcription factor OsABF1 is an ABA responsive element binding factor that enhances abiotic stress signaling in rice . DOI: 10.1007/s11103-009-9592-9 ; PMID: 20039193
- Genome-wide analysis of basic leucine zipper transcription factor families in Arabidopsis thaliana, Oryza sativa and Populus trichocarpa . DOI: 10.1007/s11741-009-0216-3
- Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice . DOI: 10.1104/pp.111.190389 ; PMID: 22301130
- Rice ABI5-Like1 regulates abscisic acid and auxin responses by affecting the expression of ABRE-containing genes . DOI: 10.1104/pp.111.173427 ; PMID: 21546455
- A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance . DOI: 10.1007/s11103-008-9298-4 ; PMID: 18236009
- A Drought-Inducible Transcription Factor Delays Reproductive Timing in Rice . DOI: 10.1104/pp.16.01691 ; PMID: 26945049
- The OsABF1 transcription factor improves drought tolerance by activating the transcription of COR413-TM1 in rice . DOI: 10.1093/jxb/erx260 ; PMID: 28981779
- Histone deacetylase HDA710 controls salt tolerance by regulating ABA signaling in rice . DOI: 10.1111/jipb.13042 ; PMID: 33289304
- Transcriptional and post-transcriptional regulation of heading date in rice . DOI: 10.1111/nph.17158 ; PMID: 33341945
- Dynamic genome-wide association analysis and identification of candidate genes involved in anaerobic germination tolerance in rice . DOI: 10.1186/s12284-020-00444-x ; PMID: 33409869
- OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (Oryza sativa L.) . DOI: 10.3390/ijms222212220 ; PMID: 34830102
- Melatonin Promotes SGT1-Involved Signals to Ameliorate Drought Stress Adaption in Rice . DOI: 10.3390/ijms23020599 ; PMID: 35054782
- OsSGT1 promotes melatonin-ameliorated seed tolerance to chromium stress by affecting the OsABI5-OsAPX1 transcriptional module in rice . DOI: 10.1111/tpj.15937 ; PMID: 35942609
- A regulatory loop establishes the link between the circadian clock and abscisic acid signaling in rice . DOI: 10.1093/plphys/kiac548 ; PMID: 36493391
Gene Resources
- UniProt: Q0JHF1
- EMBL: AK067919, AP003286, AP008207
- AlphaFoldDB: Q0JHF1
- EnsemblPlants: Os01t0867300-01
- Gramene: Os01t0867300-01
- KEGG: dosa:Os01g0867300, osa:4325078
- Orthologous matrix: IRRAHSM
- InterPro: IPR004827, IPR043452, IPR046347
- PANTHER: PTHR22952, PTHR22952:SF392
- SUPFAM: SSF57959
- PROSITE: PS00036, PS50217
- Gene3D: 1.20.5.170
- OrthoDB: Q0JHF1
- SWISS-MODEL: Q0JHF1
- Conserved Domain Database: cd14707
- eggNOG: ENOG502RXGY
Sequences
cDNA Sequence
- >LOC_Os01g64730.1
ATTAACCTCTAAACCATAAAGTCCCCCCGTCTCATGTCGTCTCATCACTCACTTATAATCACCCCCATCCACTTAATCACGCTCTCAACGCTGCTGTGTCACGTCACGTCACGCGCGGCTCCGTAATAAGACCACCAACCTCCTCCTCCTCCCTCCCACGTTTGGAGCTCTCGTCTCGTCTCGCGGCCTCGAGCCCTCGACCAACCAACCACCCGCTTCTTCTCTCCCGTCTCCCGAAACCTCCGAGAGCTCAGAAGAAAAAAGAAGAAAAAAAAGAGAAAAAAAAACTGGTGGAAACTGGAAGGTTTGCGGCGGAAACGAGGGGGAGGGATGGTGGCGAGGAGGGCGTAGGCGGGGGGTGGGGGTGAGGCGGATGATGGCGTCGAGGGTGATGGCGTCGTCGTCGCCGTCGCACACGGCATCGGATCTCGCGCGGTTCGCGGCGGGGAGGGGTGGGGGCGGGAGCGCGGGGCTGGGGTCGATGAACGTGGAGGAGATCCTCCGCGGCATCTACGCCGACATGCCGACGCCGGCGCTGCCCCTCGTCGGCGGTGACAGGCCGATGTCGCCGCTCCCGGCGCCGGATGTCGCCGCCGCGCCGCGGACGGCGGAGGAGGTCTGGAAGGAGATAACTGGTGCGGGCGTCGCCGCCGCCGCCGGCGGCGTGGTGCCCCCTGCTGCTGCTGCTGCTGCTGCCCCGGCCGTCGTCGCTGGCGCTGGCGCTGGCACCGGCGCGGAGATGACGCTGGAGGACTTTCTGGCGAGGGAGGGCGCGGTGAAGGAAGACGAGGCTGTGGTCACCGATCCCTCGGCGGCCAAGGGGCAGGTGGTGATGGGGTTCCTGAACGGGGCAGAGGTCACCGGAGGCGTCACCGGCGGCAGGAGCAGGAAGAGGCACCTGATGGATCCGATGGACCGCGCCGCGATGCAGCGGCAGAAGCGAATGATCAAAAATCGCGAGTCGGCGGCGAGGTCACGGGAGAGGAAGCAGGCCTATATTGCTGAGCTCGAGTCGCTGGTCACGCAACTCGAGGAGGAGAACGCCAAGATGTTCAAAGAACAGGAGGAGCAACATCAGAAACGACTTAAAGAGCTCAAGGAAATGGTTGTTCCAGTCATCATTAGGAAAACGTCAGCACGAGATCTTAGAAGAACAAACTCGATGGAGTGGTAGACGTGAGCCAAGCTCAGTTGCCATCCCCATACAGTTTATGTAGCATTTGCTCCTGTTTTGTCAATTCTTCTGTTTTATCGGCAGTAGCCTAGTTGCGGTGAATACATGAGTATTTAGGTGACACGAAGCTTAAATGTCTGGAGTCATATGCTACGGAGGGGAGAGTGCGTACGAGCTACAAAAGCAATCATATGTGTAGGGAGGGGAGAGTGCATATGAGCTATAAAAGCCATGTTCCAATTTGTCGCGGGAGTGTATTCTGTCCCATTTGTTGTCTGTTTCATTTTCAGTTTAACAATTAGGTGTAGTGAGGAAGAATTACGAGTACTGAGTAGTACTAAACAGGGTGACTCTAGGTATGCTGATATGATATCTAGTTAGGCATTACTTCTGATTCTCTGTTTGGTGCCACGACTCAAATTGTCTGTACATACACGTTGGATGTAAAGTCACATTATCAAGTGAAATCATGATGTATTTCCTCCAGAATTTTTTTT - >LOC_Os01g64730.2
ATTAACCTCTAAACCATAAAGTCCCCCCGTCTCATGTCGTCTCATCACTCACTTATAATCACCCCCATCCACTTAATCACGCTCTCAACGCTGCTGTGTCACGTCACGTCACGCGCGGCTCCGTAATAAGACCACCAACCTCCTCCTCCTCCCTCCCACGTTTGGAGCTCTCGTCTCGTCTCGCGGCCTCGAGCCCTCGACCAACCAACCACCCGCTTCTTCTCTCCCGTCTCCCGAAACCTCCGAGAGCTCAGAAGAAAAAAGAAGAAAAAAAAGAGAAAAAAAAACTGGTGGAAACTGGAAGGTTTGCGGCGGAAACGAGGGGGAGGGATGGTGGCGAGGAGGGCGTAGGCGGGGGGTGGGGGTGAGGCGGATGATGGCGTCGAGGGTGATGGCGTCGTCGTCGCCGTCGCACACGGCATCGGATCTCGCGCGGTTCGCGGCGGGGAGGGGTGGGGGCGGGAGCGCGGGGCTGGGGTCGATGAACGTGGAGGAGATCCTCCGCGGCATCTACGCCGACATGCCGACGCCGGCGCTGCCCCTCGTCGGCGGTGACAGGCCGATGTCGCCGCTCCCGGCGCCGGATGTCGCCGCCGCGCCGCGGACGGCGGAGGAGGTCTGGAAGGAGATAACTGGTGCGGGCGTCGCCGCCGCCGCCGGCGGCGTGGTGCCCCCTGCTGCTGCTGCTGCTGCTGCCCCGGCCGTCGTCGCTGGCGCTGGCGCTGGCACCGGCGCGGAGATGACGCTGGAGGACTTTCTGGCGAGGGAGGGCGCGGTGAAGGAAGACGAGGCTGTGGTCACCGATCCCTCGGCGGCCAAGGGGCAGGTGGTGATGGGGTTCCTGAACGGGGCAGAGGTCACCGGAGGCGTCACCGGCGGCAGGAGCAGGAAGAGGCACCTGATGGATCCGATGGACCGCGCCGCGATGCAGCGGCAGAAGCGAATGATCAAAAATCGCGAGTCGGCGGCGAGGTCACGGGAGAGGAAGCAGGCCTATATTGCTGAGCTCGAGTCGCTGGTCACGCAACTCGAGGAGGAGAACGCCAAGATGTTCAAAGAACAGGAGGAGCAACATCAGAAACGACTTAAAGAGCTCAAGGAAATGGTTGTTCCAGTCATCATTAGGAAAACGTCAGCACGAGATCTTAGAAGAACAAACTCGATGGAGTGGTAGACGTGAGCCAAGCTCAGTTGCCATCCCCATACAGTTTATGTAGCATTTGCTCCTGTTTTGTCAATTCTTCTGTTTTATCGGCAGTAGCCTAGTTGCGGTGAATACATGAGTATTTAGGTGACACGAAGCTTAAATGTCTGGAGTCATATGCTACGGAGGGGAGAGTGCGTACGAGCTACAAAAGCAATCATATGTGTAGGGAGGGGAGAGTGCATATGAGCTATAAAAGCCATGTTCCAATTTGTCGCGGGAGTGTATTCTGTCCCATTTGTTGTCTGTTTCATTTTCAGTTTAACAATTAGGTGTAGTGAGGAAGAATTACGAGTACTGAGTAGTACTAAACAGGGTGACTCTAGGTATGCTGATATGATATCTAGTTAGGCATTACTTCTGATTCTCTGTTTGGTGCCACGACTCAAATTGTCTGTACATACACGTTGGATGTAAAGTCACATTATCAAGTGAAATCATGATGTATTTCCTCCAGAATTTTTTTT
CDS Sequence
- >LOC_Os01g64730.1
ATGATGGCGTCGAGGGTGATGGCGTCGTCGTCGCCGTCGCACACGGCATCGGATCTCGCGCGGTTCGCGGCGGGGAGGGGTGGGGGCGGGAGCGCGGGGCTGGGGTCGATGAACGTGGAGGAGATCCTCCGCGGCATCTACGCCGACATGCCGACGCCGGCGCTGCCCCTCGTCGGCGGTGACAGGCCGATGTCGCCGCTCCCGGCGCCGGATGTCGCCGCCGCGCCGCGGACGGCGGAGGAGGTCTGGAAGGAGATAACTGGTGCGGGCGTCGCCGCCGCCGCCGGCGGCGTGGTGCCCCCTGCTGCTGCTGCTGCTGCTGCCCCGGCCGTCGTCGCTGGCGCTGGCGCTGGCACCGGCGCGGAGATGACGCTGGAGGACTTTCTGGCGAGGGAGGGCGCGGTGAAGGAAGACGAGGCTGTGGTCACCGATCCCTCGGCGGCCAAGGGGCAGGTGGTGATGGGGTTCCTGAACGGGGCAGAGGTCACCGGAGGCGTCACCGGCGGCAGGAGCAGGAAGAGGCACCTGATGGATCCGATGGACCGCGCCGCGATGCAGCGGCAGAAGCGAATGATCAAAAATCGCGAGTCGGCGGCGAGGTCACGGGAGAGGAAGCAGGCCTATATTGCTGAGCTCGAGTCGCTGGTCACGCAACTCGAGGAGGAGAACGCCAAGATGTTCAAAGAACAGGAGGAGCAACATCAGAAACGACTTAAAGAGCTCAAGGAAATGGTTGTTCCAGTCATCATTAGGAAAACGTCAGCACGAGATCTTAGAAGAACAAACTCGATGGAGTGGTAG - >LOC_Os01g64730.2
ATGATGGCGTCGAGGGTGATGGCGTCGTCGTCGCCGTCGCACACGGCATCGGATCTCGCGCGGTTCGCGGCGGGGAGGGGTGGGGGCGGGAGCGCGGGGCTGGGGTCGATGAACGTGGAGGAGATCCTCCGCGGCATCTACGCCGACATGCCGACGCCGGCGCTGCCCCTCGTCGGCGGTGACAGGCCGATGTCGCCGCTCCCGGCGCCGGATGTCGCCGCCGCGCCGCGGACGGCGGAGGAGGTCTGGAAGGAGATAACTGGTGCGGGCGTCGCCGCCGCCGCCGGCGGCGTGGTGCCCCCTGCTGCTGCTGCTGCTGCTGCCCCGGCCGTCGTCGCTGGCGCTGGCGCTGGCACCGGCGCGGAGATGACGCTGGAGGACTTTCTGGCGAGGGAGGGCGCGGTGAAGGAAGACGAGGCTGTGGTCACCGATCCCTCGGCGGCCAAGGGGCAGGTGGTGATGGGGTTCCTGAACGGGGCAGAGGTCACCGGAGGCGTCACCGGCGGCAGGAGCAGGAAGAGGCACCTGATGGATCCGATGGACCGCGCCGCGATGCAGCGGCAGAAGCGAATGATCAAAAATCGCGAGTCGGCGGCGAGGTCACGGGAGAGGAAGCAGGCCTATATTGCTGAGCTCGAGTCGCTGGTCACGCAACTCGAGGAGGAGAACGCCAAGATGTTCAAAGAACAGGAGGAGCAACATCAGAAACGACTTAAAGAGCTCAAGGAAATGGTTGTTCCAGTCATCATTAGGAAAACGTCAGCACGAGATCTTAGAAGAACAAACTCGATGGAGTGGTAG
Protein Sequence
- >LOC_Os01g64730.1
MMASRVMASSSPSHTASDLARFAAGRGGGGSAGLGSMNVEEILRGIYADMPTPALPLVGGDRPMSPLPAPDVAAAPRTAEEVWKEITGAGVAAAAGGVVPPAAAAAAAPAVVAGAGAGTGAEMTLEDFLAREGAVKEDEAVVTDPSAAKGQVVMGFLNGAEVTGGVTGGRSRKRHLMDPMDRAAMQRQKRMIKNRESAARSRERKQAYIAELESLVTQLEEENAKMFKEQEEQHQKRLKELKEMVVPVIIRKTSARDLRRTNSMEW* - >LOC_Os01g64730.2
MMASRVMASSSPSHTASDLARFAAGRGGGGSAGLGSMNVEEILRGIYADMPTPALPLVGGDRPMSPLPAPDVAAAPRTAEEVWKEITGAGVAAAAGGVVPPAAAAAAAPAVVAGAGAGTGAEMTLEDFLAREGAVKEDEAVVTDPSAAKGQVVMGFLNGAEVTGGVTGGRSRKRHLMDPMDRAAMQRQKRMIKNRESAARSRERKQAYIAELESLVTQLEEENAKMFKEQEEQHQKRLKELKEMVVPVIIRKTSARDLRRTNSMEW*