Gene Details:
- MSU gene ID: LOC_Os01g63510
- RAPdb gene ID: Os01g0854500
- Gene Symbol: QHB OsQHB OsWOX5
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- During embryogenesis and crown root formation, QHB expression was observed prior to the morphological differentiation of the root.
- However, we detected different QHB expression patterns in the process of the RAM development, specifically between radicle and crown root formation, suggesting that the cell-fate determination of the QC may be controlled by different mechanisms.
- Using transformants carrying the QHB promoter-GUS and in situ hybridization, we found that QHB was specifically expressed in the central cells of a quiescent center (QC) of the root.
- Thus, our findings provide new insights into the role of rice WOX gene family in rice development and salt tolerance, and suggest that OsQHB is a valuable target for improving rice production in environments characterized by salt stress.
- Mutation in OsQHB led to a decrease in plant height, tiller number, panicle length, grain length and grain width, and an increase in salt tolerance.
- OsQHB Improves Salt Tolerance by Scavenging Reactive Oxygen Species in Rice.
- Moreover, the OsQHB mutants have higher ROS-scavenging enzymes activities and lower accumulation of ROS and malondialdehyde (MDA) under salt stress.
- Transcriptome and qPCR analysis showed that reactive oxygen species (ROS) scavenging-related genes were regulated by OsQHB.
- The function of WOX5 in enhancing cytokinin-dependent shoot regeneration is evolutionarily conserved, as conditional overexpression of OsWOX5 on SIM profoundly enhanced shoot regeneration in rice callus.
Function-related keywords:
- crown , root , crown-root , development , grain , grain-length , panicle , tiller , salt , tolerance , salt-tolerance , salt-stress , stress , plant-height , tiller-number , grain-width , panicle-length , reactive-oxygen-species , shoot , callus
Literature:
- Isolation and characterization of a rice WUSCHEL-type homeobox gene that is specifically expressed in the central cells of a quiescent center in the root apical meristem . DOI: 10.1046/j.1365-313x.2003.01816.x ; PMID: 12904206
- Transcriptome comparison between pluripotent and non-pluripotent calli derived from mature rice seeds . DOI: 10.1038/s41598-020-78324-z ; PMID: 33277567
- OsQHB Improves Salt Tolerance by Scavenging Reactive Oxygen Species in Rice . DOI: 10.3389/fpls.2022.848891 ; PMID: 35599895
- Ectopic expression of WOX5 promotes cytokinin signaling and de novo shoot regeneration . DOI: 10.1007/s00299-022-02932-4 ; PMID: 36219248
Related News:
Gene Resources:
- NCBI ID: AK102203
- UniProt accessions:
Sequences:
cDNA Sequence
- >LOC_Os01g63510.1
AGCATTGCTTCTGATCAGAAGAAGATCCCGGAAGAGACCAAATCAGAAGCTAAACCTATCTACCACAAGGCCAAGTTACGTTAGACCGTTTTGATCAGTGAATTAGCTTTCAAGCAGTTCTACTGCTCCCAGCCGCTCGATCGATCGATCGATCAAACAAGGTAGCTAGCTAGCTCAGGATCAAATCTGGTTTGTCTACATATATAGGTAGTGATCGATCAGTCGGTCGGTCATGGAGGCTCTTAGCGGGCGAGTGGGGGTGAAGTGTGGGCGGTGGAACCCGACGGCGGAGCAGGTGAAGGTCCTGACGGAGCTGTTCCGAGCGGGGTTGCGGACGCCGAGCACGGAGCAGATCCAGCGCATCTCCACTCACCTCAGCGCATTCGGCAAGGTGGAGAGCAAGAACGTCTTCTACTGGTTCCAGAACCACAAGGCCCGCGAGCGCCACCACCACAAGAAGCGCCGCCGCGGCGCCTCCTCCCCCGACAGCGGCAGCAACGATGACGATGGCCGCGCCGCAGCCCACGAGGGCGACGCCGACCTCGTCCTGCAGCCTCCTGAGAGCAAGCGGGAGGCCAGAAGCTACGGCCATCATCACCGGCTCATGACATGCTACGTGAGGGATGTGGTGGAGACGGAAGCGATGTGGGAGCGGCCGACGAGGGAGGTGGAAACGCTGGAGCTGTTCCCACTCAAGTCGTATGACCTCGAGGTGGACAAGGTCCGGTATGTGAGGGGCGGCGGTGGCGAGCAGTGCAGGGAGATCTCGTTCTTCGACGTCGCTGCCGGCCGGGATCCGCCGCTGGAGCTCAGGCTTTGCAGCTTCGGCCTCTAGATCGACCCGTGCATTGATGTGCCGCACATATGGCATGAGATGCAGCTTGCCGCGACTGTACCTGGCCTCTTGTCGCTGTGCCTAGTCCTAGTTCTAGCTGCCCCACATCAAAAGGAGTACGTACGAGATGTCATGAGATACGGCATGTGTTGTGGGAAAGTCCGTTGTTGCATGCAGTATCTGGGAGGATCAGGAAAGAGCACGCAAACGAGATTTCCCCATTGCCCTTTTCCTTGGTCTTTCCATCATGAGATGTACTTCTACTTTTCTTCTTTTCTGCTACATGGATACTAACATGGTCACTGCTGATACTTCTATATGATATATCCATTAATTAATATTCCATATTCCTTTTT
CDS Sequence
- >LOC_Os01g63510.1
ATGGAGGCTCTTAGCGGGCGAGTGGGGGTGAAGTGTGGGCGGTGGAACCCGACGGCGGAGCAGGTGAAGGTCCTGACGGAGCTGTTCCGAGCGGGGTTGCGGACGCCGAGCACGGAGCAGATCCAGCGCATCTCCACTCACCTCAGCGCATTCGGCAAGGTGGAGAGCAAGAACGTCTTCTACTGGTTCCAGAACCACAAGGCCCGCGAGCGCCACCACCACAAGAAGCGCCGCCGCGGCGCCTCCTCCCCCGACAGCGGCAGCAACGATGACGATGGCCGCGCCGCAGCCCACGAGGGCGACGCCGACCTCGTCCTGCAGCCTCCTGAGAGCAAGCGGGAGGCCAGAAGCTACGGCCATCATCACCGGCTCATGACATGCTACGTGAGGGATGTGGTGGAGACGGAAGCGATGTGGGAGCGGCCGACGAGGGAGGTGGAAACGCTGGAGCTGTTCCCACTCAAGTCGTATGACCTCGAGGTGGACAAGGTCCGGTATGTGAGGGGCGGCGGTGGCGAGCAGTGCAGGGAGATCTCGTTCTTCGACGTCGCTGCCGGCCGGGATCCGCCGCTGGAGCTCAGGCTTTGCAGCTTCGGCCTCTAG
Protein Sequence
- >LOC_Os01g63510.1
MEALSGRVGVKCGRWNPTAEQVKVLTELFRAGLRTPSTEQIQRISTHLSAFGKVESKNVFYWFQNHKARERHHHKKRRRGASSPDSGSNDDDGRAAAHEGDADLVLQPPESKREARSYGHHHRLMTCYVRDVVETEAMWERPTREVETLELFPLKSYDLEVDKVRYVRGGGGEQCREISFFDVAAGRDPPLELRLCSFGL*