Gene Details:

Functional Descriptions:

  • Reciprocal immunoprecipitation and pull-down assays indicated an interaction between SBDCP1 and starch synthase IIIa (SSIIIa), which was down-regulated at the protein level in the CS8 line.
  • Furthermore, binding by SBDCP1 inhibited SSIIIa starch polymerization activity in a non-competitive manner.
  • Surprisingly, artificial microRNA gene suppression of SBDCP1 restored protein expression levels of SSIIIa in the CS8 line resulting in starch with lower amylose content and increased amylopectin chains with a higher degree of polymerization.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g63810.1
    GCGATTCCCTCGTCCCACCCTGCTGAAAAAGGCCCGCCCGGAGCGCGAATGCATCCGTGCCGCGTCAAGCAACTCTCGGTGGTGTGGTGTCCACTGTGCAGCTCCCGTGCAGGCGTTCCGCTTTGATGGAAACCGCCGCGGCCGTGGTGTGCCGAGGCGGCGGGCTGCGCGCGCCGGCGCGTCGCGGTGGCAGTGACAGCAGCACGACGCGCGCGGGTGGCGTTGCTGCCTCCCCGGCGCCCGCCACCACCGCGCGACGGAGGCCGCTGCTCGTCGCGTCGCTCGGGGAGCCCCTCATCACGGCGCAACCCTTGTCGTCGTCCCTGGGAGATGGCGCCGCGGTACACGAGACGCTGGCTCGCTCCGATTCGGTGATCCCGTCGCTGAAGCCGTCTCACTGCGTTGATCACTCGGTCCAGGTTGACGCCGACGAGGAGACCGGCTCCACCAAGACACTCCCACCTCCTGATGACGTTCCTACGAAGACAGTGCACGTCAAATTCGTGCTGCAAAAGCGGTGCGCGTTCGGGCAGCGCTTCCTCGTCGTCGGCGACGTCGCGGCGCTCGGTCTCTGGAATCCGGCGAAGGCGGCTGCTCTGGATTGGTCCGAAGACCACGTGTGGACAGTGAAGAAGGAATTGCCAGCAGAAAGATCGATCGAGTTCAAGTTCTTGCTGCAAGATCGATCGGGACATGTCGAATGGCAGCACGGTCGTAACAGAATACTGCATGTAGCTGACACCTCGAACACCTTGATTGTCTGCGAGGACTGGGACGAGGCGAAGAATCAGCAAGTATCAGAAGAGATTGGTGACGCAGATGGGATATTTTCAGGAAGCGATGGCGTCTTCCAGGAAGATGAGCTTCAACTGGGTGAAGAACAAGAGACCAACAAGGGTGTGACTGTAGGTGTGGATGATGCAAAATCGGCTTTAGTCACTTATATTTACAGGGAAATGATGGGAGCAAACGACGCGATCCAGCCACAGTTAGCATTGGACAAACACCACAAGATTCCAGATGAGCTTAGTGGGGAGGCAAACATGGCAGCACAAGATGGCAATCACACTGCTACTGCAGCCGCAGCTTCTGGTTTTGCTGGAAGCAACGGTGAGGATGCCATCTTGCACAAGGAGGGCGATCCAGTAGAGAATAATAGGCTGGGGCTGGCCAGCATTTTCTTCAATGACATGGCCTGGACCAGGAAAGCCCTGCAGCAGTTGCTCCGAAGCCTTGGTTTCCAAATCGGCACGAGAAAAACATGAAGTAAGCATCAGAGACGCTGACCTGCAGTTTTGGCTGATCACACGGTCTCGCGGTGCCTTCATTCAAAACTCGAAAGCTTCTACATGCTGCAAACCTACAAGGAACAAGTGTATTGAGAACTGCAACAAAAAACCCTTTACGTTGCTAAGGTCTGCAACACCAGCAGTACTTGTTAGCAGTGCAAAAAGAGCAGGAAAGCTGTTAGCAGTGCAAGTATGTTTAGGACCAGTAATGACTAAGCTACCAAAAAACTCAATTAGTGAAATGCATATTGTGGTTAAATTTATCTGTTCGATAAATTCTGTAGAATACATCTAGAATAAGAGCCAGGAAAAGCTGAAAGTCTGGAGATTTGGCATAACATGTGCTCCATAACCACAACGTATAAGCTCAACCACATGTACTTTAATCCACCAGATTTACGTTCATTTCTCAATCATTTCAGTTGTAATGCTAATATGCTCTTGTTCTGGTGATCTAGTAAAAATGATGTCCATGATTTGCAATGCTTTCTTTAATTTACATAAGAAAGCTGGCAGCATTTAAAACATGTAAACGTGAAATATCAGCGTGGACATTCCACTTTAATTAAACTAACTTGTTTACAAATAACTGTATCCGTCAAGGAAACAGTCAATATTCATGACATACCCCTACCCCTACCTGTTTACAGCTATCCAACTACGCCAGTATCCTGTGAAGATTACAGCTTTGGTTGTATGGAGCAAACGGTCCAAGGTCAACAGAATCCACGATGCACCATGCCCAATTCTCTATAATTTATCACCTTCTAAACTCGGAGAGGCAAAAAACACTTTGCATGAAAATCATGGTTCAAGAGGCAGCCAGCCAACCATAATTGGA
  • >LOC_Os01g63810.2
    GCGATTCCCTCGTCCCACCCTGCTGAAAAAGGCCCGCCCGGAGCGCGAATGCATCCGTGCCGCGTCAAGCAACTCTCGGTGGTGTGGTGTCCACTGTGCAGCTCCCGTGCAGGCGTTCCGCTTTGATGGAAACCGCCGCGGCCGTGGTGTGCCGAGGCGGCGGGCTGCGCGCGCCGGCGCGTCGCGGTGGCAGTGACAGCAGCACGACGCGCGCGGGTGGCGTTGCTGCCTCCCCGGCGCCCGCCACCACCGCGCGACGGAGGCCGCTGCTCGTCGCGTCGCTCGGGGAGCCCCTCATCACGGCGCAACCCTTGTCGTCGTCCCTGGGAGATGGCGCCGCGGTACACGAGACGCTGGCTCGCTCCGATTCGGTGATCCCGTCGCTGAAGCCGTCTCACTGCGTTGATCACTCGGTCCAGGTTGACGCCGACGAGGAGACCGGCTCCACCAAGACACTCCCACCTCCTGATGACGTTCCTACGAAGACAGTGCACGTCAAATTCGTGCTGCAAAAGCGGTGCGCGTTCGGGCAGCGCTTCCTCGTCGTCGGCGACGTCGCGGCGCTCGGTCTCTGGAATCCGGCGAAGGCGGCTGCTCTGGATTGGTCCGAAGACCACGTGTGGACAGTGAAGAAGGAATTGCCAGCAGAAAGATCGATCGAGTTCAAGTTCTTGCTGCAAGATCGATCGGGACATGTCGAATGGCAGCACGGTCGTAACAGAATACTGCATGTAGCTGACACCTCGAACACCTTGATTGTCTGCGAGGACTGGGACGAGGCGAAGAATCAGCAAGTATCAGAAGAGATTGGTGACGCAGATGGGATATTTTCAGGAAGCGATGGCGTCTTCCAGGAAGATGAGCTTCAACTGGGTGAAGAACAAGAGACCAACAAGGGTGTGACTGTAGGTGTGGATGATGCAAAATCGGCTTTAGTCACTTATATTTACAGGGAAATGATGGGAGCAAACGACGCGATCCAGCCACAGTTAGCATTGGACAAACACCACAAGATTCCAGATGAGCTTAGTGGGGAGGCAAACATGGCAGCACAAGATGGCAATCACACTGCTACTGCAGCCGCAGCTTCTGGTTTTGCTGGAAGCAACGGTGAGGATGCCATCTTGCACAAGGAGGGCGATCCAGTAGAGAATAATAGGCTGGGGCTGGCCAGCATTTTCTTCAATGACATGGCCTGGACCAGGAAAGCCCTGCAGCAGTTGCTCCGAAGCCTTGGTTTCCAAATCGGCACGAGAAAAACATGAAGTAAGCATCAGAGACGCTGACCTGCAGTTTTGGCTGATCACACGGTCTCGCGGTGCCTTCATTCAAAACTCGAAAGCTTCTACATGCTGCAAACCTACAAGGAACAAGTGTATTGAGAACTGCAACAAAAAACCCTTTACGTTGCTAAGGTCTGCAACACCAGCAGTACTTGTTAGCAGTGCAAAAAGAGCAGGAAAGCTGTTAGCAGTGCAAGTATGTTTAGGACCAGTAATGACTAAGCTACCAAAAAACTCAATTAGTGAAATGCATATTGTGGTTAAATTTATCTGTTCGATAAATTCTGTAGAATACATCTAGAATAAGAGCCAGGAAAAGCTGAAAGTCTGGAGATTTGGCATAACATGTGCTCCATAACCACAACGTATAAGCTCAACCACATGTACTTTAATCCACCAGATTTACGTTCATTTCTCAATCATTTCAGTTGTAATGCTAATATGCTCTTGTTCTGGTGATCTAGTAAAAATGATGTCCATGATTTGCAATGCTTTCTTTAATTTACATAAGAAAGCTGGCAGCATTTAAAACATGTAAACGTGAAATATCAGCGTGGACATTCCACTTTAATTAAACTAACTTGTTTACAAATAACTGTATCCGTCAAGGAAACAGTCAATATTCATGACATACCCCTACCCCTACCTGTTTACAGCTATCCAACTACGCCAGTATCCTGTGAAGATTACAGCTTTGGTTGTATGGAGCAAACGGTCCAAGGTCAACAGAATCCACGATGCACCATGCCCAATTCTCTATAATTTATCACCTTCTAAACTCGGAGAGGCAAAAAACACTTTGCATGAAAATCATGGTTCAAGAGGCAGCCAGCCAACCATAATTGGA
CDS Sequence
  • >LOC_Os01g63810.1
    ATGGAAACCGCCGCGGCCGTGGTGTGCCGAGGCGGCGGGCTGCGCGCGCCGGCGCGTCGCGGTGGCAGTGACAGCAGCACGACGCGCGCGGGTGGCGTTGCTGCCTCCCCGGCGCCCGCCACCACCGCGCGACGGAGGCCGCTGCTCGTCGCGTCGCTCGGGGAGCCCCTCATCACGGCGCAACCCTTGTCGTCGTCCCTGGGAGATGGCGCCGCGGTACACGAGACGCTGGCTCGCTCCGATTCGGTGATCCCGTCGCTGAAGCCGTCTCACTGCGTTGATCACTCGGTCCAGGTTGACGCCGACGAGGAGACCGGCTCCACCAAGACACTCCCACCTCCTGATGACGTTCCTACGAAGACAGTGCACGTCAAATTCGTGCTGCAAAAGCGGTGCGCGTTCGGGCAGCGCTTCCTCGTCGTCGGCGACGTCGCGGCGCTCGGTCTCTGGAATCCGGCGAAGGCGGCTGCTCTGGATTGGTCCGAAGACCACGTGTGGACAGTGAAGAAGGAATTGCCAGCAGAAAGATCGATCGAGTTCAAGTTCTTGCTGCAAGATCGATCGGGACATGTCGAATGGCAGCACGGTCGTAACAGAATACTGCATGTAGCTGACACCTCGAACACCTTGATTGTCTGCGAGGACTGGGACGAGGCGAAGAATCAGCAAGTATCAGAAGAGATTGGTGACGCAGATGGGATATTTTCAGGAAGCGATGGCGTCTTCCAGGAAGATGAGCTTCAACTGGGTGAAGAACAAGAGACCAACAAGGGTGTGACTGTAGGTGTGGATGATGCAAAATCGGCTTTAGTCACTTATATTTACAGGGAAATGATGGGAGCAAACGACGCGATCCAGCCACAGTTAGCATTGGACAAACACCACAAGATTCCAGATGAGCTTAGTGGGGAGGCAAACATGGCAGCACAAGATGGCAATCACACTGCTACTGCAGCCGCAGCTTCTGGTTTTGCTGGAAGCAACGGTGAGGATGCCATCTTGCACAAGGAGGGCGATCCAGTAGAGAATAATAGGCTGGGGCTGGCCAGCATTTTCTTCAATGACATGGCCTGGACCAGGAAAGCCCTGCAGCAGTTGCTCCGAAGCCTTGGTTTCCAAATCGGCACGAGAAAAACATGA
  • >LOC_Os01g63810.2
    ATGGAAACCGCCGCGGCCGTGGTGTGCCGAGGCGGCGGGCTGCGCGCGCCGGCGCGTCGCGGTGGCAGTGACAGCAGCACGACGCGCGCGGGTGGCGTTGCTGCCTCCCCGGCGCCCGCCACCACCGCGCGACGGAGGCCGCTGCTCGTCGCGTCGCTCGGGGAGCCCCTCATCACGGCGCAACCCTTGTCGTCGTCCCTGGGAGATGGCGCCGCGGTACACGAGACGCTGGCTCGCTCCGATTCGGTGATCCCGTCGCTGAAGCCGTCTCACTGCGTTGATCACTCGGTCCAGGTTGACGCCGACGAGGAGACCGGCTCCACCAAGACACTCCCACCTCCTGATGACGTTCCTACGAAGACAGTGCACGTCAAATTCGTGCTGCAAAAGCGGTGCGCGTTCGGGCAGCGCTTCCTCGTCGTCGGCGACGTCGCGGCGCTCGGTCTCTGGAATCCGGCGAAGGCGGCTGCTCTGGATTGGTCCGAAGACCACGTGTGGACAGTGAAGAAGGAATTGCCAGCAGAAAGATCGATCGAGTTCAAGTTCTTGCTGCAAGATCGATCGGGACATGTCGAATGGCAGCACGGTCGTAACAGAATACTGCATGTAGCTGACACCTCGAACACCTTGATTGTCTGCGAGGACTGGGACGAGGCGAAGAATCAGCAAGTATCAGAAGAGATTGGTGACGCAGATGGGATATTTTCAGGAAGCGATGGCGTCTTCCAGGAAGATGAGCTTCAACTGGGTGAAGAACAAGAGACCAACAAGGGTGTGACTGTAGGTGTGGATGATGCAAAATCGGCTTTAGTCACTTATATTTACAGGGAAATGATGGGAGCAAACGACGCGATCCAGCCACAGTTAGCATTGGACAAACACCACAAGATTCCAGATGAGCTTAGTGGGGAGGCAAACATGGCAGCACAAGATGGCAATCACACTGCTACTGCAGCCGCAGCTTCTGGTTTTGCTGGAAGCAACGGTGAGGATGCCATCTTGCACAAGGAGGGCGATCCAGTAGAGAATAATAGGCTGGGGCTGGCCAGCATTTTCTTCAATGACATGGCCTGGACCAGGAAAGCCCTGCAGCAGTTGCTCCGAAGCCTTGGTTTCCAAATCGGCACGAGAAAAACATGA
Protein Sequence
  • >LOC_Os01g63810.1
    METAAAVVCRGGGLRAPARRGGSDSSTTRAGGVAASPAPATTARRRPLLVASLGEPLITAQPLSSSLGDGAAVHETLARSDSVIPSLKPSHCVDHSVQVDADEETGSTKTLPPPDDVPTKTVHVKFVLQKRCAFGQRFLVVGDVAALGLWNPAKAAALDWSEDHVWTVKKELPAERSIEFKFLLQDRSGHVEWQHGRNRILHVADTSNTLIVCEDWDEAKNQQVSEEIGDADGIFSGSDGVFQEDELQLGEEQETNKGVTVGVDDAKSALVTYIYREMMGANDAIQPQLALDKHHKIPDELSGEANMAAQDGNHTATAAAASGFAGSNGEDAILHKEGDPVENNRLGLASIFFNDMAWTRKALQQLLRSLGFQIGTRKT*
  • >LOC_Os01g63810.2
    METAAAVVCRGGGLRAPARRGGSDSSTTRAGGVAASPAPATTARRRPLLVASLGEPLITAQPLSSSLGDGAAVHETLARSDSVIPSLKPSHCVDHSVQVDADEETGSTKTLPPPDDVPTKTVHVKFVLQKRCAFGQRFLVVGDVAALGLWNPAKAAALDWSEDHVWTVKKELPAERSIEFKFLLQDRSGHVEWQHGRNRILHVADTSNTLIVCEDWDEAKNQQVSEEIGDADGIFSGSDGVFQEDELQLGEEQETNKGVTVGVDDAKSALVTYIYREMMGANDAIQPQLALDKHHKIPDELSGEANMAAQDGNHTATAAAASGFAGSNGEDAILHKEGDPVENNRLGLASIFFNDMAWTRKALQQLLRSLGFQIGTRKT*