Gene Details:

• MSU gene ID: LOC_Os01g63420
• RAPdb gene ID: Os01g0853400
• Gene Symbol: OsCOI1 OsCOI1a
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• We silenced OsCOI1 in rice plants via RNA interference (RNAi) to determine the role of OsCOI1 in rice defense against rice leaf folder (LF) Cnaphalocrocis medinalis, a chewing insect, and brown planthopper (BPH) Nilaparvata lugens, a phloem-feeding insect.
• These results suggest that OsCOI1 is an indispensable signaling component, controlling JA-regulated defense against chewing insect (LF) in rice plants, and COI1 is also required for induction of TrypPI, POD and PPO in rice defense response to LF infestation.
• In wild-type rice plants (WT), the transcripts of OsCOI1 were strongly and continuously up-regulated by LF infestation and methyl jasmonate (MeJA) treatment, but not by BPH infestation.
• Thus, our study suggests that OsbHLH148 acts on an initial response of jasmonate-regulated gene expression toward drought tolerance, constituting the OsbHLH148-OsJAZ-OsCOI1 signaling module in rice.
• Silencing OsCOI1 did not alter LF-induced LOX activity and JA content, but it led to a reduction in the TrypPI content, POD and PPO activity by 62.
• MeJA application efficiently suppressed the expression of BR pathway genes, and this inhibition depended on the JA coreceptor OsCOI1.
• The oscoi2 mutants, but not the OsCOI1a and oscoi1b mutants, exhibited severely low fertility, indicating the crucial role of OsCOI2 in rice fertility.
• Transcriptomic analysis revealed that the transcriptional changes after methyl jasmonate (MeJA) treatment were moderate in the leaves of oscoi2 mutants compared to those in the wild-type or OsCOI1a and oscoi1b mutants.
• OsCOI1b is involved in root growth and grain-size control and plays overlapping roles with OsCOI1a in spikelet development, while OsCOI2 regulates leaf senescence, male sterility, root growth, and grain size.
• OsCOI2 binds to most individual OsJAZs in the presence of endogenous JA ligands, as OsCOI1a /1b do, albeit with greater partner selectivity.

Function-related keywords:

• brown-planthopper ,  insect ,  jasmonate ,  drought ,  leaf ,  defense-response ,  defense ,  ja ,  drought-tolerance ,  BR ,  JA ,  fertility ,  methyl-jasmonate ,  leaf-senescence ,  root ,  growth ,  spikelet ,  grain ,  grain-size ,  root-growth ,  male-sterility ,  spikelet-development

Literature:

• OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice .  DOI:  10.1111/j.1365-313X.2010.04477.x ;  PMID:  21332845
• silencing COI1 in rice increases susceptibility to chewing insects and impairs inducible defense .  DOI:  10.1371/journal.pone.0036214 ;  PMID:  22558386
• Involvement of OsJAZ8 in jasmonate-induced resistance to bacterial blight in rice .  DOI:  10.1093/pcp/pcs145 ;  PMID:  23104764
• Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade .  DOI:  10.1073/pnas.1201616109 ;  PMID:  22529386
• Jasmonic acid-mediated defense suppresses brassinosteroid-mediated susceptibility to Rice black streaked dwarf virus infection in rice .  DOI:  10.1111/nph.14376 ;  PMID:  27976810
• Dynamic Perception of Jasmonates by the F-Box Protein COI1 .  DOI:  10.1016/j.molp.2018.07.007 ;  PMID:  30092285
• OsJAZ13 Negatively Regulates Jasmonate Signaling and Activates Hypersensitive Cell Death Response in Rice .  DOI:  10.3390/ijms21124379 ;  PMID:  32575555
• Genome Editing Reveals Both the Crucial Role of OsCOI2 in Jasmonate Signaling and the Functional Diversity of COI1 Homologs in Rice .  DOI:  10.1093/pcp/pcac166 ;  PMID:  36472361
• Functional dissection of rice jasmonate receptors involved in development and defense .  DOI:  10.1111/nph.18860 ;  PMID:  36869435

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: