Gene Details:
- MSU gene ID: LOC_Os01g61380
- RAPdb gene ID: Os01g0829800
- Gene Symbol: OsMDH1
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- The OsMDH1 was expressed in different tissues of rice plants including leaf, leaf sheath, panicle, glume, bud, root, and stem and was induced in the presence of NaCl.
- Transient expression of OsMDH1-GFP in rice protoplasts showed that OsMDH1 localizes to chloroplast.
- In this study, we identified a new loss-of-function OsMDH1 mutant displaying salt stress-tolerant phenotype.
- Transgenic rice plants overexpressing OsMDH1 (OsMDH1OX) displayed a salt stress-sensitive phenotype.
- Moreover, the pyridoxine content was higher in the OsMDH1 mutant and lower in OsMDH1OX plants than in the NIP plants under the salt stress, indicating that OsMDH1 negatively regulates salt stress-induced pyridoxine accumulation.
- Together, our results establish a novel, negative regulatory role of OsMDH1 in salt stress tolerance by affecting vitamin B6 content of rice tissues.
Function-related keywords:
- leaf , chloroplast , stem , salt , tolerance , salt-stress , stress , stress-tolerance
Literature:
- Rice plastidial NAD-dependent malate dehydrogenase 1 negatively regulates salt stress response by reducing the vitamin B6 content . DOI: 10.1111/pbi.13184 ; PMID: 31161713
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os01g61380.1
GGGGACTCTCGGAGAGCGGTTCGAGGGGGCCGTCGTTTGCGAGGCCGCAAGTCGCCGTCGGCGGCCGTCGCCATCGCCGCAACATAAAAAAAGCGAGCAAACCCGTTGCTGCGCGCTCTGCGCTTCTGCCCTTTTACGACCGAAGAGGTAGCCAACCCACGCATCCCCCCCCTGACCTGCCCCCTCCACAGCCGCCGCCGCCGCCGCCGCTACGGCTCACGAGAGTACCGAACGATCTGCTGCCACCCCCAACCCAAGTGACCAACCGAAGGCGGAGGGTAGCAACTTCGGTCTGCTCATCCCAAGTTAGGCATGGCATCAACTGTTGCCATCAATTTGATTGGTGCTCAGGCTGGCATTATCTCAAAGTTAAGGAATTGCGACATCACAAGCTACAGTGGCTTAAAGGCAAGATCATCTATTAGTTTTGAGTCAAGGTCATCATTCCTGGGTCAGAATGCATCTCTTCGATCATCTATCTCTCCAAGGATTGTACCAAAGGCAAACTCTGGATCTCACATTTCTCCTGAGGCATCTTACAAAGTGGCAGTTCTCGGTGCTGCTGGTGGCATTGGTCAACCGTTGGGCTTGTTAATTAAGATGTCTCCTCTGGTCTCTGCACTACATCTGTATGATATTGCAAATGTCAAAGGAGTTGCTGCTGATCTGAGCCACTGCAACACACCTTCTCAGGTTCTGGACTTTACTGGGCCCTCGGAATTAGCCAACTGCTTGAAAGGTGTGGATGTTGTTGTCATCCCTGCTGGAGTCCCAAGGAAGCCAGGAATGACTCGTGATGATCTTTTTAACATCAATGCGAGCATTGTCAAGACACTTGTTGAGGCTGTTGCAGACAATTGTCCAGAAGCCTTTATCCATATCATTAGCAACCCAGTAAACTCAACAGTGCCCATTGCTGCTGAGGTTCTGAAACAGAAGGGTGTCTATAACCCCAAGAAGCTTTTCGGAGTTACCACCCTAGATGTTGTCAGAGCTAACACATTTATAGCTCAGAAGAAGAACCTGAAGCTCATTGATGTTGATGTCCCAGTTGTTGGTGGTCATGCTGGGATTACTATCCTACCATTGCTGTCAAAAACCAGACCATCTGTCACCTTCACAAATGAGGAAACTGAAGAGCTGACAAGGAGGATACAGAATGCTGGGACAGAGGTGGTGGAGGCGAAAGCTGGTGCTGGATCTGCTACCCTGTCGATGGCCTATGCAGCTGCCAGATTTGTTGAGTCATCCCTCCGTGCATTGGCTGGTGATCCAGATGTTTACGAGTGCACATTTGTTCAATCTGAATTAACTGAGCTGCTGTTCTTTGCATCTAGGGTTAAGCTTGGTAAGAATGGTGTTGAGTCCATCATTTCTTCCGACCTTGAGGGAGTGACTGAATATGAGGCCAAGGCCCTTGAGTCATTGAAGCCTGAGTTGAAGGCCAGCATTGAGAAGGGCATCGAGTTCGTCCACAAACAGCAGGCTGCCGCTACTTCTTGAAGTGTGGAGAATGACTATCGGCATGAGAGAAGATAATAAATGCAGAAATGGTTCTCAATAGCTTTTAGTTTTGAAGGCGGAATTTTTCGTGTCTAGTTGTTGCTTGTTTAGTCTCTTCTGATCACATGAGGGCGTGGATGCCCAAACTGTTGCCTGTTTTCATGAGCTATTTGACCCTTGTGCCTGCATCTTGATGAGATACACAAACTAAGGAATAAAGTAGTTTTTGATTGTTGCCTGATTTATGTTATTATATATA
CDS Sequence
- >LOC_Os01g61380.1
ATGGCATCAACTGTTGCCATCAATTTGATTGGTGCTCAGGCTGGCATTATCTCAAAGTTAAGGAATTGCGACATCACAAGCTACAGTGGCTTAAAGGCAAGATCATCTATTAGTTTTGAGTCAAGGTCATCATTCCTGGGTCAGAATGCATCTCTTCGATCATCTATCTCTCCAAGGATTGTACCAAAGGCAAACTCTGGATCTCACATTTCTCCTGAGGCATCTTACAAAGTGGCAGTTCTCGGTGCTGCTGGTGGCATTGGTCAACCGTTGGGCTTGTTAATTAAGATGTCTCCTCTGGTCTCTGCACTACATCTGTATGATATTGCAAATGTCAAAGGAGTTGCTGCTGATCTGAGCCACTGCAACACACCTTCTCAGGTTCTGGACTTTACTGGGCCCTCGGAATTAGCCAACTGCTTGAAAGGTGTGGATGTTGTTGTCATCCCTGCTGGAGTCCCAAGGAAGCCAGGAATGACTCGTGATGATCTTTTTAACATCAATGCGAGCATTGTCAAGACACTTGTTGAGGCTGTTGCAGACAATTGTCCAGAAGCCTTTATCCATATCATTAGCAACCCAGTAAACTCAACAGTGCCCATTGCTGCTGAGGTTCTGAAACAGAAGGGTGTCTATAACCCCAAGAAGCTTTTCGGAGTTACCACCCTAGATGTTGTCAGAGCTAACACATTTATAGCTCAGAAGAAGAACCTGAAGCTCATTGATGTTGATGTCCCAGTTGTTGGTGGTCATGCTGGGATTACTATCCTACCATTGCTGTCAAAAACCAGACCATCTGTCACCTTCACAAATGAGGAAACTGAAGAGCTGACAAGGAGGATACAGAATGCTGGGACAGAGGTGGTGGAGGCGAAAGCTGGTGCTGGATCTGCTACCCTGTCGATGGCCTATGCAGCTGCCAGATTTGTTGAGTCATCCCTCCGTGCATTGGCTGGTGATCCAGATGTTTACGAGTGCACATTTGTTCAATCTGAATTAACTGAGCTGCTGTTCTTTGCATCTAGGGTTAAGCTTGGTAAGAATGGTGTTGAGTCCATCATTTCTTCCGACCTTGAGGGAGTGACTGAATATGAGGCCAAGGCCCTTGAGTCATTGAAGCCTGAGTTGAAGGCCAGCATTGAGAAGGGCATCGAGTTCGTCCACAAACAGCAGGCTGCCGCTACTTCTTGA
Protein Sequence
- >LOC_Os01g61380.1
MASTVAINLIGAQAGIISKLRNCDITSYSGLKARSSISFESRSSFLGQNASLRSSISPRIVPKANSGSHISPEASYKVAVLGAAGGIGQPLGLLIKMSPLVSALHLYDIANVKGVAADLSHCNTPSQVLDFTGPSELANCLKGVDVVVIPAGVPRKPGMTRDDLFNINASIVKTLVEAVADNCPEAFIHIISNPVNSTVPIAAEVLKQKGVYNPKKLFGVTTLDVVRANTFIAQKKNLKLIDVDVPVVGGHAGITILPLLSKTRPSVTFTNEETEELTRRIQNAGTEVVEAKAGAGSATLSMAYAAARFVESSLRALAGDPDVYECTFVQSELTELLFFASRVKLGKNGVESIISSDLEGVTEYEAKALESLKPELKASIEKGIEFVHKQQAAATS*